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Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Samples were subsequently separated by SDS-PAGE and transferred to Immobilon-P polyvinylidene difluoride membranes (Millipore). Immunoblot analysis was performed with the antibodies indicated and visualization was achieved with the Immobilon Western Chemiluminescent HRP substrate (Millipore).
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bioRxiv - Microbiology 2021Quote: ... The cultures were then washed 3x in pre-warmed P-buffer containing 1% dimethylsulfoxide (DMSO, Sigma) to ensure removal of unbound dye molecules ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... 1% penicillin/streptomycin (P/S) (penicillin: Santa Cruz Biotechnology, Dallas, USA; streptomycin: Sigma-Aldrich, Munich, Germany), 2 mM glutamine ...
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bioRxiv - Genomics 2020Quote: ... solution containing 1% each of Nonidet P-40 and Tween 20 (Millipore Sigma, St. Louis, MO). Additionally ...
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bioRxiv - Physiology 2020Quote: ... mice were injected intraperitoneally with α-methyl-p-tyrosine (250 mg/kg α-MPT; Sigma-Aldrich), an active competitive inhibitor for TH ...
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bioRxiv - Neuroscience 2020Quote: ... cerebellum or hippocampus were resolved by SDS-PAGE and transferred onto PVDF Immobilon-P membranes (Millipore). After blocking with 5% nonfat dry milk in 1X TBS with 0.1% Tween-20 (TBST ...
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bioRxiv - Biochemistry 2020Quote: ... Ethereal alcoholic solutions of diazomethane were prepared from N-methyl-N-nitroso-p-toluenesulfonamide (Sigma-Aldrich). All samples were extracted with tert-methyl butyl ether and mixed with 50 μL of diazomethane solution ...
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bioRxiv - Neuroscience 2021Quote: ... The cell lysates were resolved by SDS-PAGE and transferred onto PVDF Immobilon-P membranes (Millipore). After blocking with 5% nonfat dry milk in TBS containing 0.1% Tween 20 for 1hr at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... purified P dimers were buffer exchanged to 150 mM ammonium acetate (Sigma-Aldrich, reagent grade ≥ 98%) at pH 7 via centrifugal filter units at 12000 g at 4°C (Vivaspin 500 ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... or developmental time course samples were transferred to Immobilon-P PVDF (#IPVH00010, 0.45 μm, Millipore-Sigma) membranes ...
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bioRxiv - Neuroscience 2020Quote: ... or developmental time course samples were transferred to Immobilon-P PVDF (#IPVH00010, 0.45 μm, Millipore-Sigma) membranes ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM EDTA and 1% Nonidet P-40) supplemented with protease inhibitor (Cocktail Set III; Millipore) for 10 mins on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5% Nonidet P-40) in the presence of 5μL protease inhibitor cocktail (Sigma-Aldrich’s. Louis, USA) and 1mM PMSF ...
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bioRxiv - Immunology 2020Quote: ... containing 10 % fetal bovine serum (FBS, Biochrom GmbH) and 1 % penicillin-streptomycin (P/S, Sigma-Aldrich). After 10 days ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μM carbonyl cyanide p-trifluoromethoxyphenylhydrazone (CCCP) and 2µM Antimicyn A / 1µM Rotenone (all Sigma-Aldrich). For each condition 3 measurements ...
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bioRxiv - Neuroscience 2020Quote: ... 1.0% Nonidet P-40) with protease and phosphatase inhibitor cocktails (Sigma-Aldrich, St. Louis, MO, USA) was used for the total protein fraction ...
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bioRxiv - Microbiology 2021Quote: ... Fresh cyanophage P-SSM2 lysate was concentrated with Amicon Ultra-15 30K Centrifugal Filter Units (Millipore) at 3,000 g for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... Bovine pancreas DNAse (DN-25) and papain (#P-4762) were from Sigma-Aldrich (St. Louis, MO). Protease inhibitor cocktail (Complete ...
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bioRxiv - Cell Biology 2020Quote: ... 10 µM ROCK inhibitor Y27632 (ATCC® ACS-3030™) and 1% P/S (Sigma Aldrich)) under agitation during 30 min at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... 60 mM of p-CA and 30 mM of MOPS (pH 7.0, Sigma Catalog Num. M1254) was used to test the performance of the strains ...
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bioRxiv - Neuroscience 2023Quote: ... p-S6-Ser235/236-Ser240/244 (1:1000) except anti-β-actin (Sigma-Aldrich; 1:10,000) used for loading control ...
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bioRxiv - Genomics 2023Quote: ... fractionated by SDS-PAGE (12% acrylamide gel) and transferred to a PVDF membrane (Immobilon-P, Millipore). Blots were developed using alkaline phosphatase-conjugated secondary antibodies (Sigma ...
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bioRxiv - Neuroscience 2022Quote: Rats were transcardially perfused with 4% paraformaldehyde (PFA) in 0.1M PBS (PBS tablets Sigma P-4417). Brains were immediately frozen in 2-methylbutane (isopentane ...
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bioRxiv - Pathology 2022Quote: ... followed by SDS-PAGE electrophoresis and transferred onto PVDF membranes (Immobilon-P, Millipore, Billerica, MA, USA). Blots were blocked by Tris-buffered saline-bovine serum albumin 1% and incubated with Rabbit pAb SAA1 (AB171030 ...
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bioRxiv - Biochemistry 2024Quote: ... before mounting with anti-fade solution (0.02% p-phenylenediamine [Sigma, P6001] in 90% glycerol in PBS). Cells were imaged on a Nikon Eclipse Ti2 confocal microscope ...
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bioRxiv - Immunology 2023Quote: ... the dermis was digested in 3 mL dermal dissociation solution (1 mg/mL Collagenase P (Sigma), 100 µg/mL DNAse I (Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Ba+ F-1-P was subjected to ion exchange chromatography to using a Dowex 50WX4 (Sigma) column pre-charged with 2 N HCl and equilibrated in ddH2O ...
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bioRxiv - Plant Biology 2023Quote: ... 1% PVPP) plus 10 μL of protease inhibitor cocktail (P-9599; Sigma, St. Louis, MO, USA). The homogenate was quickly transferred into a 1.5 mL tube and centrifuged at top speed for 30 seconds at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... biopsies were digested for 40min at 37°C in digestion solution (0.05% Collagenase P (Sigma-Aldrich), 100μg/ml Liberase (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Lysates migrated through 10 or 12% PAGE and transferred to PVDF membranes (Immobilon-P, EMD Millipore).
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bioRxiv - Biochemistry 2022Quote: ... Samples were resolved with 12% SDS-PAGE and transferred onto a PVDF membrane (Immobilon-P, Millipore). Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... (Z)-11-15 Pentacosene (11-P) was synthesized via Wittig reaction from bromotetradecane (Sigma-Adrich, Germany) and undecanal (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... This was measured using a colorimetric assay of enzyme activity (SIGMAFAST p-NPP Kit; Sigma Aldrich), which uses p-nitrophenyl phosphate (pNPP ...
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bioRxiv - Developmental Biology 2022Quote: ... and postnatal day [P]0) and fixed overnight in 10% neutral buffered formalin solution (Sigma, HT501128). E18.5 ...
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bioRxiv - Neuroscience 2022Quote: ... The microfluidic chips were coated with 20 μg/ml poly-L-lysine (Sigma, Cat# P-12374) and 20 μg/ml laminin (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and dissociated using 5 U/mL Collagenase P (11 213 873 001, Sigma Aldrich, Buchs, Switzerland) in PBS for 40 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 100 µl of the PLA specific substrate D-valyl- leucyl-lysine-p-nitroanilide dihydrochloride (Sigma-Aldrich) was added at a final concentration of 0.4 mM in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... and pancreatin from porcine pancreas 8 x USP (P/N: 7545) were purchased from Sigma Aldrich Inc. ...
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bioRxiv - Physiology 2024Quote: ... Activity of alkaline phosphatase was detected in the presence of 1.35% p-nitrophenyl phosphate (Sigma Aldrich) in 0.1 M TRIS buffer pH 8.0 and 0.01 M MgCl2 (Sigma Aldrich ...
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bioRxiv - Biophysics 2024Quote: P.69T and variants (5 μM each) were digested with 2 μg/ml proteinase K (Sigma) for 24 h at room temperature in 50 mM TrisHCl pH8.8 ...
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bioRxiv - Biophysics 2022Quote: ... Protein G (Sigma) was coupled at 10 µg/mL in the presence of 10 mM sodium acetate buffer pH 4.5 at 30 µL/min for 300 seconds until ∼3500-4000 RU was immobilized ...
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bioRxiv - Microbiology 2021Quote: ... Protein G (Sigma) was covalently coupled following activation ...
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bioRxiv - Biophysics 2020Quote: ... Protein G (Sigma) at 10 μg/mL was coupled in the presence of 10mM sodium acetate buffer pH 4.5 at 30 μl/min for 300 seconds in various channels ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Molecular Biology 2021Quote: ... Following the incubation with a secondary antibody with a linked horse radish peroxidase the proteins were visualized by applying Immobilon™ Chemiluminescent Substrate (Millipore) and detection on an X-ray film.
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bioRxiv - Biochemistry 2020Quote: ... on genomic DNA isolated from 3-week-old tissue-cultured plants as a template and then selected by northern blot analysis with a HT specific cDNA fragment as probe and subsequently by western blot analysis with antibodies specific to rat HT protein (Sigma-Aldrich).
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...