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Citations for Millipore Sigma :
2101 - 2150 of 10000+ citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
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bioRxiv - Plant Biology 2023Quote: ... An equal amount of protein was used for immunoblot analysis using an anti-HA antibody (1:1000 dilution, Sigma) and anti-cMyc antibody (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Protein labeling was done by incubation with appropriate primary antibodies as indicated (Supplementary Table S1) in 5% BSA (Sigma) at 4°C overnight ...
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bioRxiv - Genomics 2022Quote: ... SPA-tagged protein was pulled down from clarified lysates with an anti-FLAG antibody (Sigma-Aldrich, #F1804-1MG, RRID:AB_262044) bound to Protein A-Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436 ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-BRI2 proteins were immunoprecipitated with anti-FLAG mouse monoclonal antibody M2 cross-linked to agarose beads (Sigma A2220); immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799) ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Microbiology 2023Quote: ... The DNA in 50 µL of water was immunoprecipitated with 20 µg/mL anti-protein A antibodies (Sigma Aldrich) and purified by phenol/chloroform extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... and the eluted proteins were subjected to Western Blot analysis using a GST antibody (1:1000, Millipore 05-782).
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bioRxiv - Molecular Biology 2022Quote: ... The filters were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (07-181, Millipore), p-AMPK (#2535 ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the equivalent of 500-800 µg of protein was incubated with 1 µg of anti-GFP antibody (Sigma-Aldrich) and then incubated with Protein A/G magnetic beads (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The various 3xF tagged NRPD1 and CLSY proteins were detected using a monoclonal anti-FLAG-HRP antibody (Sigma #A8592). RDR2-eGFP was detected using an anti-eGFP polyclonal antibody68 ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...
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bioRxiv - Cell Biology 2024Quote: ... 3FLAG-6HIS-YFP-tagged proteins expressed from the endogenous locus were immunoprecipitated using anti-FLAG M2 antibodies (F3165, Sigma) (Unnikrishnan et al. ...
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bioRxiv - Biochemistry 2024Quote: ... and expression of the GFP-tagged protein was verified by western blot using an anti-GFP antibody (Sigma-Aldrich). Colonies with the highest expression for a given construct were used to inoculate 300 mL of YEPD media containing 200 µg/mL Zeocin and grown overnight with shaking (220 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 4µg of the appropriate antibody and 20µl of Magnetic protein A/G beads (Magnachip, EMD-Millipore, Billerica, MA, USA) were used for the IP step ...
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bioRxiv - Molecular Biology 2023Quote: The following commercially available antibodies were used to detect targeted proteins: anti-RBM17 (1:1500 dilution; HPA037478, Sigma-Aldrich), anti-SF3B1 (1:3000 dilution ...
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bioRxiv - Immunology 2023Quote: ... Proteins were subsequently transferred onto nitrocellulose membranes and immunoblot analysis was performed with following antibodies: HA (Sigma-Aldrich, H6908), FLAG (M2 ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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Local induction of IAA5 and IAA29 promotes DNA damage-triggered stem cell death in Arabidopsis rootsbioRxiv - Plant Biology 2022Quote: ... Chromatin bound to SOG1-Myc proteins extracted from whole seedlings was precipitated with anti-Myc antibody (clone 4A6, Millipore). To quantify the precipitated chromatin ...
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bioRxiv - Biochemistry 2022Quote: ... HMCES-3flag protein was detected by immunoblotting with anti-flag antibody (1:10,000 dilution in PBST; Sigma, F1804-200UG) and rat anti-mouse secondary antibody peroxidase conjugate (1:20,000 dilution in PBST ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Physiology 2024Quote: ... S-nitrosylated Cx43 proteins were detected using a mouse anti-connexin-43 antibody (#C8093, Sigma-Aldrich, dilution: 1:8,000). ImageJ software was used to analyze the intensity of the signal.
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bioRxiv - Neuroscience 2024Quote: ... The primary antibodies used to visualize protein-expression were the following: rabbit anti-Cre (1:3000, Novagen 69050-3), mouse anti-Cre (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... or with a monoclonal primary antibody against fibroblast surface protein (Sigma-Aldrich, St. Louis, MO, US, F4771, 1:20) in PBS/0.1% FBS or PBS/0.05% FBS and Tween 20 (for permeabilisation ...
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bioRxiv - Molecular Biology 2024Quote: ... Single cell clones were screened for loss of FASTKD5 protein by immunoblotting with a FASTKD5 antibody (Sigma, Table S1) and frameshift mutations were confirmed by genomic sequencing ...
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bioRxiv - Microbiology 2024Quote: ... GST and 3×FLAG-tagged proteins were detected with a rabbit polyclonal anti-GST antibody (1:2500, Sigma-Aldrich) and a mouse monoclonal HRP-conjugated anti-FLAG antibody (M2 1:10000 ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were detected after incubating with peroxidase conjugated secondary antibodies for 1 hour and 5min with ImmobilonR Western (Millipore) after 5x washes ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection of specific protein bands was accomplished using a rabbit monoclonal anti-GLRX3 antibody (1:1,000, HPA028941, Sigma-Aldrich), ferritin (1:1,000 ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIPs were performed with antibodies against the following epitopes/proteins: HSV (Sigma-Aldrich, H6030; 5 µl per 1000 µg), HA (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein segments bound to the peptide array were detected by incubating with an anti-Flag M2 antibody (Sigma-Aldrich), followed by a horseradish peroxidase-linked goat anti-mouse antibody (Jackson ImmunoResearch) ...
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bioRxiv - Bioengineering 2021Quote: ... 50 mL of blocking buffer was prepared by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 μL of 0.5 M pH 8.0 EDTA (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... The surface of the coverslip was activated by incubation with 22.5 μM of biotin-BSA (Sigma-Aldrich) for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... Cooled down channels were filled with 1 mg/mL biotin labelled bovine serum albumin (BSA, Sigma-Aldrich) in T50 buffer (10 mM Tris/Cl ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule seeds were immobilized by sequential incubation with: (i) 1 mg/ml BSA-biotin (A8549; Sigma-Aldrich), (ii ...
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bioRxiv - Cell Biology 2022Quote: ... Sodium hyaluronan (1.5MDa) was from Lifecore (Chaska, MN, USA) and hyaluronan biotin sodium salt was from Sigma. Casein blocking buffer (10× ...
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bioRxiv - Bioengineering 2020Quote: ... The unreacted biotin was removed by ultrafiltration with an Amicon column (30 KDa MWCO, Millipore, CAT: UFC5010BK).
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bioRxiv - Microbiology 2020Quote: ... RcsF-His (5mg/mL in 0.1 M MES [pH 5.5]) was incubated with Biotin-LC-Hydrazide (1.25 mM, final concentration; Sigma) and N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide (EDC ...
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bioRxiv - Neuroscience 2021Quote: ... Remaining surface biotin was cleaved by incubation with glutathione cleavage buffer (50 nM L-Glutathione (Sigma #G6013) 75 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... the glass bottom of a 384-well MatriPlate was coated with 1mg/mL biotin-BSA (Sigma-Aldrich), followed by 200µg/mL NeutrAvidin (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated with an IB4-biotin conjugate (1:200, Sigma-Aldrich, Burlington, MA, USA, L2140) prepared in 5% normal goat serum (NGS)/PBST overnight at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... The beads were then re-suspended in 20 μl of 2 μg/ml Atto565-biotin (Sigma-Aldrich), incubated for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 mM CTP, 1 mM GTP, 0.65 mM UTP, 0.35 mM Biotin-16-UTP, pH 7.5; Roche/Sigma) and ∼40 U of either T7 (Roche/Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... unreacted biotin-pentylamine was removed by ultrafiltration with a 3 or 10 kDa MWCO membrane (Merck Millipore). The recovered upper residual liquid (∼100 μL ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... a 96 well glass bottom plate was prepared by plating first biotin-BSA (Thermo) and fibronectin (Sigma), followed by neutravidin (Thermo) ...
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bioRxiv - Immunology 2023Quote: ... Biotin-poly-L-lysine was produced by incubating 5 mg/mL poly-L-lysine hydrobromide (Sigma P6282) with 1.5 mM EZ-Link Sulfo-NHS-Biotin (TFS 21217 ...