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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 0.5 mg TTN5 protein was combined with 1 U of agarose bead-coupled alkaline phosphatase (Sigma Aldrich, Germany) for degradation of bound GDP to GMP and Pi in the presence of 1.5-fold molar excess of non-hydrolyzable GTP analog GppCp (Jena Bioscience ...
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bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were used at the following dilutions: rat anti-HA epitope IgG1 (clone 3F10; Sigma) at 1:5,000 ...
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bioRxiv - Physiology 2019Quote: ... RT-qPCR was carried out as previously described [20] using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes (see Supp Table 1 for primer list) ...
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bioRxiv - Physiology 2020Quote: ... Afterwards the rats were anaesthetized with urethane (1.5 g/kg b.w. i.p., Sigma-Aldrich, Poland) and were subjected to hemodynamic measurements including ABP and LVP recordings as described above for SHHF and SD rats.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... rats received intra-cerebral saline or d-amphetamine (10 or 30 µg/hemisphere; Sigma-Aldrich, Dorset ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded on plates coated with 50 µm/ml rat tail collagen I (Sigma).
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bioRxiv - Synthetic Biology 2022Quote: ... Nonspecific antibody binding was blocked by incubation with 20 µg/ml Rat IgG (Sigma, I4131) for 5 min ...
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bioRxiv - Plant Biology 2021Quote: ... After incubation with HRP-conjugated secondary goat antibody against rabbit or rat primary antibodies (Sigma), detection was performed with the Clarity Max Western ECL substrate (#1705062 ...
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bioRxiv - Molecular Biology 2021Quote: ... Nonspecific antibody binding was blocked by incubation with 10μg/ml Rat IgG (Sigma #I-8015) for 15 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibodies used were as follows: peroxidase-conjugated goat anti-rat HRP conjugate (Millipore) / goat anti-rabbit HRP conjugate (Millipore ...
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bioRxiv - Bioengineering 2021Quote: ... samples were stained with 20 µg/ml mouse anti-rat αvβ3 integrin antibody (Millipore Sigma) on ice for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were then conditioned to associate the foot shocks with the odour acetophenone (Sigma Aldrich). The odour ...
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bioRxiv - Genetics 2019Quote: ... MIN6 mouse insulinoma cells79 and 823/13 rat insulinoma cells80 were cultured in DMEM (Sigma) supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... RH or RHtgd057-HA were stained with rat anti-HA primary antibody (Sigma, clone 3F10) at 1:500 dilution ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... rats received 0.05 ml of a glycerol locking solution containing 3 parts glycerol (Sigma-Aldrich, St.
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bioRxiv - Physiology 2021Quote: Rice rats were injected SC with declomycin and calcein (Sigma Chemical Co., St Louis, MO) at a dose of 15 mg/kg on the 7th and 2nd days before sacrifice ...
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bioRxiv - Neuroscience 2021Quote: Rats were lightly anesthetized with isoflurane and 100 µL of Complete Freund’s adjuvant (CFA; Sigma) was injected into the left hindpaw ...
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bioRxiv - Bioengineering 2022Quote: ... Briefly, the rat received (intraperitoneally, IP) 120 mg/kg caffeine (Millipore Sigma, catalog number C0750) and 60 mg/kg dobutamine (Cayman ...
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bioRxiv - Neuroscience 2022Quote: Rats were trained to orally self-administer a 2.5% sucrose (Sigma, Saint-Quentin-Fallavier, France) solution as described for the operant ethanol self-administration ...
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bioRxiv - Immunology 2022Quote: ... Cells were cultured in collagen coated (rat tail, type I, Sigma-Aldrich, Saint Louis, USA) culture flasks in growth medium (EGM-2 MV medium (Lonza ...
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bioRxiv - Biochemistry 2022Quote: ... Rats in the EAE group were immunized with subcutaneous injections of 100 µg MBP (Sigma) emulsified in 5 mg/mL complete Freund’s adjuvant (CFA ...
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bioRxiv - Physiology 2022Quote: ... Rats were also paralyzed with pancronium bromide (2 mg/kg; Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2023Quote: Brain sections from sham-control and STN-lesioned rats were stained with thionine (Sigma-Aldrich) to assess the location and extent of STN lesions ...
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bioRxiv - Immunology 2023Quote: ... Rat polyclonal anti-mouse IgM antibody was conjugated onto cyanobromide activated Sepharose beads (Sigma Aldritch) as per the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... rats were anesthetized with urethane (1.4 g/kg in 0.9% NaCl, i.p. ethyl carbamate, Sigma); the depth of anesthesia was regularly checked by pinching the feet and additional doses were administered as needed ...
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bioRxiv - Neuroscience 2024Quote: ... the same mice were retested 30 min after an IP injection of rat αCGRP (Sigma). The testing duration is 45 min/mouse ...
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bioRxiv - Immunology 2021Quote: Egg white immunoglobulins (300 μl) were purified using a commercially available protein A affinity chromatography kit (PURE-1A, Sigma Aldrich Co.). The manufacturer’s instructions were followed ...
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bioRxiv - Biophysics 2021Quote: CoA levels in the protein solution after proteinase K digestion were quantified using a commercial CoA assay kit according to manufacturer’s protocol (Sigma-Aldrich; MAK034). CoA concentration is determined by an enzymatic assay ...
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bioRxiv - Microbiology 2019Quote: Ribonucleoprotein complex immunoprecipitation (RIP) was performed using the EZ-Magna RIP RNA-binding protein Immunoprecipitation Kit (Merck Millipore Billerica, Massachusetts, USA). Huh7.5-SGR cells were seeded in Petri dishes at 106 cells/dish for 24 h prior to transfection with pKSRP-HA plasmids ...
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bioRxiv - Microbiology 2019Quote: Interactions between the SRB1 and NS1 proteins from DENV and ZIKV in Huh-7 and C6/36 cells were detected using the commercial kit Duolink (Sigma-Aldrich) based on in situ proximity ligation assay (PLA ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Cell Biology 2022Quote: The assay was performed using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (# C10428, Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... ∼2×107 parasites were collected by centrifugation (1,200 x g for 10 min) and pull-downs were performed using the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Sigma-Aldrich). Unenriched ‘input’ samples were generated by reserving back 10% of the starting lysate ...
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bioRxiv - Microbiology 2023Quote: ... The interactions between the two labeled proteins were detected using the Duolink in Situ Red Starter Kit Mouse/Rabbit (#DUO92101-1kit, Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Biochemistry 2023Quote: ... One half was used for protein extraction and the other half used for UA quantification using a UA Assay Kit (MAK077, Sigma-Aldrich). Cells were disrupted mechanically ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein quantification was performed using the BCA protein quantification assay (QPro BCA kit, Cyanagen) against a bovine serum albumin (BSA) calibration curve (Sigma Aldrich).
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bioRxiv - Plant Biology 2024Quote: ... To quantify the concentration of purified protein in the samples a Bicinchoninic acid (BCA) assay was conducted using the BCA kit following the manufacturer recommendations (Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
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bioRxiv - Cell Biology 2021Quote: ... precleared cell extracts were incubated with the indicated antibody for 4 hours at 4°C with rotation followed by 1 hour of pull-down by 1:1 protein A/G agarose beads (GE, 17061801, 17127901) or FLAG/Myc beads (FLAG resin: Sigma, A2220; Myc resin: Sigma, E6654). Immunoprecipitates were washed with lysis buffer three times before electrophoresis.
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bioRxiv - Microbiology 2021Quote: ... The aqueous phase was transferred to a new tube containing linear polyacrylamide (Gene-EluteTM LPA, Sigma). To allow RNA precipitation ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001). After 24 to 40 hours of transfection cells were mounted and imaged on an Olympus FV1000 confocal microscope (40x Uplan FL N oil objective ...
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bioRxiv - Cell Biology 2021Quote: Gene knockdown was performed as described previously 43 with the following siRNAs purchased from Sigma-Aldrich:
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bioRxiv - Cell Biology 2022Quote: ... and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001). After 24 to 40 hours of transfection cells were mounted and imaged on a Nikon A1 confocal microscope (Plan Fluo 40x oil objective ...
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bioRxiv - Microbiology 2022Quote: ... esiRNAs targeting a different region of the gene were employed (esiSec; Sigma-Aldrich; See Table S3). Two different esiRNAs targeting CryAB and PFD2 were tetsted ...
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bioRxiv - Microbiology 2019Quote: ... Genes were cloned by ligation between BamHI and NotI sites of a modified pET28a vector (Novagen) yielding N-terminally His6-tagged small ubiquitin-like modifier (SUMO ...
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bioRxiv - Microbiology 2019Quote: ... Genes or loci of interest were PCR amplified with KOD Xtreme Hot Start polymerase (Millipore Sigma) using the primers listed in Table S6 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The SARS-CoV-2 nsp7 and nsp8 genes were codon optimized and cloned into pET46 (Novagen) with an N-terminal 6x histidine tag ...