Labshake search
Citations for Millipore Sigma :
2301 - 2350 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2020Quote: ... secondary antibody was added (Goat anti-rabbit HRP, 1% milk, TBST 0.2%, 2 h, RT, 1:5000; Sigma). Once washed ...
-
bioRxiv - Neuroscience 2020Quote: ... SYSY), vesicular glutamate transporter 2 (VGLUT2; 1:1,000, SYSY) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:50,000, Millipore) at 4 °C for 16 h ...
-
bioRxiv - Plant Biology 2019Quote: ... the T1 seed selection was done on 1/2 MS medium + 10 mgL-1 Glufosinate-ammonium (Sigma-Aldrich). For construct containing the FASTR screenable marker ...
-
bioRxiv - Cancer Biology 2019Quote: ... 4 µl of SUV TBS solution (1:1 ratio of SUV and 2× TBS solution, TBS form Sigma) was placed in each hole of the device ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 plus 5 μM Blebbistatin (Bleb, B0560, Sigma), pelleted at 150g for 5 minutes ...
-
bioRxiv - Physiology 2019Quote: ... The powdered food mix was added in a 1:1 ratio (grams:mL) to a 2% agar (Sigma #A1296) solution in water before solidification to a semi-solid consistency that lessens the potential for hoarding of food in group housing of mice and increases accuracy of measuring food consumption as large chunks can’t be taken at one time and the food doesn’t crumble like with standard rodent chows ...
-
bioRxiv - Genomics 2021Quote: ... 10 mM MgCl2,10 mM Tris pH 7.4, 1 mM CaCl2) with increasing amounts (0, 0.5, 1, 2, 5, 7.5, 10 U) of MNase (Sigma) at 30°C for 10 min ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... DMEM without serum contained 1 µg of L-1- tosylamide-2-phenylethyl chloromethyl ketone-treated trypsin (TPCK; Sigma) for activation of the virus infectivity ...
-
bioRxiv - Physiology 2023Quote: ... the gonads were incubated overnight in the dark at 4 °C with secondary goat anti-rabbit IRDye 680RD-conjugated antibody (LiCor) 1:1000 in PBST/0.1% BSA and 1 ng/µl 4,6-diamidine-2-phenylindole (DAPI, Sigma). The gonads were mounted on 2% agarose pads in Fluoromount-G (ThermoFisher ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by incubation of secondary antibodies (1:500) for 2 h and DAPI (1:10,000, D9542, Sigma-Aldrich) in PBS for 5 min at RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
-
bioRxiv - Bioengineering 2023Quote: ... b-mercaptoethanol and 1000 U ml-1 LIF) and 2 ug ml-1 doxycycline (cat. 33429 Sigma-Aldrich). MEFs were reprogrammed in the presence of doxycycline for 12 days and without doxycycline for the last 5 days ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 with 5 µM Blebbistatin (Bleb, B0560, Sigma), and pelleted at 150 g for 5 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... the viral medium was removed and the wells overlaid with a 1:1 solution of 2% agar (Sigma) and 2X DMEM containing 5% (v/v ...
-
bioRxiv - Neuroscience 2024Quote: LL-341070 was provided by Autobahn Therapeutics as a solution in 1% 1-methyl-2-pyrrolidone (Sigma 270458) and 1% Kolliphor HS 15 (Sigma 42966 ...
-
bioRxiv - Bioengineering 2021Quote: ... The yeasts were grown in yeast extract (1%, w/v) peptone (2%, w/v) glucose (2%, w/v) (YPD) media supplemented with 50mg/L ampicillin (Sigma-Aldrich, 69534). The strains were grown in 250 mL Erlenmeyer flasks containing 100 mL of YPD ...
-
bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
-
bioRxiv - Molecular Biology 2020Quote: ... sections of mouse heart were perfused for 1-2 min with 2ml of 2% TTC (Sigma-Aldrich Co., St. Louis, MO, USA) in phosphate-buffered saline (PBS ...
-
bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
-
bioRxiv - Plant Biology 2023Quote: ... Yeast cells were grown in rich medium (YPD; 1% yeast extract [Difco, 10215203], 2% peptone [Difco, 211705], and 2% glucose [wt/vol]) (Sigma-Aldrich, G7021) on an orbital shaker (200 rpm ...
-
bioRxiv - Biochemistry 2022Quote: ... were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt, Aldrich) or negatively charged 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS, Sigma-Aldrich, Inc) containing N,N’-methylenebisacrylamide (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... were resuspended 2 mL of viral particle supernatant for E:T of 2:1 with 8 μg/mL of Polybrene (Millipore Sigma, Burlington, MA), maintained in at 37°C for 4 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... medium supplemented with 10 mM of 2-(4-(2-hydroxyethyl) piperazin-1-yl) ethane sulfonic acid (HEPES; Sigma-Aldrich, St. Louis, MO), 24 mM sodium bicarbonate ...
-
bioRxiv - Plant Biology 2019Quote: ... Sections were stained for 2 h in PBS containing 2 µg/ml DAPI and mounted in a 1:1 mix of glycerol and water containing 1 % DABCO antifade (Sigma-Aldrich, USA) for confocal imaging using a Zeiss (Cambridge ...
-
bioRxiv - Microbiology 2020Quote: ... 49 with 1.5% weight vol−1 agar supplemented with 2 mg mL−1 5-Fluoroorotic acid (USBiological, USA) and 5 µg mL−1 uracil (Sigma–Aldrich USA) in order to select for plasmid excision ...
-
bioRxiv - Cell Biology 2022Quote: ... 2-hydroxy-4’-(2- hydroxyethoxy)-2-methylpropiophenone (I2959) (Sigma-Aldrich, USA), a photoinitiatior (0.5% w/v ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2% (v/v) 2-mercaptoethanol and 2 mM PMSF) (Sigma-Aldrich) and homogenize ...
-
bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with avertin (2, 2, 2-Tribromoethanol (Sigma-Aldrich), 125-250 mg/kg ...
-
bioRxiv - Developmental Biology 2022Quote: ... using 1:4 phosphate buffer from the Total SOD kit (Sigma-Aldrich, kit 19160) for SOD activity ...
-
bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
-
bioRxiv - Cell Biology 2020Quote: ... pLKO-Puro shHDAC4 1 (TRCN0000314667) and 2 (TRCN000004832) were obtained from Sigma-Aldrich. pWZL-Hygro-HDAC4PAM (V31L or P16A) ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 mM EDTA and 1% NP40) supplemented with Protease Inhibitor Cocktail (Sigma-Aldrich). Proteins (25-50µg ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 × 100 U/mL penicillin-streptomycin and 0.05 mM 2-Mercaptoethanol (Sigma-Aldrich). At 168 h ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were blocked for 1 h in 2% bovine serum albumin (BSA) (Sigma)/PBS solution at RT ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples were diluted 1:2 for RT-qPCR using SYBR Green Mastermix (Sigma) and primers detailed in Table 3.
-
bioRxiv - Molecular Biology 2021Quote: ... selected for 2-3 weeks with 1 µg/ml of puromycin (Sigma- Aldrich), and surviving cells were fixed with methanol and stained with Giemsa (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... 2 mM L-glutamine and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, USA) and maintained at 37°C and 5% CO2 in a humidified atmosphere ...
-
bioRxiv - Microbiology 2019Quote: ... Egg water was supplemented with 0.003% PTU (1-phenyl-2-thiourea) (Sigma-Aldrich) after bacterial injections had been performed ...
-
bioRxiv - Bioengineering 2019Quote: ... blocked for 1 hour in a 2% bovine serum albumin (Sigma-Aldrich A4503) and 0.1% Tween 20 solution in PBS ...
-
bioRxiv - Bioengineering 2019Quote: ... with 10 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Sigma-Aldrich) and 2% FBS ...
-
bioRxiv - Neuroscience 2019Quote: ... anti-microtubule associated protein 2 (MAP2, 1:500, mouse monoclonal, Sigma, St.Louis, MO), GluN2B (1:1000 ...
-
bioRxiv - Immunology 2019Quote: Larvae (2 dpf) were embedded in 1% low melting point agarose (Sigma Aldrich), and image acquisition was performed by using a Leica TCS SP8 confocal microscope (Leica Microsystems ...
-
bioRxiv - Genetics 2021Quote: ... embryos and larvae were treated with 0.003% 1-phenyl 2-thiourea (Sigma, P7629) to inhibit pigment synthesis ...
-
bioRxiv - Cell Biology 2020Quote: ... and 1% phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044, Sigma-Aldrich) added fresh] or RIPA buffer [25 mM Tris pH 7.5 ...
-
bioRxiv - Microbiology 2021Quote: ... Serial 1:2 dilutions of a standard solution of nisin Z (Sigma-Aldrich) were prepared in 2xTY and 100 µl were distributed in 96-well plates (Sarstedt ...
-
bioRxiv - Neuroscience 2020Quote: ... were made in 1-methyl-2-pyrrolidinone and α,β-methylene ATP (Sigma) was made in water ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse mAb anti-α-Tubulin (Sigma-Aldrich, clone B-5-1-2, #T5168); mouse mAb anti-Flag M2 (Sigma-Aldrich #F1804) ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-Centrin-2 (1:250, clone 20H5, 04-1624, Merck Millipore), rabbit polyclonal anti-WDR90 (1:250 ...
-
bioRxiv - Bioengineering 2021Quote: ... 242.6 mg of 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Sigma-Aldrich) was dissolved in 1 L of deionized water with a pH value that was adjusted to 8.0 to make 1 mM HEPES buffer solution ...