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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... After a saturation step with Gamma Immune (Sigma Aldrich, dilution 1 :2), cells were labeled with antibodies detecting the following cell-surface markers ...
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bioRxiv - Biochemistry 2023Quote: ... A solution of graphene oxide (GO, 2 mg mL−1, Sigma-Aldrich) was bath sonicated to evenly resuspend it ...
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bioRxiv - Cell Biology 2023Quote: ... Treatment with HU (Sigma-Aldrich, 0, 0.5, 1, 2, 4, 8 mM) was for 24h ...
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bioRxiv - Developmental Biology 2023Quote: ... The cDNA was diluted 1:2 in nuclease free water (SIGMA-Aldrich) and real time quantitative PCR (RT- qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM PMSF and phosphatase inhibitors cocktails 2 and 3 (Sigma-Aldrich). Lysates were cleared by centrifugation at 17000 x g for 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1% filter sterilized oyster glycogen (9005-79-2, Sigma-Aldrich), maltotriose (J66491.03 ...
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bioRxiv - Developmental Biology 2024Quote: ... Blocking was performed for 1 hour in 2% Donkey Serum (Sigma, D9663), 0.1% BSA (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Sigma H0887). These cells were chosen as an experimental system as previously described (Massacci et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... was supplied at 1 mM for 2 hr and MG-132 (Sigma) at 10 μM for 2 hr ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Plant Biology 2021Quote: ... Five days after germination the plant were incubated in liquid in ½ MS with 1% sucrose media with or without 50 µM 2-bromopalmitate (2-BrP; Sigma-Aldrich), and with or without 100 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... were then transferred to 2 mL microtubes containing 1 mL of lysis buffer [2% (m/v) cetyltrimethyl ammonium bromide (Sigma Aldrich), 1.4 M NaCl ...
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bioRxiv - Immunology 2020Quote: PBMC or Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-1/2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Strains were streaked from frozen stock onto yeast extract peptone dextrose (YPD; 1% yeast extract, 2% peptone, 2% dextrose, Sigma-Aldrich) agar and left overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Plant Biology 2022Quote: ... 0.85 % saline (2 mins), Phosphate Buffered Saline (PBS) (2 mins), 0.125 mg ml-1 Pronase (37°C, 10 mins) (Sigma-Aldrich, P2308), 0.2 % Glycine (2 mins ...
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bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed with PBST and then incubated for 2 h at room temperature in PBS with 2 μg mL−1 DAPI (D9542, Sigma-Aldrich). Cells were imaged using an LSM980 confocal microscope (Zeiss) ...
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bioRxiv - Cell Biology 2023Quote: ... on ice in LCMS-grade acetonitrile/methanol/water (2:2:1 v/v) with 1nmol scyllo-inositol internal standard per sample (Sigma Aldrich). Sample debris was then removed via centrifugation and polar metabolite extracts were dried using a SpeedVac Vacuum Concentrator ...
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bioRxiv - Biophysics 2023Quote: Crude and low-dense MVs were exposed to SCL medium supplemented with CaCl2 (2 mM) and either 2 mM Na2HPO4 or 1 mM adenosine triphosphate (Sigma-Aldrich). Mineralization was carried out at 37°C and using samples with fixed 50 µg of total protein/mL concentration ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Bioengineering 2020Quote: ... Serial dilutions of the samples (1:2 to 1:64) and a standard row containing 1 to 40 nmol Neu5Ac (Sigma Aldrich) were prepared in extraction buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed cells were then incubated overnight at 4°C in primary antibody (GFP 1:2000, Abcam ab13970, γH2A.X 1:500, Sigma Aldrich 05-636; Map-2 1:250, Sigma Aldrich M4403 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA, 0.5 mM DTT, 1 mM NaF, 1 mM PMSF and 1% Protease Inhibitor Cocktail from Sigma, pH 7.4) for 30 min on ice ...
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bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The fresh tissue was dissected followed by digestion for 2 hours at 37°C in 1 mg ml−1 Collagenase 1 (Sigma-Aldrich). Debris was removed and cells isolated by sequentially passing through 100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were ground in an ice-cold buffer (50 mmol.L-1 TRIS, 5 mmol.L-1 EDTA, 2 mmol.L-1 DTT, protease inhibitor cocktail (Sigma, St. Louis, MO, USA)) ...
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bioRxiv - Developmental Biology 2023Quote: ... 90%, 100%), incubated in acetone/Spurr resin (1:1, 30 minutes, 1:2, 30 minutes) and in Spurr resin (Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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bioRxiv - Cancer Biology 2022Quote: We analyzed chemotherapy responses of human leukemia cell lines expressing wild-type NT5C2 or NT5C2 mutant constructs following 72-hour incubation with increasing concentrations of 6-mercaptopurine by measuring metabolic reduction of the tetrazolium salt MTT using Cell Proliferation Kit I (Sigma-Aldrich, #11465007001) following manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... GLP-1 and GIP-1 EIA kit was purchased from Sigma-Aldrich. Nitroblue tetrazolium chloride ...
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bioRxiv - Immunology 2022Quote: A 96 well ELISA plate was coated with bovine fetuin (5 μg/mL; Sigma) in carbonate buffer overnight ...
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bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
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bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
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bioRxiv - Molecular Biology 2021Quote: ... 2-Deoxy-D-glucose (2-DG; 2 mM, Sigma-Aldrich).
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bioRxiv - Microbiology 2021Quote: ... then washed and cultured one week in normal cell culture medium and passaged in 10 cm dishes seeded with 3×105 cells using culture medium supplemented with 30 μM 6-thioguanine (6-TG, Sigma). Cells were also plated without 6-TG to determine plating efficiency ...
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bioRxiv - Genetics 2020Quote: ... Transduction was carried out by infecting 2x105 hES H1 cells per well of a 6-well plate with lentiviruses in the presence of 6 μg/ml Polybrene (Sigma) and 10 μM Y-27632 ROCK inhibitor ...
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bioRxiv - Developmental Biology 2022Quote: ... Transduction was carried out by infecting 2×105 hES H1 cells per well of a 6-well plate with lentiviruses in the presence of 6 μg/ ml Polybrene (Sigma) and 10 μM Y-27632 ROCK inhibitor ...
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bioRxiv - Microbiology 2022Quote: ... Some of the groups were then treated with 100μM 6-aminonicotinamide (6-AN) or dehydroepiandrosterone (DHEA) (both from Sigma-Aldrich), two pentose phosphate pathway inhibitors18 ...
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bioRxiv - Cell Biology 2023Quote: ... the newly formed bundles were kept in standard 2D muscle progenitor proliferation medium containing 1.5 mg/mL 6-aminocaproic acid (6-ACA) (Sigma-Aldrich). After 48 hours ...
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bioRxiv - Developmental Biology 2023Quote: C57BL/6 ES and C57BL/6-tdTomato+ ES cell lines were cultured on feeder cells with 2il medium: DMEM (Millipore) supplemented with 15% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: mRNA was purified from total RNA with 2 rounds of GeneElute mRNA purification kit (Sigma). rRNA was removed using Ribominus kit (Invitrogen ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... and Phospho/Total ERK 2-Plex Magnetic Bead Kit (EMD Millipore Corporation, Billerica, MA, U.S.A.) were used to assess the levels of these proteins ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then blocked in 1% BSA for 20 minutes before being incubated in a primary antibody against acetylated α tubulin (1:4000 dilution, clone 6-11B-1, Sigma-Aldrich, St. Louis, MO, USA), GFP (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies used in this study were: anti-acetylated α-tubulin (Antibody registry ID: AB_477585, clone 6-11B-1, mouse IgG2b, Sigma, diluted 1:250 in blocking solution), anti-SV2 (AB_2315387 ...
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bioRxiv - Evolutionary Biology 2019Quote: Five skate embryos were cultured from stage 23–30 in 900 mL of salt water (Instant Ocean) with/without all-trans RA (Sigma; final concentration of 2 × 10−6 M). After the culture ...
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bioRxiv - Physiology 2021Quote: ... The resulting CX3CR1CreERT2(+/−)::Ptger4fl/fl offspring were injected at 6 weeks of age with 2 doses of tamoxifen (T5648, Sigma, 4 mg s.c., prepared in corn oil) 48 hours apart ...