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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 2% charcoal-stripped FBS and EPC cocktail (1 µM MPA, Sigma Cat#1378001- 200MG ...
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bioRxiv - Neuroscience 2023Quote: ... for 2h before clearing in benzyl alcohol:benzyl benzoate (1:2) (Sigma-Aldrich-Merck). Samples were stored in this solution at room temperature and protected from light.
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bioRxiv - Molecular Biology 2023Quote: ... 2 to 3 million cells were cross-linked using 1% formaldehyde (Sigma, F8775) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044). Lysates were cleared at 17,000g ...
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bioRxiv - Cell Biology 2024Quote: ... fracture tissues were digested with 1mg/ml Collagenase 1/Collagenase 2 (Sigma-Aldrich) and 2 mg/ml dispase II (Roche) ...
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bioRxiv - Physiology 2024Quote: ... Liver (50mg) was extracted in 2:1 Chloroform: Methanol mixture (Chloroform: Sigma Aldrich, C2432-1L ...
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bioRxiv - Neuroscience 2021Quote: ... the electrode surfaces of 6-well Axion plates (Axion Biosystems, CytoView MEA 6) were coated with 10 mg/mL poly-D-lysine (Sigma, P7280) at room temperature overnight ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were resuspended in 6 mL of cold water and lysed by adding them dropwise to 6 mL of boiling 8% SDS (Sigma-Aldrich) within 10 min ...
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bioRxiv - Microbiology 2022Quote: ... the msfGFP levels were normalized for OD600 and converted to absolute units using 5(6)-carboxyfluorescein (5(6)-FAM) (Sigma Aldrich) as a calibrant (36 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on Greiner Bio-One CELLSTAR Polystyrene 6-well Cell Culture Multiwell Plates coated first with Poly-L-ornithine hydrobromide (6 μg/mL in 1xPBS (Sigma-Aldrich), 1 h at 37℃ ...
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bioRxiv - Microbiology 2023Quote: ... The relative msfGFP measurements were normalised for their respective OD600 values and subsequently converted to absolute units of the calibrant 5(6)-carboxyfluorescein (5(6)-FAM)) (Sigma-Aldrich). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... 500’000 cells per well were seeded as single cells onto new 6-well plates (6 WPs) coated with vitronectin and cultured in mTESR+ medium supplemented with thiazovivin (SML1045, Sigma Aldrich). 2 μL of each viral construct (TetO-Ngn2-P2A-puromycin ...
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bioRxiv - Cell Biology 2019Quote: ... the slides with attached intact axonemes were treated overnight at 4°C with blocking buffer containing the diluted primary antibody (mouse anti-acetylated tubulin, Sigma-Aldrich, clone 6-11B-1, 1:1000). On the next day ...
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bioRxiv - Cell Biology 2022Quote: ... Leptin and NPY were measured using commercially available ELISAs (all from Millipore, Temecula, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: ELISA was performed using conditioned media (∼80% confluent cells, concentrated with Millipore UFC 900324 filters) and normalized by protein concentration (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed and blocked with 5% Foetal Bovine Serum (FBS, Sigma, F9665) in 1x PBS/0.1% Tween 20 for 1 hour at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Leptin content in media was analyzed by ELISA (Cat. # EZML-82K, Millipore Sigma, Danvers, MA).
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were captured in ELISA plates precoated with 5 μg/ml of avidin (Sigma) in Na2CO3-NaHCO3 buffer (pH 9.6 ...
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bioRxiv - Immunology 2024Quote: ... Nunc MaxiSorp ELISA plates were precoated with dsDNA (100 mg/mL, Sigma, St. Louis, MO) in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: ... and VEGF concentrations were measured by ELISA (R&D Systems, Mercodia, Abcam, and Sigma, respectively), and total plasma bile acids using a colorimetric assay (Abcam) ...
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bioRxiv - Synthetic Biology 2023Quote: ... were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) for 1hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mM NaF, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 1 mM PMSF, 75 mU/ml aprotinin-Sigma-, 50 mM Tris-HCl, pH 7.5) containing 1% TX-100 ...
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bioRxiv - Neuroscience 2021Quote: ... frozen VMH (from 2 – 3 mice) was homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma) with Protector RNAase Inhibitor (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Microbiology 2020Quote: ... Strains were routinely grown under yeast conditions (media at 30°C) in YPG (1% yeast extract, 2% Bacto peptone, 2% glucose) or under filament-inducing conditions using RPMI medium (Sigma, St. Louis, MO) with MOPS (morpholinepropanesulfonic acid ...
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bioRxiv - Plant Biology 2021Quote: ... total soluble protein was extracted from 50 mg of ground frozen shoot material in 250 µl 2× Laemmli buffer supplemented with 1% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich, Steinheim, Germany). Proteins were denatured for 10 min at 90°C and separated using 10% (w/v ...
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bioRxiv - Physiology 2021Quote: Muscle fibers were impaled with 2 sharp microelectrodes filled with 2 M potassium acetate solution containing 1 mM sulforhodamine 101 (Sigma-Aldrich, Catalogue #S7635) to allow for visualization ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (2% bovine serum albumin; Sigma Aldrich A4503 + 0.1% Tween20) and subsequently incubated in primary antibody solution (1:200 CD31 Dako IS610 + 1:200 CD10 Invitrogen PA5-85875 or 1:200 anti-laminin Abcam ab11575 or 5 μg/mL ZO-1 Invitrogen #61-7300 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were infected at low MOI 1-2 in EBM-2 media with supplements in the presence of 8 μg/mL polybrene (Sigma-Aldrich, St.Louis, USA) and spinoculation at 450g for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Mia PaCa-2 cells and PCAFs were then transduced with the respective viral suspension at different multiplicity of infection (MOI) in the presence of 6 µg mL-1 hexadimethrine bromide (polybrene, Sigma H9268). Cells were sorted for mCherry and EGFP fluorescence using a BD FACSAria™ Cell Sorter (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... BAT was then incubated for 1 hour at 37 °C before stimulation with 10 μM isoproterenol (ISO, Sigma, 54750-10-6). Samples were taken at the indicated time points ...
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bioRxiv - Plant Biology 2019Quote: ... protoplasts were isolated from 6-days-old protonemal cultures by incubation for 30 min in 1 wt% driselase (Sigma-Aldrich, D8037) dissolved in a 8.5 wt% mannitol solution in water ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated 12 min with 5% Triton in PBS and then primary cilia and SMO were stained using a mouse anti-acetylated tubulin Ab (6-11B-1, Sigma-Aldrich) and a rabbit anti-SMO Ab (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Electroporated PBMCs were transferred into 1 well of a 6 well plate seeded with MEFs in PBMC recovery medium (RPMI supplemented with 200uM 1-thioglycerol (Sigma M6145), 1uM Dexamethasone (Sigma D1756) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were counted and plated at a density of 1 x 106 cells per well of a 6 well plate that was pre-coated with sterile 1% (w/v) gelatin (Sigma-Aldrich). For immunocytochemistry ...
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bioRxiv - Microbiology 2020Quote: Seven-week old Arabidopsis leaves with or without herbivore infestation for 6 h were stained with 1 mg/ml DAB-HCl solution (Sigma, D5637) overnight in darkness ...
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bioRxiv - Cell Biology 2021Quote: HEMs or MNT1 cells seeded in a 6-well plate at 50-80% confluency were lysed in 1% Triton X-100 (T8787, Sigma-Aldrich) and centrifuged at 14,000 RPM for 30 min at 4°C to separate solubilized protein from melanin ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 0.1 M sodium cacodylate buffer containing 1% OsO4 and 1.5% K4Fe(CN)6 (Sigma-Aldrich, St. Louis, MO), en bloc stained with 2% aqueous uranyl acetate ...
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bioRxiv - Evolutionary Biology 2021Quote: Total bacterial counts were determined by plating out 25 µl of a dilution series ranging from 10−1 to 10−6 on LB-agar plates (Sigma-Aldrich). To identify recipient strains (carrying the dTomato marker ...
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bioRxiv - Biochemistry 2021Quote: ... cells were fixed using a 6% (v/v) formaldehyde:phosphate-buffered saline solution and stained with 1% (w/v) crystal violet (Sigma-Aldrich, Italy) in 70% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and 20 uL of 120 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (Pierce; dissolved in 47% ACN, 47% water and 6% HPLC-grade pyridine (Sigma Aldrich)) were added ...
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bioRxiv - Molecular Biology 2020Quote: ... To stabilize beta-catenin and to induce canonical Wnt signaling the cells were treated with 1 μM BIO (6-Bromoindirubin-3′-oxime, SIGMA B1686) for 24 hours before harvesting.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were passaged to single cell suspension and plated to achieve 65,000 cells per well of a 6-well plate in Nutristem supplemented with 1:2,000 Y-27632 (ROCK inhibitor; Sigma Aldrich, Y0503) [23] ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in StemFlex containing 1 μM H1152 (Millipore, #555550). On the next day ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and the pellet resuspended in 1 ml of BHI containing 10 µM 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFDA-SE, Sigma #21888). The suspension was incubated on a rotating shaker at 37°C and 200 rpm for 45 minutes ...
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bioRxiv - Physiology 2020Quote: ... On day 4 cells were re-stimulated with the same concentrations of anti-CD3/CD28 for 6 hours at 37°C in the presence of 1 μg/ml brefeldin-A (Sigma-Aldrich). As a control ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Cas9 viral supernatant was added to 1×106 cells growing in a 6-well plate plate in 3mL of complete media with polybrene (4mg/mL) (Sigma, H9268). The cell-virus mixture was centrifuged at 3000rpm at 25°C for 1h followed by incubation at 37°C overnight ...
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bioRxiv - Genetics 2021Quote: ... The reaction was performed in the presence and absence of an NADPH generating system: 1 mM glucose-6-phosphate (Sigma Aldrich), 0.1 mM NADP+ (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Filtered particles were either immediately introduced to cells (105 target cells plated in a 6-well plate) in antibiotic-free culture medium supplemented with 8 μg ml-1 polybrene (TR-1003-G, EMD Millipore) or stored in single use aliquots at −80ºC ...