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Citations for Millipore Sigma :
2301 - 2350 of 4180 citations for 6 Propylamino 7 deazapurine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 3 x 10 min 100% EtOH) and cleared (3 x 5 min) in xylene (247642, CAS: 1330-20-7, Sigma Aldrich). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... They were then incubated in primary antibodies (supplementary table 7) diluted in Odyssey blocking buffer supplemented with 0.2% (v/v) Tween 20 (Sigma-Aldrich, P9416) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... albicans cells were grown in RPMI-MOPS medium buffered to pH 7 (1,04% RPMI with glutamine and phenol red, without sodium bicarbonate (Sigma Aldrich, R6504), 3,45% MOPS ...
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bioRxiv - Microbiology 2023Quote: ... ∼8 x 106 parasites were pelleted by centrifugation at 800 rcf for 7 min and resuspended in FCS-free HMI-9 media with 1 µg/mL Hoechst 33342 (Sigma-Aldrich). Cells were pelleted again ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were seeded in 15-well IBIDI angiogenesis chambers during passaging and fixed 7-12 days later in 4% paraformaldehyde (PFA) (Sigma-Aldrich) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 3-Hexanol (CAS #623-37-0, purity >97%, Fluka, Buchs, Switzerland) and Heptanal (CAS #111-71-7, purity >95%, SAFC, Sigma Aldrich) (Table 1) ...
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bioRxiv - Physiology 2023Quote: ... The pelleted cells of the cardiac myocyte fraction were resuspended in ice-cold 7 mL Perfusion Buffer and applied onto a 20-µm nylon net membrane (Millipore, SCNY00020). Trapped cells on the membrane were collected with 7 mL Perfusion Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... pTyr (clone 4G10, 05-321), and HA (clone HA-7, H3663) and rabbit polyclonal anti-SEPTIN12 (HPA041128) were from Sigma-Aldrich. Rabbit and mouse monoclonal anti-DYKDDDDK (clone D6W5B ...
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bioRxiv - Systems Biology 2023Quote: PMA differentiated THP-1 cells were transiently transfected with 100nM targeted siRNA using low molecular weight polyethyleneimine (Sigma-Aldrich, 40872-7). Further ...
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bioRxiv - Microbiology 2023Quote: ... pestis KIM6+ outer membranes were examined by quantifying the β-lactamase activity as described previously (7) using the chromogenic substrate CENTA (Sigma). Briefly ...
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bioRxiv - Biochemistry 2023Quote: Female mice (n=5-7 per group) were fasted overnight and then injected with poloxamer 407 intraperitoneally (1 g/kg BW) (Sigma, 16758). Plasma TG concentrations were measured at 0 ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine monocyte/macrophage RAW264.7 subline LR5 (38) was cultured in RPMI 1640 medium (Mediatech) supplemented with 10% heat-inactivated newborn calf serum (Sigma-Adrich) and antibiotics ...
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bioRxiv - Genomics 2023Quote: ... total cellular ERAP2 protein was enriched by immunoprecipitation and incubated with L-Arginine-7-amido-4-methylcoumarin hydrochloride (R-AMC, A2027, Sigma-Aldrich). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... On day 7 of differentiation cells were incubated with tetramethlyrhodamine (TMRM) for 1 hour (concentration 80nM) and Hoechst (concentration 10nM) (Sigma Aldrich). Cells were incubated at 370C during this time ...
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bioRxiv - Plant Biology 2023Quote: Proteasome activity was measured by spectrofluorometry using the 7-amino-4-methylcoumarin (AMC)-labeled fluorogenic substrate succinate-LLVY-AMC (Roche Sigma-Aldrich) in cleared extracts of leaf 1 at different dpg (Üstün and Börnke ...
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bioRxiv - Systems Biology 2023Quote: ... single cells were sorted based on their GFP signal into 384-well plates containing 100 nl of unique CEL-seq2 compatible primer (7 ng/µl) and 5 µl mineral oil (Sigma-Aldrich). Following cell lysis and denaturation ...
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bioRxiv - Bioengineering 2023Quote: ... the surfaces of interest were incubated with a sterile filtered solution of 2 mg/mL dopamine (Dopamine 62-31-7, Sigma Aldrich) in 10 mM Tris-HCl (pH 8.5 ...
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bioRxiv - Cell Biology 2023Quote: ... MPP4 (7 x 104 cells per well) were sorted directly into 8-well plates pre-coated with poly-lysine (Sigma-Aldrich) and containing XF DMEM medium ...
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bioRxiv - Microbiology 2023Quote: ROS production within protozoan food vacuoles was investigated by staining cells with the ROS indicator 2’,7’-Dichlorofluorescein diacetate (DCFH-DA, Sigma Aldrich). After deacetylation by cellular esterases ...
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bioRxiv - Biochemistry 2023Quote: ... SsoPox variants were used at 1 mg.mL-1 in Tris buffer (50 mM Tris-HCl, pH 7) and with 1X SYPRO® orange (Sigma-Aldrich). Denaturation was followed using FRET channel ...
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bioRxiv - Neuroscience 2023Quote: We used the odorants methyl hexanoate (MHXE, CAS: 106-70-7) and 2-heptanone (HEPN, CAS: 110-43-0) (Sigma Aldrich). Odorant stimuli were generated with a custom-made stimulator optimized for temporal precision in binary mixtures [13] ...
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bioRxiv - Neuroscience 2023Quote: Fly brains from L3 larvae or 5–7-day old adult flies were dissected in ice-cold insect media (Schneiders media) (Sigma Aldrich) and fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2023Quote: ... mice and rats (male Sprague-Dawley, aged 7-10 weeks) were subcutaneously injected with the VEGF-receptor inhibitor SU5416 (Sigma-Aldrich) at a dosage of 20 mg/kg body weight under isoflurane anesthesia ...
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bioRxiv - Cell Biology 2023Quote: ... cells were split into 2 x 4 ml cultures and either fixed straight away with 3.7% formaldehyde (final concentration) or subjected to treatment (200 ng/ml rapamycin or 0.5 mg/ml calcofluor white (Sigma-Aldrich 910090)) for 1 hour ...
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Cell Biology 2023Quote: LPS- and OGD-treated cells were washed with PBS and stained with 5 μM 2′,7′-dichlorofluorescin diacetate (DCFH-DA; Sigma-Aldrich) for 20 min at 37°C to detect cell reactive oxygen species (ROS) ...
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bioRxiv - Neuroscience 2023Quote: ... seven tree shrews (TS 1–TS 7) were used to determine the appropriate dose of MPP+ (MPP+ iodide, D048, Sigma, USA) required for PD model development ...
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bioRxiv - Synthetic Biology 2023Quote: ... beginning on day 7 post-partum dams were administered twice-daily a 200ug dose of Bromocriptine (2mg/mL bromocriptine mesylate (Sigma; 1076501) and 2mg/mL tartaric acid (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell suspensions were mixed with 350 µl of freshly prepared lysis buffer (2M NaOH, 7% β-mercaptoethanol) and 350µl of 50% TCA (Sigma, T9159). After spin ...
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bioRxiv - Biochemistry 2024Quote: Reaction mixes were prepared with the desired ⍺β-tubulin concentrations (7 μM or 10 μM) containing 5% Alexa 568-tubulin plus 1 mg/ml BSA (Sigma) in assay buffer (BRB80 with 30 mM KCl ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded in a 96-well plate at a density of 15,000 cells per well in 50 µL of medium containing the HDAC substrate N-(4-Methyl-7-aminocoumarinyl)-Nα-(t-butoxycarbonyl)-Nω-acetyllysineamide (21 μM, MAL, Sigma-Aldrich) and incubated for 8 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: Eight-week-old WT and MCfosB- mice were sensitized with an intraperitoneal injection of 5 μg of anti-DNP IgE (SPE-7, Sigma-Aldrich) in 100 μL of sterile saline ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in 693 μL of buffer A and 7 μL of 10% digitonin (0.1% final concentration; Sigma-Aldrich, D141) were added to facilitate permeabilization of the cells for 5 min at 30 °C and mixed with the pipette several times during the incubation ...
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bioRxiv - Immunology 2024Quote: ... cFibs cultured on Matrigel-coated cell culture plates were harvested and stained for Anti-Fibroblast Antibody Clone TE-7 (Millipore Sigma) and Antibody to Sarcomeric Myosin MF20 (University of Iowa Developmental Studies Hybridoma Bank ...
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bioRxiv - Biophysics 2021Quote: ... 10 units of Glucose-6-phosphate Dehydrogenase from baker’s yeast (G6PDH; S. cerevisiae; Sigma-Aldrich) and either 0 ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected after 48 h using 6 µg/ml Blasticidine S (Sigma Aldrich) for 2 weeks.
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 10 μM 6-Diazo-5-oxo-L-norleucine (DON, Sigma Aldrich), 1 μM Palbociclib (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... The nuclei were stained by incubation with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted 1:10,000 in 1% BSA for 15min ...
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bioRxiv - Cell Biology 2020Quote: ... cdc12-6 cultures were shifted to 37°C and treated with 200 mM hydroxyurea (Sigma) for 1 h to protect cells from subsequent phototoxicity during imaging (Howell et al. ...
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bioRxiv - Cell Biology 2020Quote: ... ii) washed in PBS and resuspended by rotating with 6 mg human IgG (Sigma, I4506) for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... and resuspended in HBSS with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/ml, Sigma) for flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were counterstained with 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, cat. D8517, Sigma-Aldrich) diluted 1:3000 in PBS 1X ...
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bioRxiv - Developmental Biology 2021Quote: ... 4% PFA fixed zebrafish embryos were embedded in 6% low-melting agarose (LMA, Sigma-Aldrich) dissolved in 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... 3 mg of each compound was dissolved in 6 mL hexane (Sigma-Aldrich #139386-500ML) and 0.5 mL was pipetted into individual 2 mL glass vials fitted with 9mm PTFE lined caps (Agilent Crosslab ...
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bioRxiv - Cell Biology 2021Quote: ... was used to stain filamentous actin and 4′,6-Diamido-2-Phenylindole (DAPI, Sigma-Aldrich) to stain nuclei.
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed as before and stained with 4’,6-diamidino-2-phenylindole (DAPI) (SIGMA) followed by cover-slipping with Fluoro-gel with Tris buffer (Electron Microscopy Sciences).
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 4’,6-diamidino-2-phenylindole (D9542-10MG DAPI, Sigma Aldrich, Denmark) to stain DNA followed by 5 times wash in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... IL-6 and TNFα using Milliplex Map Mouse Serum Adipokine Panel (Millipore catalogue # MADKMAG-71K) on a Luminex 200IS platform.
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma, 1 µg/mL) and the cells were washed three times with PBS ...
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bioRxiv - Genomics 2020Quote: ... 20000 cells were directly lysed in 6 μL of 0.17% TrionX-100 (T8787, Sigma-Aldrich) for 3 min at 72 °C ...