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Citations for Millipore Sigma :
2151 - 2200 of 3947 citations for 6 Propylamino 7 deazapurine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... SKBR3 cells were starved for 24 h in serum-free medium and then stimulated for 15 min with 7-15 nM HRG-β1 (Sigma Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... The resultant pellets were resuspended in 100 μl of 1X PBS then incubated with 7 μl of 4% (w/v) phosphotungstic acid (Sigma-Aldrich) in 0.2 M magnesium chloride ...
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bioRxiv - Microbiology 2021Quote: ... The remaining mosquitoes were maintained under optimal conditions in the insectary and their midgut dissected on day 7 PBM to determine prevalence and load of infection by counting oocysts on mercurochrome (0.4% w/v- Sigma-Aldrich-M7011) stained midguts using a light microscope (X20 & X40).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... University of Nottingham) and Cos-7 (ATCC) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich or Thermo Fisher Scientific) supplemented with 10 % fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2019Quote: ... we repeated our measurements after 30 min superfusion in 10−7 M TTX and 10−5 M picrotoxin (PTX; Sigma-Aldrich). PTX blocks glutamatergic synapses in the STG of both C ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by SDS-PAGE in a BioRad mini gel system (7 × 8.4 cm) and transferred onto polyvinylidene fluoride membrane (EMD Millipore, IPFL00010). The membrane was blocked with TBS-T (20mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: Human leukaemia cells HL-60 and breast cancer cell line MCF-7 were cultured in RPMI and DMEM media (Sigma-Aldrich), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... and 500 μL) were added to each well along with a no virus control with 7 μg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... 2003): Notch1-CreERT2/R26mTmG mice of 5-7 months of age received a single intraperitoneal injection of Azoxymethane (AOM, Sigma #A5486) followed by Dextran Sulfate Sodium (DSS ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated as described in each legend respectively (MG132 – Sigma M7449; 5Z-7-oxozeaenol – Sigma O9890; TAK-715 – MedChem Express HY-10456; nocodazole – Sigma M1404). Equal volume DMSO was applied to cells as a vehicle control for compounds dissolved in DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were introduced in RPE-1 cells using Fugene HD transfection reagent and cells were selected based on GFP-expression or using 7 μg/mL puromycin (Sigma Aldrich) for 5 days.
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bioRxiv - Microbiology 2020Quote: ... parasites were maintained below ~7×105 cells per ml for up to 5 days prior to addition of brain heart infusion (BHI) broth (Sigma Aldrich).
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bioRxiv - Microbiology 2019Quote: Interactions between the SRB1 and NS1 proteins from DENV and ZIKV in Huh-7 and C6/36 cells were detected using the commercial kit Duolink (Sigma-Aldrich) based on in situ proximity ligation assay (PLA ...
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bioRxiv - Immunology 2021Quote: ... CD14+ monocytes were differentiated to MDMs by culturing for 7 days (at a concentration of 1 × 106 cells ml−1) in RPMI 1640 (Sigma, R8758) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: Lentiviral particles encoding guide RNA and Cas9 were produced in HEK 293T cells using mouse Irf7 CRISPR lentiviral transfer construct targeting exon 7 (target ID #MM0000202587) of Irf7 (Sigma Aldrich). Lenti CRISPR universal non-target plasmid was used as a control (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... that contain two parallel channels separated by a porous membrane (7 μm pores) were first perfused and incubated with 1% 3-Aminopropyltrimethoxysilane (Sigma, 281778) in ethanol for 1 hour and then incubated in an 80°C oven overnight ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Physiology 2020Quote: α-synuclein aggregates in the head muscles of 7-days old worms were visualized by fluorescence microscopy (400x magnification) in worms anesthetized with 15 mM sodium azide (Sigma, S2002). Pictures were segmented using Ilastik (version 1.3.0 ...
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bioRxiv - Biophysics 2022Quote: ... to which a 5 % of fluoro-surfactant was added (1H,1H,2H,2H-Perfluoro-1-octanol, CAS Nr 647-42-7, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... 7×103 cells were resuspended in 100μL of appropriate cell-culture media containing 1μg/mL hydrocortisone (Sigma, H0888, lot no. SLBG4963V) and 5μg/mL insulin (Sigma ...
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bioRxiv - Genomics 2022Quote: ... head kidney and spleen of 30 challenge fish were collected at three timepoint (pre-infection, 7 dpi and 14 dpi) into TRI Reagent (Sigma, UK) and stored at −80°C until RNA extraction.
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bioRxiv - Microbiology 2022Quote: ... 100-200 μl culture aliquots were transferred to 2 ml Eppendorf tubes with perforated lids followed by addition of 7 μM (10 μg/ml) polymyxin B (Sigma-Aldrich) or 31 μM (30 μg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... hCAI with the K978C mutation (h^K978C) or for T2A mutagenesis were designed using SnapGene software (SI, Table 7) and synthesised by Sigma Aldrich.
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bioRxiv - Neuroscience 2022Quote: ... 3 x 10 min 100% EtOH) and cleared (3 x 5 min) in xylene (247642, CAS: 1330-20-7, Sigma Aldrich). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... They were then incubated in primary antibodies (supplementary table 7) diluted in Odyssey blocking buffer supplemented with 0.2% (v/v) Tween 20 (Sigma-Aldrich, P9416) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... albicans cells were grown in RPMI-MOPS medium buffered to pH 7 (1,04% RPMI with glutamine and phenol red, without sodium bicarbonate (Sigma Aldrich, R6504), 3,45% MOPS ...
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bioRxiv - Microbiology 2023Quote: ... ∼8 x 106 parasites were pelleted by centrifugation at 800 rcf for 7 min and resuspended in FCS-free HMI-9 media with 1 µg/mL Hoechst 33342 (Sigma-Aldrich). Cells were pelleted again ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were seeded in 15-well IBIDI angiogenesis chambers during passaging and fixed 7-12 days later in 4% paraformaldehyde (PFA) (Sigma-Aldrich) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 3-Hexanol (CAS #623-37-0, purity >97%, Fluka, Buchs, Switzerland) and Heptanal (CAS #111-71-7, purity >95%, SAFC, Sigma Aldrich) (Table 1) ...
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bioRxiv - Physiology 2023Quote: ... The pelleted cells of the cardiac myocyte fraction were resuspended in ice-cold 7 mL Perfusion Buffer and applied onto a 20-µm nylon net membrane (Millipore, SCNY00020). Trapped cells on the membrane were collected with 7 mL Perfusion Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... pTyr (clone 4G10, 05-321), and HA (clone HA-7, H3663) and rabbit polyclonal anti-SEPTIN12 (HPA041128) were from Sigma-Aldrich. Rabbit and mouse monoclonal anti-DYKDDDDK (clone D6W5B ...
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bioRxiv - Systems Biology 2023Quote: PMA differentiated THP-1 cells were transiently transfected with 100nM targeted siRNA using low molecular weight polyethyleneimine (Sigma-Aldrich, 40872-7). Further ...
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bioRxiv - Microbiology 2023Quote: ... pestis KIM6+ outer membranes were examined by quantifying the β-lactamase activity as described previously (7) using the chromogenic substrate CENTA (Sigma). Briefly ...
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bioRxiv - Biochemistry 2023Quote: Female mice (n=5-7 per group) were fasted overnight and then injected with poloxamer 407 intraperitoneally (1 g/kg BW) (Sigma, 16758). Plasma TG concentrations were measured at 0 ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine monocyte/macrophage RAW264.7 subline LR5 (38) was cultured in RPMI 1640 medium (Mediatech) supplemented with 10% heat-inactivated newborn calf serum (Sigma-Adrich) and antibiotics ...
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bioRxiv - Genomics 2023Quote: ... total cellular ERAP2 protein was enriched by immunoprecipitation and incubated with L-Arginine-7-amido-4-methylcoumarin hydrochloride (R-AMC, A2027, Sigma-Aldrich). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... On day 7 of differentiation cells were incubated with tetramethlyrhodamine (TMRM) for 1 hour (concentration 80nM) and Hoechst (concentration 10nM) (Sigma Aldrich). Cells were incubated at 370C during this time ...
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bioRxiv - Plant Biology 2023Quote: Proteasome activity was measured by spectrofluorometry using the 7-amino-4-methylcoumarin (AMC)-labeled fluorogenic substrate succinate-LLVY-AMC (Roche Sigma-Aldrich) in cleared extracts of leaf 1 at different dpg (Üstün and Börnke ...
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bioRxiv - Systems Biology 2023Quote: ... single cells were sorted based on their GFP signal into 384-well plates containing 100 nl of unique CEL-seq2 compatible primer (7 ng/µl) and 5 µl mineral oil (Sigma-Aldrich). Following cell lysis and denaturation ...
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bioRxiv - Bioengineering 2023Quote: ... the surfaces of interest were incubated with a sterile filtered solution of 2 mg/mL dopamine (Dopamine 62-31-7, Sigma Aldrich) in 10 mM Tris-HCl (pH 8.5 ...
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bioRxiv - Cell Biology 2023Quote: ... MPP4 (7 x 104 cells per well) were sorted directly into 8-well plates pre-coated with poly-lysine (Sigma-Aldrich) and containing XF DMEM medium ...
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bioRxiv - Microbiology 2023Quote: ROS production within protozoan food vacuoles was investigated by staining cells with the ROS indicator 2’,7’-Dichlorofluorescein diacetate (DCFH-DA, Sigma Aldrich). After deacetylation by cellular esterases ...
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bioRxiv - Biochemistry 2023Quote: ... SsoPox variants were used at 1 mg.mL-1 in Tris buffer (50 mM Tris-HCl, pH 7) and with 1X SYPRO® orange (Sigma-Aldrich). Denaturation was followed using FRET channel ...
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bioRxiv - Neuroscience 2023Quote: We used the odorants methyl hexanoate (MHXE, CAS: 106-70-7) and 2-heptanone (HEPN, CAS: 110-43-0) (Sigma Aldrich). Odorant stimuli were generated with a custom-made stimulator optimized for temporal precision in binary mixtures [13] ...
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bioRxiv - Neuroscience 2023Quote: Fly brains from L3 larvae or 5–7-day old adult flies were dissected in ice-cold insect media (Schneiders media) (Sigma Aldrich) and fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2023Quote: ... mice and rats (male Sprague-Dawley, aged 7-10 weeks) were subcutaneously injected with the VEGF-receptor inhibitor SU5416 (Sigma-Aldrich) at a dosage of 20 mg/kg body weight under isoflurane anesthesia ...
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bioRxiv - Cell Biology 2023Quote: ... cells were split into 2 x 4 ml cultures and either fixed straight away with 3.7% formaldehyde (final concentration) or subjected to treatment (200 ng/ml rapamycin or 0.5 mg/ml calcofluor white (Sigma-Aldrich 910090)) for 1 hour ...
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Cell Biology 2023Quote: LPS- and OGD-treated cells were washed with PBS and stained with 5 μM 2′,7′-dichlorofluorescin diacetate (DCFH-DA; Sigma-Aldrich) for 20 min at 37°C to detect cell reactive oxygen species (ROS) ...
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bioRxiv - Neuroscience 2023Quote: ... seven tree shrews (TS 1–TS 7) were used to determine the appropriate dose of MPP+ (MPP+ iodide, D048, Sigma, USA) required for PD model development ...