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Citations for Millipore Sigma :
2251 - 2300 of 4180 citations for 6 Propylamino 7 deazapurine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and 100 μl spread on either LB agar plates with or without specified antibiotics (2 μg/ml amikacin disulfate salt or 7 μg/ml nitrofurantoin (both Sigma-Aldrich) or 50% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... bone marrow cells were flushed from dissected femurs and tibias and cultured for 7 days in macrophage growth medium (RPMI with 10% heat-inactivated FBS (Sigma-Aldrich), 10% 3T3 m-CSF ...
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bioRxiv - Developmental Biology 2022Quote: ... (1 mg/ml) and incubated in 50 μl droplets of 10 µM 2′,7′- dichlorodihydro-fluorescein diacetate (H2DCFDA, D6883, Sigma-Aldrich) in PVA-PBS for 15 min at 37 °C in an atmosphere of 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... The retinal strips were sectioned (7–10 µm thick) and placed on microscope slides that were treated with 3-Aminopropyltriethoxysilane (APES, Sigma A3648). Before immunohistochemistry ...
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bioRxiv - Plant Biology 2021Quote: ... Lipids extracts were incubated 1 h at 50°C in 2 mL of methylamine solution (7 ml methylamine 33% (w/v) in EtOH combined with 3 mL of methylamine 40% (w/v) in water (Sigma Aldrich) in order to hydrolyze phospholipids ...
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bioRxiv - Plant Biology 2021Quote: The glass fibre filter papers (GFFPs) used to collect each sample were submerged in 7-mL volumes of HPLC-grade methanol (>99.9%, Millipore-Sigma Canada) in 20-mL scintillation vials (Chromatographic Specialties Inc. ...
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bioRxiv - Microbiology 2020Quote: ... CD14+ macrophages differentiated for 7 days in M-CSF (described above) were detached from the petri dish using 2 mM ethylenediaminetetraacetic acid (EDTA, Sigma Aldrich) in PBS at 4 °C as well as mechanical scraping ...
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bioRxiv - Cancer Biology 2020Quote: ... and the pellet resuspended and incubated for 3-7 minutes at 37°C in accutase dissociation reagent (Sigma, Poole, UK, A6964). After enzyme neutralization with fresh medium ...
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bioRxiv - Neuroscience 2022Quote: ... Three to five weeks after reprogramming, single colonies were picked and expanded on SNL76/7 feeder cells (ATCC, SCRC-1049) treated with mitomycin C (Sigma Aldrich) in 20% KSR medium [92] ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then separated by 10% SDS-PAGE in a Bio-Rad mini-gel system (7×8.4cm) and transferred onto polyvinylidene fluoride membrane (EMD Millipore, IPFL00010). The membrane was blocked with TBS-T (20 mM Tris-HCl ...
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bioRxiv - Systems Biology 2022Quote: ... each lane was partitioned into three separate MW fractions and equilibrated in 100mM ammonium bicarbonate (Millipore SIGMA; CAS # 1066-33-7). Each gel plug was then digested overnight with Trypsin Gold ...
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bioRxiv - Neuroscience 2019Quote: ... The arenas were covered with a watch glass (7 cm diameter, 8 mm height in the center) which was coated on the inner side with Sigmacote (Sigma-Aldrich) to prevent flies from walking on the inside of the glass ...
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bioRxiv - Developmental Biology 2019Quote: ... H9 hESCs were nucleofected using the Lonza P3 Primary Cell 4D-Nucleofector X Kit and monoclonal colonies were selected for 7-10 days with 1 μg/ml of puromycin (Sigma-Aldrich). Tetracycline hydrochloride (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... 100 nM ALK2/3 inhibitor LDN193189 (Miltenyi Biotech, 130-106-540) and 10 uM ALK4/5/7 inhibitor SB431542 (Millipore, 616464). Medium was replaced on days 1 and 2.
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bioRxiv - Plant Biology 2019Quote: ... wild and transgenic seeds were grown on 0.8% agar-solidified 1/2 MS vertically oriented plates for 7 days with or without specific concentration of Propyzamide (Sigma-Aldrich). To detect the morphology of the cells of 7-day-old seedlings ...
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bioRxiv - Immunology 2020Quote: ... A piece of lung was dissected in ∼0.5 cm cubes and resuspended in 7 ml serum free RPMI containing 30 μg/ml DNAse and 700 μg/ml collagenase (Sigma Aldrich) and in C Tubes (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2020Quote: Accumulation of ROS was assayed by confocal microscopy of seedlings stained with the fluorescent dye 2’,7’-dichlorofluorescin diacetate (H2DCFDA; Sigma-Aldrich) as described previously (Achard et al. ...
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bioRxiv - Bioengineering 2019Quote: ... SKBR3 cells were starved for 24 h in serum-free medium and then stimulated for 15 min with 7-15 nM HRG-β1 (Sigma Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... The resultant pellets were resuspended in 100 μl of 1X PBS then incubated with 7 μl of 4% (w/v) phosphotungstic acid (Sigma-Aldrich) in 0.2 M magnesium chloride ...
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bioRxiv - Microbiology 2021Quote: ... The remaining mosquitoes were maintained under optimal conditions in the insectary and their midgut dissected on day 7 PBM to determine prevalence and load of infection by counting oocysts on mercurochrome (0.4% w/v- Sigma-Aldrich-M7011) stained midguts using a light microscope (X20 & X40).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... University of Nottingham) and Cos-7 (ATCC) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich or Thermo Fisher Scientific) supplemented with 10 % fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2019Quote: ... we repeated our measurements after 30 min superfusion in 10−7 M TTX and 10−5 M picrotoxin (PTX; Sigma-Aldrich). PTX blocks glutamatergic synapses in the STG of both C ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by SDS-PAGE in a BioRad mini gel system (7 × 8.4 cm) and transferred onto polyvinylidene fluoride membrane (EMD Millipore, IPFL00010). The membrane was blocked with TBS-T (20mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: Human leukaemia cells HL-60 and breast cancer cell line MCF-7 were cultured in RPMI and DMEM media (Sigma-Aldrich), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... and 500 μL) were added to each well along with a no virus control with 7 μg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... 2003): Notch1-CreERT2/R26mTmG mice of 5-7 months of age received a single intraperitoneal injection of Azoxymethane (AOM, Sigma #A5486) followed by Dextran Sulfate Sodium (DSS ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated as described in each legend respectively (MG132 – Sigma M7449; 5Z-7-oxozeaenol – Sigma O9890; TAK-715 – MedChem Express HY-10456; nocodazole – Sigma M1404). Equal volume DMSO was applied to cells as a vehicle control for compounds dissolved in DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were introduced in RPE-1 cells using Fugene HD transfection reagent and cells were selected based on GFP-expression or using 7 μg/mL puromycin (Sigma Aldrich) for 5 days.
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bioRxiv - Microbiology 2020Quote: ... parasites were maintained below ~7×105 cells per ml for up to 5 days prior to addition of brain heart infusion (BHI) broth (Sigma Aldrich).
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bioRxiv - Microbiology 2019Quote: Interactions between the SRB1 and NS1 proteins from DENV and ZIKV in Huh-7 and C6/36 cells were detected using the commercial kit Duolink (Sigma-Aldrich) based on in situ proximity ligation assay (PLA ...
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bioRxiv - Immunology 2021Quote: ... CD14+ monocytes were differentiated to MDMs by culturing for 7 days (at a concentration of 1 × 106 cells ml−1) in RPMI 1640 (Sigma, R8758) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: Lentiviral particles encoding guide RNA and Cas9 were produced in HEK 293T cells using mouse Irf7 CRISPR lentiviral transfer construct targeting exon 7 (target ID #MM0000202587) of Irf7 (Sigma Aldrich). Lenti CRISPR universal non-target plasmid was used as a control (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... that contain two parallel channels separated by a porous membrane (7 μm pores) were first perfused and incubated with 1% 3-Aminopropyltrimethoxysilane (Sigma, 281778) in ethanol for 1 hour and then incubated in an 80°C oven overnight ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Physiology 2020Quote: α-synuclein aggregates in the head muscles of 7-days old worms were visualized by fluorescence microscopy (400x magnification) in worms anesthetized with 15 mM sodium azide (Sigma, S2002). Pictures were segmented using Ilastik (version 1.3.0 ...
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bioRxiv - Biophysics 2022Quote: ... to which a 5 % of fluoro-surfactant was added (1H,1H,2H,2H-Perfluoro-1-octanol, CAS Nr 647-42-7, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... 7×103 cells were resuspended in 100μL of appropriate cell-culture media containing 1μg/mL hydrocortisone (Sigma, H0888, lot no. SLBG4963V) and 5μg/mL insulin (Sigma ...
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bioRxiv - Genomics 2022Quote: ... head kidney and spleen of 30 challenge fish were collected at three timepoint (pre-infection, 7 dpi and 14 dpi) into TRI Reagent (Sigma, UK) and stored at −80°C until RNA extraction.
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bioRxiv - Microbiology 2022Quote: ... 100-200 μl culture aliquots were transferred to 2 ml Eppendorf tubes with perforated lids followed by addition of 7 μM (10 μg/ml) polymyxin B (Sigma-Aldrich) or 31 μM (30 μg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... hCAI with the K978C mutation (h^K978C) or for T2A mutagenesis were designed using SnapGene software (SI, Table 7) and synthesised by Sigma Aldrich.
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bioRxiv - Microbiology 2024Quote: ... Primary human PBMC were differentiated into macrophages (hMDMs) after culturing for 7 days with RPMI supplemented with 20% heat-inactivated human serum (Sigma-H4522).
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bioRxiv - Synthetic Biology 2024Quote: ... 50 nM readout probes in 10% EC Buffer (1 mL of ethylene carbonate, 7 mL Ultrapure RNase/DNase free water, 2 mL 20x SSC, and 1g Low MW dextran sulfate Sigma #D4911) was added to the cells and incubated for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... containing a sgRNA sequence (5’-TCATCGCTCACAACCAAGTG-3’) targeting exon 7 of ERBB2 (cloned by Twin Helix) and selected with puromycin (Sigma-Aldrich) for 4 days (1 μg/ml puromycin for 72 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 7 x 105 cells/well were plated onto each well of 10 µg/mL Poly-D-lysine (PDL, Sigma-Aldrich, P6407-5MG ...
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bioRxiv - Neuroscience 2024Quote: ... Otr-Cre male mice (aged 5–7 weeks) were anesthetized using a combination of 65 mg/kg ketamine (Daiichi-Sankyo) and 13 mg/kg xylazine (Sigma-Aldrich) via intraperitoneal injection ...
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bioRxiv - Neuroscience 2024Quote: ... Cre-mediated recombination was induced in mice 3-7 months of age by intraperitoneal injection of 2 mg/day tamoxifen (Sigma-Aldrich) diluted with 300 μl corn oil (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... In-cell SHAPE modifications were made by adding fresh 500µl of 100mM 1-Methyl-7-nitroisatoic anhydride (1M7) (Sigma-Aldrich, 908401) in DMSO to 4.5ml pre-warmed culture media to the dish and incubated for 3min at 37C ...
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bioRxiv - Cell Biology 2024Quote: ... were carefully dissected 7 to 16 days after induction of GAL4 expression at 29°C in Shield and Sang M3 medium (Sigma S3652), keeping the crop ...
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bioRxiv - Cancer Biology 2024Quote: ... Two sets of four EV-rich fractions (7–12 and 16-22) were pooled and concentrated using Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore, USA). Alternatively ...
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bioRxiv - Cancer Biology 2023Quote: ... Myelin and debris removal was performed by a gradient centrifugation of the cell suspension in a 7:3 mix of PBS:Percoll (Sigma-Aldrich, #P4937), with an additional PBS wash ...