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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Animals carrying the chil-27p::GFP reporter (icbIs5) were mutagenised using the chemical mutagen ethyl methanesulfonate (Sigma). L4 stage animals (P0 ...
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bioRxiv - Plant Biology 2022Quote: ... Seeds were soaked in distilled water overnight and then treated with 0.2% EMS (ethyl methanesulfonate, Sigma Aldrich) for 6 hours ...
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bioRxiv - Neuroscience 2022Quote: ... flp-13pr::ACR-12::GFP strain IZ1905 animals were treated with 25 µM ethyl methanesulfonate (EMS, Sigma). After washing ...
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bioRxiv - Immunology 2022Quote: ... col-12p::mCherry] transgene were treated with 24 mM ethyl methanesulfonate (EMS) (Sigma-Aldrich, St. Louis, MO) using previously described procedures [54] ...
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bioRxiv - Neuroscience 2024Quote: ... Lysate volumes were made up to 250μl Tri-ethyl ammonium bicarbonate buffer (100mM) (TEAB buffer, Sigma, #T7408) followed by gentle mixing ...
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bioRxiv - Cell Biology 2020Quote: ... washed 3 × 5 mins in phosphate-buffered saline (PBS) with 0.1% Tween-20 (both Sigma-Aldrich) and probed for 1 hour at room temperature in the dark with IRDye® 800CW conjugated goat anti-mouse secondary antibodies (LiCOR Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... shLmnA (produced in our lab), shLMNC (produced in our lab, 5′-TCTCCCACCTCCATGCCAAAG-3’) or shLMNB1 (Sigma clone NM_010721.1-956s1c1 ...
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bioRxiv - Physiology 2020Quote: ... and T3 (3,3’,5-triiodo-L-thyronine, > 95% HPCL, CAS number 6893-02-3, Sigma-Aldrich) were first dissolved in 0.1M NaOH and then diluted in 0.9% NaCl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3-5 μL of biliverdin hydrochloride (chromophore for iRFP signal activation, final concentration 0.002%, Sigma; 30891) added to cells and incubated for 10 mins at 37 °C before imaging ...
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bioRxiv - Microbiology 2020Quote: ... 1 mg ml-1 of 3–5 kDa fluorescein isothio-cyanate (FITC)-dextran (Sigma-Aldrich, Germany) in phenol-red free DMEM/F12 medium (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Then washed for 5 min x 3 times and blocked in 1% BSA (A3059, Sigma-Aldrich) (w/v ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were washed 3×5 minutes in TBST buffer and incubated with ChAT antibody (Sigma, AB144) at 1:100 dilutions overnight at 4°C in the blocking buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were allowed to differentiate for 5 days with differentiating media [3 μg/ml prolactin (Sigma, #L6520 ...
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bioRxiv - Cell Biology 2022Quote: ... The reagents treated for some indicated experiments include: 3-5 µM oligomycin A (Sigma, Cat# 75351), 1 µM (for HeLa cells ...
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bioRxiv - Neuroscience 2021Quote: ... 300 μM of the PKA activator 8-Bromoadenosine 3’,5’-cyclic monophosphate (Sigma, dissolved in water) and without FBS ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-AGTTTCTCTGTCCGATTTAAA-3’] are in the plasmid backbone pLKO.1-CMV-tGFP and were from Sigma Millipore (St Louis ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with PBS 3 times and blocked with 5% normal donkey serum (Sigma-Aldrich) in wash buffer for 1 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein 5-HT2C or HCA2 (3 μg) was immobilized on nickel agarose beads (Sigma) in the incubation buffer containing 50 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... and guanosine-5’-γ-3-thiotriphosphate (GTPγS) were acquired from Sigma-Aldrich (St. Louis, MO, USA). WIN 55,212-2 was purchased from Tocris Bioscience (Bristol ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were infected with pLKO.1 control or pLKO.1-shZSCAN4 (5’-GAATGCAACAACTCTTGTAATCTCGAGATTACAAGAGTTGTTGCATTCT-3’, Millipore Sigma) and further selected with Puromycin (1ug/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and Mannitol (3 g of Bacto Peptone [Becton Dickinson], 5 g of Yeast Extract [Sigma-Aldrich] ...
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bioRxiv - Bioengineering 2023Quote: ... All reaction mixtures contained 250 nM of sirtuin (SIRT1-3, 5 Reaction Biology, SIRT4,6,7 Sigma-Aldrich), 10 µM furimazine (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were maintained at <3% parasitemia and routinely synchronized by treating with 5% sorbitol solution (Sigma) for ring stage and by using percoll gradient (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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bioRxiv - Systems Biology 2024Quote: ... 8-Br cAMP (8-Bromoadenosine 3′,5′-cyclic monophosphate, final conc. 500 µM; Sigma B6386-100mg). In addition to stimulation for decidualization ...
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bioRxiv - Physiology 2024Quote: ... Samples were washed (3 times, 5 min each) with PBS containing 0.1% Triton X100 (Sigma-Aldrich), and then incubated with secondary antibody (Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were subsequently washed 3 x 5 minutes in PBST 0.01% (DPBS ThermoFisher, Tween P1379 Sigma), permeabilised for 15 minutes in PBST 0.5% and washed in PBST 0.01% a further three times ...
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bioRxiv - Physiology 2024Quote: ... tissues were washed (3 x 5 min) in PBS containing 0.1% Triton X-100 (Sigma-Aldrich) and incubated with mouse anti-TARDBP primary antibody or mouse anti-HuC/D (for total neurons ...
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bioRxiv - Bioengineering 2024Quote: ... KG) followed by incubation in freshly made 5% (3-Aminopropyl) triethoxysilane (Sigma-Aldrich, Cat. no. 440140) in deionized water for 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The control siRNA is a scrambled sequence (5’-UUCUCCGAACGUGUCACGUdTdT-3’) (NC, Sigma, St. Louis, MO, USA). A total of 60 nM siRNAs was used for transfection using Lipofectamine-RNAi-MAX Reagent (13778 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde and the slide was mounted using a medium containing 4′,6-diamidino-2-phenylindole-DAPI (Fluoroshield, Sigma-Aldrich, USA). A fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 h at 4°C before being rotated overnight at 4°C with Anti-acetyl-Histone H3 (Lys27) (Sigma; 07-360) or Anti-MAFG (GeneTex ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two days at 4°C with secondary antibody along with 0.01 mg/ml 4′-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, Cat#: D9542) to label cell nuclei fluorescently and either 0.2 µl/ml phalloidin-iFluor 594 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... The samples were fixed overnight at 4°C in the mixture of 4% paraformaldehyde (Cat. No. 162-16065, Wako Pure Chemical Industries) and 2% glutaraldehyde (Sigma-Aldrich, G5882) in PBS ...
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bioRxiv - Genomics 2024Quote: ... slides were counterstained in 4’,6-diamidino-2-phenylindole (DAPI) solution and embedded in Mowiol 4–88 mounting medium (Sigma-Aldrich, USA).
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bioRxiv - Cell Biology 2020Quote: ... 150,000 cells were stained with 10 μM 4’-(trimethylammonio)-diphenylhexatriene (TMA-DPH) or 2 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine; both obtained from Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... diluted 1:500 in 1% BSA with 0.3% Triton X-100 for 1.5 h in RT and stained with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, #D9542) for 10 min in RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Mice were kept on 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. No. PHR1731-1G) in drinking water when indicated ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed with PBST and incubated at room temperature for 2 h with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) diluted in PBST ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... for 15 min and blocked for 2 hours at 25 °C or overnight at 4 °C using 2% BSA (Sigma-Aldrich). Samples were incubated with respective primary antibodies at 4 °C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were placed in a cylindrical phantom (2 cm diameter, 2 % agarose mixed with 5 % intralipid (Sigma-Aldrich AG, Switzerland). The phantom holder was placed in an imaging chamber filled with water and kept at 28°C.
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bioRxiv - Neuroscience 2023Quote: The mice received three injections every 2 h (200 ul/injection, intraperitoneally in alternative sites of the body) of 5-Chloro-2’-deoxy-Uridine (CldU, Sigma reference C6891 ...
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bioRxiv - Immunology 2024Quote: ... NK cells were pretreated with DMSO or 2-DG (0.5 mM, 1 mM, 2 mM, 5 mM; Sigma-Aldrich, USA), BAY 87 (10 μM ...
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bioRxiv - Neuroscience 2024Quote: ... 5-Ethynyl-2′-deoxyuridine (EdU) injections were administered 2 weeks after BrdU using the same injection protocol (Sigma Aldrich, #900584).
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bioRxiv - Neuroscience 2024Quote: ... one hemisphere of each mouse brain without cerebellum and olfactory bulbs was placed in fluorescence-activated cell sorting (FACS) buffer (1x PBS, 2% FCS and 2 mM EDTA) with 5 μM actinomycinD (ActD; Sigma) and processed for scRNA sequencing as previously described48 ...
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bioRxiv - Neuroscience 2021Quote: A solution containing 10 μM of 5-bromo-2’-deoxyuridine (BrdU; SIGMA #858811) was added to the growth medium of SK-N-SH cell monolayers and cells were incubated at 37°C for 2 hours to estimate the S-phase index ...
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bioRxiv - Biophysics 2020Quote: ... 10 mM NaN3 and 5 mM 2-deoxy-D-glucose (Sigma-Aldrich, Singapore).
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...