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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The sections were stained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, D9542) mounted on the objective slides ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; D9542, Sigma-Aldrich) for 5 min at 1:1000.
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bioRxiv - Immunology 2024Quote: ... Cell nuclei were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich). Another randomly chosen series was used for Nissl staining in order to determine the total volume of the granular cell layer (GCL ...
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bioRxiv - Immunology 2024Quote: ... additional staining with 4’,6-diamidino-2-phenylindole (DAPI, 100 ng/ml; Sigma-Aldrich) was performed ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by a 2-minute perfusion with 4% paraformaldehyde (PFA; Sigma-Aldrich) in PBS (7.4 pH ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by 4’,6-diamidino-2-phenylindole (DAPI) (Sigma, 200 ng/ml) staining for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... final concentration of 2 µM carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP, #C2920, Sigma-Aldrich), 1 µM oligomycin (#75351 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES buffer) were purchased from Sigma Aldrich. 20 mg/mL of protein was mixed in 50 mM HEPES at pH 7.1.2 M ammonium sulphate in 0.1 M Tris-HCl at pH 8 was used as the precipitant solution.41 Crystallization trials were set up similar to lysozyme.
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bioRxiv - Immunology 2024Quote: ... Adherent cell lines were split every 2-4 days using Trypsin/EDTA (Sigma Aldrich). Media was renewed for all cultures and experiments every 48 hours.
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bioRxiv - Cell Biology 2024Quote: ... 25mM NaCl in PBS) with DAPI (4’,6-diamidino-2-phenylindole) (D9542, Sigma-Aldrich) for 5-7 days ...
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bioRxiv - Genomics 2024Quote: ... and nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, #D8417). The fluorescent images were obtained with a Zeiss Axio Observer inverted microscope (40x NA 1.3 oil objective ...
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bioRxiv - Biophysics 2024Quote: ... The cells were then counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) to exclude dead cells during analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma, 32670-5MG-F) and mounted using antifade mounting media.
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were counterstained with 0.5μg/ml 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and coverslips were mounted using Vectashield (Vector Laboratories) ...
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bioRxiv - Physiology 2021Quote: ... were incubated at 4 °C overnight with 5 μg/mL anti-FLAG (Sigma-Aldrich, F1804) in 200 mM NaHCO3 buffer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... consisting of 4% (v/v) of 19:1 acrylamide/bis-acrylamide (Sigma-Aldrich, A9926-5), 60 mM Tris·HCl pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... 4-morpholineethanesulfonic acid hemisodium salt (5 ml, 0.5 M in MQ water; #M0164, Sigma-Aldrich) was added ...
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bioRxiv - Developmental Biology 2021Quote: ... were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449, Millipore), 5ug H2AK119ub (8240 ...
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bioRxiv - Cancer Biology 2020Quote: ... an uncoupling agent carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) (5 μM) (Sigma Aldrich, Missouri, USA). and antimycin-A (2 μM ...
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bioRxiv - Developmental Biology 2021Quote: ... Larvae older than 4 dpf were first soaked in 5 mg/ml epinephrine (Sigma-Adrich) for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and the bands were developed using disodium 3-[4-methoxyspiro [1,2-dioxetane-3,2’-(5’-chloro)tricyclo (3.3.1.13,7) decan]-4-yl]phenyl phosphate (CSPD; Sigma-Aldrich} ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 4-5 days with 0.5 mM EDTA in DMEM/F12 (Sigma). For introduction of TRE3G-NGN2 into the AAVS1 site ...
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bioRxiv - Bioengineering 2023Quote: ... fibroblast cells (4 ·104 cells/ml) and collagen I (5 ng/µl, Sigma-Aldrich, USA) was added to the wells of a 96-well plate ...
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bioRxiv - Immunology 2023Quote: ... and IL-5 cultured cells were pulsed (4 hr) with BrdU (10 µM) (Sigma-Aldrich) prior to flow staining with an anti-BrdU-Alexa fluor 647-conjugated antibody (BD Pharminogen ...
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bioRxiv - Systems Biology 2024Quote: ... each with 4 thoraces were homogenized in 100 µl of 5 M guanidine-HCl (Sigma) supplemented with 100 mM Tris pH 8.0 and 5 mM EDTA pH 7.8 ...
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bioRxiv - Systems Biology 2024Quote: ... 5 uL of 10 mg/mL of proteinase K (Millipore Sigma, Cat. No. 70663-4) was added and samples were incubated for 1 hour at 55°C ...
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bioRxiv - Bioengineering 2024Quote: ... fibroblast cells (4 104 cells/ml) and collagen I (5 ng/µl, Sigma-Aldrich, USA) was added to the wells of a 96-well plate ...
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bioRxiv - Neuroscience 2021Quote: ... cells were subcultured onto glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... Tadpoles were exposed to 4’-OH-PPF ± 5 nM T3 (3,3′,5-triiodo-L-thyronine sodium salt (T3) (Sigma - CAS 55-06-1)).
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bioRxiv - Neuroscience 2024Quote: ... cells were subcultured on glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed again in washing buffer (2 × 5 min) before being incubated in the same buffer with 5% bovine serum albumin (BSA, Sigma, A7030) as blocking solution for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were cultured at 37°C/5% CO2 and glial growth was inhibited at DIV4 by addition of 5-fluoro-2’-deoxyuridine (30 μM)(Sigma, F0503). A 50% fresh culture medium change was performed every 4 days.
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bioRxiv - Cancer Biology 2021Quote: ... specific siRNAs (si-HER3-1: 5’-GCUGAGAACCAAUACCAGA, si-HER3-2: 5’-CCAAGGGCCCAAUCUACAA) and a validated control siRNA were obtained from Sigma-Aldrich.
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bioRxiv - Microbiology 2021Quote: ... with 1.5% agar supplemented with 2 mg/mL 5-Fluoroorotic acid (US Biological, USA) and 5 µg/mL uracil (Sigma-Aldrich USA). Confirmation of plasmid excision was made by negative selection in BHIS-15% thiamphenicol plates ...
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bioRxiv - Developmental Biology 2023Quote: RNA extraction of 7 dpf larvae (5 dpf +2 days of drug treatment) in batches of 5 individuals was performed using TriZol reagent (Sigma-Aldrich) and RNA purification kit (Zymo) ...
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bioRxiv - Biochemistry 2023Quote: ... Labeling was carried out at room temperature for 2 hours with end-to-end rotation and quenched with 5 µL of 5% hydroxylamine (Sigma Aldrich) for 15 minutes ...
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bioRxiv - Zoology 2020Quote: ... 3-methyl-but-2-enyl acetate and (2E)-hex-2-enyl acetate were confirmed with analytical standards from Sigma-Aldrich (St. Louis, MO). For the analysis of CHCs ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid 3-azido-1-propanol ester chain transfer agent (CTA-azide) were purchased from Sigma-Aldrich (Macquarie Park, Australia). DBCO-modified Ang-2 peptide (TFFYGGSRGKRNNFKTEEYC(Mal-DBCO) ...
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bioRxiv - Microbiology 2022Quote: The AHL signaling molecules used in this study are N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL, Sigma-Aldrich CAS# 168982-69-2), N-butyryl-homoserine lactone (C4-HSL ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were trimmed down to a 3-5 mm region around the area of interest and embedded in 5% (w/v) agarose (A9539; Sigma-Aldrich, St. Louis, MO, USA) in deionized water ...
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bioRxiv - Neuroscience 2023Quote: ... minced using sterile razor blades and digested in a 5 mL tube containing 3-5 mL of the digestion mix at 37 °C for 90 minutes: Collagenase type V (Sigma-Aldrich C9263; 5 mg/mL) and Dispase II (Gibco 17105041 ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected brains were post-fixed for 2 h at 4°C in 4% PFA and cryoprotected in successive solutions of 15% and 30% sucrose (Sigma-Aldrich, Cat# S0389) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: Vital dye staining of lateral line hair cells of the 4 dpf embryos was performed using DASPEI (2-(4-(dimethylamino) styryl)-N-ethylpyridinium iodide) (Sigma-Aldrich, USA, D0815), 0.8 µg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was diluted 1/10 with McIlvaine citrate-phosphate buffer pH 4.5 and activity assayed with 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyranoside (Sigma, Dorset, UK) in a plate reader.
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bioRxiv - Cancer Biology 2024Quote: ... medium supplemented with 10 mM of 2-(4-(2-hydroxyethyl) piperazin-1-yl) ethane sulfonic acid (HEPES; Sigma-Aldrich, St. Louis, MO), 24 mM sodium bicarbonate ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies at RT for 2 hours with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542). Afterward ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed with PBS and incubated with 100 µM 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2NBDG) (Sigma Cat# 186689-07-6) containing RPMI1640 (HiMedia Cat# AT150 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice with PBS1X (5 mM EDTA, 2% fetal bovine serum (FBS)) before being fixed in 2% formaldehyde solution (252549, Sigma-Aldrich). After incubation at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
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bioRxiv - Microbiology 2024Quote: ... 2 ug mL-1 1-[(5S)-4,5-Dihydro-5-phenyl-1H-pyrazol-1-yl]-2 (GSK’963) (Sigma-Aldrich # SLM2376- 25MG), 2 ug mL-1 N-(6-(isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine hydrochloride (GSK’872 ...