Labshake search
Citations for Millipore Sigma :
2151 - 2200 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted in blocking solution and added to samples for 1 hour at room temperature (RT): ACTN1 (Sigma, A7811), TAGLN (Abcam ...
-
bioRxiv - Immunology 2022Quote: ... Real time RT-qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma). For tissue conditioned media (TCM ...
-
bioRxiv - Immunology 2022Quote: ... Real time RT-qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma).
-
bioRxiv - Genomics 2020Quote: ... Subsequently sections were incubated for 1h at RT in PBS blocking buffer containing 5% horse serum (Sigma Aldrich, Cat. N: H0146), 0.3% Triton X-100 (Sigma Aldrich ...
-
bioRxiv - Genomics 2020Quote: ... in situ cDNA synthesis reaction was performed by the addition of 75μl RT reagents: 50ng/μl actinomycin D (Sigma-Aldrich, USA), 0.5mM dNTPs (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2019Quote: ... Ipsilateral trigeminal ganglia were dissected and both skin and trigeminal ganglia were homogenized for three minutes (skin) or one minute (TG) in 1 mL RNAzol RT (Sigma-Aldrich). Thoracic spinal cord was dissected from mice treated with 40 µL MC903 or ethanol on the shaved rostral back skin and homogenized for one minute in 1 mL RNAzol ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were washed twice with PBS and cross-linked with a solution of 1 % formaldehyde in PBS (7.5 min at RT, shaking) prepared from a 37 % stock (Sigma-Aldrich, F1635). Cross-linking was neutralized by adding glycine for a final concentration of 0.125 M ...
-
bioRxiv - Neuroscience 2019Quote: ... Nitrocellulose membranes were washed 3 times for 10 min with TBST and incubated with HRP-conjugated secondary antibodies for 1 h at RT (1:1000; Sigma-Aldrich, St ...
-
bioRxiv - Cell Biology 2019Quote: ... incubated with 1.45 µL of PC-Biotin-NHS (50mM) (Ambergen) for 40 minutes at RT with gentle rotation and 400 µL of standard Streptavidin-Agarose bead slurry (Sigma Aldrich). Over the course of the project ...
-
bioRxiv - Neuroscience 2021Quote: Animals were anesthetized and perfused through the left ventricle with 0,2% Heparin in PBS at RT followed by ice-cold 10% formalin solution (Sigma HT501128-4L). The brains were dissected and post-fixed for 72 h in fresh 4% PFA (Thermo Fisher Scientific #28906 ...
-
bioRxiv - Neuroscience 2020Quote: Free-floating sections were blocked for 60 min at room temperature (RT) in PBS containing 10% donkey normal serum (catalog: D9663, Sigma-Aldrich), 5% bovine serum albumin (catalog ...
-
bioRxiv - Developmental Biology 2021Quote: ... They were blocked in 1% BSA in PBT for 1hr at RT and incubated overnight at 4°C with the monoclonal mouse anti-FLAG M2 antibody (Sigma, F1804) diluted 1:500 in 1% BSA/PBS ...
-
The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hr in blocking solution (PBS with 0.5% Triton X-100 and 5% goat serum) at RT followed by rat monoclonal anti-SST primary antibody (1:100; MAB354, Sigma-Aldrich) for at least 12 hr at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... blocked and permeabilized for 1 h in 10% donkey serum/PBS-T/0.3% triton-X-100 pH 7.4 and incubated for 2 h (RT) in mouse anti-puromycin 12D10 antibody (Sigma Aldrich, MABE343), washed extensively ...
-
bioRxiv - Microbiology 2020Quote: ... Nuclei were labelled with a PBS-Hoescht solution at 2 μg/mL for 5 min at RT and samples were finally mounted in mounting medium (F4680, Sigma Aldrich). Labelled cells and EVs were analysed with an inverted LSM 880 confocal microscope with airyscan (Carl Zeiss Ltd.) ...
-
bioRxiv - Systems Biology 2020Quote: ... diluted 1:500 in 1% BSA with 0.3% Triton X-100 for 1.5 h in RT and stained with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, #D9542) for 10 min in RT ...
-
bioRxiv - Microbiology 2021Quote: ... the cultures were harvested by centrifugation (20,000 × g for 3 min at RT) and the pellet was resuspended in a volume of 2× Laemmli buffer (Sigma-Aldrich) that adjusts for the total number of bacteria according to the OD600 of the initial cultures followed by 15 min incubation at 95 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... for 1hour at RT and then incubated overnight at 4°C with a mouse anti-βIII-Tubulin (TUJ1, Sigma, 1/500) and a rabbit anti-ISLET1 (Abcam ...
-
bioRxiv - Cell Biology 2021Quote: ... diluted in water for 20 min at RT followed by washing with PBS and fixation with 0.5 % (v/v) glutaraldehyde (Sigma-Aldrich, USA) for 15 min on ice ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
-
bioRxiv - Neuroscience 2022Quote: ... 4 times for 10 min at RT and then incubated with the secondary antibodies directed against goat IgG coupled to horseradish peroxidase (1:100,000 – Sigma, Munich, Germany) dissolved in the blocking solution ...
-
bioRxiv - Pathology 2022Quote: ... 1 part NTA), incubated (30min., RT) and centrifuged (3000g, 1h, 4°C) in an Ultra-2 Centrifugal Filter Unite (Millipore, #UFC203024). Filtered samples (100µL ...
-
bioRxiv - Neuroscience 2022Quote: ... At the end of the incubation period (1h at RT) bound and free ligands were separated by rapid filtration over Whatman brand Grade GF/C glass filters (Sigma-Aldrich) using a sampling vacuum manifold (MilliporeSigma) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Slides were incubated in a humidified chamber at RT for 1h with blocking solution (5% normal donkey serum (Sigma, Cat#D9663) in PBS with 0.1% Tween 20) ...
-
bioRxiv - Cancer Biology 2022Quote: ... discs were dissected and fixed as described above and incubated 1 hour at RT with Rhodamine-conjugated Phalloidin (Sigma, P-1951) at 0,3 mM in phosphate buffer 0.2 % Triton supplemented with 10 % horse serum ...
-
bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature (RT) and revealed by chemiluminescence using Immobilon Crescendo or Forte Western HRP substrate (Millipore Merck).
-
bioRxiv - Cell Biology 2022Quote: ... fixed with 4% formaldehyde (SAV Liquid Production) for 10 min at RT and permeabilized with 0.1% Triton™ X-100 (Sigma Aldrich) in PBS for 10 min at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Supernatant was mixed with detection reagent in a 1:1 ratio and incubated at RT for 20 minutes in Greiner white 96 well microplates (Sigma Aldrich). Luminescence was measured in triplicate on a GloMax plate reader ...
-
bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were incubated for 2 h at RT and DAPI (concentracioon) was included in last washed for 30 min.Cells were mounted on slides with DPX (Sigma Aldrich, 06522). AIS morphology was analyzed using ImageJ software.
-
bioRxiv - Neuroscience 2021Quote: ... in TBST for 1 h at room temperature (RT) and incubated with primary antibodies against β-actin (mouse, 1:50,000, Sigma, A5316) and Myosin Light Chain 2 (rabbit ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples were sonicated for two times ten secs at 50% amplitude on ice and incubated for 30 min at RT in lysis buffer consisting of 6 M urea (Sigma-Aldrich), 2 M thiourea (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at RT with a combination of two primary antibodies: a mouse monoclonal anti-PCNA (1:500; Sigma-Aldrich; catalogue number P8825 ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were then washed thrice with PBS and incubated for 1h RT with the appropriate secondary antibodies and Hoechst 33342 (1 µg/mL Sigma-Aldrich). Cells were washed thrice with PBS and membranes mounted in Prolong Gold antifade mounting medium (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
-
An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 1h at RT with Avidin-Biotin complex (Vector) and then with DAB/H2O2 mix (Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2020Quote: ... with/without anti-Flag antibody then incubated (1h at RT) with nanogold-coupled secondary antibody and finally fixed with 2.5% glutaraldehyde EM grade (Sigma Aldrich, #16210) for 1 h ...
-
bioRxiv - Neuroscience 2020Quote: ... The signal was amplified by induction with avidin-biotin complexes (Vectastain Elite ABC-HRP (Vector, PK-6100)) for 30 min at RT and visualized with 3,3′-diaminobenzidine (Sigma, D-5637). Additionally ...
-
bioRxiv - Microbiology 2021Quote: ... Cultures were incubated at 30 °C with shaking with sample collection at 1 hour via centrifugation and resuspension in 600 μl of ~60 °C RNAzol RT (Sigma-Aldrich). After lysing cells by pipetting and vortexing in RNAzol ...
-
bioRxiv - Bioengineering 2021Quote: ... The flask was then completely covered with aluminum foil and the solution was left to stir overnight (approximately 18 h) at RT when it was quenched with 18 mg hydroxylamine hydrochloride (Sigma-Aldrich). The solution was dialyzed with molecular weight cut-off membranes (SnakeSkin™ Dialysis Tubing ...
-
bioRxiv - Cell Biology 2019Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Sigma-Aldrich) for 5-30 min at RT ...
-
bioRxiv - Cell Biology 2019Quote: ... blocked with 10% FBS for 25 min at RT then incubated with 1:1000 TOMM20 primary antibody (Sigma-Aldrich, cat. HPA011562) diluted in 5% FBS for 1-h at 37°C ...
-
bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.5M were added to 250 ml of saline buffer] at room temperature (RT) and then with 4% paraformaldehyde (PFA; # 158127 Sigma-Aldrich) in PBS (Phosphate Buffered Saline ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell suspensions were centrifuged again (350g, 10 min, RT) and resuspended in freezing medium containing 10% DMSO (Sigma-Aldrich, Schnelldorf, Germany) in FCS (PAN-Biotech ...
-
bioRxiv - Biophysics 2020Quote: The structured PDMS stamp was dried with clean dry air and inked for 45 min at room temperature (RT) with a mixture of fibronectin solution from bovine plasma (FN) (Sigma Aldrich) and labeled (cy3.5 Invitrogen labeling kit ...
-
bioRxiv - Immunology 2021Quote: ... To remove red blood cell (RBC) contamination the cell suspension was incubated for 5 minutes at RT in RBC lysis buffer (Sigma-Aldrich). Finally ...
-
bioRxiv - Cancer Biology 2022Quote: ... and cells were then incubated for 90 minutes at RT with 0.1% Triton X-100 containing Alexa Fluor 647-conjugated anti-puromycin antibody (1:5000; Millipore, MABE343-AF647). Cells were washed thrice with PBST for 5 minutes each ...
-
bioRxiv - Cell Biology 2022Quote: ... centrifuged at 4,000 rpm for 10 min at RT and the supernatant was filtered through a 0.45 μm filter (EMD Millipore, Billerica, MA). The lentivirus-containing medium was snap frozen in 1 mL aliquots and stored at −80°C ...