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Citations for Millipore Sigma :
2001 - 2050 of 2558 citations for Recombinant Rat Cntfr His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... membranes were coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma) and fibronectin (5 µg/cm2 ...
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bioRxiv - Cell Biology 2022Quote: ... The rabbit-derived secondary antibody (anti-rat) conjugated with 10-nm colloidal gold (Sigma, lot #SLBZ8963), the goat-derived secondary antibody (anti-mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Collagen type I solution from rat tail (C3867-1VL) and collagenase (C9263) were purchased from Sigma–Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-rat PLA plus and minus probes were made using the PLA probemaker kit (Sigma Aldrich) with a Goat anti-rat IgG antibody (Jackson Immunoresearch ...
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured as described with Rat/Mouse Insulin ELISA kit (Millipore Sigma) (26).
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bioRxiv - Neuroscience 2021Quote: Mouse neuroblastoma x rat neuron hybrid ND7/23 cells were purchased from Sigma-Aldrich (ECACC 92090903). They were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... Rats were initially trained to lick for sodium saccharin solution (0.2% in distilled water; Sigma 47839) during 60 min sessions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Orphan GPCR expression was detected using rat anti-HA tag (clone 3F10) antibodies (Sigma-Aldrich; 11867423001) or rabbit anti-myc tag antibodies (GenScript ...
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bioRxiv - Biochemistry 2020Quote: ... The shRNA lentiviral plasmids (pLKO.1-puro) for human/mouse/rat frataxin were purchased from Sigma. The RefSeq used was NM-008044 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... A single dose (100µl in rats, 20µl in mice) of complete Freund’s adjuvant (CFA, SIGMA Aldrich) was injected subcutaneously into the plantar surface of one hind paw using a microliter syringe with a 27-gauge needle ...
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bioRxiv - Neuroscience 2021Quote: ... female rats were subcutaneously injected with 1 mg progesterone (5 mg/mL; Sigma, St. Louis, USA) in peanut oil (Apotekproduksjon ...
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bioRxiv - Cell Biology 2021Quote: ... substrates were coated with 10 μg/cm2 collagen type-I from rat tail (Sigma, Cat # C3867) overnight at 4°C.
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bioRxiv - Cell Biology 2020Quote: ... E18 rat striatal neurons were obtained from BraintBits LLC and cultured on poly-d-lysine (Sigma) coated coverslips for 1 week in Neurobasal media (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... The rabbit-derived second antibody (anti-rat) conjugated with 10-nm colloidal gold (Sigma, lot #SLBZ8963) is available as a commercial product.
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bioRxiv - Immunology 2022Quote: ... 24 well tissue culture plates were pretreated with 0.2mg/ml of collagen from rat tail (Sigma; 1mg/ml stock diluted in 0.1M acetic acid ...
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bioRxiv - Neuroscience 2022Quote: Rats received a single intra-articular injection of either 3 mg/50 µL MIA (Sigma, UK) or 50 µL 0.9% saline (control group ...
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bioRxiv - Neuroscience 2023Quote: ... rats were injected with 30 mg/kg fluorescein isothiocyantate (FITC)-dextran (500 kDa, Sigma catalog #FD500S) to label the cerebrovasculature ...
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bioRxiv - Neuroscience 2023Quote: ... Rats were pre-treated intraperitoneally (i.p.) with 20 mg/kg of desipramine hydrochloride (Sigma, Poole, UK) in order to preserve noradrenergic neurons ...
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bioRxiv - Plant Biology 2022Quote: ... Exposure to primary antibodies (rat - anti GFP 3H9-Proteintech or mouse – anti Biotin B7653-Sigma-Aldrich) was done for 2 hours at 25°C ...
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bioRxiv - Neuroscience 2022Quote: ... Capillaries were identified by immunostaining with rat monoclonal anti perlecan antibody (1:100, clone A7L6, Millipore) overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rat secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: Rats were transcardially perfused with 4% paraformaldehyde (PFA) in 0.1M PBS (PBS tablets Sigma P-4417). Brains were immediately frozen in 2-methylbutane (isopentane ...
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bioRxiv - Cell Biology 2024Quote: Hep3B cells were cultured on coverslips with collagen (Type I solution from rat tail, Sigma C3867) and fixed in fixation buffer B (2.5% glutaraldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... substrates were coated with 10 μg/cm2 collagen type-I from rat tail (Sigma, Cat # C3867) or 0.2ug/mL E-selectin (Cat# 724-ES ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa 488 rat anti-mouse NeuN (cat # MAB377X, Millipore sigma, clone A60, 5 ng/μl). After incubation ...
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bioRxiv - Neuroscience 2023Quote: ... 56-day-old rats received an injection of pilocarpine hydrochloride (340 mg/kg ip; Sigma-Aldrich) 20 min after being injected with atropine sulfate (5 mg/kg ip ...
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bioRxiv - Neuroscience 2023Quote: ... Rats received an intraperitoneal administration of 0.2 mg/kg/day PPX (Sigma–Aldrich, St. Louis, MO), diluted in 0.9% NaCl ...
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bioRxiv - Immunology 2023Quote: Cell seeding on multiwell plates: T84 cells were seeded on rat-collagen (Sigma-Aldrich #C7667-25MG) coated multiwell plates (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... SD rats (aged 8-15w) were synchronized via injection of 40µg of LHRHa (L4513, Sigma-Aldrich). About 94-96h later ...
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bioRxiv - Neuroscience 2023Quote: ... rats received cocaine (0, 2.5, 5, or 10 mg/kg, i.p.; Sigma–Aldrich, St. Louis, MO) and were confined to the opposite side compartment for 1 h ...
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bioRxiv - Physiology 2024Quote: ... all rats received an IP bolus injection of filtered 99% D2O (Sigma Aldrich, St. Louis, MO) equivalent to 5% of body water (estimated at 60% of body mass) ...
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bioRxiv - Physiology 2024Quote: ... Cultures plates were coated with collagen (Collagen, Type 1 from rat tail, Sigma, Saint Louis, MO) diluted 1 to 50 ratio in sterile H2O prior to cell seeding ...
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bioRxiv - Plant Biology 2023Quote: 6xHistidine-Glutathione-S-transferase (GST) tagged proteins of the predicted intracellular region of SOBIR1 (termed the KD) was cloned into pCDF-Duet vector (Novagen, EMD Chemicals, San Diego, CA) and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1:500 was used to detect myc tagged ATR and incubated at room temperature for 1 h in blocking buffer (1 % BSA [Sigma], 0.2 % Tween-20 in 1x PBS) in a wet chamber ...
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bioRxiv - Biochemistry 2021Quote: ... and purified using DDDDK-tagged protein purification gel (MBL, Japan) followed by elution with 0.1 mg/ml FLAG Peptide (Sigma-Aldrich Co., St. Louis, Missouri, the US). To remove the flag peptide ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... T cells were then infected with HIVBaL and cultured for up to 7 days in RPMI medium additionally supplemented with 10 U/ml recombinant interleukin-2 (Sigma). The dividing cell lines HEK293T (ATCC ...