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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were sonicated and protein concentration was determined using BCA protein assay (Sigma). 30 μg of total proteins were loaded on 12% SDS–PAGE gel and separated by electrophoresis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... a marker of protein oxidation (protein carbonyls; OxyBlot; s7150; EMD Millipore, Billerica, MA), and a marker of mitochondrial biogenesis (PGC-1α ...
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genomics 2019Quote: Duolink PLA kit was used to detect protein-protein interactions (Sigma Aldrich DUO92101). PLA was performed according to manufacturer’s protocol plus additional negative controls ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Immunology 2020Quote: Total proteins isolated from BMDCs were lysed in CytoBuster protein extraction reagent (Novagen). Protein quantification was performed using NanoDrop One Spectrophotometer ...
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bioRxiv - Cell Biology 2022Quote: ... the other stained for proteins with SYPRO Red Protein Gel Stain (Sigma Aldrich). Both dyes were used according to the manufacturers’ protocols.
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bioRxiv - Molecular Biology 2024Quote: The protein mixtures were filtered using a low protein binding filter (Millipore Sigma) and purified on a SourceQ15 anion exchange column (Cytiva ...
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bioRxiv - Genomics 2024Quote: ... Protein transfer and total protein content was assessed by Ponceau Staining (Sigma Aldrich). Ponceau Stain was washed out with 1x TBST ...
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bioRxiv - Genetics 2024Quote: ... protein concentration was measured by use of a BCA Protein Assay (SIGMA Aldrich) and samples stored at -80°C.
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bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Microbiology 2023Quote: ... 1000 µg of protein extracts were cleared with agarose protein A beads (Millipore) at 4 ℃ for 1.5 h on a rocking platform ...
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bioRxiv - Microbiology 2023Quote: ... or with slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose). For GFP-p65 and GFP-COMMD2 the samples were immunoprecipitated as per manufacturer’s instructions with the elution was recovered in either 5x with Laemmli buffer 74 and boiled at 98°C (dry bath ...
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bioRxiv - Plant Biology 2023Quote: ... the protein extract was incubated with 30 μL Protein A Agarose beads (Millipore), 10 μL anti-SIZ1 antibody with rotation at 4°C for 4 h.
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bioRxiv - Microbiology 2023Quote: ... The protein was purified using a Protein A agarose gravity column (Millipore Sigma) followed by SEC using a S200 column.
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Proteins were loaded on PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Samples were heated at 100 °C for 10 min in a 4X loading buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... ii) 300 μg C g−1 13C-labelled Algal crude protein extract-98 atom% 13C (ISOTEC, Sigma-Aldrich), iii ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA-cross-linked proteins were immunoprecipitated (1% kept as input) using 5 μg of NF-kB/p65 (Millipore) or control mouse IgG antibody ...
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bioRxiv - Neuroscience 2019Quote: ... 700-1000 µg total protein was incubated overnight at 4°C with 1:100 anti-FLAG (Sigma, F1804) or anti-Myc (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2, Millipore Sigma #04-263) at a dilution of 1:400 for 3 hours at room temperature.
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bioRxiv - Biochemistry 2020Quote: ... Lamin proteins were detected using mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR) ...
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bioRxiv - Microbiology 2021Quote: ... MERS S-protein was detected using a monoclonal anti-FLAG M2-HRP conjugated antibody (SIGMA, A8592 @ 1:2000) which bound to a C-terminal FLAG-tag ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of EGFP fusion proteins was then induced by addition of 1 µg/mL doxycycline (Sigma-Aldrich, D9891) and EGFP expressing cells were positively selected by FACS ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Physiology 2020Quote: ... and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000, Sigma, #S8809), the anti-transferrin receptor mouse monoclonal antibody (TransR ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then analyzed by western blotting using a primary anti-biotin antibody (1:5,000 dilution; Sigma-Aldrich) and an anti-mouse secondary antibody coupled to peroxidase (1:10,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were transferred to Eppendorf protein LoBind tubes and stained with DAPI (1 μg/mL, Sigma-Aldrich). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... and grown at 37°C to an optical density (600 nm) of ~0.6-0.8 in Super Broth before protein expression was induced with 1 mM IPTG (Sigma) overnight at 18°C ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1 µg/µl total protein using an ANTI-FLAG M2 Affinity Agarose Gel (Sigma). Beads were centrifuged and washed three times in lysis buffer and eluted and denatured (95 °C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to PVDF membranes and probed with M2 anti-FLAG antibody (Sigma; 1 in 5,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
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bioRxiv - Cell Biology 2022Quote: Purified GFP-Cdt1 protein (1.6 mg ml-1) was treated with CDK1-CyclinB Kinase (EMD Millipore, # 14-450) supplemented with 10 mM ATP in kinase buffer (20 mM Tris pH 8 ...
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bioRxiv - Neuroscience 2022Quote: Rabies injected brains were immunohistochemically stained using antibodies against the 2A linker protein (Merck/ Millipore, ABS31, 1:2000) found in tTA expressing cells ...
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bioRxiv - Neuroscience 2022Quote: ... and immunoblotted for protein expression using the following antibodies: guinea pig anti-GLT1 at 1:25,000 (Millipore AB1783) and mouse anti-beta actin at 1:20,000 (Sigma-Aldrich A1978) ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-glial fibrillary acid protein (GFAP) (1/1,000; Sigma-Aldrich, St. Louis, MO, USA; Cat. No. G3893); mouse anti-S100β (1/1,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Half fresh medium containing Amyloid β Protein Fragment 1-42 (50 nM; Sigma-Aldrich, St. Louis, MO, USA) or DMSO (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500, AB5804, Millipore, Lot # TA265137) or goat anti-Iba1 (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... Protein was concentrated to 1 ml using a Amicon Ultra-4 Ultracel 30 kDa spin concentrator (Merck Millipore) and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to nitrocellulose membranes and probed with 1:1,000 a-FLAG primary (Sigma-Aldrich, clone M2) and 1:5,000 a-mouse secondary (Thermo Fisher ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to PVDF membrane and probed with M2 anti-FLAG antibody (Sigma; 1 in 1,000 dilution) and HRP-conjugated goat anti-mouse antibody (1 in 250,000 dilution) ...
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bioRxiv - Molecular Biology 2019Quote: ... Band sizing was performed using the ImageJ 1.5 software (http://rsb.info.nih.gov/ij) and relative protein expression was determined by normalizing to GAPDH (1:10,000, MAB374, Millipore, Bedford, MA), for cell samples ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...