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Citations for Millipore Sigma :
2151 - 2200 of 3014 citations for 6 Methoxyquinazoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Cold APC purification buffer (100 mM NaPi, Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333, 5 mM MgCl2 × 6 H2O, Sigma#M2670 ...
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bioRxiv - Developmental Biology 2019Quote: ATDC5 were plated in 6-well plates to 80% confluency and lentiviral transduction was using diluted viral media with 0.1% polybrene (EMD Millipore) for 24 hours followed by selection with 4μg/ml Blastocidin and Puromycin for 5 days post transfection ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were washed three times in PBS and mounted in 70% glycerol with with 5 μgml-1 concentration of 4-6-diamidino-2-phenylindole (DAPI, Sigma) and cells were mounted on cover slips ...
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bioRxiv - Molecular Biology 2019Quote: Bloodstream parasites were spun down and resuspended in differentiation media (19) supplemented with 6 mM cis-aconitate (Sigma-Aldrich A3412) at 27°C for three days.
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bioRxiv - Biochemistry 2019Quote: ... 3 mM KCl) and harvested in a PBS lysis buffer (PBS plus 6 uL/mL protease inhibitors cocktail (Sigma P8340), 0.6 mM PMSF ...
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bioRxiv - Genomics 2019Quote: ... 1.5 g/L (days 0-5 and days 11-28) or 2.5 g/L (days 6-10) NaHCO3 (Sigma-Aldrich), and 0.25 mM (days 3-10 ...
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bioRxiv - Neuroscience 2019Quote: 12 female young adult C57BL/6 mice (mean age 2.2 months) were sedated intraperitoneally with chlorprothixene (0.5 mg/kg, Sigma-Aldrich, UK) and anaesthetised with isoflurane (2% for induction ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... pH 7.4) containing 10 mM sodium azide and 6 mM 2-deoxy-D-glucose (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 50 min ...
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bioRxiv - Cancer Biology 2019Quote: ... 6 week-old mice were injected intraperitoneally three times at one day interval with tamoxifen (T5648; Sigma-Aldrich, Bornem, Belgium) dissolved in corn oil at a concentration of 30 mg/mL and at a dose of 150 mg/kg of body weight ...
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bioRxiv - Immunology 2021Quote: ... Monolayers were then rinsed thoroughly with Promocell DC generation medium to remove non-adherent cells and subsequently were cultured for 6 days in dendritic cell generation medium supplemented with cytokines (Sigma) to generate immature MoDCs.
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured for different periods (6, 12 or 24 hours) in different combinations of vascular endothelial growth factor (VEGF, human recombinant; Millipore) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Neuroscience 2020Quote: ... Spontaneous IPSCs were isolated by blocking AMPA receptor-mediated events with CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 20uM; Sigma-Aldrich). Miniature events were isolated by blocking sodium channels with the addition of tetrodotoxin (1uM ...
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bioRxiv - Microbiology 2021Quote: Plaque size analysis were performed in HEK293T Spir-1 WT and KO cells seeded in 6-well plates coated with poly-D-lysine (Sigma). Once confluent ...
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bioRxiv - Neuroscience 2020Quote: ... two brains were pooled (n=6) and homogenized in 300 µL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). Tissue preparation and biochemical analysis followed protocols described in (Sachett et al. ...
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bioRxiv - Biophysics 2020Quote: ... Dead cells were removed on day 6 after T-cell isolation by means of density-dependent gradient centrifugation (Histopaque 1119, Sigma). Antigen-experienced T-cells were used for experiments on day 7 – 9.
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bioRxiv - Cancer Biology 2021Quote: ... cells were plated in 6-well plates and stained/fixed with 2.5mg/mL solution of crystal-violet (Sigma, Cat #C3886) in 20% methanol.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were trypsinazed and plated at a density of 25000 cells/ml in 6-multiwell plates in DMEM (Sigma, UK), supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626), 0.4 mg/L biotin and 0.5% methanol ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: Frozen skin tissues from a normal individual and the patients were sectioned at 6 μm and stained with mouse monoclonal anti-NC1 C7 antibody (clone LH 7.2; Sigma-Aldrich) at a 1:1000 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Trx-His(6)-hSLAP2 or Trx-His(6)-Δlinker-hSLAP2 were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Neuroscience 2021Quote: ... three brains were pooled (n=6) and homogenized in 450 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
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bioRxiv - Neuroscience 2020Quote: ... 6-month-old littermate matched were injected subcutaneously with vehicle [40% (w/v) (2-hydroxypropyl)-β-cyclodextrin (Sigma– Aldrich #332607)] or MLi-2 dissolved in vehicle at a 30 mg/kg final dose ...
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bioRxiv - Neuroscience 2020Quote: ... We mechanically dissociated the tissue in a glass homogenizer with 6 ml of aCSF-based Homogenization buffer [aCSF plus 10 mM HEPES (Sigma) to maintain stable pH despite CO2 released from the cells ...
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bioRxiv - Bioengineering 2021Quote: ... the pellets were collected to examine the cell lysis interference using a Glucose-6-Phosphate Dehydrogenase kit (Sigma-Aldrich, USA). The details of EPS extraction and analytical methods for various EPS components (proteins ...
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bioRxiv - Cell Biology 2021Quote: ... G6P concentrations were determined at 2 hrs and 6 hrs post activation and compared to unactivated controls using a G6P kit (Sigma) and following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: HDMB03 cells (3×105 in 6-well plates) were treated with global de-methylating agent 5-AzaC (5 μM) (5-Aza-2-deoxycytidine; Sigma). Following 96 hours of incubation ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in 1% normal donkey serum in PBS with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were released from the second thymidine block for 6 hours prior to the addition of Flavopiridol (1 μM; Sigma). After 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... after which we incubated a 20µL pre-isolation aliquot of the filtrate with 0.1µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Millipore-Sigma, cat. #D9542) and mounted on a slide for imaging (Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... after which we incubated a 20µL pre-isolation aliquot of the filtrate with 0.1µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Millipore-Sigma, cat. #D9542) and mounted on a slide for imaging (Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... In some cases mononuclear cells were cultured as mammospheres in 6 cm culture plates coated with polyhydroxyethylmethacrylate (12 mg/ml, Sigma), under hypoxic conditions (7% O2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Lung tumors were induced in 6-8 weeks old mice by intraperitoneal injection of 8 doses of 1 g/kg of urethane (ethyl carbamate; Sigma); second dose was given 48h after the initial one and then once a week to reach a total of 8 doses ...
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bioRxiv - Cell Biology 2021Quote: C57BL/6N female mice (4-6 weeks old) were superovulated by injection with 5 IU of pregnant mare serum gonadotropin (PMSG) (Millipore), followed 5 IU of human chorionic gonadotropin (hCG ...
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bioRxiv - Cell Biology 2021Quote: ... and RANK-L (100 ng/ml) for 4 to 6 days followed by staining for Tartrate-resistant acid phosphatase (TRAP) using a kit (Sigma) per manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and antigen retrieval was performed in 37 °C trypsin (pH 7.8) for 1 h or 10 mM sodium citrate buffer (pH 6) (Sigma-Aldrich) at 95 °C for 10 min ...
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bioRxiv - Physiology 2020Quote: ... HMDC (passage 7-9) were seeded onto plastic 6-well fibronectin-coated (10 μg/ml in PBS, Sigma-Aldrich, UK) culture plates (described below ...
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bioRxiv - Molecular Biology 2020Quote: ... Nucleofected cells were plated onto duplicate wells of Matrigel®-coated 6-well culture plates for 24 h before adding 2.5μg/mL puromycin dihydrochloride (Sigma-Aldrich) for selection ...
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bioRxiv - Plant Biology 2020Quote: ... 6 to 7 day old light or dark grown seedlings were pre-treated with 50 μM cycloheximide (CHX, Sigma Aldrich) or with 50 μM proteasome inhibitor Bortezomib (Selleckchem) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The washed cell pellets were resuspended into 6 mL of DM29 with the addition of 60 μL of pefabloc (100 mM, Sigma) and 6 μL of DNase I (20 mg mL-1 ...
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Microbiology 2020Quote: ... and bacterial nucleoids stained by topping cells with a drop of Fluoroshield™ containing 1.5 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Cover slips were placed over the samples and kept at room temperature for ∼ 30 minutes before cells were imaged.
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bioRxiv - Microbiology 2021Quote: ... Exponentially growing cells pre-cultured in the presence or absence of Mn were harvested and resuspended at a density between 1E+07 and 2.5E+07 cells/mL in their corresponding growth medium supplemented with erythromycin (5μg/mL) and 10μM 5(6)-carboxyfluorescein (Sigma-Aldrich 21877), with or without Mn (20μM) ...
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bioRxiv - Microbiology 2021Quote: ... trypsinized and analysed on a Guava EasyCyte 6/2L bench cytometer equipped with 488 nm and 532 nm lasers (Millipore), for detection of GFP-positive cells and dextran-positive cells ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were fixed using 6% formaldehyde/PBS solution for 10 minutes and stained with 1% crystal violet (Sigma Chemical Corp) in 70% methanol for 1 hour ...
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bioRxiv - Physiology 2020Quote: ... Dead cells and debris were excluded by measurements of forward-versus side-scattered light and DAPI (4′,6-diamino-2-phenylindole) (Sigma) staining ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mg/L avidin and 6 mM MgCl2 were added to the supernatant to improve the Strep tag binding efficiency with Strep-Tactin beads (Novagen). Next ...
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bioRxiv - Systems Biology 2020Quote: ... TMT labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (Sigma) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... parasites at 32 hpi and 38 hpi were cultured at 37 °C or 40 °C for 6 h in the presence or absence of 20 μM Wortmannin (Selleckchem, Houston, TX) or 40 μM LY294002 (Sigma). The cells were subsequently washed and resuspended in complete medium (1% hematocrit) ...