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Citations for Millipore Sigma :
2101 - 2150 of 3014 citations for 6 Methoxyquinazoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Excised tissues are first allowed to fold into spheroids and seal in HM for 3-6 hours and are then embedded in 0.5% low gelling point agarose (Sigma) prepared in HM to reduce tissue movement during imaging ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... A 30-min incubation of PBS supplemented with 1 µg/mL of 4′,6-diamidino2-phenylindole (DAPI) (Sigma-Aldrich) was performed ...
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bioRxiv - Developmental Biology 2024Quote: ... and seeded at 8X105-1X106 cells per well in a 6 well plate coated with Matrigel (Millipore Sigma, CLS354277) 24 hours prior to differentiation treatment ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg viral plasmid were diluted in 6 μL X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich, 6365787001) and 150 μL DMEM and incubated at room temperature for 15 min before being added to the cells ...
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bioRxiv - Cell Biology 2024Quote: ... we use primary anti-acetylated tubulin antibody from mouse (monoclonal 6-11 B-1, T7451 Sigma, 1/1000 dilution) as well as primary anti-tyrosinated tubulin antibody from mouse (monoclonal TUB-1A2 ...
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bioRxiv - Neuroscience 2024Quote: ... and the remaining pellet was washed once with 50 mM TBS, centrifuged (200,000x g, 22 min at 4 °C) and then solubilized in 6 M GuHCl (Sigma) with protease inhibitors ...
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bioRxiv - Neuroscience 2024Quote: ... and then immersed in OCT for the preparation of transversal cryosections (10µm) that were mounted on microscope slides treated with 6% silane (cat#440140, Sigma). Electroporated retinas ...
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bioRxiv - Pathology 2024Quote: ... of mosquito EV-lipid extract or of EV-lipid fraction 6 was treated with 1 U of SMase (Sigma) for 1h at 37°C ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... 250,000 cells per well were seeded in 6-well plates 24 hours before adding MG- 132 (C2211, Sigma-Aldrich) at a concentration of 0.375 µM dissolved in DMSO ...
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bioRxiv - Microbiology 2024Quote: The 10-ng internal CL standard (14:0-14:0-14:0-14:0) (Sigma-Aldrich, 63988-21-6) was added to lipid extracts for lipidomic analyses ...
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bioRxiv - Biochemistry 2024Quote: Proteins were extracted from 300,000 to 400,00 HUVEC cells (per one well of a 6-well plate) with RIPA buffer (Millipore Sigma) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... seedlings were grown in MS media supplemented with sucrose and various concentrations of 6-benzylamino purine (BA, Sigma, No. B3408) or sodium hydroxide as control.
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bioRxiv - Cancer Biology 2021Quote: ... before the addition of MNNG to inhibit the repair of O6meG adducts by O-6-methylguanine-DNA methyltransferase (MGMT, Sigma). After 48h MNNG treatment ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibodies were washed away with PBS (3x 5 min) whereafter 4’,6-diamidino-2-phenylindole (DAPI, 1:500, Sigma) was added for 10 minutes to localize the cell nuclei ...
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bioRxiv - Biophysics 2020Quote: Purified K2P2.1cryst was concentrated to 6 mg ml-1 by centrifugation (Amicon Ultra-15, 50 kDa molecular mass cut-off; Millipore) and crystallized by hanging-drop vapor diffusion at 4 °C using a mixture of 0.2 μl of protein and 0.1 μl of precipitant over 100 μl of reservoir containing 20–25% PEG400 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed 2× in PBS and then counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1:10 000; Sigma) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was changed to S1 medium consisting of basal medium supplemented with 6 μM CHIR99021 (Sigma, Cat No. SML1046). Basal medium was prepared by adding 1x GlutaMax supplement (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 was used in all experiments except where indicated (mouse ...
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bioRxiv - Neuroscience 2021Quote: ... saline or 6-OHDA (5 μg/μL; 2 μL in total, at a rate of 0.5 μL/min, Sigma, Canada) were injected unilaterally in the dorsal striatum ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, cat. D8517, Sigma Aldrich, St. Louis, MO, USA) diluted 1:3000 in PBS 1X and then mounted with Vectashield antifade medium (cat ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed using 70% Ethanol and re-suspended in PBS containing 1 μg/ml 4’,6-diamino-2-phenylindole (DAPI, Sigma) and analyzed by microscopy ...
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bioRxiv - Microbiology 2020Quote: ... cells were analyzed using a Guava EasyCyte 6/2L bench cytometer equipped with 488 nm and 532 nm lasers (Millipore). Flow cytometry plots are representative of at least three independent experiments.
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bioRxiv - Microbiology 2020Quote: ... Infected cultures were then either trypsinized for detection of GFP-positive cells and/or dextran-positive cells by flow cytometry on a Guava EasyCyte 6/2L bench cytometer (Millipore), or fixed with 4% paraformaldehyde and analyzed by fluorescence microscopy after labeling with antibodies specific for UIS4 (Sicgen ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were rinsed several times in PBSDT and endogenous peroxydase was inactivated by a treatment with H2O2 6% (Sigma, H1009) during 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... and the supernatant was gently layered on a discontinuous Percoll gradient (2 %, 6 %, 10 %, and 20 % v/v in Tris-buffered 0.32 M sucrose; Sigma-Aldrich). After centrifugation at 33’500 g for 5 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 μL (corresponding to 4.106 cells/cm2) of suspension was plated on 6 cm plates filled with 2 mL of 2% Phytagel (Sigma-Aldrich) as previously described by Dubravcic et al ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was titrated into 2 × 105 PC-3M cells on 6-well plates with 2 mL complete RPMI-1640 supplemented with 8 μg/mL polybrene (Sigma). After overnight infection ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared and then immediately enzymatically treated at 34°C with an initial incubation in a papain (6 mg/mL, Sigma) and dithioerythritol (10 mg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... For some experiments the AMPA/kainate glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 μM, Sigma-Aldrich), the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were grown for 48 h in the presence of 7 µM ouabain (Sigma, O3125; CAS:11018-89-6), then diluted into 14 cm-dishes and grown for 2-3 weeks in the presence of ouabain to obtain isolated clones ...
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bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
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bioRxiv - Molecular Biology 2021Quote: ... CLIP-UPF1 stable cell lines or a GFP-expressing control line were seeded in 6 × 15 cm plates and then treated with 200 ng/mL doxycycline hyclate (Sigma) for 48 hours to induce CLIP-UPF1 expression ...
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bioRxiv - Developmental Biology 2022Quote: ... in PBST and then counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 0.001 mg/mL in 1x PBS, Sigma-Aldrich) for 5 min in the dark ...
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bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (5 μM) and propidium iodide (PI) (50 μg/mL) (Sigma-Aldrich, St. Louis, USA) and incubated at room temperature for 30-60 minutes ...
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bioRxiv - Genomics 2020Quote: Electroporations were performed in 2-4 biological replicates into p2L RAR-DamA126 cells by mixing the listed DNA amounts with 5-20*10^6 cells in 300 ul EmbryoMax Electroporation Buffer (Millipore) and electroporating in a 0.4-cm electroporation cuvette using a BioRad electroporator at 230 V ...
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bioRxiv - Immunology 2021Quote: ... cells were seeded 24 hours prior to transduction at a density of 50,000 cells per well in 6-well plates and transduced with lentiviral vectors in the presence of 5 µg/ml polybrene (Millipore). When cells were transduced with a Pro-Code library ...
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bioRxiv - Immunology 2021Quote: Blood was extracted from hBLT mice at 3 and 5-6 weeks post-infection and lysed with Red Blood Cell Lysis Buffer (SIGMA). T cells were activated for 1.5 h with 5μl/ml of anti-CD28 and anti-CD49d in the presence or absence of 6.4μg/ml of a Gag pool of peptides in the presence of 0.5μg/ml Brefeldin A ...
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bioRxiv - Immunology 2021Quote: ... 1-2 x 106 mononuclear cells isolated from blood or tonsil samples were cultured in a round bottom 96 well plate at 37°C 5% CO2 in R10 media for 6 hours with or without 200 ng/ml phorbol myristate acetate (PMA) and 1.0 μg/ml Ionomycin (Sigma-Aldrich) or 0.5 μM/ml 5-amino-6-D-ribitylaminouracil (5-A-RU ...
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bioRxiv - Molecular Biology 2021Quote: ... 4×106 ESCs were allowed to attach to gelatinised 15cm dishes for 6-8 hours and treated with 1μM all-trans retinoic acid (Sigma-Aldrich) in EC-10 medium (DMEM supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2019Quote: Embryos were incubated in 1:5000 acridine orange solution for 1 hr at 28.5°C (stock: 6 mg/ml, Sigma-Aldrich) followed by 2X washes in fish system water ...
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bioRxiv - Immunology 2019Quote: ... adult animals (6-12 weeks) were perfused with 25 mL of PBS (Hyclone) and 25 mL of 4% paraformaldehyde (PFA, Sigma) sequentially at room temperature (RT) ...
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bioRxiv - Neuroscience 2019Quote: ... mononuclear cells (1×106 cells/mL) were cultured for 6 h with or without phorbol 12-myristate 13-acetate (PMA, 50ng/mL; Sigma), ionomycin (50ng/mL ...
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bioRxiv - Biophysics 2019Quote: ... The reaction was started by adding 20 µl of 500 µM DHF (Sigma Aldrich, cat# D7006, CAS: 4033-27-6) in MTEN with 5 mM DTT to 180 µL of pre-reaction mixture ...
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bioRxiv - Cell Biology 2019Quote: ... Covalently modified αSYN was separated from non-reacted dye on a Sephadex G-25 column (Amersham, GE) and concentrated with 6 kDa MW cut-off spin columns (Millipore). The final concentration of the fluorescently labeled protein was measured by UV-VIS spectrophotometry at 280 nm with E ...
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bioRxiv - Bioengineering 2019Quote: Cy3-(GT)6-SWCNTs and Cy3-(GT)30-SWCNTs were first filtered 3 times using 100kDa Amicon centrifuge filters (Millipore) to remove free Cy3-DNA from solution ...
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bioRxiv - Bioengineering 2019Quote: ... Laccase activity was followed by the oxidation of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (Sigma-Aldrich, USA) (33) ...
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bioRxiv - Cancer Biology 2020Quote: Cre expression and activity was induced in 6-8 week old Krt19-CreERT and Rosa26-CreERT mice by intraperitoneal injection of tamoxifen (Sigma) dissolved in corn oil (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... Viral size particles in the filtrate were concentrated to ~500 mL (viral concentrate) using tangential flow ultrafiltration with a 30kDa MW prep-scale Spiral Wound TFF-6 cartridge (Millipore) (53).