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Citations for Millipore Sigma :
2001 - 2050 of 3014 citations for 6 Methoxyquinazoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 50 μl of antibody exposed to hemin or hemin in PBS only were mixed with 150 μl of reaction buffer (0.15 M citrate-phosphate buffer pH 5) containing 0.9 mM of 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma-Aldrich) and 6 mM H2O2 ...
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bioRxiv - Plant Biology 2021Quote: ... For Co-IPs with MED10B-MEC-ProtA we used IgG Sepharose® 6 Fast Flow (Sigma, GE17-0969-01) beads and increased washing steps (1xIP buffer ...
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bioRxiv - Genomics 2019Quote: ... Antigen unmasking was performed boiling sections on 10 mM citrate buffer pH 6 for 10min and tissue was permeabilized with PBS 0.5% Triton X-100 (Sigma). Blocking was performed by 1h incubation in humid chamber with 3% of normal donkey serum (Vendor ...
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bioRxiv - Immunology 2020Quote: ... one 10-cm dish of packaging cell line at roughly 70% density was transfected with the target construct and two supplementing vectors providing the VSV gag/pol and VSV-G-env proteins (6 μg of DNA each) using polyethylenimine (22.5 μg/ml, Sigma) and Polybrene (4 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated for 6 h with PMA (10 ng/mL) and ionomycin (500 ng/mL) (both from Sigma), in the presence of GolgiStop plus GolgiPlug (both from BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Fractions containing the complex were concentrated to 6 mg/mL using a 10 kDa Amicon® spin concentrator (Millipore). 3 μL of the complex was mixed with 1 μL of a n-Dodecyl-β-D-Maltopyranoside (DDM ...
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bioRxiv - Neuroscience 2019Quote: ... The oxygen electrodes were calibrated in air-saturated 0.9% sodium chloride (at 37°C) and oxygen-free standard solution [0.1M sodium hydroxide (SX0607H-6, Sigma-Aldrich) and 0.1M sodium ascorbate (A7631 ...
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bioRxiv - Biophysics 2019Quote: ... freshly purified tau was first reduced with 1 mM DTT for 10 minutes and buffer exchanged into a labeling buffer (20 mM Tris pH 7.4, 50 mM NaCl, and 6 M guanidine HCl) using Amicon concentrators (Millipore) (17) ...
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bioRxiv - Genetics 2019Quote: ... Chromosomes were counterstained with 0.5 µg/mL DAPI (4′,6-diamidino-2-phenylindole; Sigma-Aldrich, St. Louis, MO, USA) in 1× PBS at RT for 15 min ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were rinsed four times with cacodylate buffer and post-fixed with 0.8% K3Fe(CN)6 (Sigma-Aldrich) and 1% OsO4 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... BL cells were spun down and plated at 0.5 × 10^6 cells in fresh media containing soluble anti-IgM or anti-IgG (Sigma) at concentrations and times indicated in figure legends (2.5-5 ug/mL for 22-48 hrs).
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bioRxiv - Microbiology 2021Quote: ... all water samples were kept on ice for approximately 6 hours and filtered through a 0.22 μm Sterivex GV filter (Millipore) using a Masterflex L/S 7553-70 peristaltic pump ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cells grown on 6-well culture plates using lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were washed with TBST and incubated with 300 nM 4‘,6-diamidino-2-fenilindol (DAPI, D9542, Sigma-Aldrich) for 1 minute ...
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bioRxiv - Cancer Biology 2021Quote: ... a pool of T cells was stimulated for 6 hours with 50ng/ml PMA and 1μg/ml Ionomycin (Sigma). T cells were then harvested and washed with FACS Buffer and stained for surface markers followed by fixation and permeabilization using BD Cytofix/Cytoperm Fixation/Permeabilization Solution (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: ... Cells were plated at a density of 0.8 × 106 cells per well on 6-well plates pre-coated overnight with 0.1 mg/ml poly-D-lysine (Millipore) and one third of the media in each well was replaced every 3 days ...
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bioRxiv - Cancer Biology 2020Quote: ... at an age of 6-10 weeks by a single intra peritoneal injection of 2 mg Tamoxifen (Sigma; T5648) in corn oil (Sigma ...
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bioRxiv - Biophysics 2020Quote: Immunoprecipitation samples were fractionated on 6% or 15% SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF) (Millipore). Membranes were blocked in TBS solution (0.05% Tween-20 in PBS containing 5% milk) ...
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bioRxiv - Cell Biology 2020Quote: Mitochondria were isolated from a total of ~60 million (30×6-well) cells by manual cell homogenization in a specialised buffer (20 mM HEPES (pH 7.6) (Sigma), 220 mM D-mannitol (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... > 6-weeks old mice were injected with 100□μl of tamoxifen (10□mg /□ml in corn oil) (Sigma, #T5648) via intraperitoneal (i.p. ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with shRNA-encoding lentivirus supernatants in the presence of 6 μg/ml polybrene (hexadimethrine bromide; Sigma-Aldrich). Twenty-four hours after infection ...
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bioRxiv - Microbiology 2019Quote: ... or adult control mice (n = 6) received an intra-peritoneal (IP) injection of 1.25mg/kg LPS from Escherichia coli 0111:B4 (Sigma), 0.5mg/kg murine Tumour Necrosis Factor Alpha (TNF-ɑ ...
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bioRxiv - Microbiology 2020Quote: ... 4 mM stable glutamine without sodium pyruvate (Biochrom) and supplemented with 6 %inactivated fetal calf serum (iFCS) (Sigma-Aldrich). HeLa cells were cultured in DMEM containing 4.5 g × l−1 glucose ...
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bioRxiv - Microbiology 2021Quote: ... 107 RAW264.7 cells uninfected or infected with UVi(MNV) or MNV at MOI 10 for 6 and 10h were treated with 100µg/mL of cycloheximide (CHX-Sigma) for 3min at 37°C to lock the translating ribosomes onto the transcripts before being placed on ice ...
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bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-acetylated tubulin (clone 6-11-B1, Sigma-Aldrich, T6793; 1:1,000), mouse-anti beta-actin (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... and non-selective P2-purinoreceptor antagonist pyridoxalphosphate-6-azophenyl-2’,4’-disulfonic acid (PPADs) ([25mg/kg] in PBS; Sigma).27,44–46 Following recording of a pre-inhibitor response ...
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bioRxiv - Cell Biology 2021Quote: ... 6 h after the second thymidine release cells were transferred into medium containing 8 μM RO3306 (Sigma-Aldrich, SML0569) for 4 h ...
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bioRxiv - Immunology 2022Quote: ... The infected monolayers were left for 6-8 days in a 35°C incubator under 0.8% methylcellulose (Millipore Sigma) overlay (in EMEM medium with 10% FBS ...
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bioRxiv - Biophysics 2022Quote: ... Elutions were pooled down and concentrated using Vivaspin 6 centrifugal unit with 30 kDa cut off membrane (EMD Millipore) before being loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (Cytiva ...
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bioRxiv - Biophysics 2022Quote: ... Elutions were pooled down and concentrated using Vivaspin 6 centrifugal unit with 30 kDa cut off membrane (EMD Millipore) before being loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (Cytiva ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... with a cocktail of functionally inhibitory integrin blocking antibodies (20 μg/mL anti-α3, clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6 ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... 10 µL of the HDX reaction were removed and quenched by diluting 1:6 into 0.75% formic acid (FA, Sigma), and digested for two min with porcine pepsin (0.25 mg/mL ...
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bioRxiv - Bioengineering 2022Quote: ... poured into a sterile 750mL bottle along with 6% w/v PEG-8000 (Sigma-Aldrich, cat. code P2139-500G) and 3% w/v NaCl (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Immunology 2022Quote: ... 6 mmol/L l-glutamine (BioWhittaker®) and a mixture of penicillin/streptomycin (100U/100 µg/mL, Sigma®) at 37°C in the presence of 5% CO2 ...
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bioRxiv - Plant Biology 2022Quote: A stock of 5-(and-6)-carboxyfluorescein diacetate (CFDA) was prepared by dissolving 5 mg/ml CFDA (Sigma Aldrich) in acetone ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 6% rabbit serum (Pel-Freez, 31125-5) and 10 ug/mL gentamicin (Sigma-Aldrich, 1405-41-0) to maintain GFP-tagged B ...
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bioRxiv - Biochemistry 2024Quote: ... homogenised in 6 mL of 2:1 (v/v) chloroform:methanol containing 0.01% (w/v) butylated hydroxytoluene (BHT, Sigma-Aldrich) as antioxidant ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were seeded on 6-well plates (∼400,000 cells/well) coated with 0.1% gelatin (Sigma-Aldrich cat# G1393), and transfected ∼24 h later using the calcium phosphate method ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred to 6-well low adhesion plates in ESC media supplemented with 10 µM ROCK Inhibitor (Sigma; Y0503) to initiate differentiation ...
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bioRxiv - Molecular Biology 2024Quote: ... In the tMCAO study the brains were cut in 6 coronal sections and stained with 1% 2,3,5-Triphenyltetrazolium Chloride (TTC) (Sigma-Aldrich) in PBS solution for 5 minutes at 37 °C in agitation before dissection of contralateral and peri-ischemic cortical regions ...
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bioRxiv - Cancer Biology 2024Quote: ... Transient transfection with mutant MLH1 constructs (purchased from TWIST Biosciences) and siRNA against CDK4/6 (purchased from Sigma-Aldrich) was conducted using JetPrime PolyPlus transfection reagent ...
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bioRxiv - Cell Biology 2024Quote: ... 561 and 640 nm laser lines were used for excitation of DAPI (4rn,6-Diamidino-2-phenylindole, Sigma-Aldrich), Alexa Fluor 555 and Alexa Fluor 647 respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: Adult female flies aged 2–6 days post eclosion were head-fixed to a custom mount using eicosane (Sigma). Cuticle ...
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bioRxiv - Neuroscience 2024Quote: ... cortical neurons were plated at a density of 300,000 on 6-well plates coated with a solution of 0.0005% poly-l-ornithine and laminin (2.5 μg/ml) (Sigma Aldrich). Neurons were kept in supplemented Neurobasal at 37°C and 5% CO2 for 14-16 days (DIV 14-16).
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM Glucose) containing 1µg/mL of 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... 6-week-old animals were treated twice with 500 mg/kg body weight tamoxifen (Sigma-Aldrich, cat# T5648-5G) dissolved in corn oil (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... a small cohort of C57BL/6 mice were transferred to a diet of 0.2% (w/w) cuprizone powder (C9012, Sigma) in crushed mouse food pellets (Barrestock) ...
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bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich, cat# ...