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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and added dropwise to HEK293T cells that were seeded onto 10-cm dishes a day prior at a density of 5-6 × 106 cells in 10 ml of fresh DMEM with 10% heat inactivated FBS (Sigma-Aldrich #14009C). 18hrs after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... Digested pancreata were washed with HBSS +0.1% BSA and subjected to discontinuous density gradient using histopaque (6:5 Histopaque 1119:1077; Sigma, St. Louis, MO). The islet layer (found at the interface ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were incubated at 37 °C and 5% CO2 for 24 hrs and selected with growth medium containing 6 μg/mL puromycin (Sigma-Aldrich, P8833) and 750 μg/mL neomycin (Thermo ...
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bioRxiv - Bioengineering 2023Quote: ... The 1.5 ml resuspended RBCs were cultured in 50 ml HPS containing 2 mM CaCl2 and 2 µM calcium ionophore-4-bromo-A23187 (C7522, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in T75 flasks in a 5% CO2 incubator at 37°C for 16 h ...
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bioRxiv - Cancer Biology 2020Quote: Tetracycline-inducible CALM-AF10 mouse hematopoietic progenitor cells were cultured in 15% FBS DMEM medium without IL-6 and mCSF and treated with either 4 µg/mL doxycycline (DOX) (Millipore Sigma, Billerica, MA) or DMSO for 3 Days ...
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bioRxiv - Neuroscience 2019Quote: ... mice were gavaged at 4-6 weeks of age with five doses of 100 mg/kg of Tamoxifen (T5648, Sigma, St. Louis, MO) in corn oil (C8267 ...
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bioRxiv - Microbiology 2021Quote: Each pellet was resuspended in 500 μL (samples collected at 0 and 6 hr) or 650 μL (samples collected at 12 and 24 hr) with chilled (4°C) nuclease-free water (Sigma-Aldrich, Gillingham, UK). The resuspensions were frozen overnight at −80°C ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... the neurons of postnatal day 1–2 NMRI strain mice were grown 6 DIV on polyornithine-laminin (P3655 and CC095, Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141 ...
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bioRxiv - Microbiology 2020Quote: ... Tissues submerged in RNAlater solution were mixed with 2 ml of 6% (w/v) betaine (Sigma Aldrich, St. Louis, MO, USA) and placed on ice prior to crushing with mortar and pestle until gut homogenates were formed ...
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bioRxiv - Molecular Biology 2019Quote: ... tendon fascicles (n=6, independent samples from different mice) were stained with 2’,7’-Dichlorofluorescin diacetate (DCFH-DA, D6883, Sigma Aldrich). Control fascicles and cultured fascicles were incubated for 30 minutes with 50μM DCFH-DA in PBS at either 29°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1 × 107 PFU per ml of MNV-1.CW3 were incubated at temperatures ranging between 0 and 56°C for 2 or 6 h in the presence or absence of 500 μM bile acid (sodium glycochenodeoxycholate GCDCA, Sigma Aldrich) by enumeration by plaque assay.
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bioRxiv - Systems Biology 2020Quote: ... beads were recovered from the emulsion by washing with 2 × 30 mL 6 × saline sodium citrate buffer (diluted from Sigma #S6639) buffer in a 5 µm ÜberStrainer (pluriSelect) ...
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bioRxiv - Cell Biology 2022Quote: The neuroprotective effect of CA against 6-OHDA was evaluated by MTT reagent (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) (Sigma Aldrich, UK, M5655) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... Behaviour was monitored at 500 fps in 6 dpf larvae with their head restrained in 2% low-melting point agarose (Sigma-Aldrich) and their tail free to move ...
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bioRxiv - Genomics 2020Quote: ... The lentivirus containing the pLenti-FNLS-P2A-Puro or pLenti-ABERA-P2A-Puro or desired gRNAs were added to the 0.5 million of HUDEP-2 or HUDEP-2 stable cell line in a one well of the six-well plate along with 6 μg/ml Polybrene (Sigma-Aldrich) and 1% HEPES 1M buffer (Gibco ...
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bioRxiv - Physiology 2019Quote: Approximately 15 mg of the left TA muscle was homogenized using a glass tissue-homogenizer in 1.5 ml ice-cold lysis buffer (6 M Urea, 2 M Thiourea, 10 mM DTT, Complete Protease Inhibitor (1 tablet per 10 ml lysis, Sigma-Aldrich), PhosSTOP (2 tablet per 10 ml lysis ...
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bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently transduced twice with viral supernatant within 48 hrs in the presence of 50 IU/mL IL-2 and 6 mg/mL polybrene (Sigma-Aldrich). TCR-transduced T cells were expanded by stimulation with anti-CD3/CD28 Dynabeads (500,000 beads/106 cells ...
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bioRxiv - Developmental Biology 2021Quote: ... 5×104 cells were seeded onto ultra-low adherence 6-well culture plates in 2 mL of serum-free DMEM-F12 media (Sigma-Aldrich), supplemented with 10 µg/mL Putrescine ...
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bioRxiv - Cancer Biology 2022Quote: ... cellular suspensions were obtained from cultures and 1 x 106 million tumour cells were resuspended in 6 μL of 2% PVP (Polivinilpirrolidin, Sigma-Aldrich). Microinjection needles were crafted from borosilicate capillaries (1mm outer diameter x 0,58 mm inner diameter ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich) and 5 ng/ml FGF2 (Peprotech) ...
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bioRxiv - Pathology 2023Quote: ... and the whole brain of wildtype, heterozygous and homozygous mice (2 months, 6 months, 1 year) with phosphate buffer (PBS) containing 1% Triton-X100 (Sigma-Aldrich) and a Complete EDTA-free cocktail of protease inhibitors (Roche) ...
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bioRxiv - Immunology 2023Quote: ... virus supernatant was aspirated and preactivated PBMCs (0.5-0.8 x 106/well) in TexMACS medium supplemented with 100 IU/ml IL-2 and 6 ng/ml polybrene (Sigma-Aldrich) were added to each well ...
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bioRxiv - Plant Biology 2024Quote: Flowers at position 6 were gently incubated in staining solution (25 µM (2’,7’-dichlorodihydrofluorescein diacetate) (H2DCF-DA) (Sigma-Aldrich, D6883), 50 mM KCL ...
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bioRxiv - Physiology 2024Quote: ... subcutaneous white adipose tissue (sWAT) from male C57BL/6 mice was digested with 1 mg/mL type 2 collagenase (Sigma Aldrich) at 37°C for 40 minutes with shaking ...
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bioRxiv - Cancer Biology 2020Quote: ... and Hs 766T were plated at a density of 2×104 cells/cm2 and were treated for six days with 100 μM 5-aza-2’deoxycytidine (Sigma Aldrich, St. Louis, MO) or for 24 hours with 50 nM Trichostatin A (Sigma Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... The cell pellets were suspended in 5 ml pure water at 4 ℃ with 0.4 % TritonX-100 (Sigma-Aldrich), 2 μl DNAase Ⅰ(Sigma Aldrich ...
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bioRxiv - Bioengineering 2021Quote: Before staining with either 5-bromo-4-chloro-3′-indolyphosphate and nitro-blue tetrazolium (BCIP/NBT, Sigma-Aldrich) for alkaline phosphatase (ALP ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was pipetted from the pellet and 20 mM 5′-(4-Fluorosulfonylbenzoyl)adenosine hydrochloride (FSBA; Sigma Aldrich) was added to the supernatant ...
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bioRxiv - Genetics 2022Quote: ... ∼100 animals per condition were transferred to 4-5 NGM plates containing 7.5mM t-booh (Luperox TBH70X, Sigma). Worms were scored for survival every 2 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were developed using nitroblue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP) (Sigma) in staining buffer (100 mM Tris pH9.5 ...
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bioRxiv - Neuroscience 2019Quote: ... On day 4 after plating cortical neurons were treated with 5 µM Cytosine D-Arabino-Furanoside (Sigma-Aldrich) in order to prevent proliferation of non-neuronal cells.
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bioRxiv - Genetics 2021Quote: ... After blocking with 4– 5% skim milk (MBcell, South Korea) in TBST including 25 mM Tris (Sigma, USA), 140 mM NaCl (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Primary antibodies were applied overnight at 4°C in a blocking buffer of 5% donkey serum (Sigma-Aldrich) at the following concentrations ...
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bioRxiv - Developmental Biology 2021Quote: ... and color was developed with 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT; Sigma). Following color development ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... washed with PBS and incubated for 4 hours in complete DMEM/F12 containing 5 µg/ml cycloheximide (Sigma) to allow receptor recovery and prevent de novo protein synthesis18 ...
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bioRxiv - Molecular Biology 2022Quote: U2OS cultured at 37 °C/5% CO2 in 15 cm2 dishes supplemented with 4 μg/ml Doxycycline (Sigma) for 48 hours to induce exogenous SAE1/SAE2 expression ...
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bioRxiv - Molecular Biology 2022Quote: ... Bands were stained in 5-bromo-4-chloro-3-indolyl-phosphate and nitro blue tetrazolium solution (Sigma-Aldrich). The membrane image was acquired using a digital steel camera EOS M6 mark II with a lens EF16-35 mm F4L IS USM (Canon Inc. ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed using 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) (Sigma Aldrich, 11697471001 ...
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bioRxiv - Microbiology 2022Quote: ... RNA (5 μg) was enzymatically hydrolyzed for 4 h at 37 °C with benzonase (99% purity, Novagen 70664), bacterial phosphatase (ThermoFisher 18011015 ...
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bioRxiv - Cell Biology 2023Quote: ... skin samples were digested at 4°C overnight in 5 mg/mL dispase II solution (#D4693 Sigma-Aldrich) in HBSS (#H8264 Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with an Amicon Ultra-4 centrifugal filter with a 5 kDa molecular weight cutoff (UFC8003, Merck Millipore), snap frozen and stored at -80 °C either directly (used for cryo-EM ...
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bioRxiv - Genetics 2023Quote: ... The synthesized sgRNAs were extracted with phenol (pH 4–5):chloroform:isoamyl alcohol (125:24:1) (Sigma, Cat#P77619), precipitated with isopropanol ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then bleached overnight at 4 C in methanol containing a 5% of hydrogen peroxide (Sigma-Aldrich). Rehydration was done by incubating the samples in methanol 60% ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then bleached overnight at 4 C in methanol containing a 5% of hydrogen peroxide (Sigma-Aldrich). Rehydration was done by incubating the samples in methanol 60% ...
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bioRxiv - Cell Biology 2024Quote: ... falciparum cultures (4-8 hpi) were treated with apicoplast inhibitors (either 5 µM clindamycin (Sigma-Aldrich, PHR 1159) for > 50 times the delayed death IC₅₀ ...
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bioRxiv - Immunology 2023Quote: ... samples were cut into thirds and incubated overnight at 4°C in 5 mg/mL Dispase II (Sigma) in Hanks Buffered Salt Solution (HBSS) ...
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bioRxiv - Developmental Biology 2023Quote: ... pregnant Sprague-Dawley rats received 100mg of nitrofen (2,4-Dichlorophenyl 4-nitrophenyl ether; Sigma CAS#1836-75-5) in 1mL of olive oil at E9.5 to induce fetal pulmonary hypoplasia ...
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bioRxiv - Microbiology 2024Quote: ... and 12,000 × g for 5 minutes at 4°C using a Sigma 3K30 centrifuge (Sigma Laborzentrifugen GmbH, Germany), and the supernatant was collected ...
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bioRxiv - Cancer Biology 2021Quote: ... a sterile β-mercaptoethanol solution was prepared (5 μl of 2-mercaptoethanol (Sigma-Aldrich, Munich, Germany)) in 8.45 ml of PBS ...