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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: Thoraces from 2–3-day-old adult Drosophila were dissected in 4% paraformaldehyde (PF, Sigma))) ...
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bioRxiv - Cell Biology 2023Quote: ... fixed for 2 h in formaldehyde solution 4% buffered to pH 6.9 (Sigma-Aldrich, #100496), and analyzed for morphogenetic defects ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-ura / SC-ura + 50µg/ml 6-azauracil (6-AU, Sigma), SC-leu / SC-leu + 0.4mM CuSO4 (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... 6-hydoxydopamine (6-OHDA; cat. #H4381; Sigma-Aldrich, St. Louis, MO) was dissolved in vehicle solution (0.1 % ascorbic acid [cat ...
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bioRxiv - Physiology 2021Quote: ... and IL-6 (KIT ELISA IL-6-RAB0308-1KT, Sigma-Aldrich) in BALF following the kit instructions ...
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bioRxiv - Microbiology 2022Quote: ... The forward primer contained a 6-FAM (6-carboxyfluorescein) tag (Sigma). EMSA reaction mixtures (25 μL volume ...
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bioRxiv - Biophysics 2023Quote: ... Both resiniferatoxin (Alomone Labs) and 6’-iodoresiniferatoxin (6’-iRTX, Sigma-Aldrich) were dissolved in ethanol to create a 1 mM stock and subsequently diluted to a 200 nM working concentration with the bath solution ...
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bioRxiv - Neuroscience 2024Quote: For the intranasal administration of 6-hydroxydopamine (6-OHDA; Sigma-Aldrich), MMTV-PyMT +/-female mice at 1 week after the tumor onset were briefly anesthetized with 3% isoflurane ...
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bioRxiv - Cell Biology 2024Quote: ... cells were selected with 6 µg/mL 6-thioguanine (Sigma-Aldrich). The generated mutant cells were stocked in CELLBANKER 1 (Nippon Zenyaku Kogyo).
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bioRxiv - Molecular Biology 2022Quote: ... The FahPM/PM mice were kept on water supplemented with 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. #: PHR1731-1G). Mice with more than 20% weight loss were humanely euthanized according to IACUC guidelines ...
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bioRxiv - Microbiology 2021Quote: ... individuals were placed in a microtube containing in 100 µl of either Schneider’s media (Experimental Replicates 1 and 2) or RPMI media (Experimental Replicates 3 and 4) to which 2% Penicillin/Streptomycin and Amphotericin B (Sigma-Aldrich, Dorset, UK) had been added ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 months old mice were orally gavaged with 3 x 2 (Mist1creERT/+KRASG12D/+) or 5 x 5 mg (Ptf1acreERT/+KRASG12D/+) of tamoxifen (TX; Sigma-Aldrich, T5648-5G) at Western University and 5 x 4 mg (Ela-creERT ...
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bioRxiv - Immunology 2020Quote: Intracellular cytokine staining was performed on spleen and LN single cell suspensions following 5-6 hr stimulation with PMA (10ng/ml) (Sigma Aldrich) and ionomycin (1μg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... and mice exposed to 5 days of photo-oxidative damage (PD) (n=6) were lysed in CellLyticTM Cell Lysis Buffer (Sigma-Aldrich) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: HBP inhibition in vivo was achieved by treatment of tumor bearing mice with 3 doses (10 mg/kg) of DON (6-Diazo-5-oxo-L-norleucine, Sigma-Aldrich) every 3 days ...
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bioRxiv - Immunology 2022Quote: Lysates from cells treated in 6-well plate were heated for 5 minutes at 100 ºC with the same volume of Leammli Buffer 2X (Sigma-Aldrich). Samples were separated in a vertical electrophoresis on 12% polyacrylamide gels (Biorad ...
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bioRxiv - Immunology 2020Quote: ... Sorted cells were incubated with slices (5×105 cells/slice) for 6 hours in Hanks’ Balanced Salt solution (Sigma-Aldrich, USA) supplemented with 1% of penicillin/streptomycin solution (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells were resuspended in standard growth medium without antibiotics and plated in 6-well culture plates (5 × 104 cells/well) after mixing with 10 µg/ml polybrene (EMD Millipore) and lentivirus supernatant ...
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bioRxiv - Neuroscience 2022Quote: ... CAS# 614-96-0), 6-methylindole (97%, CAS# 3420-02-8) and 7-methylindole (97%, CAS# 933-67-5) were acquired from Sigma-Aldrich, and 4-methylindole (99% ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μl PCR product for each barcoded sample was mixed with 5 μl 6×Orange G DNA Loading Dye (Orange G, 6X, Sigma, Germany) and run on a 1 % agarose gel in TAE 1X buffer ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and the pellet resuspended in 1 ml of BHI containing 10 µM 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFDA-SE, Sigma #21888). The suspension was incubated on a rotating shaker at 37°C and 200 rpm for 45 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was suspended in 10 ml of Histopaque 1100 (5 ml histopaque 1077; Sigma 10771 and 6 ml histopaque 1119; Sigma 11191), centrifuged for 20 minutes at 1500 rpm at 4°C and the pellet washed 2 times with QB ...
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bioRxiv - Neuroscience 2022Quote: ... After the addition of the appropriate amount of the 6× Laemmli Sample Buffer (Bioland scientific LLC, sab03-02) with 5% ß-mercaptoethanol (Sigma) protein samples (10-30 μg ...
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bioRxiv - Biophysics 2023Quote: ... The remaining exposed surface was blocked for 5 minutes with 6% Pluronic F-127 (w/v) (Sigma P2443. MW: 12.5 kDa) and 15mg/ml of Bovine Serum Albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: EMSAs for the SAP-domain were performed with DNA such that the single strand or one of the two strands in dsDNA was 5’-labeled with fluorescein (6-FAM, Sigma-Aldrich) (Table 1) ...
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bioRxiv - Plant Biology 2023Quote: ... and supernatant was mixed with 6 μL loading buffer (750 mM ε-aminocaproic acid and 5% (w/v) pure Coomassie Brilliant Blue G (B0770, Sigma)) and loaded onto a 6-12% gradient polyacrylamide gel ...
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bioRxiv - Microbiology 2023Quote: ... For determination of viable population using viability dyes, diluted suspensions were incubated in the dark either with 5(6)-carboxyfluorescein diacetate (CFDA, 30 µM) (Sigma-Aldrich) for 30 min or with a mix of SYTO 9 (3.34 µM ...
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bioRxiv - Biochemistry 2024Quote: ... mice were fasted for 6 hours (for GTT) or 5 hours (for ITT) and received an intraperitoneal injection of glucose (1g/kg, Sigma Aldrich) or insulin (0.75 units/kg ...
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bioRxiv - Genetics 2022Quote: Animals were raised and maintained from embryonic stages onward on normal food supplemented with 3OH-K (1mg/ml of 3-Hydroxy-DL-kynurenine, H1771, Sigma Aldrich; dissolved in DMSO) or KYNA (1mg/ml of Kynurenic acid ...
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bioRxiv - Neuroscience 2024Quote: 0–1 h old larvae were fed yeast paste (sham-controls) or yeast paste containing 100 mg/mL Hydroxy Urea (HU, Sigma Aldrich, St Louis, MO) for 4 h via standard protocol (41 ...
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bioRxiv - Neuroscience 2022Quote: ... and the supernatant was gently layered on a discontinuous Percoll gradient (2 %, 6 %, 10 %, and 20 % v/v in Tris-buffered 0.32 M sucrose; Sigma-Aldrich). After centrifugation at 33’500 g for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was titrated into 2 × 105 PC-3M cells on 6-well plates with 2 mL complete RPMI-1640 supplemented with 8 μg/mL polybrene (Sigma). After overnight infection ...
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bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... pH 7.4) containing 10 mM sodium azide and 6 mM 2-deoxy-D-glucose (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 50 min ...
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bioRxiv - Neuroscience 2020Quote: ... 6-month-old littermate matched were injected subcutaneously with vehicle [40% (w/v) (2-hydroxypropyl)-β-cyclodextrin (Sigma– Aldrich #332607)] or MLi-2 dissolved in vehicle at a 30 mg/kg final dose ...
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bioRxiv - Cell Biology 2021Quote: ... G6P concentrations were determined at 2 hrs and 6 hrs post activation and compared to unactivated controls using a G6P kit (Sigma) and following manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... Cross-links were reversed by incubating the eluted samples and inputs at 42°C for 2 hours and 67°C for 6 hours in the presence of Pronase (Sigma) and TE buffer ...
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bioRxiv - Neuroscience 2021Quote: ... the samples were mounted in polyvinyl alcohol-based mounting medium containing 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; FUJIFILM-Wako) and 2.5% (w/v) 1,4-diazabicyclo[2.2.2]octane (Sigma, St. Louis, MO, USA) as an antifade ...
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bioRxiv - Genetics 2022Quote: ... Slides were then rinsed in washing buffer five times for 3 min each and nuclei were stained with 4lr,6-diamidino-2-phenylindole (DAPI, cat # D-1388, Sigma) at a concentration of 1 μg/ml for 10 min at RT ...
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bioRxiv - Immunology 2019Quote: PBMCs (freshly isolated and previously frozen) and fresh whole blood were each stimulated for either 2 or 6 h with (1) PMA/ionomycin (Sigma) (PBMC ...
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bioRxiv - Developmental Biology 2022Quote: ... 2/3 mTeSR1 + 1/3 N2 medium + LSX + PSD Day 6,7: 1/3 mTeSR1 + 2/3 N2 medium + PSD At day 6 cells were detached by Accutase solution (Sigma-Aldrich) and incubated at 37°C for 20 minutes to obtain a single cell suspension ...
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bioRxiv - Synthetic Biology 2023Quote: ... All samples diluted with buffer to the final concentration of 2 μM Aβ were supplemented with 6 μM ThT (Sigma), with a range of concentrations of the binders per experiment ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of acid ninhydrin (glacial acetic acid, 6 M phosphoric acid, ninhydrin (2,2-dihydroxyindane-1,3-dione, Sigma, ON, Canada) were boiled in a water bath at 100 °C for 1 hr ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... mice were fasted for 6 h and received an oral (2 g/kg body weight) glucose load (G7021, Sigma Aldrich). For the insulin tolerance test ...
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bioRxiv - Biophysics 2023Quote: ... 1.5 – 2 million cells were plated on a 6-well-plate coated with 10 µg/ml fibronectin (Sigma; F1141-5MG) and maintained in IMDM (GlutaMAX ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Microbiology 2024Quote: ... The pesticides were: 3,6-dichloropyridine-2-carboxylic acid (i e. clopyralid, pesticide analytical standard, CAS 1702-17-6, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Neuroscience 2020Quote: Focal white matter demyelinating lesions were induced in the cerebral striatum of 12-week-old male C57BL/6 mice by stereotaxic injection of 4 μL of 0.01% (v/v) ethidium bromide (Sigma-Aldrich, Shanghai, China) using a Hamilton syringe ...
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bioRxiv - Cancer Biology 2019Quote: ... 107 cells cultured with 100nM 4-OHT or ethanol control for 6 or 24 hours were incubated with 1% formaldehyde (Sigma-Aldrich, F8775) at room temperature for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... were pooled together (6 mL) and concentrated/buffer exchanged using an Amicon® Ultra-4 Centrifugal Filter Units (3 kDa, Millipore, Ireland). The final volume of the purified bacteriocin protein fraction was reduced to 200 μL ...