Labshake search
Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... The cells were then incubated overnight at 4°C with FLAG antibody (1:500, F1804, Sigma), and incubated with TRITC-conjugated secondary antibody (1:200 ...
-
bioRxiv - Cell Biology 2022Quote: ... and probed at 4 ºC overnight with mouse monoclonal anti-FLAG M2 (1:1000, Sigma-Aldrich) and mouse monoclonal anti-α-tubulin B512 (1:5000 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 5.0 mM HEPES pH 7.6) diluted in 10,000 MW rhodamine-dextran solution (1:4; Sigma-Aldrich). The mixed solution was injected again into the KV and only embryos with rhodamine-positive KV’s were selected for the experiment ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated overnight at 4°C with primary antibodies: puromycin (mouse, 1:5000, Millipore), rRNA (mouse ...
-
bioRxiv - Neuroscience 2023Quote: ... and then incubated overnight at 4°C with 1:000 rabbit anti-laminin (Sigma-Aldrich L9393). Sections were washed 3 times with PBS and then incubated with 1:1000 goat anti-rabbit IgG Alexa-Fluor 488 (Invitrogen ...
-
bioRxiv - Biophysics 2023Quote: ... in BRB80 buffer (80 mM Pipes, 1 mM EGTA, 4 mM MgCl2, pH 6.8, Sigma-Aldrich) for 1 min ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were diluted 1:4 in sterile phosphate buffered saline (PBS, pH 7.0, Sigma-Aldrich) and filtered (pore size 20-22 µm ...
-
bioRxiv - Genomics 2023Quote: ... and incubated with primary antibody against TMEM251 (HPA048559, Sigma-Aldrich; 1:200 overnight at 4°C) followed by Alexa Fluor 488-conjugated secondary antibody (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... zymosan (life technologies, added at a 1:5 ratio) or R848 (Sigma-Aldrich; 4 μg/ml). After this time ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated overnight at 4°C with mouse anti-acetylated tubulin (1:20,000; Sigma, T7451) and mouse anti-α-tubulin (1:10,000 ...
-
bioRxiv - Biochemistry 2023Quote: ... diluted to 4 ml and passed over a 1 ml column of DOWEX 1X8 (Sigma Aldrich). Dowex column flow through was lyophilized and applied to a Sephadex G10 column (Cytiva ...
-
bioRxiv - Bioengineering 2023Quote: ... 1 µL of matrix solution composed of saturated α-cyano-4-hydroxycinnamic acid (Sigma, Lyon, France), 50% (v/v ...
-
bioRxiv - Neuroscience 2023Quote: ... and the postsynaptic AMPA receptors (Mouse IgG2B anti PAN-GluA1-4, 1:500; Millipore-Sigma: #MABN832). Coverslips were incubated with primary antibodies overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... and the postsynaptic AMPA receptors (Mouse IgG2B anti PAN-GluA1-4, 1:500; Millipore-Sigma: #MABN832). Coverslips were incubated with primary antibodies overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated over 72 h at 4°C with mouse anti-NeuN (1:300, Millipore, MAB377), rabbit anti-pS6S235/236 (1:300 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The reaction was stopped by the addition of 1 ml of methimazole (MMI; 4 mM, Sigma). After two washes with cold PBS ...
-
bioRxiv - Microbiology 2023Quote: ... and CF568-conjugated donkey anti-sheep IgG (Sigma-Aldrich, SAB4600078-50UL, 1:500, 4 µg/mL).
-
bioRxiv - Immunology 2023Quote: ... 1 x 15 minutes of recording with DMPP (1,1-Dimethyl-4-phenylpiperazinium iodide, Sigma-Aldrich D5891) application and 1 x 15 minutes of washout recording ...
-
bioRxiv - Physiology 2024Quote: ... Sections were incubated with the primary antibody at 4℃ overnight (TMEM16a (Millipore Sigma; P6593; 1:250), Na+/K+ ATPase (Abcam ...
-
bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies (see Table S11) were incubated overnight at 4°C in 1% BSA (Sigma, A2153), then washed 3 times ...
-
bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
-
bioRxiv - Bioengineering 2022Quote: Specimens were permeabilized with 4% paraformaldehyde and incubated for 1 hour at room temperature in primary antibody anti-vinculin (1:500, Sigma Aldrich, V4505), secondary antibody Alexa Fluor 488 (1:600 ...
-
bioRxiv - Neuroscience 2021Quote: ... Slides were incubated overnight at 4°C with primary antibodies, GFP (Novus NB1001770, 1:100) and NeuN (EMD Millipore MAB377, 1:150) in 0.1% Triton X-100 ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4 °C with primary antibodies (GSX2: rabbit 1:200, Merck-Millipore ABN162; PAX6 mouse 1:80, DSHB) diluted in solution containing 2.5% NGS and 0.02% Triton X-100 ...
-
bioRxiv - Neuroscience 2021Quote: ... the cells were fixed with 4% PFA and immunostained with mouse anti-HA (Covance, 1:1000) or rabbit anti-Flag (Sigma, 1:1000), followed by incubation with AlexFluor-647-conjugated anti-human Fc γ and species-specific AlexaFluor-488- or -546-conjugated secondary antibodies ...
-
bioRxiv - Developmental Biology 2019Quote: ... Tissues were blocked in 5% normal donkey serum before an overnight incubation with the primary antibody at 4°C (1:200 for ICSBP antibody, sc-365042, Santa Cruz; and 1:100 for MEF2C antibody, SAB4501860, Sigma-Aldrich). The slides were washed twice in PBS at room temperature and applied with secondary antibody diluted 1:200 in 1% BSA prepared in PBS (biotinylated anti-mouse IgG (H+L) ...
-
bioRxiv - Cancer Biology 2020Quote: ... were incubated for 1 h at RT and after washing coverslips were incubated for 4 min with DAPI (1:4000, Sigma-Aldrich #D8417) and mounted with ProLong Gold Antifade reagent (Invitrogen #P10144).
-
bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... and applied on previously seeded NIH3T3 Flp-In cells at a ratio of 1:1 (vol/vol, supernatant : NIH3T3 growth medium) together with 4 µg/ml Polybrene® (Sigma-Aldrich). Transduced cells were cultured in regular growth medium supplemented with 5 µg/ml blasticidin (Carl Roth GmbH + Co ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were harvested on ice and lysed by rotation for 1 h at 4°C in JIES buffer (see above) containing 1× SIGMAFAST protease inhibitor cocktail (Sigma-Aldrich, S8830). Subsequently ...
-
bioRxiv - Physiology 2023Quote: ... Membranes were blocked in 1% Casein/PBS (1 hour, room temp) and incubated with the following primary antibodies (4°C, overnight): anti-Nup93 (1:1000, Sigma-Aldrich, HPA017937); anti-Nup107 (1:200 ...
-
bioRxiv - Immunology 2023Quote: ... Membranes were blocked in TBS-T with 5 % non – fat milk for 1 hour in RT following incubation overnight at 4 °C with primary (1:1000) antibodies: Flag (Sigma, Cat. F1804), IL-1β (Cell Signaling Technology ...
-
bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
-
Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... HEK293T cells were seeded in a 6-well plate so that they were 70-90% confluent the next day (∼0.8x10^6 - 1x10^6 cells/well in 2 mL of standard growth media containing 10% FBS (Millipore, TMS-013-B) and 1x Penicillin-Streptomycin (Invitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were then seeded in 6-well plates and cultured for 7 to 14 days in the presence of 60 μM 6-thioguanine (Sigma-Aldrich, A4882) [23] ...
-
bioRxiv - Genetics 2024Quote: ... germinal vesicle-intact oocytes were collected from 6 week to 6 month-old female mice in M2 media (Sigma-Aldrich, Cat# M7167). Oocytes were then kept in a 60 x15 mm polystyrene petri dish (Falcon ...
-
bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
-
bioRxiv - Plant Biology 2019Quote: ... and 0.5 mg/mL 5(6)-Carboxyfluorescein diacetate (Sigma) was applied to the cut using a P2 pipette ...
-
bioRxiv - Biophysics 2021Quote: ... D-Glucose 6-phosphate sodium salt (G6P; Sigma-Aldrich), or Adenosine 5’-diphosphate sodium salt (ADP ...
-
bioRxiv - Biophysics 2021Quote: ... and 5(6)-carboxyfluorescein were purchased from Sigma-Aldrich. Methanol (LC/MS grade ...
-
bioRxiv - Biophysics 2020Quote: ... The equal volume of 1,6-Hexanediol (Sigma, 240117, 6%) in no-serum RPMI was added on the petri dish and incubated for 30 seconds ...
-
bioRxiv - Cell Biology 2020Quote: 6-ketocholestanol and phloretin (Sigma-Aldrich, St. Louis, MO) were used for measuring penetratin uptake at increased and decreased dipole potentials ...
-
bioRxiv - Cell Biology 2020Quote: ... and 2U of glucose-6 phosphate dehydrogenase (Sigma G6378) were added ...
-
bioRxiv - Cell Biology 2020Quote: ... 2% OsO4 and 1.5% K4Fe(CN)6 (Sigma-Aldrich). Fixed cells were then stained en bloc in 2% aqueous uranyl acetate ...
-
bioRxiv - Neuroscience 2021Quote: ... Capsaicin (8-Methyl-N-vanillyl-trans-6-nonenamide, Sigma), RQ00203078 (Tocris ...
-
bioRxiv - Molecular Biology 2021Quote: ... 150 μM of ferrous ammonium citrate (Sigma, 22896-6) or iron chelator IV-21H7 (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: Assemble RNA denaturation mix: 6 ml DMSO (Sigma 34869), 2 ml 40% glyoxal solution (Sigma 50649 ...
-
bioRxiv - Developmental Biology 2019Quote: ... and 6-BIO (0.2 µM, Sigma-Aldrich, cat. #B1686) compounds or the same amount of DMSO (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2019Quote: ... 6-diamidino-2-phenylindole (DAPI) was bought from Sigma. Co-localization of SOX2 and OCT4 was analyzed using a Leica TCS SP5 confocal microscope (Leica TCS SP5 ...
-
bioRxiv - Biochemistry 2019Quote: ... and 6 µL/mL protease inhibitors cocktail (Sigma P8340), 24 U/mL benzonase nuclease ...