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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The incubation was performed in the presence and absence of an NADPH generating system: 1 mM glucose-6-phosphate (Sigma Aldrich) 0.1 mM NADP+ (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... cellular suspensions were obtained from cultures and 1 x 106 million tumour cells were resuspended in 6 μL of 2% PVP (Polivinilpirrolidin, Sigma-Aldrich). Microinjection needles were crafted from borosilicate capillaries (1mm outer diameter x 0,58 mm inner diameter ...
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bioRxiv - Immunology 2023Quote: ... Macrophages were generated by culturing THP-1 (6×105/ml) cells with 15 nM phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) in the media ...
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bioRxiv - Physiology 2023Quote: ... Cells were then washed once with warm Tyrode salts solution and incubated with 10−6 calcein-AM in 200uL warm Lysis buffer (1% Triton X-100 (Sigma #X100) in HBSS ...
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bioRxiv - Cell Biology 2023Quote: ... full differentiation of the organoids was achieved by a concomitant treatment for 6 days with 1 mM valproic acid (P4543, Sigma-Aldrich), with the addition of 2.5 mg/mL inhibitor of WNT production-2 (IWP-2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Gold fiducials (6 nm; BSA tracer; Aurion, Wageningen, Netherlands) were buffer exchanged into 40 g L-1 sea salts (Sigma Aldrich) using centrifugal ultrafiltration unit (100 kDa MWCO ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1 million cells per 6-well plate (or 375k cells per 12-well plate) in poly-L-ornithine (Sigma P3655) -coated wells in Neuron Differentiation Media with 10 uM ROCK inhibitor ...
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bioRxiv - Genomics 2023Quote: ... with the lentivirus at different dilutions in 1-ml F12k media (ATCC 30-2004) in the presence of 6 ug/ml polybrene (Sigma H9268). After selection with 1.5 ug/ml puromycin (ThermoFisher AAJ672368EQ ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biophysics 2023Quote: Mechanically passaged hiPSCS were plated on 10 µg/mL Vitronectin XF in 6 well TC plates in 1 μM all-trans-RA (Cat. No. R2500-25MG, Sigma-Aldrich) to NutriStem® hPSC XF Medium (Growth Factor-Free)(Cat ...
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bioRxiv - Physiology 2024Quote: ... subcutaneous white adipose tissue (sWAT) from male C57BL/6 mice was digested with 1 mg/mL type 2 collagenase (Sigma Aldrich) at 37°C for 40 minutes with shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with following primary antibodies at 4 °C overnight: TRA-1-60 (Merck Millipore), OCT3/4 (H-134 ...
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bioRxiv - Microbiology 2021Quote: ... The blocks were infiltrated with a 4:1 mixture of propylene oxide (PO):Durcupan resin (Sigma) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... at 4°C followed by a contrasting step with 1% thiocarbohydrazide (Sigma-Aldrich, St. Louis, USA) for 1 h at room temperature and 1.5 h incubation with 2% OsO4 ...
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bioRxiv - Neuroscience 2019Quote: ... All cells used in this study were exposed to 1 µM 4-hydroxytamoxifen (Sigma, Cat#: H6278) for 24 hours prior to studies of Ucp2 function.
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bioRxiv - Immunology 2019Quote: ... 4°C for 5 min and washed once with a PBS containing 1% BSA (Sigma Aldrich). The single cell suspensions used for 10X Genomics scRNAseq analysis were cleared of red blood cells by using a Red blood cell lysis buffer (eBioscience ...
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bioRxiv - Neuroscience 2019Quote: ... All cells used in this study were exposed to 1 µM 4-hydroxytamoxifen (Sigma, Cat#: H6278) for 24 hours prior to studies of Ucp2 function.
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bioRxiv - Developmental Biology 2019Quote: ... Sections were incubated overnight at 4°C with monoclonal mouse anti-NG2 (Sigma, C8035, 1:100), mouse anti-SOX2 (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... for 1 hour at 4°C prior to permeabilization with 0.1% triton X-100 (Sigma-Aldrich) and incubation in blocking buffer containing 10% goat serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: Amoxicillin trihydrate: potassium clavulanate (4:1) (AMC) was diluted in molecular grade water (both Sigma, UK) to a stock concentration of 1 mg/ml and filter sterilised through a 0.22 μM polyethersulfone filter unit (Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... was added to the medium at 4 mM together with 1% Igepal CA-630 (Sigma-Aldrich). Cells were stained with 0.5 μg/ml BODIPY 493/503 (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... Guts were stained over night at 4°C with Phalloidin-Atto-550 (1:3000; Sigma-Aldrich), washed three times with PBS and embedded in Vectashield (Vector Laboratories).
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated overnight at 4 °C with the polyclonal antibody ATB0,+ (1:100, Sigma-Aldrich), then washed with PBS and further incubated for 45 min with Alexa Fluor 488 antibody (1:400 ...
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bioRxiv - Cancer Biology 2020Quote: ... All N/Tert-1 cells were also supplemented with 4 μg/ml hygromycin B (Millipore Sigma). All cells not directly purchased from providers were cell type confirmed by Johns Hopkins or MD Anderson cell line authentication services ...
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bioRxiv - Microbiology 2020Quote: ... 1 µL of matrix solution composed of saturated α-cyano-4-hydroxycinnamic acid (Sigma, Lyon, France), 50% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in 1.5% BSA for 1 hr at RT and incubated with 4% PFA (Sigma) for another 24 hr in case of SARS-CoV-2 infected cells ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF) and 1x PhosSTOP phosphatase inhibitor cocktail (Sigma-Aldrich), and centrifuged at 10,000 x g for 10 minutes at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... on 25 × 75 × 1 mm glass slides using Mowoil 4-88 mounting medium (Sigma-Aldrich: 81381). Mounting medium was set at 4°C and slides were imaged within 1 hour.
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C with rabbit anti-tyrosine hydroxylase (1:1000 Millipore, USA) in PBS-T and 2% NDS solution ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were incubated for 1 hour at room temperature with 4% BSA (Millipore Sigma, #82-067) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight at 4 °C in guinea pig anti-VGLUT1 (1:1000, Millipore AB5905) or guinea pig anti-VGLUT2 (1:8000 ...
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bioRxiv - Cancer Biology 2021Quote: ... SHP2i was diluted in 50 mM Sodium Citrate Buffer pH = 4 with 1% Hydroxyethylcellulose (Sigma-Aldrich), 0.25% Tween (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were subsequently incubated overnight at 4°C with primary antibodies (S100β 1/200, Sigma S2532 ...
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bioRxiv - Neuroscience 2021Quote: Colonies at selected phases (Tables 1-4) were anaesthetized with MS222 (tricaine methanesulfonate, Sigma, A5040-25) and fixed in 4% PFA in MOPS (0,1 M MOPS ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated at 4 °C overnight with primary antibodies against APP (1:1000, Millipore) or APP-CTFs (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4°C with primary antibodies against GAD-67 (Millipore, 1:2000) and parvalbumin (Swant ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4°C with goat anti-ChAT (1:100, Millipore Sigma-Aldrich, AB144P) and anti-STMN2 (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... The blocks were infiltrated with 4:1 mixture of propylene oxide (PO):Durcupan resin (Sigma Aldrich) for 1 hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μM Na3VO4) with 1 mM dithiothreitol (DTT) and 4 μg/ml leupeptin (Sigma Aldrich;L2884). Lysates were extracted by further addition of 800 μL of NP-40 buffer ...
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bioRxiv - Cell Biology 2020Quote: ... with 1 μg/mL 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) protease inhibitor (Sigma-Aldrich, Inc.) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2020Quote: ... and reaction overnight at 4 °C with mouse anti-MAP2 (1:2,000; Sigma-Aldrich, #M4403, RRID:AB_477193) and then mouse anti-GAPDH (1:10,000 ...
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bioRxiv - Biochemistry 2019Quote: ... 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5’-monophosphate (ZMP; Sigma Aldrich, St. Louis, MO) at a stock concentration of 50 mM in dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2020Quote: SNAP-5114 (1-[2-[tris(4-methoxyphenyl)methoxy]ethyl]-(S)-3-piperidinecarboxylic acid) from Sigma-Aldrich
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bioRxiv - Molecular Biology 2021Quote: ... Insoluble aggregates were removed by centrifugation (20,000 x g, 30 min, 4°C; Sigma 1-16K). Solubilized membrane proteins (1 mL ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated overnight at 4 °C with primary antibodies to mouse Foxp4 (Millipore, #ABE74, 1:100), UCP1 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy5-conjugated secondary antibodies (1:200; AP192SA6; Merck Millipore, USA; 17 hrs at 4°C). Similar immunolabeling steps were followed for the subsequent sequential staining ...
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bioRxiv - Cell Biology 2019Quote: ... media was replaced with complete growth media supplemented with 4 μg mL-1 polybrene (Sigma-Aldrich) and 100 µL of the respective retroviruses were added dropwise to the wells ...
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bioRxiv - Cell Biology 2021Quote: ... paraformaldehyde (PFA) and 4-(2-hydroxyethyl)-1-piperazine-ethane-sulfonic acid (HEPES) were purchased from Sigma Chemical (St ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were incubated overnight (4 °C) with mouse anti-NeuN (1:500; Millipore, MAB377), rabbit anti-GFP (1:1000 ...