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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with primary antibodies overnight at 4°C (TNRC6A, human index serum, 1:3000; FOXJ1, eBioscience, # 14-9965-82, 1:100; Ac-α-tub, Sigma, # T7451 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt, Germany; SOX9, 1:1000, #AB5535 EMD Millipore, Burlington ...
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bioRxiv - Bioengineering 2023Quote: ... pH 14), hydrogen chloride solution (c(HCl)=1, pH 1), and buffer solution (pH 4, pH 9) were purchased from Sigma-Aldrich. Methacryloxyethyl thiocarbamoyl rhodamine B (MA-RhB ...
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bioRxiv - Genetics 2024Quote: ... Membranes were incubated overnight at 4°C in primary antibodies (Flag, F7425, Sigma-Aldrich, 1:1000; HDAC2, ab32117 Abcam, 1:1000; acetylated Tubulin, T6793 Sigma, 1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Neuroscience 2021Quote: ... the electrode surfaces of 6-well Axion plates (Axion Biosystems, CytoView MEA 6) were coated with 10 mg/mL poly-D-lysine (Sigma, P7280) at room temperature overnight ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were resuspended in 6 mL of cold water and lysed by adding them dropwise to 6 mL of boiling 8% SDS (Sigma-Aldrich) within 10 min ...
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bioRxiv - Microbiology 2022Quote: ... the msfGFP levels were normalized for OD600 and converted to absolute units using 5(6)-carboxyfluorescein (5(6)-FAM) (Sigma Aldrich) as a calibrant (36 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on Greiner Bio-One CELLSTAR Polystyrene 6-well Cell Culture Multiwell Plates coated first with Poly-L-ornithine hydrobromide (6 μg/mL in 1xPBS (Sigma-Aldrich), 1 h at 37℃ ...
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bioRxiv - Microbiology 2023Quote: ... The relative msfGFP measurements were normalised for their respective OD600 values and subsequently converted to absolute units of the calibrant 5(6)-carboxyfluorescein (5(6)-FAM)) (Sigma-Aldrich). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... the tamoxifen active metabolite 4-hydroxytamoxifen (4-OHT, Sigma, cat#H6278) was used instead of tamoxifen ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently fixed overnight at 4°C in 4% paraformaldehyde (Sigma). Next ...
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bioRxiv - Biophysics 2021Quote: ... 4 cm by 4 cm in chloroform (Sigma Aldrich, analytical standard) and placed the piece of paper inside the plastic container with the recorded fungal substrate.
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 1μM 4-hydroxy tamoxifen (4-OHT) (Sigma) for 48h ...
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bioRxiv - Neuroscience 2020Quote: ... QX314 chloride and 4-aminopyridine (4-AP) were from Sigma-Aldrich, (St ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were induced using 4-Hydroxytamoxifen (4-OHT) (H7904; Sigma H7904) from fresh or pre-heated (65°C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 3 days post-infection 4 mL of paraformaldehyde 4% (#158127, Sigma) was added and incubated overnight at 4ºC ...
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bioRxiv - Immunology 2023Quote: ... 100 μM 4-aminobenzoic acid hydrazide (4-ABAH, Sigma-Aldrich, A41909) or 20 μM disulfiram (Tocris ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were fixed overnight at 4°C in 4% paraformaldehyde (Sigma) in PBST ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were fixed overnight at 4°C in 4% paraformaldehyde (Sigma) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 4-Hydroxytamoxifen (4-OHT) and Etoposide were obtained from Sigma Aldrich, BafilomycinA1 was purchased from Cambridge Bioscience and BrDU was purchased from Abcam.
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bioRxiv - Plant Biology 2024Quote: ... through quantification of the fluorescence of 4-MU (4-Methylumbelliferone, Sigma) reaction product ...
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bioRxiv - Molecular Biology 2024Quote: ... for 2 h or 4-hydroxytamoxifen (4-OHT, Sigma, 10 µM) for 4 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Mia PaCa-2 cells and PCAFs were then transduced with the respective viral suspension at different multiplicity of infection (MOI) in the presence of 6 µg mL-1 hexadimethrine bromide (polybrene, Sigma H9268). Cells were sorted for mCherry and EGFP fluorescence using a BD FACSAria™ Cell Sorter (BD Biosciences ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... the neurons of postnatal day 1–2 NMRI strain mice were grown 6 DIV on polyornithine-laminin (P3655 and CC095, Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141 ...
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bioRxiv - Molecular Biology 2021Quote: ... BAT was then incubated for 1 hour at 37 °C before stimulation with 10 μM isoproterenol (ISO, Sigma, 54750-10-6). Samples were taken at the indicated time points ...
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bioRxiv - Plant Biology 2019Quote: ... protoplasts were isolated from 6-days-old protonemal cultures by incubation for 30 min in 1 wt% driselase (Sigma-Aldrich, D8037) dissolved in a 8.5 wt% mannitol solution in water ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated 12 min with 5% Triton in PBS and then primary cilia and SMO were stained using a mouse anti-acetylated tubulin Ab (6-11B-1, Sigma-Aldrich) and a rabbit anti-SMO Ab (1:100 ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fabricated with a final concentration of 1×105 AFC/mL (P3-5) and incubated in EGM-2 +/- 1 mg/mL of the plasmin inhibitor 6-aminocaproic acid (Sigma, A2504) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Electroporated PBMCs were transferred into 1 well of a 6 well plate seeded with MEFs in PBMC recovery medium (RPMI supplemented with 200uM 1-thioglycerol (Sigma M6145), 1uM Dexamethasone (Sigma D1756) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were counted and plated at a density of 1 x 106 cells per well of a 6 well plate that was pre-coated with sterile 1% (w/v) gelatin (Sigma-Aldrich). For immunocytochemistry ...
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bioRxiv - Microbiology 2020Quote: Seven-week old Arabidopsis leaves with or without herbivore infestation for 6 h were stained with 1 mg/ml DAB-HCl solution (Sigma, D5637) overnight in darkness ...
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bioRxiv - Cell Biology 2021Quote: HEMs or MNT1 cells seeded in a 6-well plate at 50-80% confluency were lysed in 1% Triton X-100 (T8787, Sigma-Aldrich) and centrifuged at 14,000 RPM for 30 min at 4°C to separate solubilized protein from melanin ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 0.1 M sodium cacodylate buffer containing 1% OsO4 and 1.5% K4Fe(CN)6 (Sigma-Aldrich, St. Louis, MO), en bloc stained with 2% aqueous uranyl acetate ...
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bioRxiv - Evolutionary Biology 2021Quote: Total bacterial counts were determined by plating out 25 µl of a dilution series ranging from 10−1 to 10−6 on LB-agar plates (Sigma-Aldrich). To identify recipient strains (carrying the dTomato marker ...
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bioRxiv - Biochemistry 2021Quote: ... cells were fixed using a 6% (v/v) formaldehyde:phosphate-buffered saline solution and stained with 1% (w/v) crystal violet (Sigma-Aldrich, Italy) in 70% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: Biotin labelled and unlabeled RNA oligonucleotides probes corresponding to the human cMYC ARE (1-6) and two random probes (R1, R2) were synthesized as indicated in Table S5 by Sigma-Aldrich. REMSA was performed with a LightShift chemiluminescent RNA EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and 20 uL of 120 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (Pierce; dissolved in 47% ACN, 47% water and 6% HPLC-grade pyridine (Sigma Aldrich)) were added ...
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bioRxiv - Physiology 2019Quote: Approximately 15 mg of the left TA muscle was homogenized using a glass tissue-homogenizer in 1.5 ml ice-cold lysis buffer (6 M Urea, 2 M Thiourea, 10 mM DTT, Complete Protease Inhibitor (1 tablet per 10 ml lysis, Sigma-Aldrich), PhosSTOP (2 tablet per 10 ml lysis ...
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bioRxiv - Molecular Biology 2020Quote: ... To stabilize beta-catenin and to induce canonical Wnt signaling the cells were treated with 1 μM BIO (6-Bromoindirubin-3′-oxime, SIGMA B1686) for 24 hours before harvesting.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were passaged to single cell suspension and plated to achieve 65,000 cells per well of a 6-well plate in Nutristem supplemented with 1:2,000 Y-27632 (ROCK inhibitor; Sigma Aldrich, Y0503) [23] ...
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bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in StemFlex containing 1 μM H1152 (Millipore, #555550). On the next day ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and the pellet resuspended in 1 ml of BHI containing 10 µM 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFDA-SE, Sigma #21888). The suspension was incubated on a rotating shaker at 37°C and 200 rpm for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Physiology 2020Quote: ... On day 4 cells were re-stimulated with the same concentrations of anti-CD3/CD28 for 6 hours at 37°C in the presence of 1 μg/ml brefeldin-A (Sigma-Aldrich). As a control ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Cas9 viral supernatant was added to 1×106 cells growing in a 6-well plate plate in 3mL of complete media with polybrene (4mg/mL) (Sigma, H9268). The cell-virus mixture was centrifuged at 3000rpm at 25°C for 1h followed by incubation at 37°C overnight ...
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bioRxiv - Genetics 2021Quote: ... The reaction was performed in the presence and absence of an NADPH generating system: 1 mM glucose-6-phosphate (Sigma Aldrich), 0.1 mM NADP+ (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Filtered particles were either immediately introduced to cells (105 target cells plated in a 6-well plate) in antibiotic-free culture medium supplemented with 8 μg ml-1 polybrene (TR-1003-G, EMD Millipore) or stored in single use aliquots at −80ºC ...
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bioRxiv - Physiology 2023Quote: THP-1 monocytes were differentiated into THP1-derived macrophages by stimulating 5×105 THP-1 monocytes/well in 6-well plate with 100 ng/mL phorbol 12-myristate 13-acetate (PMA) (#P8139, Sigma Aldrich) for 48 h ...