Labshake search
Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for 6 7 Dichloro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Cells were fixed using 70% Ethanol and re-suspended in PBS containing 1 μg/ml 4’,6-diamino-2-phenylindole (DAPI, Sigma) and analyzed by microscopy ...
-
bioRxiv - Neuroscience 2022Quote: ... and the supernatant was gently layered on a discontinuous Percoll gradient (2 %, 6 %, 10 %, and 20 % v/v in Tris-buffered 0.32 M sucrose; Sigma-Aldrich). After centrifugation at 33’500 g for 5 min ...
-
bioRxiv - Cancer Biology 2022Quote: Lentivirus was titrated into 2 × 105 PC-3M cells on 6-well plates with 2 mL complete RPMI-1640 supplemented with 8 μg/mL polybrene (Sigma). After overnight infection ...
-
bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
-
bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
-
bioRxiv - Developmental Biology 2022Quote: ... in PBST and then counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 0.001 mg/mL in 1x PBS, Sigma-Aldrich) for 5 min in the dark ...
-
bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (5 μM) and propidium iodide (PI) (50 μg/mL) (Sigma-Aldrich, St. Louis, USA) and incubated at room temperature for 30-60 minutes ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were washed three times in PBS and mounted in 70% glycerol with with 5 μgml-1 concentration of 4-6-diamidino-2-phenylindole (DAPI, Sigma) and cells were mounted on cover slips ...
-
Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... pH 7.4) containing 10 mM sodium azide and 6 mM 2-deoxy-D-glucose (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 50 min ...
-
bioRxiv - Neuroscience 2020Quote: ... 6-month-old littermate matched were injected subcutaneously with vehicle [40% (w/v) (2-hydroxypropyl)-β-cyclodextrin (Sigma– Aldrich #332607)] or MLi-2 dissolved in vehicle at a 30 mg/kg final dose ...
-
bioRxiv - Cell Biology 2021Quote: ... G6P concentrations were determined at 2 hrs and 6 hrs post activation and compared to unactivated controls using a G6P kit (Sigma) and following manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2021Quote: HDMB03 cells (3×105 in 6-well plates) were treated with global de-methylating agent 5-AzaC (5 μM) (5-Aza-2-deoxycytidine; Sigma). Following 96 hours of incubation ...
-
bioRxiv - Cell Biology 2021Quote: ... after which we incubated a 20µL pre-isolation aliquot of the filtrate with 0.1µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Millipore-Sigma, cat. #D9542) and mounted on a slide for imaging (Fisher ...
-
bioRxiv - Cell Biology 2021Quote: ... after which we incubated a 20µL pre-isolation aliquot of the filtrate with 0.1µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Millipore-Sigma, cat. #D9542) and mounted on a slide for imaging (Fisher ...
-
bioRxiv - Microbiology 2020Quote: ... and bacterial nucleoids stained by topping cells with a drop of Fluoroshield™ containing 1.5 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Cover slips were placed over the samples and kept at room temperature for ∼ 30 minutes before cells were imaged.
-
bioRxiv - Physiology 2020Quote: ... Dead cells and debris were excluded by measurements of forward-versus side-scattered light and DAPI (4′,6-diamino-2-phenylindole) (Sigma) staining ...
-
Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... After incubating in primary antibodies for two hours and DAPI (4′,6-diamidine-2′-phenylindole dihydrochloride, Sigma Aldrich, 10,236,276,001 Roche) for the last ten minutes ...
-
bioRxiv - Immunology 2022Quote: ... For cell sorting cell suspensions were stained for viability with 1:3000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) immediately before sorting.
-
bioRxiv - Developmental Biology 2022Quote: ... sections were washed in PBS and then incubated for 10 minutes in 1:10000 4’,6-diamidino-2-fenilindol (DAPI; Sigma) for nuclei counterstaining ...
-
bioRxiv - Genomics 2022Quote: ... Cross-links were reversed by incubating the eluted samples and inputs at 42°C for 2 hours and 67°C for 6 hours in the presence of Pronase (Sigma) and TE buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... the samples were mounted in polyvinyl alcohol-based mounting medium containing 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; FUJIFILM-Wako) and 2.5% (w/v) 1,4-diazabicyclo[2.2.2]octane (Sigma, St. Louis, MO, USA) as an antifade ...
-
bioRxiv - Neuroscience 2020Quote: ... After rinsing sections were incubated 10 min at room temperature (RT) with 0.001 % DAPI (4’,6-diamidino-2-phenylindole dihydrochloride, Sigma, D9542) in PBS for nuclear staining ...
-
bioRxiv - Immunology 2022Quote: ... The sections were washed in PBS and incubated 10 min with 4’,6-diamidino-2-phenylindole (DAPI, 1/10,000, Sigma-Aldrich) to visualize the cell nuclei ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were washed three times with 1x PBS and incubated for 5 minutes in 300 nM 4′,6-diamidino-2-phenylindole (Sigma) before a final 1x PBS wash ...
-
bioRxiv - Developmental Biology 2019Quote: ... Sections were further incubated for 10 min at room temperature with DAPI (4’,6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibodies lasted for 3h at room temperature ...
-
bioRxiv - Cancer Biology 2019Quote: ... and resuspended in 50 to 80 µl FC buffer containing 0.1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Primary tumor samples were stained with CD45-APC-Cy7 (Biolegend ...
-
bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
-
bioRxiv - Immunology 2019Quote: PBMCs (freshly isolated and previously frozen) and fresh whole blood were each stimulated for either 2 or 6 h with (1) PMA/ionomycin (Sigma) (PBMC ...
-
bioRxiv - Cancer Biology 2019Quote: ... Tissues were washed 5 times in PBT and incubated for 2h at RT with secondary antibodies and 4’,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, Sigma) (1µg/mL) ...
-
bioRxiv - Developmental Biology 2020Quote: ... An incubation was done 45 min at room temperature with a mix of 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, 1µg/mL, 10236276001, Sigma-Aldrich) and the secondary antibody Donkey anti-Mouse Alexa Fluor 555 in PBS 1X ...
-
bioRxiv - Cell Biology 2019Quote: ... then stained in 1x PBS + .5M NaCl with 50 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich #D9542).
-
bioRxiv - Immunology 2020Quote: ... Cells were washed once with PBS prior incubation for 5 min at 37 °C with 4′,6-diamidino-2-phenylindole (DAPI; Sigma). Finally ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
-
bioRxiv - Immunology 2022Quote: ... The sections were then washed with PBS/0.5% Triton and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Digitally scanned sections were analysed using Image Scope software (Scanscope XT ...
-
bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Chromosomes were stained with 4′,6-diamidino-2-phenylindole at a concentration of 5 μg/ml (DAPI; Sigma-Aldrich, D8417).
-
bioRxiv - Synthetic Biology 2023Quote: ... All samples diluted with buffer to the final concentration of 2 μM Aβ were supplemented with 6 μM ThT (Sigma), with a range of concentrations of the binders per experiment ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were mounted in Sigma-Aldrich DuoLink in situ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich), allowed to incubate at room temperature (RT ...
-
bioRxiv - Microbiology 2023Quote: ... quenched with 25 mM ammonium chloride in PBS for 10 min and nuclei were stained with 4’,6-diamidino-2-phenylindol (DAPI, Sigma, cat ...
-
bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 30min at room temperature (10µg/mL, D9542, Sigma-Aldrich). Coverslips were mounted with ProLong Gold Antifade (P36934 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... slides were washed for 5 min in 0.1x SSC with 1% Triton X-100 at 62°C and counterstained with 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) in antifade with DABCO (1,4-diazabicyclo (2.2.2)-octane ...
-
bioRxiv - Immunology 2023Quote: 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) was used to exclude dead cells and debris (Sigma Aldrich, St. Louis, MO). All antibodies are from Biolegend ...
-
bioRxiv - Bioengineering 2023Quote: ... samples were washed with PBS and the cells’ nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1.5 μg/mL, Sigma-Aldrich) for 5 min at room temperature in the dark ...
-
p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... mice were fasted for 6 h and received an oral (2 g/kg body weight) glucose load (G7021, Sigma Aldrich). For the insulin tolerance test ...
-
bioRxiv - Biophysics 2023Quote: ... 1.5 – 2 million cells were plated on a 6-well-plate coated with 10 µg/ml fibronectin (Sigma; F1141-5MG) and maintained in IMDM (GlutaMAX ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
-
bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
-
bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
-
bioRxiv - Microbiology 2024Quote: ... The pesticides were: 3,6-dichloropyridine-2-carboxylic acid (i e. clopyralid, pesticide analytical standard, CAS 1702-17-6, supplier: Sigma-Aldrich, purity ≥ 98.0% ...