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Citations for Millipore Sigma :
2101 - 2150 of 10000+ citations for 6 7 Dichloro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: All-trans-astaxanthin and β-carotene (CAS Numbers: 472-61-7 and 7235-40-7) were purchased from Sigma-Aldrich (USA). Lutein was HPLC-purified from a commercial lutein preparation purchased from RealCaps ...
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bioRxiv - Biochemistry 2021Quote: All-trans-astaxanthin and β-carotene (CAS Numbers: 472-61-7 and 7235-40-7) were purchased from Sigma-Aldrich (USA). Liposomes were prepared according to the Racker method [72] with modifications ...
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bioRxiv - Cell Biology 2022Quote: Cannulated lungs from 17-18-month-old male C57BL/6 mice were filled with warm low melting point agarose (3%, Sigma-Aldrich, St. Louis, MO). Lung segments were cooled on ice for 30 min to allow solidification of the agarose ...
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bioRxiv - Immunology 2023Quote: ... 300,000 WT T84 cells per well in a 6-well plate were treated with 20 µL lentivirus using 3 µL Polybrene transfection reagent (Sigma Aldrich #TR-1003-G) diluted in 3 mL media ...
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bioRxiv - Immunology 2021Quote: ... for 1h followed or not by NLRP3 activation with 20 μM nigericin (Sigma-Aldrich, Cat. no: N7143-5MG) for 1.5 h ...
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bioRxiv - Cell Biology 2021Quote: ... differentiated HNEC cultures were incubated for 1h in ALI-differentiation medium supplemented with 5 µg/ml polybrene (Millipore) before infection.
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bioRxiv - Biophysics 2020Quote: ... Micropatterns were printed for 5 min with specifically designed chrome masks and coated for 1h at 5% CO2 and 37°C with 50μg/mL fibronectin and 20μg/mL Alexa 546–fibrinogen (Sigma) diluted in distilled water ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were washed with phosphate sucrose buffer 0,1M, post-fixed (1h, 4°C) with 1 % osmium tetroxide (TAAB Laboratories) in potassium ferricyanide 0,8% (Sigma) and incubated with 2% aqueous uranyl acetate (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... then re-blocked and probed B-tubulin for 1h with the mouse anti-ß-tubulin (1:1000; Sigma). Bands were analysed by area under curve relative to tubulin loading controls (Image Lab™ software).
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bioRxiv - Biophysics 2021Quote: ... and kept at 4°C for 1h in KB solution containing 50 mg/ml polyvinylpyrrolidone (Sigma, Chemical Co.). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... for 1h at RT, blocked for 1h at RT in blocking solution (0.3% Triton X-100, 0.2% BSA (A4503, Sigma), and 5% goat serum (005-000-121 ...
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bioRxiv - Cell Biology 2020Quote: ... 3-Isobutyl-1-methylxanthine (IBMX, ≥ 99%), N-acetyl-L-cysteine (NAC, ≥ 99%) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA, ≥ 97%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pregnant mare serum gonadotropin (PMSG ...
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bioRxiv - Plant Biology 2021Quote: ... leaf epidermal strips were peeled from tobacco leaves and floated on a solution of 50 μM 2’-7’dichlorofluorescein diacetate (DCFH-DA; Sigma Chemicals, St Louis, MO, USA). The leaf stripe samples were collected after salt stress treatment for the indicated time ...
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bioRxiv - Neuroscience 2019Quote: ... transferred into 2-ml Eppendorf cups containing 500 μl of 1 M L-ascorbic acid (Sigma Aldrich, cat. no.: A7506, CAS no.: 50-81-7) and bead-based homogenized for 2 min using a Qiagen TissueLyser LT at a frequency of 50/s ...
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bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were resuspended in 24.5 ml/tube of complete Percoll media (composed of 25 ml of L-15 medium [Life Technologies] supplemented with 0.429 g/L HEPES [Sigma], 2 g/L BSA [Sigma], 1 × 10−7 M insulin [Sigma], 2.4 ml of 10× HBSS(-) [Life Technologies] ...
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bioRxiv - Biophysics 2023Quote: ... and ultrapure H2O and finally incubated in a solution of poly-T ssDNA (100 nM) in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (10 mM HEPES, NaCl 50 mM, pH 7, Sigma-Aldrich, St Louis, MO, USA) for 1 h at approx ...
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bioRxiv - Neuroscience 2022Quote: Sympathetic nervous ablation was performed with 6-hydroxydopamine hydrobromide (6-OHDA, Sigma, H116). Universal ablation of sympathetic nerves was performed in mice by i.p ...
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bioRxiv - Plant Biology 2023Quote: ... plants were sprayed with 100 μM 6-benzyl purine (6-BAP, Sigma-Aldrich) or Mock solution 24h prior to analysis ...
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bioRxiv - Genetics 2021Quote: ... 3 mM MgCl2 (Sigma, 7786-30-3), 0.1% Tween-20 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and EIF4E2 shRNA#3 (sh4EHP#3) (Sigma,). GIGYF2 shRNA#1 (shGIGYF2#1 ...
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bioRxiv - Cancer Biology 2022Quote: ... covered with 3-aminopropyltrimethoxysilane (3-APTS, Sigma) for 3 min for activation ...
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bioRxiv - Neuroscience 2021Quote: ... oxygenated DAB (3-3’-diaminobenzidine, Sigma-Aldrich) was dissolved in 0.1 N HCl at a concentration of 5.4 mg/ml and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... 3-mercaptobenzoic acid (3-MBA, Sigma-Aldrich)-capped gold nanoclusters (3-MBA-Au nanoclusters ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, cat. D8517, Sigma Aldrich, St. Louis, MO, USA) diluted 1:3000 in PBS 1X ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... the neurons of postnatal day 1–2 NMRI strain mice were grown 6 DIV on polyornithine-laminin (P3655 and CC095, Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141 ...
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bioRxiv - Microbiology 2020Quote: ... Tissues submerged in RNAlater solution were mixed with 2 ml of 6% (w/v) betaine (Sigma Aldrich, St. Louis, MO, USA) and placed on ice prior to crushing with mortar and pestle until gut homogenates were formed ...
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bioRxiv - Cancer Biology 2022Quote: ... A21111) Slides were counterstained using 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL in PBS, Sigma-Aldrich, cat. D9542-5MG). Coverslips were mounted using mounting medium (Fluoromount-G™ Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 1x PBS and fixed on slide with methanol followed by DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich) to stain nuclear and kinetoplast DNA and analysed immediately ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed in methanol and then stained with DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich). Cells were visualized using a Olympus IX 81 microscope with FV1000 confocal system using a 100X oil immersion Plan Apo (N.A ...
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bioRxiv - Immunology 2021Quote: ... the slips were washed with PBS three times and stained with DAPI (4’, 6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated in 500 ng/ml 4’,6-diamidino-2-phenylindole (DAPI, diluted in 0.1 M PB, Sigma, 10 min) prior to mounting using Prolong Gold (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... the gels were washed 3 x 20 min with 3.5 ml of ultrapure water and selected specimens were also incubated for 30 min at room temperature with 10 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Sigma, D9542) or 10 µg/ml Hoechst 33342 (Thermo Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fabricated with a final concentration of 1×105 AFC/mL (P3-5) and incubated in EGM-2 +/- 1 mg/mL of the plasmin inhibitor 6-aminocaproic acid (Sigma, A2504) at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... glycans were co-crystallized with a solution of 50% acetonitrile/50% 20 mM ammonium citrate containing 134 mM of 2’,4’,6’-Trihydroxyacetophenone monohydrate (Sigma-Aldrich). MALDI-ToF MS analysis of the N-glycans was performed in the reflector positive and negative ion mode using a 4800 Plus MALDI ToF/ToF Analyzer (AB Sciex ...
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bioRxiv - Biochemistry 2021Quote: ... 1 × 107 PFU per ml of MNV-1.CW3 were incubated at temperatures ranging between 0 and 56°C for 2 or 6 h in the presence or absence of 500 μM bile acid (sodium glycochenodeoxycholate GCDCA, Sigma Aldrich) by enumeration by plaque assay.
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bioRxiv - Systems Biology 2020Quote: ... beads were recovered from the emulsion by washing with 2 × 30 mL 6 × saline sodium citrate buffer (diluted from Sigma #S6639) buffer in a 5 µm ÜberStrainer (pluriSelect) ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with DAPI (4’,6’-diamidino-2-phénylindole) and slides mounted with Fluoromount Aqueous Mounting Medium (F4680, Sigma Aldrich, France) before analysis using Zeiss LSM microscope confocal.
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were diluted in PBS and sections were incubated for 1 hour at room temperature with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for nuclei visualization ...
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bioRxiv - Bioengineering 2022Quote: ... the tissues were incubated overnight at RT and in dark conditions with a 1/400 dilution of Myosin 4 Monoclonal Antibody (MF20; eBioscience) as primary antibody and 4′,6-diamindino-2-phenylindole (DAPI; Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Behaviour was monitored at 500 fps in 6 dpf larvae with their head restrained in 2% low-melting point agarose (Sigma-Aldrich) and their tail free to move ...
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bioRxiv - Genomics 2020Quote: ... The lentivirus containing the pLenti-FNLS-P2A-Puro or pLenti-ABERA-P2A-Puro or desired gRNAs were added to the 0.5 million of HUDEP-2 or HUDEP-2 stable cell line in a one well of the six-well plate along with 6 μg/ml Polybrene (Sigma-Aldrich) and 1% HEPES 1M buffer (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) in PBS to stain nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... age-matched male Tg2576 mice were assigned to receive 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox, Sigma-Aldrich, St. Louis, MO) by intraperitoneal injection for 1 week ...
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bioRxiv - Immunology 2020Quote: ... Then the slips were washed with PBS for three times, and after that the hemocytes were stained with DAPI (4’, 6-diamidino-2-phenylindole) (50 ng/ml) (Sigma, USA) for 5 min [55] ...
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bioRxiv - Microbiology 2021Quote: ... cells from control and experimental culture were washed with PBS and mounted with DAPI (4’,6-diamidino-2-phenylindole) over glass slides using slide mounting media from Sigma-Aldrich and observed under the fluorescent microscope ...
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bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature in the dark and incubated with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich) for 10 min ...
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bioRxiv - Physiology 2019Quote: Approximately 15 mg of the left TA muscle was homogenized using a glass tissue-homogenizer in 1.5 ml ice-cold lysis buffer (6 M Urea, 2 M Thiourea, 10 mM DTT, Complete Protease Inhibitor (1 tablet per 10 ml lysis, Sigma-Aldrich), PhosSTOP (2 tablet per 10 ml lysis ...