Labshake search
Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: Exponentially growing cells at OD600 nm = 0.2 were injected into the microfluidics chambers of a CellASIC plate (Y04C-02 plate for haploid cells, CellASIC ONIX2 Microfluidic System, Millipore), through the inlet wells ...
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bioRxiv - Neuroscience 2019Quote: ... clones were split with pre-warmed 62 µl (96-well plate) or 125 µl (48-well plate) of Accutase (Millipore) in a 1:2 ratio ...
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bioRxiv - Plant Biology 2022Quote: Col-0 and rve 4 6 8 seedlings were germinated on 0.5x MS control plates and 0.5x MS plates supplemented with 50 μM MG132 (Millipore Sigma; 474787) and grown under 12:12 photoperiod conditions as described above for 6 days prior to imaging.
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bioRxiv - Microbiology 2021Quote: Cells obtained from overnight cultures grown in YPD broth at 37°C were spotted in serial 10-fold dilutions on YP plates containing 2% glycerol or YPD plates containing 0.05 mg/ml of Congo red (Millipore Sigma) or 0.01 mg/ml of calcofluor white (Millipore Sigma) ...
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bioRxiv - Immunology 2021Quote: Panning was performed against monomeric NTD directly absorbed to polystyrene plates (Maxisorb 96-well microtiter plates; Nunc, Sigma-Aldrich, USA). Blocking of plates and phages was conducted for 60 min using two blocking solutions ...
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bioRxiv - Synthetic Biology 2023Quote: HEK293T-LP cells sourced from Matreyek et al38 were plated into a 48 well plate according to the plate reader assay and treated with 0 to 2 ng/μl dox (Sigma) to induce BFP expression ...
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bioRxiv - Microbiology 2023Quote: ... subtilis were streaked onto Tryptic Soy Agar (TSA) plates (NYU Reagent Preparation Core) and Enterococcus species were streaked onto bile esculin azide (BEA) plates (Millipore) and incubated overnight at 37°C ...
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bioRxiv - Physiology 2023Quote: ... were transferred to OP50-seeded plates or RNAi-seeded NGM plates supplemented with 50 μM of 5-fluoro-2-deoxyuridine (FUDR, Sigma) to prevent progeny growth ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then recovered in 10ml of YES at 25°C overnight and subsequently inoculated on selective plates - YES plates containing 100µg/mL G418 sulphate (Sigma Aldrich), 50µg/mL hygromycin B (Sigma Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... we used GFP Salmonella and GFP Listeria and plated the samples on Kanamycin plates for the Salmonella and Listeria selective enrichment supplement containing plates (Sigma) for the Listeria ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were lysed and processed for cell death detection ELISA assay by following the manufacturer’s instruction (#11774425001, Millipore Sigma). At the end of the assay ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plates were then rewashed three times with PBST before the addition of 3,3’,5,5’-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, UK) and incubated for 12 minutes in the dark at RT ...
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bioRxiv - Immunology 2019Quote: ... The peritoneal lavage fluids were collected and concentrated for ELISA analysis with Amicon Ultra 10 K filter (UFC900308) from Millipore. Peritoneal exudate cells were collected and analyzed by FACS.
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bioRxiv - Microbiology 2021Quote: ... and stored at −80°C until particle concentration assessment by p24 ELISA (Innogenetics) while the rest of the supernatant was concentrated by filtration on Centricon units (Centricon Plus-70/100K, Millipore) and frozen at −80°C for RNA extraction ...
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Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsybioRxiv - Neuroscience 2023Quote: ... Lysates were removed and protein antibody-conjugates linked to the bottom of the plate were washed three times with 300 µL of ELISA wash buffer (100mM Tris buffered saline, pH8.2, 2mM MgCl2, 0.5% Tween-20 – all Sigma-Aldrich, Ireland). Protein was eluted by incubating wells in 2 X Laemelli buffer for 10 mins ...
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bioRxiv - Immunology 2022Quote: ... antibody levels were detected using a secondary anti-murine IgG conjugated with alkaline phosphatase and the substrate for alkaline phosphatase yellow (pNPP) liquid substrate system for ELISA (cat. P7998, Sigma). After 30 minutes of incubation ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each and 100 μl of blocking solution containing goat anti-mouse IgG (Sigma 1:3,000) was added to each well and incubated at 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... dried under a stream of nitrogen and reconstituted in 25 μL of chloroform:methanol (2:1, vol/vol) and loaded on a TLC plate (Sigma Aldrich Cat #1118450001). The plate was activated using 1.8% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Cells on the surface of the plate were then lysed in the Triton lysis buffer (1% TritonX-100 [Sigma-Aldrich T8787], 137 mM NaCl [Sigma-Aldrich S9888] ...
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bioRxiv - Microbiology 2021Quote: ... the pegged lid was removed and washed with gentle agitation for 1 min in two new Nunc plates each containing 180 μl of phosphate buffer solution (PBS; Millipore Sigma, Canada). The pegged lid was then transferred to a new plate containing 180 μl of 0.1% crystal violet (Millipore Sigma ...
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bioRxiv - Microbiology 2021Quote: ... aspergillus minimal media (AMM) [33] were added in pre-wetted 96-well MultiScreen Filter Plates HTS (1 μm glass fiber filter, Merck Millipore, Darmstadt, Germany) and incubated for 15 min at 37°C with either 25 μL PBS or 25 μL [Fe]TAFC solution (control – uptake block ...
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bioRxiv - Immunology 2021Quote: ... Total cell extracts were prepared and bound to Nunc MaxiSorp plates (eBioscience, Frankfurt, Germany) precoated with murine anti-FLAG mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... Sterilized Arabidopsis seeds were spread on agar plates containing 1⁄2 strength Murashige and Skoog (MS) medium with 1% sucrose and 0.8% agar (A1296; Sigma, St. Louis, MO, USA) and stratified at 4 °C in the dark for 2 days ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We diluted the saturated culture 1:10 in 96-well plates containing 200 μL of SC[MSG] -ura -leu containing Estradiol (β-estradiol, Sigma-Aldrich) at different concentrations (0 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Coating solution was then removed by flicking and plates were washed with OptiMEM and blocked with 25 ul/well of assay buffer (1% Ovalbumin (Sigma #A5503-10G) in OptiMEM containing 1X Pen-Strep (Gibco #15140-148)) ...
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bioRxiv - Immunology 2021Quote: ... BMDC clusters were harvested on day 8-10 in 5 mls of comlete I-10 media at room temperature and then stimulated at 1-2x106 cells per well of an untreated 6-well plate (Sigma M9062-100EA) in 2 mls of I-10 medium without GMCSF.
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bioRxiv - Cell Biology 2019Quote: ... 15,000 THP1 cells were seeded per well in 384 well plate and were transfected with either universal negative control 1 (UNC1) (Millipore Sigma, SIC001) or esiRNA human TFEB (Millipore Sigma ...
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bioRxiv - Immunology 2022Quote: ... and the plate was blocked with 200 µL blocking solution (1% BSA [Sigma, #A9418-5G], 0.05% Tween [Sigma, #P9416-100ML], in PBS). The solution was removed and sample or standard curve (made with mouse IFN-β [PBL Biomedical Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... and seeded at 1 x 105 in a 24 well plate and then pulsed with 1ng/mL OVA peptide (Sigma, #S7951-01mg) for 3 hours ...
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bioRxiv - Biophysics 2022Quote: ... After lipid extraction against organic solvent — CHCl3/MeOH 2:1 (vol/vol) — samples were spotted on silica plates (Sigma-Aldrich, Steinheim, Germany) with the automatic TLC sampler 4 (CAMAG ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Cell Biology 2023Quote: Zoospores were adhered for 5 min to plasma cleaned 96-well plates (MatriPlate, Cat. No. MGB096-1-2-LG-L) coated with 0.5 mg/mL Concanavalin A (Sigma, Cat. No. C2010). We fixed the cells in 4% PFA and 50 mM sodium cacodylate ...
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bioRxiv - Synthetic Biology 2023Quote: Colonies were screened using stereoscope images of LB agar inducer plates with 1 mM potassium tetrathionate (Sigma Aldrich, catalog no. P2926-25G), 1 mM isopropyl-beta-D-thiogalactoside (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... the plates were assayed on a GloMax® Explorer Multimode Microplate Reader after incubation with Resazurin (16 µg ml-1; Sigma-Aldrich) for 4- 6 h.
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bioRxiv - Plant Biology 2023Quote: For the protoplast transfection study Arabidopsis seeds were surface sterilized and sown on in vitro plates containing 1 % agar (Sigma-Aldrich France) as previously described (David et al. ...
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bioRxiv - Immunology 2024Quote: ... and seeded between 0.5×105 – 1×105 per well in 96 well plate coated with 10 µg/mL Poly-D-lysine hydrobromide (Sigma – Cat. P7886). The medium culture was changed three hours after the seeding and every two days until the seventh day when the cells developed a typical morphology of a mixed neuron culture.
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bioRxiv - Cell Biology 2023Quote: ... Gels were transferred back to a 24-well plate and incubated with 0.5 ml PBS BSA 3% with secondary antibodies AlexaFluor™ 488 goat anti-rabbit (A11008, Sigma, 1:500), Cy3-streptavidin and 0.1 μg/ml DAPI (D9542 ...
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bioRxiv - Neuroscience 2024Quote: ... were trained on a 2.5% agar plate (100mm petri dish) covered with 2 mL of 1 M sucrose solution (SUC, Sigma, Cat. No. S1888) or 0.1% quinine hemisulfate solution (QUI ...
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bioRxiv - Microbiology 2024Quote: ... Overnight cultures of the desired bacterial strains were prepared in microtiter plates using 200 µL of TSB supplemented with 1 % (w/v) glucose (Sigma-Aldrich, UK) to facilitate biofilm formation (Croes et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... 1×105 isolated neutrophils were added to a 96-well black walled plate and stained with 100mM luminol (Sigma-Aldrich, MO, USA) followed by stimulation with 500nM phorbol 12-myristate 13-acetate ...
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bioRxiv - Cell Biology 2024Quote: ... Worms were synchronized by bleaching and Day 1 adults were plated on NGM plates containing 100 µM 5-Fluoro-2′-deoxyuridine (FuDR) (Sigma Cat. F0503) with or without 4 mM methyl viologen dichloride hydrate (paraquat ...
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bioRxiv - Immunology 2024Quote: ... The minced tumor tissue was transferred to a 6-well plate containing 3 mL/well of tumor digestion media (NK cell media + 1 mg/mL collagenase IV (Sigma, cat. C5138) + 0.2 mg/mL DNAse I (Roche ...
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bioRxiv - Immunology 2024Quote: Phagocytosis capacity was assessed for 500’000 PBMCs per well in a 24-well plate in 500µl medium (RPMI with 10% FBS, 1% penicillin-streptomycin) supplemented with 20% of human AB serum (Sigma-Aldrich Cat.# H3667). PHrodo E ...
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bioRxiv - Microbiology 2024Quote: ... and 2.5 µl of each dilution was inoculated onto THY or THY-Tween plates containing or not 20 µg.ml-1 polymyxin B (Sigma-Aldrich, Ref. 81271). Plates were incubated at 37 °C for approximately 24 h and photographed ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 monolayers are washed and incubated for 5-6 days at 37 degrees after which plates are scored using the vitality marker WST-8 (colorimetric cell counting kit, Sigma Aldrich, cat 96992-3000TESTS-F). To this end ...
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bioRxiv - Cell Biology 2021Quote: VCAM-1 in the media (1:100) from MCECs stimulated with MG was measured using commercial kits from Sigma, according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The plate was blocked with 5% BSA (Sigma) in PBS for 3 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... aeruginosa-specific cetrimide agar plates (Sigma Aldrich, 22470).
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bioRxiv - Developmental Biology 2021Quote: ... TLC plates were sprayed with Primuline (SIGMA 206865) (1 mg/ml fresh stock in ultrapure water ...