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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... wild and transgenic seeds were grown on 0.8% agar-solidified 1/2 MS vertically oriented plates for 7 days with or without specific concentration of Propyzamide (Sigma-Aldrich). To detect the morphology of the cells of 7-day-old seedlings ...
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bioRxiv - Biophysics 2019Quote: ... Of each lipid 10µl at 3mM dissolved in chloroform/methanol (3:1) were deposited in a glass plate coated with indium tin oxide with about 100Ω resistivity (Sigma-Aldrich) and placed 60 minutes in the desiccator for complete solvent evaporation ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were plated overnight at 1 × 106 cells/well in six-well plates coated with poly-L-lysine (Sigma-Aldrich) and transfected the following day using PEI reagent according to the published optimized procedures69 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were passaged to single cell suspension and plated to achieve 65,000 cells per well of a 6-well plate in Nutristem supplemented with 1:2,000 Y-27632 (ROCK inhibitor; Sigma Aldrich, Y0503) [23] ...
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bioRxiv - Neuroscience 2021Quote: ... slices were transferred to a 12-well plate with 1 mL freshly oxygenated ACSF and incubated with either 1mM anisomycin (Sigma #A9789) or vehicle (0.5% DMSO ...
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bioRxiv - Microbiology 2021Quote: ... aeruginosa strains were routinely refreshed from frozen stocks at -80°C and maintained on YPD (1% Bacto yeast extract, 2% Bacto peptone, 2% dextrose, 2% Bacto agar) plates or Pseudomonas isolation agar (Sigma-Aldrich) for in vitro experiments and LB agar (10 g/liter Bacto tryptone ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in StemFlex containing 1 μM H1152 (Millipore, #555550). On the next day ...
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bioRxiv - Immunology 2020Quote: ... 1×105 neutrophils were seeded into a white 96-well plate (Costar 3917) in the presence of the luminol reagent (1 mmol/L; Sigma-Aldrich). Conidia (MOI 1:2 ...
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bioRxiv - Plant Biology 2021Quote: ... The sterilized seeds were sown on ½ Murashige and Skoog (MS) plates with 1% sucrose and 0.6% agar (Millipore-Sigma cat# A1296). After stratification at 4°C for 48 hours ...
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bioRxiv - Plant Biology 2021Quote: ... The sterilized seeds were sown on ½ Murashige and Skoog (MS) plates with 1% sucrose and 0.6% agar (Millipore-Sigma cat# A1296). After stratification at 4°C for 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were coated with collagen type I at diluted 1:100 ratio with PBS (solution from rat tail) (Sigma-Aldrich, # C3867) for 1 h at 37°C ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: PSCdMs and PAMs were plated in triplicate on non-coated 96-well tissue culture plates at 1×105/well in 100 μl Macrophage Maturation medium or RPMI-1640 (Sigma, #R5886), 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 5 times with PBS/0.5% Tween-20 and 50µl of HRP-anti-mouse-IgA (Sigma-Aldrich, 1:1000) was added to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were grown overnight to exponential phase and five serial dilutions (1/4) of cells were spotted on agar plates using a replica plater (Sigma R2383). Plates were incubated at the relevant temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Filtered particles were either immediately introduced to cells (105 target cells plated in a 6-well plate) in antibiotic-free culture medium supplemented with 8 μg ml-1 polybrene (TR-1003-G, EMD Millipore) or stored in single use aliquots at −80ºC ...
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bioRxiv - Plant Biology 2022Quote: ... seedlings were grown vertically on half-strength MS plates supplemented with 1% (w/v) sucrose and 0.5% (w/v) Phytagel (Sigma-Aldrich, P8169). Five-day-old seedlings were moved to liquid half-strength MS medium containing 2 μM synthetic RALF1 peptide (Sangon Biotech Co ...
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bioRxiv - Microbiology 2022Quote: ... The culture plates were prepared by coating with 0.001% poly-L-lysine solution (Millipore Sigma, P4707, diluted in water 1:10) for plastic surfaces and 100 µg/ml poly-L-lysine hydrobromide (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... faecalis wild-type OG1RF and OG1RF ΔmptD were sub-cultured to OD600 of 0.01 in a 24-well microtiter plate containing 1 mL of Tryptone Soya Broth without dextrose (TSBd, Sigma-Aldrich, USA) supplemented with or without mannose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Equal numbers of cells were lysed in 96-well plates using 100 μl lysis buffer (1% Triton X-100 (Sigma Aldrich), 25 mM Tricine (VWR) ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were washed as before and incubated with an HRP-conjugated anti-FLAG antibody (clone M2, Sigma-Aldrich, 1:10.000 dilution) for 1 h at RT ...
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bioRxiv - Genomics 2022Quote: ... K562 or Jurkat cell lines expressing CRISPRi effectors of interest were infected with lentivirus containing sgRNA expression vectors by centrifugation at 1000 × g for 1 h in 24-well plates in the presence of 8 µg/mL polybrene (Sigma-Aldrich). RPE1 ...
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bioRxiv - Neuroscience 2022Quote: ... 20 young adult animals were placed in the center of an unseeded NGM agar plate supplemented with 1 mM aldicarb (Sigma-Aldrich). Animals were scored every 10 or 20 min as paralyzed if they did not move when gently touched with a platinum wire on the tail ...
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bioRxiv - Physiology 2023Quote: THP-1 monocytes were differentiated into THP1-derived macrophages by stimulating 5×105 THP-1 monocytes/well in 6-well plate with 100 ng/mL phorbol 12-myristate 13-acetate (PMA) (#P8139, Sigma Aldrich) for 48 h ...
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bioRxiv - Immunology 2022Quote: ... the PBMC and CD4+ cell fractions were then added to either HCMV-infected or uninfected autologous primary dermal fibroblasts at E:T ratios of 10:1 in 24-well plates in Roswell Park Memorial Institute (RPMI)-1640 media (Sigma-Aldrich, UK) supplemented with 10% FBS and 100 units/ml of penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... The HLA complexes bound to the beads were then directly eluted into preconditioned Sep-Pak tC18 100 mg Sorbent 96-well plates (Waters, 186002321) using 1% trifluoroacetic acid (TFA; Sigma Aldrich). After two washes of 1mL 0.1% TFA ...
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bioRxiv - Neuroscience 2023Quote: ... 1×105 cells were plated on a 12-well cell culture multiwell plate containing poly-D lysine-coated coverslips (Sigma-Aldrich). After 24 hours ...
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bioRxiv - Cell Biology 2023Quote: Human neuroblastoma SH-SY5Y cells (1 ×105) were seeded into a 24-well plate coated with poly-D-lysine (p6407, Sigma-Aldrich) one day before pseudo-lentiviral particle transduction ...
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bioRxiv - Microbiology 2023Quote: ... Βecton Dickinson Company) broth or agar plates or TY medium (48) in the presence of 1-5 µg/mL thiamphenicol (Sigma-Aldrich) or anhydrotetracycline (ATc ...
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bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... and GSC #161) were obtained from the Mayo Clinic [44] and cultured on laminin-coated plates (1–2 μg/cm2) (Sigma #L2020) in StemPro neural stem cell (NSC ...
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bioRxiv - Immunology 2023Quote: ... The capture matrix was washed three times with PBS and incubated over night with 1:5 diluted plasma samples in a low protein binding MultiScreen® filter plate (Millipore). Depleted plasma was recovered by centrifugation of the filter plate ...
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bioRxiv - Physiology 2023Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatine type B (Sigma-Aldrich). Explants were maintained in RPMI ...
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bioRxiv - Neuroscience 2023Quote: ... and seeded at approximately 1 million cells/cm2 onto assay plates coated with poly-L-ornithine 44 µg/mL (Sigma P3655) and fibronectin 1 µg/mL (Sigma F1141 ...
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bioRxiv - Microbiology 2023Quote: ... and each transferred to its own well in a 96-well growth plate containing 1 mL of LB broth (Sigma-Aldrich) with 50 µg/mL kanamycin ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
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bioRxiv - Neuroscience 2023Quote: ... Tissue was gently disaggregated and neurons plated for 90 minutes with DMEM 10% HS medium in P6 well plates previously treated with 1 mg/ml poly-L-lysine (Sigma Aldrich) at a density of 300,000 cells/cm2 ...
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bioRxiv - Neuroscience 2023Quote: ... the neurons were plated in 4-well plates coated with poly-L-lysine (1 mg/ml) (Cat. #: P2636-500MG, Sigma-Aldrich) and homemade laminin (Chauvet et al. ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 24 h in 24-well plates in complete culture media supplemented with 50 ng/mL PMA and 1 μg/mL ionomycin (Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biophysics 2023Quote: Mechanically passaged hiPSCS were plated on 10 µg/mL Vitronectin XF in 6 well TC plates in 1 μM all-trans-RA (Cat. No. R2500-25MG, Sigma-Aldrich) to NutriStem® hPSC XF Medium (Growth Factor-Free)(Cat ...
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bioRxiv - Cell Biology 2024Quote: ... were produced by transfecting HEK293T cells plated at a density of 1 × 107 cells/15 cm plate one day prior to transfection using polyethyleneimine (Linear PEI, MW 25 kDa, Sigma-Aldrich) as the transfection reagent ...
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bioRxiv - Genomics 2024Quote: ... 1:1000 and 1:10,000 dilutions of the outgrowth were plated on LB plates carrying 50 μg/mL kanamycin (LB+Kan50, Sigma-Aldrich #K1377) and incubated at 37°C for 16 hours ...
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bioRxiv - Microbiology 2019Quote: ... 135 µL of culture was distributed in a 96-well plate (Cellstar, 96 Well Cell Culture Plate, U-bottom) and 15µL of H2O or Mitomycin C (Sigma)) was added at the appropriate dilution ...
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bioRxiv - Synthetic Biology 2021Quote: ... media in each well was replaced with 100µL (for 96-well plate format) or 200µL (for 48-well plate format) complete DMEM in addition to 2µM ionomycin (Sigma-Aldrich) and 5mM CaCl2 with or without 10µM furimazine (unless indicated otherwise) ...
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bioRxiv - Neuroscience 2022Quote: ... and seeded at a density of 2 × 106 cells per well (6-well plates) or 0.5 × 106 cells per well (24-well plates) into PDL/Laminin (Sigma)-coated plates with neuronal medium plus 0.5 μM RA ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... Dilutions were plated on TY plate and in TY plate containing either 750 µM of DEA NONOate (Sigma-Aldrich), 0.1% of NaClO (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Lipodisq® Styrene:Maleic Anhydride Copolymer 2:1 (Kit (Sigma-Aldrich, MO, USA) was used for the detergent-free membrane fraction purification ...
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bioRxiv - Cancer Biology 2023Quote: ... and BIT (bovine serum albumin 4 g/L, insulin 5 µg/mL, and transferrin 60 µg/mL, all from Sigma-Aldrich, Saint Louis, MO, USA). To facilitate optimal cell growth and maintenance ...