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Citations for Millipore Sigma :
2201 - 2250 of 10000+ citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2019Quote: ... The resulting hybridomas were screened by ELISA for reactivity with STm whole cells and purified LPS (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Microbiology 2021Quote: ... Biotinylated IFX fragments were immobilised on a streptavidin-coated plate and binding of the six mouse monoclonal antibodies was tested by ELISA and detected with an alkaline-phosphatase-conjugated anti-mouse secondary antibody (Sigma-Aldrich) as previously described43 ...
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bioRxiv - Immunology 2022Quote: ... The HIV status of all participants was determined by fourth generation enzyme linked-immunosorbent assay (ELISA) testing and HIV RNA plasma viral load (Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Immunology 2023Quote: ... We confirmed the specificity of our IgA ELISA and non-cross reactivity toward IgG using purified human IgG and IgA (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... The major components of the assay included: 1) rS-RBD coating of plates as target antigen (2ug/ml in Sigma Carbonate-Bicarbonate Buffer); 2 ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunofluorescence using primary polyclonal antibodies that label neurons (NeuN, mouse; 1: 1000, Millipore MAB377, Merck Millipore), reactive astrocytes (GFAP ...
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bioRxiv - Cancer Biology 2020Quote: ... the remaining plates were fixed daily from day 2 to day 5 (excluding day 1) prior to staining with crystal violet (Sigma-Aldrich, C0775-100G) for 3-5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Around 1 x 107 cells were cross-linked directly in the culture plate with culture medium supplemented with 1 % of formaldehyde (Sigma-Aldrich; Cat#: F8775) and 15 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... U373MG cells were seeded at a density of 1×104 (24 and 48hr exposure time points) in 96 well plates (Sigma-Aldrich, Arklow, Ireland) with 100µl media per well ...
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bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl of each stock was then spotted onto the surface of YPD plate (Yeast Extract 1%, Peptone 2%, Dextrose 2%, Agar 2%) without and with 1µg/mL tunicamycin (Sigma-Aldrich Inc., MI, USA). Plates were then incubated for 48-72 hours at 30°C before capturing their photographs ...
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bioRxiv - Cell Biology 2022Quote: ... or 1 ml (6-well plates) of lysis buffer (Tris 50 mM; NaCl 150 mM; pH 7.3; Triton X-100 1 % (Sigma-Aldrich. Cat. Nº: T9284); Leupeptin 5 µM (Focus Biomolecules ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 μg ml-1, Sigma-Aldrich, St. Louis, MO, USA) and cultivated in DMEM medium (PAA Laboratories GmbH ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the RAW264.7 cells were seeded at 1×105 cells/well on a 96-well culture plate (Corning® Costar®, Sigma-Aldrich, Australia) for 48 h ...
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bioRxiv - Cancer Biology 2023Quote: 4×103 SW756 or HeLa cells were cultured in medium according to (51) in 48-well plates coated with 1% low attached agarose (Sigma Aldrich, Taufkirchen, Germany). For heterospheroids 4×103 SW756 and 2×103 Th17 cells were used ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish larvae were anesthetized with 1 mg/ml Tricaine and transferred to a 6-well glass-bottom plate containing 3% methylcellulose (Sigma-Aldrich, MO, USA) in E3 medium ...
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bioRxiv - Neuroscience 2023Quote: ... the isolated ganglia were immediately transferred into a 24-well plate containing digestion solution (1 ml high glucose DMEM and 100 μl Collagenase Type IV (Sigma; C1889-50 mg) and incubated at 37°C for 40 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... whilst AL-PHA assay plates were centrifuged 500 g for 10 minutes (MPS 1000 Mini PCR Plate Spinner, Sigma-Aldrich, MO, USA) in order to pellet AL-PHA beads ...
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bioRxiv - Microbiology 2021Quote: ... serially diluted 1:5 and spotted into agar plates with and without indicated additives using a replica plater (Replica plater for 96-well plates, Sigma Aldrich, #R2383). Images of the plates were then taken using Syngene GBox Chemi XX6 Gel imaging system ...
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bioRxiv - Neuroscience 2023Quote: ... the contents of the wells were removed by placing the plate on a Magnetic 96-well plate Separator (Millipore, Burlington, MA, US) for 1 minute to allow the magnetic particles to remain bound ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of each dilution was spotted on YE plates or YE plates containing 7.5 mM hydroxyurea (Sigma-Aldrich, St. Louis, MO), and cells were grown at the indicated temperatures for 3 d ...
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bioRxiv - Biophysics 2023Quote: ... and plate three 10 µL droplets of each dilution on plates made of 2% Lennox LB Broth (Sigma Aldrich, St. Louis, Missouri) and 1.5% agar (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were then transferred from the 96-well plates into opaque white 384-well plates that were pre-coated with monoclonal anti-FLAG M2 antibody (Millipore Sigma, F1804). Plates were blocked with 1% BSA/5% sucrose/0.5% Tween 20/1xPBS ...
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bioRxiv - Plant Biology 2024Quote: ... Arabidopsis seedlings were grown for three days on 0.5x MS plates at 17 °C and then transferred to 0.5x MS plates containing 50 µM TG003 (Sigma-Aldrich, Catalogue No.: T5575) or an equivalent volume of DMSO as solvent control and grown for additional two days at 17 °C to allow for uptake of the compound ...
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bioRxiv - Neuroscience 2021Quote: ... plates were incubated with 0.5mg/ml MTT (SIGMA #M2128) for 3 hours at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: Luria-Bertani agar plates supplemented with ampicillin (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... Plates were washed and developed with p-NitrophenylPhosphate (Sigma). 405nm absorbance was detected using a SPECTRAmax190 plate reader (Molecular Device).
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bioRxiv - Immunology 2021Quote: ... enzyme-linked immunosorbent spot (ELISPOT) multiscreen filtration plates (Millipore) were activated with absolute ethanol ...
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bioRxiv - Cell Biology 2020Quote: ... The plates were coated with 0.01% gelatin (Sigma-Aldrich) prior to seeding.
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bioRxiv - Immunology 2019Quote: ... 96 well ELISPOT plates (Multi-Screen HA Filtration, Millipore) were coated overnight with anti-IgM (331 ...
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bioRxiv - Neuroscience 2019Quote: ... The plate was sealed with AbsorbMax film (Sigma-Aldrich) and incubated at 37°C ...
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bioRxiv - Genetics 2019Quote: ... in Multiscreen PCR cleaning plates (Millipore, Burlington, Massachusetts, USA) with vacuum application (10 psi) ...
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bioRxiv - Molecular Biology 2019Quote: ... plate 2 is treated with doxycycline (Sigma, D9891-25g) at a final concentration of 2 μg/ml 24 hours after duplication for visualization of EGFP+ colonies (with high levels of EGFP expression serving as a proxy for high dCas9 expression) ...
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bioRxiv - Cell Biology 2019Quote: ... Lipid extracts were loaded onto a TLC plate (Millipore) along with egg and milk sphingomyelin as lipid standards ...
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bioRxiv - Immunology 2019Quote: ... Plates were coated with 0.01% poly-L-Lysine (Sigma) for 5 min at room temperature and then dried for 30 min at 42°C ...
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bioRxiv - Immunology 2019Quote: ... Plates were revealed by phosphatase alkaline substrate (Sigma-Aldrich), and absorbance was measured at 450 nm minus 540 nm ...
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bioRxiv - Microbiology 2019Quote: ... Plates were covered with AeraSeal film (Sigma-Aldrich, #A9224) before incubation for 24 hours at 28 °C ...
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bioRxiv - Cell Biology 2020Quote: ... coated plates in DMEM high-glucose (Sigma-Aldrich, D5671) supplemented with 15% FBS (Gibco ...
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bioRxiv - Immunology 2019Quote: ... was added to plates followed by Streptavidin peroxidase (Sigma). Plates were developed with ABTS (10% 2,2’azino 3-thyl benzthiazoline ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed before adding Sigmafast BCIP/NBT (Sigma) substrate solution ...
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bioRxiv - Microbiology 2021Quote: ... plates pre-coated with poly-L-lysine (Sigma–Aldrich) were seed with 293T cells and transfected the following day with 1,200 ng of empty plasmid and 400 ng of plasmid encoding coronavirus spike or no-spike plasmid control (green fluorescent protein (GFP)) ...
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bioRxiv - Neuroscience 2019Quote: NGM plates were prepared with histamine (HA, Sigma, 10mM) as previously described [54] ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was developed with fast kinetic TMB (Sigma) and quenched with 1M HCl ...
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bioRxiv - Bioengineering 2020Quote: ... a Greiner 384-well destination plate (Sigma-Aldrich M1811) was filled with 80 µL of the supplemented M9 medium ...
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bioRxiv - Neuroscience 2021Quote: ... or cell plate wells were covered with glycerol (Sigma). We used the following primary antibodies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The plate was covered with aeroseal rayon film (Sigma) and grown for 48 hours at 30C without shaking ...
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bioRxiv - Cell Biology 2020Quote: ... The plate was washed with 5% Hellmanex Ⅲ (Sigma) overnight and 5 M NaOH for three times ...
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bioRxiv - Biochemistry 2020Quote: ... Fixed plates were stained with 0.05% sulforhodamine B (Sigma) in 1% acetic acid ...