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Citations for Millipore Sigma :
1951 - 2000 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... we diluted fluorescently tagged BSA protein (Sigma-Aldrich, St. Louis, MO) to a concentration of 0.1 mg/mL and encapsulated the solution in 45-μm droplets using the single-emulsion droplet-generation chip ...
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bioRxiv - Biochemistry 2024Quote: ... A dilution series of the protein synthesis inhibitor cycloheximide (Sigma 01810) was created in water ...
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bioRxiv - Biochemistry 2024Quote: Fusion proteins were expressed in BL21(DE3)pLysS E.coli (EMD Millipore). The proteins were tagged at the N-terminus with his6-HT7 (pET30-HT7 vector) ...
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bioRxiv - Bioengineering 2024Quote: Proteins were extracted from fibroblast cells using RIPA Buffer (Sigma #R0278) with protease inhibitors (Roche #11836153001) ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentrated protein underwent further processing with centrifugal filter units (Millipore). The purified SsCBE2-C2 protein was then stored at -80°C.
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bioRxiv - Bioengineering 2024Quote: ... Protein concentration was calculated following Bradford assay (Bradford reagent, Sigma-Aldrich). A 1:50 mixture of β-mercaptoethanol (Acros Organics ...
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bioRxiv - Synthetic Biology 2021Quote: ... proteins were transferred to PVDF membranes and blocked with 5% BSA (Sigma) followed by probing with mAb MoPn-40 (1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... the proteins were transferred to an Immobilon P membrane (EMD Millipore, IPVH00010) at 25°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were eluted with 40 µl 3x FLAG peptide (Sigma-Aldrich) and collected by centrifugation for 30 min at 100xg.
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bioRxiv - Cancer Biology 2021Quote: ... separated protein bands were transferred into polyvinylidene fluoride membranes (Millipore, Cat. #IPVH00010). Then ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) and the membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat dry milk for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was wet transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Millipore) in Criterion blotter cells (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with 50 kDa cut off ...
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bioRxiv - Cell Biology 2020Quote: ... otherwise all other protein labelling were using 1% Triton X100 (Sigma-Aldrich) in PBS permeabilization for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... followed by the addition of 5 mg protein A beads (Sigma, P1406) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... 20µl of DBX-washed Magna ChIP Protein W+G magnetic beads (Millipore) were added to each sample and rotated for 1hr at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Protein concentration of tissue lysates was determined using bicinchoninic acid (Sigma-Aldrich). Proteins were denatured with gel loading buffer (50 mM Tris [pH 6.8] ...
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bioRxiv - Microbiology 2021Quote: ... Protein complexes were purified with α-FLAG M2 affinity gel (Sigma-Aldrich) as described for co-IP ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were electroblotted to polyvinylidene fluoride membranes (IPFL00010, EMD Millipore, Billerica, MA), incubated with primary antibodies (see Supplemental Table 1 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Proteins were visualized after staining with ProteoSilver Silver Stain Kit (Sigma-Aldrich) (S.Fig.1 ...
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bioRxiv - Immunology 2020Quote: ... and 50 μl of horseradish peroxidase-Staphylococcal protein-A conjugate (Sigma-Aldrich) diluted 1:3000 was added ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were transferred to an Imobilon-PPVDF membrane (Millipore, Billerica, MA, IPVH00010). Membranes were probed with rabbit anti-chick RUNX2 primary antibody (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich) and then purified with Superdex 200 pg (GE Healthcare) ...
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bioRxiv - Pathology 2022Quote: ... Target proteins were detected using ECL western blot detection solution (Millipore, WBKLS0500). Primary antibodies used for immunoblotting were as follows ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore), which were subsequently blocked with 5% blotting grade blocker (cat# 170-6404 ...
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bioRxiv - Neuroscience 2021Quote: ... Guanine nucleotide-binding protein subunit alpha-11 (1:1,000; Millipore 05-855R), Guanine nucleotide-binding protein G(q ...
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bioRxiv - Neuroscience 2021Quote: ... we used the EZ-Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (Millipore) following the manual with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich), using BSA as a standard and aliquots maintained at −80°C until required ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263, Merck Millipore; 1:1000). Ponceau solution was prepared with Ponceau BS (cat# B6008 ...
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bioRxiv - Molecular Biology 2021Quote: ... The separated proteins were transferred onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) using phosphate based transfer buffer (10 mM sodium phosphate monobasic ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted protein was then precipitated using 100% trichloroacetic acid (Sigma-Aldrich) added drop wise to 20% of the final elution followed by vortexing and incubation at 4°C overnight prior to pelleting at 21130 x g for 20 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: p53 and E2C proteins were labeled with fluorescein isothiocyanate (FITC) (Sigma-Aldrich), adapting the manufacturer’s protocol to obtain sub-stochiometric labeling enough to visualize the samples by fluorescence and confocal microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma, F7252) and imaged on ImageQuant LAS 4000 or Amersham ImageQuant 800 (GE Healthcare Life Sciences ...
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bioRxiv - Genomics 2020Quote: ... Protein loading was checked by staining membranes with Ponceau staining solution (Sigma) for 2min at room temperature ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Genomics 2020Quote: ... Cells were pelleted in Eppendorf® protein LoBind tubes (Sigma Aldrich Z666505) washed twice with ice cold PBS and snap-frozen ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were transferred to 0.2 μm pore-size nitrocellulose membranes (Millipore) and blocked in PBS with 0.1% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... All protein constructs were expressed in Escherichia coli BL21 (DE3) cells (Novagen) with a Tobacco Etch Virus (TEV ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The protein yield was estimated using the Bradford assay (Sigma-Aldrich, USA) with BSA as the standard ...
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bioRxiv - Biophysics 2020Quote: ... polyclonal antibody against brain lipid binding protein (BLBP) (Millipore, Merck KGaA, Germany), mouse antibody against nestin (Millipore ...
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bioRxiv - Developmental Biology 2019Quote: Protein was extracted using 1X RIPA lysis buffer (Millipore Sigma, Burlington MA) supplemented with Halt protease inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... immunoreactive proteins were detected by an enhanced chemiluminescence detection system (EMD-Millipore).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The separated proteins were then transferred to PVDF membranes (0.45μm, Millipore, USA), and block with 5% milk for 1h ...
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bioRxiv - Immunology 2019Quote: ... The protein bands were visualized by Immobilon Western Chemiluminescent HRP Substrate (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using a microplate Bradford (Sigma –Aldrich Bradford Reagent) protein assay with bovine serum albumin standards.
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bioRxiv - Cell Biology 2020Quote: ... we use the PureProteome Protein G Magnetic Bead System (EMD Millipore Corp.). 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996 ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was measured using the bicinchoninic acid assay (BCA) kit (Sigma).
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bioRxiv - Biochemistry 2019Quote: ... the reconstituted protein was mixed with 50 μM ADP-Ribose (Sigma-Aldrich) and further purified on a Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were visualized by staining with Amido Black staining solution (Sigma-Aldrich) according to manufacturer’s directions.