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Citations for Millipore Sigma :
1901 - 1950 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was determined by the Bradford method (Sigma-Aldrich, B6916). 50 μL magnetic beads (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using Amicon Ultra 3K MWCO filters (Millipore) in storage buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... Protein expression was performed in Bl21 (DE3) pLys competent cells (Novagen) using an L.B ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were also lysed in BugBuster Protein Extraction buffer (EMD Millipore) for 30 min instead of sonication.
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bioRxiv - Biochemistry 2023Quote: ... 25 µg of protein was reduced with 2 mM dithiothreitol (Sigma) for 60 minutes at 55 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to a methanol-activated PVDF membrane (Millipore) for 1 h at 100 V ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Cell cycle control protein 50A (anti-CDC50A/TMEM30A, Sigma, AV47410), anti-Microtubule-associated protein 1 light chain B (anti-MAPLC3/LC3 ...
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bioRxiv - Physiology 2023Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore; #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were detected using the Immobilon Western Chemiluminescent HRP Substrate (Millipore) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... injection of 40 μg of OVA protein (Grade V; Sigma-Aldrich) adsorbed to 2 mg of aluminum hydroxide (alum ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration was then measured using a Bradford assay (#B6916, Sigma), after which the proteins were diluted in Promega buffer (50 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were then concentrated using Amicon 3-kDa concentrators (Millipore), and the purity of each protein was assessed by SDS-PAGE and further confirmed by Western blotting using commercial antibodies against the strep tag (Biobharati Life Sciences ...
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bioRxiv - Physiology 2023Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Cenexin rabbit antibody (Protein tech) and centrin mouse antibody (EMD Millipore) diluted 1:1000 for centrin and 1:200 for other centrosomal proteins in 1% BSA in PBS for 1.5 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred from the gel onto PVDF membranes (EMD Millipore) using standard methods ...
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bioRxiv - Plant Biology 2024Quote: ... total proteins were detected with anti–HA–peroxidase (Sigma, clone 3F10), anti–FLAG (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... was immobilised on MagnaChIP™ Protein A magnetic beads (Sigma-Aldrich). Both the wildtype as well as the med25-1 mutant were used for the analysis under normoxic and hypoxic conditions ...
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bioRxiv - Immunology 2023Quote: ... Proteins were transferred to an Immobilon-FL membrane (Sigma Aldrich, USA) using a humid transfer device and 350 mA ...
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bioRxiv - Physiology 2024Quote: ... Protein content was determined using a Bradford assay (Sigma-Aldrich, USA).
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bioRxiv - Genetics 2023Quote: ... PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich) and separated by SDS-PAGE ...
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bioRxiv - Plant Biology 2024Quote: ... Rubisco was labelled using an Atto594 protein labelling kit (Sigma-Aldrich), according to manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... The protein concentration was measured using a Bradford Reagent (#B6916; Sigma). Protein samples (40μg ...
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bioRxiv - Genomics 2024Quote: ... and proteins were stained with Coomassie Brilliant Blue G-250 (Sigma).
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bioRxiv - Genetics 2024Quote: ... the proteins were transferred onto a PVDF membrane (Immobilon-P, Millipore) and blocked for 30 min at room temperature using intercept blocking buffer (LI-COR) ...
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bioRxiv - Developmental Biology 2024Quote: ... MPM-2 Mitotic Protein Monoclonal mouse 1:100 (Millipore 05-368), Lamin mouse 1:300 (DSHB ADL67.10) ...
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bioRxiv - Developmental Biology 2024Quote: ... The PROX1-FLAG protein was then purified on M2 agarose (Sigma), which captures FLAG-tagged proteins as described previously 52 ...
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bioRxiv - Evolutionary Biology 2024Quote: All recombinant proteins were expressed using pET-11a (Sigma #69436-3) based vectors in BL21(DE3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Separated proteins were transferred onto a PVDF membrane (IPVH00010, Millipore Sigma) by wet-protein transfer ...
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bioRxiv - Cell Biology 2024Quote: Proteins were extracted from cell pellets resuspended in RIPA buffer (Sigma) containing cOmpleteTM Mini Protease Inhibitor Cocktail (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were removed by phenol:chloroform:isoamyl alcohol (25:24:1) (PCI) (Sigma) extraction followed by ethanol precipitation ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were eluted with 5μg/ml FLAG peptide (Sigma, F3290) in buffer and subsequently separated on 4-12% NuPAGE Novex Bis-Tris Minigels (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred onto Immobilon-FL membrane (Millipore Cat. #IPFL85R) using transfer buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a PVDF membrane (Millipore Immobilion-P, IPVH00010) using a wet transfer with freshly made transfer buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... For labeling an Atto 488 protein labeling kit (Sigma-Aldrich, 38371) and an Atto 647N protein labeling kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated on Amicon Ultracel 10K centrifugal filters (Millipore) to 23 mg/ml concentration.
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bioRxiv - Neuroscience 2024Quote: ... Protein fragments were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen) cells ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were denatured with 5 % v/v 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in gradient Bolt Bis-Tris gels (Thermo) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced with isopropyl-b-D-thiogalactopyranoside (IPTG, Millipore) at 100 uM and cells were grown overnight at 16°C ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were harvested with RIPA lysis buffer (Sigma Aldrich; R0278) containing protease inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: Proteins were isolated from the liver using CelLyticTM MT (Sigma, C3228) in the presence of a protease inhibitor/phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred to Immobilon-P PVSF (0.45µm, Sigma-Millipore) in Tris-Glycine buffer supplemented with 20% methanol for 1 h at 75V ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred to Immobilon-P PVSF (0.45µm, Sigma-Millipore) in Tris-Glycine buffer supplemented with 20% methanol for 1 h at 75V ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to Immobilon-FL PVDF membrane (Merck Millipore, IPFL00010) at 100 volts using 1x Towbin transfer buffer with 20% methanol ...
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bioRxiv - Molecular Biology 2024Quote: ... and Etanercept chimeric proteins (Millipore Sigma Cat No. 185243-69-0), representing the extracellular domain of the target receptor fused to the Fc region of immunoglobulin G ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were transferred to a membrane (Immobilon-FL PVDF, IPFL00010, Millipore), stained with Revert 700 Total Protein Stain (926-11011 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Merk-Millipore, IPFL00010), blocked with SuperBlock Blocking Buffer in PBS (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the FLAG interacting protein mix was eluted by 3xFLAG peptides (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to polyvinylidene fluoride membrane (Millipore, Cat# ISEQ00010) following by incubation with blocking buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Equal protein loading was confirmed using Ponceau S Staining (P7170, Sigma). Blots were stained using indicated antibodies (Supplementary Table 2) ...
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bioRxiv - Pathology 2024Quote: ... Transfer the proteins onto a PVDF membrane (ISEQ00010, Merck Millipore, Germany). Block the membrane with 5% skim milk for 30 minutes ...