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Citations for Millipore Sigma :
151 - 200 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and all cells were tested and found to be Mycoplasma-free using the LookOut PCR Detection Kit (Sigma).
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bioRxiv - Biochemistry 2020Quote: ... Venor GeM PCR-based mycoplasma detection kit was used as stated in the manual and was from Sigma. ECL substrate and ECL-Plus substrate were from Pierce and were used as directed ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were monitored for mycoplasma using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich, cat. MP0035). THP-1 positive controls for phospho-STING (S366 ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were tested to be free of mycoplasma contamination using a mycoplasma PCR detection kit (Sigma). Cell lines were authenticated using short tandem repeat (STR ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell cultures were routinely checked for mycoplasma using the LookOut® Mycoplasma PCR Detection Kit (Sigma Aldrich, #D9307).
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were tested for mycoplasma contamination using the LookOut® Mycoplasma PCR Detection Kit (Sigma Millipore) and were found to be negative for mycoplasma.
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were tested for mycoplasma contamination using the LookOut® Mycoplasma PCR Detection Kit (Sigma Millipore) and were found to be negative for mycoplasma.
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bioRxiv - Microbiology 2024Quote: ... Each cell line was tested monthly for mycoplasma using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich, # MP0035) and confirmed mycoplasma free ...
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bioRxiv - Microbiology 2024Quote: ... Cells were inspected for mycoplasma contamination using a VenorTM GeM Mycoplasma Detection kit PCR-based (Sigma, MP0025-1KT).
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bioRxiv - Cancer Biology 2023Quote: ... cells were confirmed to be negative for mycoplasma using LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich, MP0035).
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bioRxiv - Cancer Biology 2023Quote: ... Mycoplasma testing was routinely performed using the LookOut® Mycoplasma PCR Detection Kit (Sigma, MA, USA, MP0035-1KT) to ensure the absence of contamination before cells were used in experiments.
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bioRxiv - Biochemistry 2023Quote: ... Jurkat were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Microbiology 2023Quote: ... Cells were screened negative for mycoplasma contamination by monthly scheduled testing (LookOut® Mycoplasma PCR Detection Kit, Sigma). Stable cell lines expressing Sm.TRPMPZQ or Fh.TRPMPZQ were generated using the Flp-In T-REX core kit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... We confirmed the absence of mycoplasma contamination in cells using the LookOut Mycoplasma PCR Detection Kit (Millipore Sigma).
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bioRxiv - Biochemistry 2024Quote: ... Cells were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Slides were washed with SSC buffer (S6639, Sigma), followed by staining with DAPI (P36935 ...
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bioRxiv - Neuroscience 2022Quote: ... in 2X Sodium Sodium-Citrate buffer (SSC, Sigma) for Mouse 448 and human experiments or 1 mg/ml Hoechst (Sigma ...
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bioRxiv - Genomics 2020Quote: ... 20× saline sodium citrate (SSC, Sigma-Aldrich, S6639), 50% OmniPur Dextran Sulfate (EMD Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... in 2x saline-sodium citrate (SSC) (Sigma-Aldrich) buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 2x saline-sodium citrate (SSC; #S6639, Sigma-Aldrich), 1 µg/µl BSA (#R396A ...
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bioRxiv - Physiology 2023Quote: ... 5x saline-sodium citrate (SSC, Sigma, 8310-OP), and 50% (v/v ...
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bioRxiv - Genetics 2024Quote: ... and two washes with 2X SSC (Sigma Aldrich) and the hybridization ...
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bioRxiv - Neuroscience 2022Quote: Digoxygenin (DIG)-labeled probes were produced with the SuperScriptTM III One-Step RT-PCR kit (Sigma 12574-026) using specific primers for the Bgl-FaNaC transcript (Forward ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time qPCR was performed using SYBR Green JumpStart Taq ReadyMix (Sigma) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Real-time qPCR was performed with the following probes from Sigma Aldrich: LOX fw ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative RT-PCR was performed using Kapa SYBR Fast (Sigma). SREBP genes primer sequences are listed in Supplementary Table S3 ...
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bioRxiv - Molecular Biology 2020Quote: RT-PCR: 106 cells were lysed in TRI reagent (Sigma) and RNA extracted using Direct-zol columns with a 30 minute on-column DNase step at room temperature (Zymo Research) ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-PCR was performed using REDTaq® ReadyMix™ (Sigma) on a thermocycler (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... Samples for quantitative PCR and quantitative RT-PCR were homogenized in TRiZOL reagent (Sigma Aldrich) and further processed for nucleic acid extractions using manufacturer’s protocols.
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bioRxiv - Genomics 2020Quote: ... All iPSCs and cerebral organoids were tested negative for mycoplasma using the LookOut Mycoplasma PCR Detection kit (Sigma MP0035). iPSCs from all donors were passaged until they were confluent ...
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma contamination check was carried out using a LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich, St. Louis, MO, USA). Cells were validated by STR profiling.
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bioRxiv - Cell Biology 2021Quote: ... Cell cultures were examined periodically for bacteria contamination and tested for mycoplasma by LookOut Mycoplasma PCR Detection Kit (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were tested for mycoplasma every three months using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) and were authenticated by ATCC®.
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were regularly tested for mycoplasma contamination using a LookOut® Mycoplasma PCR Detection kit (Sigma, MP0036).
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bioRxiv - Developmental Biology 2023Quote: ... Both hiPSC lines were checked for mycoplasma contamination and tested negative (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich MP0035).
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA extracted from parasite cultures was regularly tested for Mycoplasma contamination (look out Mycoplasm PCR detection kit (Sigma)) using MSP primers 44 All transgenic P ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-QPCR was performed in triplicate for each samples using the KAPA SYBR PCR kit (Sigma Aldrich KK4600) with a LightCycler 480 apparatus (Roche) ...
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bioRxiv - Developmental Biology 2024Quote: ... The total isolated mRNA was reverse transcribed using First Strand cDNA Synthesis Kit for RT-PCR (Sigma Aldrich 11483188001). RT-PCRs were conducted using Taq polymerase (Invitrogen EP0401) ...
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bioRxiv - Immunology 2023Quote: ... quantified using LDH Cytotoxicity Detection Kit (Sigma) according to manufacturer directions ...
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bioRxiv - Cancer Biology 2020Quote: ... After probe hybridization increasingly stringent washing steps were applied in saline-sodium citrate (SSC) buffer (Sigma, SSC 20x buffer concentrate S6639-1L) at various concentrations and temperatures ...
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bioRxiv - Genomics 2021Quote: ... Coverslips were then rinsed in 2X-SSC (1X SSC: 150mM sodium chloride, 15mM sodium citrate) 3x and incubated for >24 hrs in 50% formamide (Sigma, catalog # 47671) / 2X-SSC (pH 7.0 ...
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bioRxiv - Bioengineering 2022Quote: ... Real-Time qPCR was performed using the Fast SYBR Green Master Mix (Sigma) in a QuantStudio 5 Real-Time-PCR-Cycler (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed twice with 2x SSC (Sigma-Aldrich) for 5 min then permeabilized with 0.2 M HCl + 0.7% Triton X-100 for 10 – 15 min at RT ...
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bioRxiv - Biophysics 2020Quote: ... 5% CO2 tissue culture incubators and routinely confirmed to be mycoplasma negative using LookOut mycoplasma PCR detection kit (Sigma Aldrich). pLifeAct-mScarlet-N1 and pLck-mVenus-C1 were gifts from Dorus Gadella (Addgene plasmids #85054 and #84337 ...
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bioRxiv - Microbiology 2020Quote: ... validated as hex1 deletion mutants by spore PCR and Southern blot analysis (DIG DNA Labeling and Detection Kit, Sigma-Aldrich), and stored as 25% glycerol stocks at −80 °C.
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were routinely tested for mycoplasma contamination on a monthly basis using the LookOut Mycoplasma PCR Detection Kit (Sigma). Any cells yielding positive mycoplasma results were immediately discarded.
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bioRxiv - Microbiology 2022Quote: ... trachomatis serovar L2 stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO).
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bioRxiv - Cancer Biology 2020Quote: The cell-lines were confirmed to be mycoplasma negative using the Venor™GeM Mycoplasma Detection PCR kit (MP0025; Sigma).
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bioRxiv - Microbiology 2019Quote: ... All cell lines were regularly tested for mycoplasma contaminations using LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich, MP0035-1KT). Additionally ChIP-seq reads from input samples were aligned to mycoplasma reference sequences to exclude contamination.
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bioRxiv - Neuroscience 2022Quote: Mycoplasma testing was performed monthly for all the lines included in this study at any differentiation stage (LookOut Mycoplasma PCR Detection Kit, Sigma). All cells used in this study were consistently negative.