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Citations for Millipore Sigma :
101 - 150 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... and cells were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich). Infectious stocks of a cell culture-adapted strain of genotype 2A JFH1 HCV were generated and titered in Huh-7.5 cells by focus-forming assay (FFA) ...
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bioRxiv - Molecular Biology 2019Quote: ... H69 cells were Mycoplasma free as established using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) and authenticated using STR profiling by ATCC before the start of this study (not shown).
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were tested every fortnight for Mycoplasma using LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich). Cell line identity was confirmed using STR profiling.
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bioRxiv - Cancer Biology 2020Quote: ... All cultures were routinely tested for mycoplasma contamination using the LookOut Mycoplasma PCR Detection kit (Sigma). To induce differentiation ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were routinely tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich). Cell lines were authenticated by ATCC with no further authentication carried out after purchase.
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bioRxiv - Microbiology 2020Quote: ... The cell line was tested free of mycoplasma contamination using LookOut Mycoplasma PCR Detection Kit (SIGMA). Cultured cell monolayers were maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... All cell lines tested negative for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... All cell lines were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... The cells were tested for mycoplasma using the LookOut® Mycoplasma PCR Detection Kit (Sigma, # MP0035), following the manufacturer’s instructions and using the JumpStart™ Taq DNA Polymerase (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Cell lines were tested for mycoplasma using a PCR assay (Venor GeM Mycoplasma Detection Kit, Sigma).
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bioRxiv - Microbiology 2024Quote: ... All cell lines were confirmed mycoplasma free using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... Cells were continuously verified as mycoplasma free using the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma, MP0035), using JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were routinely tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma Aldrich).
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bioRxiv - Physiology 2021Quote: ... ROS detection kit (D6883, Sigma) containing 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and chemiluminescence detection kit (Millipore) were used for visualization with ChemiDoc Touch Imaging System (BioRad) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded on 96-well PCR plate and run in CFX real-time PCR System (Bio rad) using KiCStart SYBR Green qPCR ReadyMix with ROX as recommended by the manufacturer (Sigma-Aldrich: KCQS02). Three technical replicates were performed for each sample together with no-template control (NTC ...
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bioRxiv - Genomics 2019Quote: ... Real time PCR was carried out in a Stratagene MX3005P thermocycler using SYBR Green Jump Start Taq Ready Mix (Sigma Aldrich, USA) for quantifying amplification results ...
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bioRxiv - Cancer Biology 2021Quote: ... The real-time PCR analysis was also performed with the Complementary DNAs using JumpStart Taq DNA polymerase (Sigma-Aldrich, St. Louis, MO) and SYBR green nucleic acid stain (Molecular Probes ...
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bioRxiv - Cancer Biology 2021Quote: ... After washing by 0.1*SSC (Millipore Sigma), RT Master Mix (provided in Visium Reagent kit ...
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bioRxiv - Developmental Biology 2022Quote: ... in 0.5X SSC containing 5% formamide (Sigma) for 2-3 h under light ...
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bioRxiv - Neuroscience 2023Quote: ... into 2x SSC Buffer (Sigma-Aldrich, #AM9770) containing hybridization capture probe at 25 nM ...
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bioRxiv - Neuroscience 2023Quote: ... into 2x SSC Buffer (Sigma-Aldrich, #AM9770) containing hybridization probes at 25 nM and recombinant proteinase K enzyme (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... confirmed by the use of Mycoplasma PCR Detection (Sigma) were regularly passaged to maintain exponential growth and used from early passages (<10 passages after thawing) ...
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bioRxiv - Genetics 2021Quote: ... cells tested for several strains of mycoplasma contamination using LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich #D9307).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were routinely tested for mycoplasma contamination using the Lookout Mycoplasma PCR Detection Kit (#MP0035; Sigma-Aldrich). Cells were treated with the proteasome inhibitor MG132 (ApexBio ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were tested periodically for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit from Millipore Sigma.
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bioRxiv - Immunology 2024Quote: ... Cell lines were confirmed to be mycoplasma-free with the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich). Genotyping of various mice used in this work was accomplished with the APExBIO direct mouse genotyping kit and primers described in Table S1 ...
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bioRxiv - Genomics 2023Quote: ... Cells were routinely tested for free of mycoplasma by using LookOut Mycoplasma PCR detection kit (Sigma, #MP0035).
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for the mycoplasma absence using the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... All cells were examined negative for mycoplasma infection using PCR-based Mycoplasma Detection Kit (Sigma, MP0035-1KT). Mouse embryonic fibroblast cell (MEF ...
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bioRxiv - Microbiology 2023Quote: ... All the cell types were confirmed free of mycoplasma using Lookout Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (cat#MP0035, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: Cells in culture were routinely tested for mycoplasma infection with Lookout Mycoplasma PCR detection kit (Sigma, MP0035), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines were verified as mycoplasma free using the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich) and genetic identities were validated by PCR ...
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bioRxiv - Microbiology 2020Quote: We performed reverse transcription using the First strand cDNA synthesis kit for RT-PCR (AMV) (Sigma-Aldrich) and the protocol described by the supplier ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PRC using cDNA was performed using KAPA SYBR fast qPCR master mix kit (Sigma Aldrich, USA) and Applied Biosystems StepOne Plus Real-Time PCR system ...
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bioRxiv - Microbiology 2020Quote: ... Post-hybridization cell pellets were washed in wash buffer twice, then washed in 2X SSC before being resuspended in anti-fade buffer (2X SSC, 0.4% glucose (Sigma), Tris-HCL pH 8.0 (USB Corporation ...
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bioRxiv - Genetics 2022Quote: ... or miR-27b-3p mimics (Sigma) for 48 h ...
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bioRxiv - Microbiology 2022Quote: ... Cells were verified using the Promega GenePrint STR kit (DNA Analysis Facility, Duke University) and as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... Chemiluminescence was generated by ImmunoStar detection kit (Wako) or Immobilon (Millipore) and detected by LAS-3000 mini system (FUJIFILM).
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bioRxiv - Genetics 2020Quote: Cell Growth Detection Kit (MTT) (Sigma) was used for detecting cell proliferation ...
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bioRxiv - Neuroscience 2022Quote: ... followed by detection using standard ECL chemi-luminescence detection kit (Millipore, WBKLS0500).
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bioRxiv - Microbiology 2024Quote: ... Signal detection was carried with DIG luminescence detection kit (Sigma Aldrich, #11363514910) following manufacturers instruction and signal captured with ChemiDoc (Biorad) ...
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bioRxiv - Genomics 2022Quote: ... 75μl of a mix of the 16S probe (5’-GGATTAGATACCCBDGTAGTCGAGATNBAAAAAAAAAAAAAAAAAAAA-3’, IDT, USA) at 0.6μM concentration with 4X Saline Sodium Citrate (SSC) (SSC, Sigma-Aldrich, USA) and 0.1% SDS (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... The probe mix was then removed and the wells were washed with 100μl of 0.1X SSC (SSC, Sigma-Aldrich, USA). To covalently attach the now hybridized 16S probes onto the surface probes ...
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bioRxiv - Cell Biology 2024Quote: ... in which the 2x SSC buffer (300 mM NaCl, 30 mM sodium citrate, pH = 7.0) was diluted from 20x SSC (Sigma S6639) using UltraPure DNase/RNase-Free Distilled Water (Thermo 10977015) ...
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bioRxiv - Biochemistry 2019Quote: ... catalog number G1112 and an electron coupling reagent (PMS) [CAS: 299-11-6] from Sigma (St. Louis, MO,USA).
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bioRxiv - Systems Biology 2021Quote: Steroidogenic fetal adrenal cells were incubated in cultivation medium in each well with or without 100 nmol/l SST (hor-299-a, ProSprc-Tany) and 0.1mmol/l cholesterol (C3045, Sigma) intervention for 24 h(Damsteegt ...