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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Bound protein was eluted in 30 μl of elution buffer (0.15 mg/ml 3xFLAG peptide [Sigma, St Louis ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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bioRxiv - Genomics 2020Quote: ... a saturation step was performed with blocking solution (blocking reagent Sigma #11096176001 ...
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bioRxiv - Immunology 2022Quote: ... After blocking with IHC Select Blocking Reagent (Millipore, cat. 20773-M) at RT for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to nitrocellulose membranes before blocking with 5% milk (Marvel) or bovine serum albumin (Sigma) w/v in PBS-T (0.1% Tween-20 in PBS) ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides desalting was carried out using ZipTip U-C 18 spin columns (Cat. No. ZTC18M096, Millipore Corporation U.S.A.). The peptides were dried in a speed vac and then reconstituted with 0.1%Formic acid in water.
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bioRxiv - Cell Biology 2023Quote: ... Peptides contained a C-term PEG-Biotin and were synthesized using a preloaded PEG-Biotin resin (Sigma-Aldrich). Peptides were cleaved from the resin using TFA / Water / Thioanisole / Triisopropylsilane / Phenol / Ethanedithiol – 82.5/0.5/0.5/0.25/0.25/0.25 and precipitated into cold diethyl ether ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Cancer Biology 2019Quote: ... then incubated with primary antibody overnight at 4°C in Odyssey Blocking Buffer with 0.1% Tween-20 (Sigma-Aldrich). Membranes were then probed with corresponding Li-Cor anti-mouse or anti-rabbit fluorescent secondary antibodies for one hour at room temperature at dilutions of 1/10 000 in Odyssey Blocking Buffer with 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Cells were blocked overnight at 4°C with 150µl blocking solution per well (5% (v/v) human serum (Sigma), 5% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then incubated overnight at 4°C with primary antibody diluted in blocking solution (rabbit anti-FLAG, Sigma, 1:250 or mouse anti-Tuj1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then washed with 1xPBS and blocked for 30 minutes at 37°C in blocking buffer (1xPBS, 0.05% BSA [Invitrogen, Am2616], 50μg/mL yeast tRNA [Sigma-Aldrich, R8759] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then incubated overnight at 4°C with primary antibodies diluted in blocking solution [5% Goat Serum (Sigma Aldrich) + 0.2% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... incubated overnight at 4°C in blocking buffer with primary antibody (1:500 mouse anti-Elastin, Millipore Sigma MAB2503), washed ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody was applied overnight at 4°C in blocking buffer (α-Actinin, Sigma A7811, 1:2500; AURKB, Sigma A5102 ...
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bioRxiv - Biophysics 2023Quote: ... cells in suspension were incubated for 20 minutes at 37°c in the blocking antibody (integrin α5β1, clone JBS5, Sigma) or control antibody (IgG ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reactions were performed with the Readymix Red Taq PCR reactive mix (Sigma-Aldrich), Phusion high-fidelity DNA polymerase (Fermentas ...
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bioRxiv - Microbiology 2019Quote: ... PCR reactions were performed with the Readymix Red Taq PCR reactive mix (Sigma-Aldrich) or with the Phusion high-fidelity DNA polymerase (Fermentas ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were labeled with a photo-reactive and clickable cholesterol analog (pacChol) (Sigma-Aldrich) with a final concentration of 6 µM for 30 min at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG peptide (Sigma) was applied to the column to elute the FLAG protein complex according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... SIINFEKL peptide (Sigma), and human CD4-Fc fusion protein (generated in-house at Genentech ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG peptide (Sigma) was applied to elute the Flag-labeled protein complex as described by the vendor ...
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted from magnetic beads with 1 mg/ml of 3×FLAG peptide (Sigma F4799) with agitation at 4 °C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed three times and FLAG-tagged proteins eluted using 3X FLAG peptide (Sigma; cat. F4799). Rhodamine-Azide labeling reactions were performed using 1.7 mM TBTA ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were eluted twice with 2 ug/uL V5 peptide in water (Sigma-Aldrich, St. Louis, MO) at 37°C for 15 minutes with shaking at 1000rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound proteins were eluted using IP buffer containing 0.25 mg/mL of 3x FLAG peptides (Sigma-Aldrich) at 25 °C for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were eluted by the addition of elution buffer containing 3 x Flag peptide (Sigma, cat. #F4799) at 4°C for 30 min ...
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bioRxiv - Systems Biology 2021Quote: ... Yeast proteins digestion was performed on excised bands from gel gradient and digested peptides of UPS2 (Sigma) were used as external standards for absolute protein quantification (more details in Supp ...
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bioRxiv - Cell Biology 2021Quote: ... the proteins bound to the beads were eluted by the addition of FLAG peptide (#F3290, Sigma-Aldrich). Proteins in the eluate were separated by SDS-PAGE to detect Tanmp-interacting proteins by Western blotting or by silver staining ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitated proteins were eluted with 0.5 mg/mL (diluted in lysis buffer) 3X FLAG peptide (Sigma, F4799) for 30 minutes on a rotator ...
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bioRxiv - Biochemistry 2022Quote: ... bound proteins were eluted with lysis buffer containing 300 μg/ml Flag peptide (Sigma-Aldrich, Cat# F3290) at 4 °C for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The ORF1p-FLAG protein complex bound was eluted using 200 μg/mL of 3xFLAG peptide (Sigma-Aldrich) in the Lysis150 buffer containing 0.2 mM PMSF and 1x cOmplete EDTA-free protease inhibitor cocktail by incubation at 4°C for 1 hour with gentle rotation ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein elution was carried out under native conditions by competition with commercially available 3× FLAG peptide (Sigma). Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant protein was then eluted in lysis buffer containing 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich) for 1 hr with rotation at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were eluted twice (15 min. at RT) by incubation with 3x-FLAG peptide (Sigma #F4799) at a concentration of 100 mg/mL in PBS buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-surfactant associated protein C (SFTPC, 1:1000, Millipore, AB3786), goat anti-podoplanin (PDPN ...
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bioRxiv - Developmental Biology 2022Quote: ... polyclonal rabbit anti-pro surfactant protein C (1:4000, Millipore, #AB3786), mouse monoclonal acetylated tubulin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal Prosurfactant Protein C (1:200 pH9, AB3786, Merck Millipore), mouse monoclonal TTF-1 (clone SPT24 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Microbiology 2020Quote: ... the peptide corresponding to the C-terminus region of BtrS (CALREALRERGYDSVP) were conjugated with hemocyanin from keyhole limpets (Sigma) by using 3-maleimidobenzoic acid N-hydroxysuccinimide ester (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore) coated with AN18 IFNγ (1 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged MSL proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Elution buffer ...
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bioRxiv - Biochemistry 2023Quote: ... with molar ratio of 1:5 (Nucleosome:SUV420H1) or 1:100 (Nucleosome: SUV420H1 C-terminal peptide) in the presence of 40 µM of SAM (Sigma). Samples were incubated on ice for 20 mins ...
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bioRxiv - Molecular Biology 2019Quote: ... Bound peptides were eluted with 400 μg/ml Flag peptide (Sigma) in BC100 buffer for 20 min on ice.
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C in blocking buffer with the following primary antibodies: rat anti-MBP (monoclonal, 1:100, Millipore), rabbit anti-Parvalbumin (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2 ...