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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and the peptide mixtures were desalted on C-18 reverse phase material (ZipTip μ-C18, Millipore). Eluted peptides were concentrated by evaporation under vacuum and subsequently resolved in 0.1% acetic acid / 2% acetonitrile containing HRM/iRT peptides (Biognosys ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was performed in Millipore blocking reagent (EMD Millipore), followed by incubating the section in Anti-GFP (ab13970 ...
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bioRxiv - Microbiology 2023Quote: ... followed by blocking with 1x casein blocking buffer (Sigma) for 4 hours at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... any remaining sulfhydryl-reactive handles were quenched with L-cysteine (Sigma Aldrich) by reacting for at least 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4 C in blocking solution containing rabbit anti-NeuN (Millipore, ABN78) primary antibody at 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated at 4°C in blocking solution with either 1:200 SOM antibody (MAB354, Millipore) or 1:5000 anti-DDDDK tag (anti-FLAG Tag to label smFP ...
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bioRxiv - Neuroscience 2020Quote: ... at 4°C in the same blocking buffer: rabbit anti-PMP22 (Sigma-Aldrich, SAB4502217, 1/750 dilution), mouse anti-β-Actin Clone AC-15 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated at 4°C in blocking solution with BODIPY membrane dye (Sigma, D3821, 1:1000) for 24 hours ...
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bioRxiv - Physiology 2023Quote: ... followed by overnight incubation at 4°C in blocking buffer containing mouse anti-vimentin antibody (Sigma, MAB3400) diluted 1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-protein kinase C-α (PKCα) (Sigma, P4334); rabbit anti-VSX2 (Chx10 ...
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bioRxiv - Microbiology 2020Quote: ... bound proteins from the pooled washed resin were eluted with 100μg of FLAG peptide (Sigma) in FLAG wash buffer ...
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bioRxiv - Biochemistry 2019Quote: ... The native proteins without signal peptides were expressed in BL21(DE3) Gold competent cells (Novagen). The gene encoding the LtgA deletion mutant lacking the alpha helix 503(ATAREIAGKIGMD)513 was chemically synthesized by ProteoGenix ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Microbiology 2020Quote: ... the sequence encoding mature proteins without the signal peptide was cloned into pET-14b (+) (Novagen) to generate the expression vector pET14b-6xHis-BcCrh1 ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins were eluted two times with 600 μL 250ug/mL 3xFLAG peptides (SIGMA, F4799) in TBS [50mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Biophysics 2020Quote: ... Protein was eluted with Myosin Buffer supplemented with 100 μg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to Storage Buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... and the ATAD5-RLC proteins were eluted with 100 µg/mL FLAG-peptide (Sigma Aldrich) in the same buffer ...
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bioRxiv - Biophysics 2021Quote: Proteins and peptides were labelled at their N-terminus with Fluorescein 5-Isothiocyanate (FITC, Sigma), purified and quantified following a described protocol [45] ...
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bioRxiv - Cell Biology 2020Quote: ... The bound protein was eluted four times with 250 μL of HA peptide (Sigma-Aldrich) (0,1 mg/ml).
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound proteins were neutrally eluted with 50 µL of V5 peptide (V7754-4MG, SIGMA) at 1mg/mL in PBS ...
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bioRxiv - Immunology 2022Quote: ... Bound proteins were eluted by incubating the beads with 200µg/ml 3xFLAG peptide (Sigma- Aldrich) in IPP150 containing 0.02% NP-40 ...
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bioRxiv - Biophysics 2023Quote: ... Protein was eluted with wash buffer supplemented with 100 mg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to a storage buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-proteins were eluted with 50 µL 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) at RT for 1h and supplemented with 5x SDS sample buffer (250 mM Tris-HCl pH 6.8 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted by incubation with 25 µL of 100 µg/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunoprecipitated overnight at 4 °C with 2 µg affinity-purified rabbit anti-PKM2 antibody raised against a C-terminal peptide (Sigma cat. # SAB4200105, Lot #030M4874) pre-coupled to Dynabeads sheep M-280 anti-rabbit IgG beads (LifeTech) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a PVDF membrane and incubated in 1% Western blocking reagent (Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... were isolated from either naïve or infected or tumour-bearing HHD or BALB/c mice and were cultured in the presence of HLA-A2 restricted IAV M1 peptide 158-66(GILGFVFTL)(Cambridge peptide) or NY-ESO-1 peptide 157-65 (SLLMWITQC)(Sigma peptide) or H-2Kd binding IAV NP peptide 147-55(TYQRTRALV ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 1 h and eluted with 0.1 mg/mL 3X FLAG peptide (Sigma-Aldrich). E-buffer (20 mM Na·HEPES pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... and eluted with 5 mL of buffer C containing 0.1 mg/mL FLAG peptide (Sigma-Aldrich, F3290). BLM was further purified using a 1 mL HiTrap SP (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... and eluted with 5 mL of buffer C containing 0.1 mg/mL FLAG peptide (Sigma-Aldrich, F3290). DNA2 was further purified using a 1 mL HiTrap SP (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated at 4°C in blocking solution with Hoechst 33342 nuclear dye (Sigma, H21492, 1:2000) for 24 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Primary antibodies were applied overnight at 4°C in a blocking buffer of 5% donkey serum (Sigma-Aldrich) at the following concentrations ...
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bioRxiv - Cancer Biology 2019Quote: ... then antibody-reactive bands were visualized via enhanced chemiluminescence (ECL) system (Millipore, USA) in an Imager ...
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bioRxiv - Neuroscience 2022Quote: ... The immune-reactive bands were acquired using Immobilon Western Chemiluminescent HRP Substrate (Millipore) and visualized with the Fusion FX (Vilber Lourmat ...
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bioRxiv - Bioengineering 2023Quote: ... The immune-reactive bands were detected using an enhanced chemiluminescence kit (Sigma Aldrich) and ChemiDoc imaging system (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: ... RGD peptide or as control GRADSP (RAD) peptide (SIGMA) was injected via Wharton’s duct cannulation (approximately 600 nmol of either peptide in 30 µl per lobe in PBS) ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
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bioRxiv - Molecular Biology 2021Quote: ... except that elution of bound proteins was done using 100 μg/ml FLAG peptide (Sigma, F3290).44 In brief ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was eluted twice from resin using 100μL of 500ng/μL [3x flag peptide (Sigma F4799), Myc (Pierce 20170) ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Bound effectors and associated plant proteins were competitively eluted by 3XFLAG peptides (Sigma-Aldrich, Dorsett, England) and separated by SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... The protein complexes were specifically eluted with 125 mkl of 100 ng/mkl 3xFLAG peptide (Sigma) solution in TBS during 30 min on-ice incubation with agitation ...
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bioRxiv - Cell Biology 2019Quote: ... bound proteins were eluted using 100 μL of FLAG peptide (100 μg/mL, Sigma-Aldrich, F4799), and then with 100 μL of 2x Laemli buffer (62.5 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Absolute quantification of each protein is performed through applying AQUA™ Peptides purchased from Sigma-Aldrich. A summary of all quantification results were shown in S8 Table.
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were eluted twice with 50 ul of 1 mg/ml 3X flag peptide (Sigma) in co-IP buffer on the nutator for 10 minutes at room temperature.
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bioRxiv - Plant Biology 2022Quote: ... Captured proteins were eluted off FLAG beads using 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich). Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... bound proteins were eluted using buffer A containing 500ng/μL 3X FLAG® peptide (Sigma; F4799) for followed SDS-PAGE.
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bioRxiv - Cell Biology 2022Quote: ... and immunoprecipitated proteins were eluted from Dynabeads by incubating with 200 μM FLAG peptides (Sigma-Aldrich) for 90 min ...