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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... C-reactive protein (CRP) was measured by an ELISA kit (cat. number CYT294, Millipore Sigma, Saint Louis, MO) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: Human C-reactive protein (Cat. No. C4063) and Human erythrocyte catalase (Cat. No. C3556) were obtained from Sigma Aldrich chemicals ...
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
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bioRxiv - Physiology 2020Quote: ... and human C-peptide (Millipore) were detected by ELISA kits in the plasma samples collected during the human insulin or human C-peptide challenges ...
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bioRxiv - Biophysics 2020Quote: ... N-terminally labelled PR25 was obtained by reacting the peptide with amine-reactive AlexaFluor546 (Sigma-Aldrich). PolyU RNA with a molecular weight range from 800– 1,000 kDa was purchased from Sigma-Aldrich ...
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bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore, Billerica ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody-reactive proteins were detected by western chemiluminescent HRP substrate (ECL) (Millipore). Images were acquired by FluorChem FC3 image analyzer (Molecular Devices).
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bioRxiv - Plant Biology 2022Quote: ... Elution of immunoprecipitated proteins was performed using 100 μg/mL c-Myc peptide (Sigma, M2435), on ice for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Co-immunoprecipitated proteins were eluted for 1h at 4°C with 3X FLAG peptide (Sigma) in TBS (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoprecipitated proteins were eluted overnight at 4 °C with 50 μg of Flag peptide (Sigma-Aldrich) in 100 μL complemented non-denaturing lysis buffer ...
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bioRxiv - Genetics 2021Quote: ... The immune-reactive proteins were detected using the Luminata Crescendo Western HRP substrate (Millipore).
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bioRxiv - Microbiology 2019Quote: ... Reactive proteins were detected with either the HRP-conjugated goat anti-mouse IgG (Sigma) or donkey anti-rabbit IgG (1:3000 ...
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bioRxiv - Physiology 2019Quote: ... and C-peptide (Sigma Aldrich; reference #EZHCP-20K) concentrations were measured using commercially available ELISA kits (intra-assay CV for insulin ...
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bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
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bioRxiv - Cell Biology 2022Quote: ... Integrin-blocking (GRGDSP, #SCP0157) and negative control (GRADSP, #SCP0156) peptides were purchased from Sigma. Recombinant human integrins αvβ3 (3050-AV) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 uM Calpain Inhibitor Peptide (Sigma-Aldrich C-9181;); BSA was included to prevent non-specific binding ...
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bioRxiv - Cancer Biology 2020Quote: ... bound proteins were eluted with FLAG peptide (Sigma) and analyzed on Western blots as above.
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bioRxiv - Microbiology 2021Quote: ... Azo dye Reactive Black 5 (Sigma) was added to the cell suspension at a final concentration of 0.005% ...
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bioRxiv - Immunology 2023Quote: ... The protein was then eluted in 15 μL of peptide elution buffer three times using Flag peptide or HA peptide (Sigma) 300µg/mL ...
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bioRxiv - Pathology 2020Quote: ... 150 mM NaCl containing the corresponding detergents and bound proteins were eluted three times with 10 μL 0.5 mg/mL c-Myc peptide (Sigma M2435) in the corresponding detergent containing buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads-antibody-protein mix was incubated overnight at 4°C and bounds protein were eluted with 35 μg HA peptides diluted in PBS (Sigma); then Laemmli buffer was added and the eluate heated at 95°C 2 min.
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bioRxiv - Immunology 2023Quote: ... for 2h at 4°C and washed four times and precipitated protein were competitively eluted with HA-peptide (100ng/ml; I2149, Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... Immuno-reactive proteins were visualized using the Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA). The detected bands on the scanned blots were quantitated with ImageJ freeware.
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bioRxiv - Molecular Biology 2021Quote: ... Serum insulin and c-peptide levels were measured by insulin or C-peptide ELISA respectively following the manufacture’s protocol (Sigma Aldrich, St. Louis, MO, USA). Mouse metabolic testing and fasting methods followed established protocols [16,33,34].
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bioRxiv - Cell Biology 2019Quote: ... Human c- peptide ELISA kit (cat # EZHCP-20K, Millipore MA), Human Insulin ELISA kit (cat # ELH- Insulin ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with Flag peptide (Sigma Aldrich) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were competitively eluted using 3X FLAG peptide (Sigma; in 10 mM Tris HCl ...
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bioRxiv - Physiology 2020Quote: ... Mouse insulin and C-peptide were detected by multiplex ELISA (Millipore) kit in the plasma samples collected during the oral glucose challenge ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... #216763)/1X PBS for 15 minutes at 4 °C, followed by blocking with a blocking buffer (0.1 M Tris, pH 7.5, 100 mM maleic acid (Sigma-Aldrich, #M0375), 150 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein complexes were eluted with 3xFlag peptide (200ug/mL, Sigma) in 5 column volumes Buffer 2 for 1 hour at 4C ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were eluted using a 3xFLAG peptide (Sigma #F4799). Samples were then heated to 100°C for 10 minutes in SDS sample buffer and analyzed by western blotting.
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bioRxiv - Biochemistry 2022Quote: ... the bound proteins were eluted with 3X FLAG peptide (Sigma). The purity of DNA2 proteins was analyzed by 4–15% gradient SDS–polyacrylamide electrophoresis (SDS–PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799). Trem2 was immunoprecipitated with either a Rabbit monoclonal antibody raised against the carboxy-terminus of mouse Trem2 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein complexes were eluted with 3x-FLAG peptide (Sigma-Aldrich) or HA peptide (Thermo Fisher ...
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bioRxiv - Neuroscience 2019Quote: ... reactive astrocytes (GFAP, mouse; 1: 1000, Sigma, USA), microtubule associated protein 2 (MAP2 ...
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bioRxiv - Genetics 2020Quote: Cy3 Mono-Reactive NHS Ester (Millipore Sigma PA13105);
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bioRxiv - Genetics 2020Quote: Cy5 Mono-Reactive NHS Ester (Millipore Sigma PA15101);
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bioRxiv - Molecular Biology 2020Quote: ... followed by elution with 100 μg/ml c-Myc peptide (Sigma-Aldrich) on an end-to-end rocker at room temperature for 1 hr ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 4°C and eluted with the HA peptide (Sigma #I2149) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL of this dilution was then added to 90 μL of buffered D2O (prepared 5 mL with 4.5 mL of D2O and 0.5 mL of 10x protein dilution buffer and 0.2 μg/mL of peptide standard Glu-1-Fibrino peptide (CAS: 103213-49-6, Sigma)) to initiate deuteration at 22 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... were incubated 1 h at 37 °C in Duolink blocking buffer (Sigma-Aldrich) and then for 1 h at room temperature with a mix of the two primary antibodies directed against the target proteins (anti-biotin to detect new H3.3-SNAP-biotin and anti-gH2A.X to detect sites of DNA damage ...
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bioRxiv - Immunology 2023Quote: ... Organoids were stained overnight at 4°C in blocking buffer containing DAPI (Sigma), AlexaFluor-647-Phalloidin (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-protein complexes were eluted using Flag peptide (Millipore, Cat # F3290) at 150 µg/ml by incubating the beads at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: Proteins/peptides were labelled with atto-488 NHS ester (Sigma, 41698). Free dye was separated from labelled protein using G25 desalting columns ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were eluted with an excess of FLAG peptide (Sigma) or HA peptide (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were eluted with 50μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x Tris-buffered saline ...
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bioRxiv - Genetics 2019Quote: ... Bound proteins were eluted using a 3XFLAG peptide (Sigma-Aldrich, F4799) as described in [34] ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were eluted using 200 μg/mL 3xFlag peptide (F4799, Sigma) in lysis buffer by incubation on an orbital shaker (1400 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... immunoprecipitated proteins were eluted using 3X-FLAG peptide (Sigma - catalog # F4799) for 2 hours at 4°C according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...