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Citations for Millipore Sigma :
1901 - 1950 of 4665 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... followed by derivatization with 20 µl of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-butyldimethylchlorosilane (Sigma-Aldrich 375934) for 30 min at 60°C ...
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bioRxiv - Biochemistry 2020Quote: ... The peptide concentration was 20 μM in 100 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) (Sigma Aldrich) buffer (pH 7.4) ...
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bioRxiv - Developmental Biology 2020Quote: Genomic DNA extractions were performed with Extract-N-Amp or REDExtract-N-Amp™ Tissue PCR Kits (Sigma-Aldrich). Whole embryos or adult zebrafish fin-clips were lysed by incubation in a mixture of 50 μL extraction solution (Extraction Solution E7526 ...
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bioRxiv - Plant Biology 2020Quote: ... then silyated for 90 min at 60°C after adding 30 μl N-Methyl-N-(trimethylsilyl) trifluoroacetamide (Sigma-Aldrich). Incubations were carried out in a Vortemp 56 orbital shaker (Labnet ...
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bioRxiv - Plant Biology 2019Quote: ... then the samples were derivatized with an equal volume of N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide + 0.1 % tert-butyldimethylchlorosilane (MTBSTFA + 0.01 % t-BDMCS, Sigma-Aldrich) and incubated for 60 minutes at 60°C ...
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bioRxiv - Genetics 2021Quote: ... The whole embryo was then stained with a 0.025 N to 0.1 N Lugol’s iodine (I2KI) solution (Sigma-Aldrich, Germany) for 24 h and mounted in agarose for diceCT ...
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bioRxiv - Plant Biology 2021Quote: ... N-hexanoyl-L-homoserine lactone (C6-HSL) and N-3-oxotetradecanoyl-L-homoserine lactone (oxo-C14-HSL) (Sigma-Aldrich) at concentrations as indicated were used in this study.
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bioRxiv - Neuroscience 2022Quote: Lesion animals (n=18) received bilateral excitotoxic HPCv lesions via intracranial injections of N-methyl-d-aspartate (NMDA, Sigma, St-Louis ...
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bioRxiv - Biochemistry 2022Quote: ... followed by another incubation with 20 µL N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide with 1% tert-Butyldimethylchlorosilane (TBDMS) (Sigma) at 60 °C for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... they were incubated with 4 mg/mL of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma-Aldrich, Schnelldorf, Germany) dissolved in 100 mM 2-(N-morpholino ...
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bioRxiv - Physiology 2023Quote: ... followed by derivatization with 20 µl of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-butyldimethylchlorosilane (Millipore Sigma 375934) for 30 min at 60°C ...
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bioRxiv - Physiology 2024Quote: ... and silanized at 300°C in an atmosphere of N,N-dimethyltrimethyl silylamine (Sigma Aldrich, St. Louis, MO, USA). From here ...
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bioRxiv - Plant Biology 2023Quote: ... (2) addition of 15 μL of derivatization grade N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA; Sigma-Aldrich; Catalog Number 394866) and incubation at 37°C for 30 min with agitation at 750 RPM ...
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bioRxiv - Bioengineering 2024Quote: ... 2- (dodecylthiocarbonothioylthio)-2-methylpropionic acid (DDMAT, 98%), 2,2’-azobis(2- methylpropionitrile) (AIBN, 98%) and N, N-dimethylformamide (DMF, ≥99.8%) are purchased from Sigma Aldrich and used as received.
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bioRxiv - Bioengineering 2021Quote: ... Binding of biotinylated S1-RBD to coated VNAR-hFc was detected by streptavidin-HRP (Merck Millipore, 18-152). The signal intensities were collected for each primary VNAR-hFc antibody without competitor and compared to the signal when a competitor antibody was present ...
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bioRxiv - Synthetic Biology 2020Quote: ... Concentrations were determined by use of a standard curve (Supplementary Figure S1) generated by comparison to styrene (Sigma) standards mixed in dodecane with mock cell-free reactions containing green fluorescent protein in place of the biosynthetic enzymes that were incubated for 24 hours (Dudley et al. ...
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bioRxiv - Microbiology 2021Quote: ... Compounds were identified by comparing sample retention times with 16 available authentic standards (Figure S1) (Sigma – Aldrich, EUA). The concentrations of the identified compounds have not been determined.
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bioRxiv - Cell Biology 2024Quote: ... Target proteins were probed using their respective antibodies (Table S1) in 5% Bovine Serum Albumin (BSA, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2-infected cells were lysed in radio-immunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with 1% sodium dodecyl sulfate ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Immunology 2021Quote: LPS from Escherichia coli serotype 055:B5 (Sigma-Aldrich, L2880) was used ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were then plated in LB agar (Sigma-Aldrich) with ampicillin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... and subsequently transformed into Escherichia coli Rosetta (DE3) cells (Novagen). MBP-fused DBDs of BEH3 and BEH4 were expressed and purified in the same way as BES1 and BZR1.
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bioRxiv - Neuroscience 2021Quote: ... Lipopolysaccharides from Escherichia coli O55:B5 (LPS, L2880, Sigma-Aldrich) were dissolved in PBS to a concentration of 1 mg/mL ...
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bioRxiv - Neuroscience 2019Quote: We used lipopolysaccharides from Escherichia coli 0111: B4 (Sigma-Aldrich) dissolved in a saline sterile solution (0.9% sodium chloride ...
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bioRxiv - Physiology 2019Quote: ... animals were challenged with LPS (Escherichia coli 0111:B4, Sigma; 8 mg/kg ...
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bioRxiv - Microbiology 2019Quote: ... coli Rosetta gamiB (DE3) or Rosetta gami2 (DE3) cells (Novagen). Expression of Strep-KaiC7942 was carried out in E ...
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bioRxiv - Immunology 2019Quote: ... LPS (Escherichia coli serotype O55:B4) was from Sigma-Aldrich.
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bioRxiv - Biochemistry 2019Quote: ... coli strain Rosetta 2 (DE3) pLysS (EMD Millipore, Billerica MA). Cultures were grown at 37°C to A600=0.5 ...
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bioRxiv - Biophysics 2019Quote: ... coli K12 (MG1655) cells were grown in 2YT medium (Sigma, Tryptone 16.0 g/L ...
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bioRxiv - Biochemistry 2019Quote: ... coli lysate by Ni2+-affinity chromatography using HisSelect resin (Sigma), followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Microbiology 2021Quote: ... coli polar lipid extract (Avanti) was resuspended in chloroform (Sigma). The lipid solution was then dried off under N2 gas and resuspended in 10 mL of buffer B (50 mM Tris-HCl pH 7 ...
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bioRxiv - Neuroscience 2021Quote: ... coli cells Rosetta 2 (DE3) Competent Cells (Novagen, Sigma-Aldrich). Arc S67A and T278A DNA was amplified from point-mutant pCMV6-Arc-Myc-FLAG plasmids prepared using site-directed mutagenesis and subcloned similarly ...
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bioRxiv - Neuroscience 2021Quote: ... coli cells Rosetta 2 (DE3) Competent Cells (Novagen, Sigma-Aldrich). Arc S67A and T278A DNA was amplified from point-mutant pCMV6-Arc-Myc-FLAG plasmids prepared using site-directed mutagenesis and subcloned similarly ...
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bioRxiv - Biophysics 2021Quote: SSB protein from Escherichia coli was purchased from Sigma-Aldrich/Merck ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coli (LPS) and other chemicals were obtained from Sigma-Aldrich Chemical Co ...
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bioRxiv - Biochemistry 2020Quote: ... coli expression and thus required Rosetta2 DE3 pLysS cells (Novagen) for expression.
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bioRxiv - Microbiology 2021Quote: ... coli JM109 cells transformed with a pETBlue-2 plasmid (Novagen) containing the gene for alcohol dehydrogenase (ADH ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strains were routinely cultured in LB broth Miller (Sigma) (37 °C ...
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bioRxiv - Microbiology 2021Quote: ... 500ng/ml lipopolysaccharide (LPS) from Escherichia coli O55:B5 (Sigma) and 20ng/ml recombinant murine interferon-g (Peprotech ...
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bioRxiv - Biophysics 2020Quote: ... coli (mc4100) were grown on Luria Broth agar (Sigma-Aldrich) plates ...
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bioRxiv - Biochemistry 2022Quote: ... coli tRNA was either bought in bulk from Sigma-Aldrich or purified from a HB101 E ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli were cultured in Luria-Bertani (LB) media (Sigma-Aldrich) supplemented with 50 μg ml-1 kanamycin ...
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bioRxiv - Immunology 2022Quote: ... coli O55:B5 (LPS, Sigma-Aldrich cat: L2880 lot: 113M4068V) were REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Lineage Cocktail-PacBlue BioLegend Cat# 133310 ...
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bioRxiv - Microbiology 2022Quote: ... coli strains were grown in LB (Sigma, St Louis, MO) and streaked on LB plates having 15g/L agar when applicable ...
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bioRxiv - Microbiology 2020Quote: ... coli B834 (39, 40) containing plasmid pRARE2 (Novagen, Madison, WI) and transformants grown in ZYM-5052 Autoinduction Medium (41 ...
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bioRxiv - Systems Biology 2022Quote: ... coli LPS (1 and 100 ng/mL - Sigma; TLR4 ligand) and Pam3Cys ...
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bioRxiv - Immunology 2022Quote: ... coli O55:B5 (LPS, Sigma-Aldrich cat: L2880 lot: 113M4068V) were administered i.p ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli 0111:B4 (50 µg/mL final concentration, Sigma LPS25), Poly(I:C)(1 mg/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli 0111:B4 (1 µg/mL final concentration, Sigma LPS25) for indicated time ...