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Citations for Millipore Sigma :
1901 - 1950 of 2451 citations for Rpb1 CTD Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore) coated with AN18 IFNγ (1 µg/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A 20 μL sample of the supernatant and 1 μL of internal peptide standard (H2016, Sigma-Aldrich, Oakville, ON) were added to a 2 mL screw threaded HPLC vial (Chromatographic Specialties ...
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bioRxiv - Plant Biology 2023Quote: ... The washed beads were resuspended in 300 μL wash buffer containing 300 ng/μL of 3xFLAG peptide (F4799, Sigma) and 20 U/mL RNAsin (Promega ...
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bioRxiv - Immunology 2022Quote: ... and 175,000 were co-cultured with 25,000 OTII T cells with 200 ng/mL OVA323-339 peptide (Sigma-Aldrich) and varying ratios of Tregs ...
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bioRxiv - Neuroscience 2022Quote: ... was used in a concentration of 20 µM and autocamtide-2 related inhibitory peptide II (AIP-II; Merck Millipore) at 4 µM.
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bioRxiv - Developmental Biology 2022Quote: ... and peptide peak area ratios were generated against a spiked internal standard with bovine insulin (Sigma – 11070-73-8).
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bioRxiv - Plant Biology 2023Quote: ... TF complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore F4799). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843 ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... dried peptides were resuspended in 200 mM ammonium formate adjusted to pH 10 with ammonium hydroxide solution (Sigma-Aldrich). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The pulldowns were eluted from the FLAG and Strep resins using 0.1 mg/mL 3xFLAG peptide (GlpBio) or 2.5 mM desthiobiotin (Sigma-Aldrich), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged MSL proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Elution buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The resin was then washed with 3x10 bed volumes of lysis buffer and eluted with 800 µL (for 60 ml of cell suspension) lysis buffer (without protease inhibitor) supplemented with 0.2 mg/mL 3x Flag peptides (Sigma). The N-terminal 3xFlag tag was removed by incubation with the GST tagged Prescission Protease (GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... and 100 µM Na3VO4) before the protein was eluted with FLAG wash buffer containing 0.5 mg/mL 3xFLAG peptide (Sigma). LIMK1 concentration was determined by running alongside BSA standards on SDS-PAGE with Coomassie staining.
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bioRxiv - Molecular Biology 2023Quote: The FLAG-tagged peptide and Pgk1 were detected using the anti-FLAG M2 (Sigma; cat# F1365; 1:1000 dilution) and anti-Pgk1 22C5D8 (abcam ...
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bioRxiv - Biochemistry 2023Quote: ... with molar ratio of 1:5 (Nucleosome:SUV420H1) or 1:100 (Nucleosome: SUV420H1 C-terminal peptide) in the presence of 40 µM of SAM (Sigma). Samples were incubated on ice for 20 mins ...
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bioRxiv - Biophysics 2023Quote: ... The beads were washed 3 × 1 mL with membrane resuspension buffer for KRas before the beads were eluted with membrane resuspension buffer supplemented with 0.2 mg/mL 3X FLAG peptide (SAE0194, Sigma). Aliquots of samples were collected at each stage (input to anti-FLAG resin ...
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bioRxiv - Cell Biology 2023Quote: ... and FLAG-Sox4 protein was eluted by 5 rounds of competitive elution with 1× column volume (0.6 ml) of 100 μg/ml FLAG peptide (Sigma) in TBS ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed three times with buffer E and then mixed with 60 μg of 3xFLAG peptide (SIGMA) in 60 μl of buffer E for 1 h with shaking at 1400 rpm at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Molecular Biology 2023Quote: ... Protein was eluted with 4 CVs of imidazole-free wash buffer supplemented with 150 µg/mL 3xFLAG peptide (Sigma). This was analysed by SDS-PAGE before being concentrated ...
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bioRxiv - Biochemistry 2023Quote: ... The target protein was eluted twice by incubating the matrix with 0.5 mL of K buffer supplemented with 200 μg/mL FLAG peptide (Sigma) for 0.5 h ...
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bioRxiv - Biochemistry 2023Quote: ... dried peptides were dissolved in 20 μl of 2% formic acid supplemented with 20 mM citric acid (Sigma-Aldrich). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein complex was eluted with 500 μL elution buffer (0.4 mg/ml 3X Flag peptide (Millipore Sigma F4799) in wash buffer ...
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bioRxiv - Microbiology 2024Quote: ... The antigen-bound magnetic beads were washed five times with lysis buffer and eluted with 2.5 volumes of 3× FLAG peptide (150 ng/μl, Sigma) for 3 h at 4°C with gently shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... and the FANCI-FANCD2 complex was eluted with FLAG wash buffer containing 100 µg/mL 3X FLAG peptide (Sigma) and 5% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... was then added to cells for 20 minutes and subsequently replaced with an anti-FLAG antibody (1:1000 in blocking solution) (Cat #F3165, Sigma-Aldrich) for one hour ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 1 hour blocking in 3% BSA and overnight antibody incubation in Tris-buffered saline with 0.1% Tween-20 (Sigma-Aldrich, P1379) at 4 degrees ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies at appropriate dilutions (Supplementary Table 3) were prepared in blocking buffer with 0.1% Tween 20 (Sigma, 9005-64-5). The membrane was incubated in the primary antibody solution overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were first washed with 1x PBST (1x PBS with 20% Tween20) and then in blocking serum (5% serum (Cat. No. D9663, Sigma-Aldrich), 1% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... we started immunostaining by incubating the zebrafish samples in blocking solution (0.3% Triton X-100, 4% BSA and 0.02% sodium azide from Sigma-Aldrich in PBS) at room temperature for one hour ...
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bioRxiv - Developmental Biology 2020Quote: ... immunoblots were incubated overnight at 4⍰°C with primary antibodies diluted in Intercept blocking solution (Licor) against alpha-tubulin at 1:3500 dilution (Sigma, T6074), profilin-2 at 1:700 dilution (BosterBio ...
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bioRxiv - Plant Biology 2022Quote: ... the membranes were incubated with IRDye secondary antibody in Intercept® (PBS) Blocking Buffer with addition of 0.02% SDS and 0.2% Tween® 20 (Sigma, P1379) for an hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated again in blocking solution with primary antibody against choline acetyltransferase (ChAT; Millipore, AB144P, goat anti-ChAT, 1:1000) for 5 nights at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody reaction was carried out for 10 days at 4°C in blocking solution (1:1000 mouse monoclonal Tyrosine Hydroxylase antibody; T2928, Sigma-Aldrich). Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... After permeabilization with 0.5% Triton X-100 in PBS for 10 min at 4°C and blocking with 3% bovine serum albumin (BSA) (Wako) in PBS with 1% DAPI (Sigma-Aldrich) at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted in blocking solution and added to samples for 1 hour at room temperature (RT): ACTN1 (Sigma, A7811), TAGLN (Abcam ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Genomics 2020Quote: ... Subsequently sections were incubated for 1h at RT in PBS blocking buffer containing 5% horse serum (Sigma Aldrich, Cat. N: H0146), 0.3% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Physiology 2019Quote: ... Sections were incubated in blocking solution (1%BSA: Sigma Aldrich, 10% normal horse serum: ThermoFisher Scientific, UK 0.3M glycine: Sigma Aldrich, Dorset UK ...
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bioRxiv - Cell Biology 2019Quote: ... Following incubation with the appropriate fluorophore-labeled secondary antibodies in blocking solution for 40 minutes in the dark and counter-staining with DAPI (Cat.# D9542-5MG, Sigma-Aldrich) with intervening washes with 0.2% Tween in PBS (PBST) ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue sections were washed in PBS and subsequently blocked overnight at 4°C in blocking buffer (PBS containing 1% Triton X-100 (Sigma-Aldrich) and 5% goat serum (Gibco)) ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were rinsed 6 times in 1x PBS before being incubated with fluorescence-labeled secondary antibody (diluted in blocking buffer at 1:500 dilution ratio with 1/10000 Hoechst 33258, Sigma #94403) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation with the primary antibodies diluted in a 1:1 solution of blocking buffer to PBS with 0.2% Tween (PBST) (Sigma-Aldrich, P7949) overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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Epicardial slices: a 3D organotypic model for the study of epicardial activation and differentiationbioRxiv - Bioengineering 2020Quote: ... followed by permeabilization for nuclear antigens (0.1% Triton X-100 in PBS for 30 min) and blocking for 1 hour at room temperature with 20% Goat serum (Sigma-Aldrich) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), and rabbit anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... in phosphate buffered saline (PBS) at room temperature for 10 mins and treated with blocking solution (1.5% bovine serum albumin (Sigma-Aldrich, #A3059) / 0.1% fish skin gelatin / 0.1% Triton X-100 in PBS/NH4Cl ...
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bioRxiv - Neuroscience 2021Quote: ... They were incubated with the following primary antibodies in blocking solution at 4°C overnight: mouse monoclonal anti-GFAP (1:1000, G3893; Sigma-Aldrich), rabbit polyclonal anti-GFAP (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were first incubated for 1 h at room temperature in a blocking solution containing 4 % bovine serum albumin (Sigma-Aldrich) in PBS-T ...
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bioRxiv - Cancer Biology 2019Quote: ... The samples were incubated overnight at 4 °C with the indicated primary antibody prepared in blocking/permeabilization solution (with 0.1% Triton X-100): rabbit polyclonal antibody against GLUT-1 (1:250; Millipore, 07-1401), rabbit monoclonal antibody against HK-1 (1:200 ...