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Citations for Millipore Sigma :
1751 - 1800 of 2451 citations for Rpb1 CTD Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Then slices were washed with 0.2% Tween-20 and incubated with a blocking buffer consisting of 5% NGS (Sigma-Aldrich) and monoclonal mouse anti-ankyrinG antibody (1:500 ...
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bioRxiv - Immunology 2019Quote: ... The plates were washed three times with PBS (pH 7.4) and incubated with blocking buffer (2% bovine serum albumin (Sigma-Aldrich) in PBS-Tween 20 (0.05% ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then treated with 5% BSA blocking solution for 1 h and the incubated with mouse monoclonal anti-HA (Sigma) antibody and rabbit anti-Flag antibody (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... and non-specific binding sites were blocked by immersing the membranes in blocking buffer containing 5% non-fat milk (70166, Sigma) in TBST buffer for 1 h on a shaker at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... For ZO-1 images cultures were fixed in 4% PFA for 20 min at room temperature, followed by permeabilization (Permeabilization Buffer set, Ebioscience) and blocking with 2% BSA solution (Sigma). Cells were stained with anti-ZO-1 mouse mAb (Thermo-Fischer ...
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bioRxiv - Genomics 2021Quote: ... Coverslips were washed with blocking buffer (4X-SSC/0.1% Triton X-100/4% BSA (Bovine serum albumin; Sigma, catalog #A7906)) at RT for 30 mins ...
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bioRxiv - Bioengineering 2021Quote: ... at −20°C for 6 minutes followed by a 1-hour incubation in blocking buffer containing 10 wt% BSA (Sigma) in PBS as previously described7 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... transferred across three drops of filtered PBS and then incubated on drops of filtered blocking solution containing 2% BSA (Sigma) in PBS for 10 min ...
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bioRxiv - Physiology 2021Quote: ... Muscles were then incubated in blocking solution containing rabbit anti-heavy NF200 primary antibody (N4142 Sigma, Germany; 1:200 dilution) and anti-synaptophysin antibody (1:25 dilution as above) ...
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bioRxiv - Microbiology 2021Quote: Single-nucleotide EdU-labelled slides were incubated in blocking solution (1% BSA in PBS) for 30 mins and then 1:100 anti-centrin antibody (clone 20H5, Millipore) in blocking solution for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532, rabbit anti-Gluc from Nanolight 401P, mouse anti-GAD67 from Millipore MAB5406 ...
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bioRxiv - Biochemistry 2021Quote: ... After blocking the membrane with 2 % non-fat dry milk dissolved in PBS + 0.1 % (v/v) tween-20 (PBST; Sigma-Aldrich), membranes were incubated with primary antibodies for either 2 hours at room temperature or overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... The sections were then incubated in the blocking solution (PBS containing 10% donkey serum (cat no: S30-100ml, Millipore Sigma), 2% BSA (cat no ...
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bioRxiv - Neuroscience 2021Quote: ... Inner ear was firstly immersed in blocking solution (4% BSA (V900933, VETEC) in 0.5% PBS-Triton X-100 (T8787, Sigma-Aldrich)) for 1 day ...
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bioRxiv - Neuroscience 2021Quote: ... and the non-specific binding was blocked using a blocking buffer containing 3% bovine serum albumin (BSA) (Sigma-Aldrich, #A9647) in PBST (137 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were then washed 3 x 5 min with 3% BSA in PBS-T and incubated with the following DNA dye and secondary antibody dilutions in blocking solution for 3 hours at room temperature: Hoechst 33342 (0.2 mM, Sigma-Aldrich), Goat Anti-Chicken Alexa Fluor® 647 (4 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Neuroscience 2020Quote: ... They were blocked by incubating with Duolink blocking solution according to the manufacturer’s indications (Duolink® in situ PLA kit, Sigma). Cells were incubated with rabbit polyclonal anti-HA.11 and mouse monoclonal IgG1k anti-GFP antibodies at 4°C overnight ...
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bioRxiv - Genomics 2021Quote: ... then permeabilized and blocked for non-specific binding in blocking buffer containing 3% normal horse serum and 0.25% Triton X-100 (Sigma X100) in a humidified chamber for 1 hr at room temperature8 ...
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bioRxiv - Genomics 2021Quote: Sections were washed in 0.1M TBS and then non-specific binding site blocking and cell permeabilization was performed with blocking buffer containing 3% normal horse serum and 0.25% Triton X-100 (Sigma X100). Sections were incubated with primary antibody (see list below ...
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bioRxiv - Neuroscience 2022Quote: ... permeabilization and blocking of non-specific binding with 0.2 % Triton-X and 3 % bovine serum albumin (BSA, A7906, Sigma Aldrich), samples were incubated overnight at 4 °C with a solution of PBS containing 0.2 % Triton-X ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with PBS three times and then incubated in blocking solution (PBS supplemented with 0.1% Triton-X-100 [X100, Sigma-Aldrich] and 10% normal donkey serum [017-000-121 ...
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bioRxiv - Cancer Biology 2022Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in blocking buffer containing 0.2% Tween-20: Puromycin (1:10000, Millipore #MABE343), APOE (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Blocking of unspecific antigens was carried out by incubation in 3% bovine serum albumin (BSA; Sigma-Aldrich; dissolved in PBST) for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated (30 min at room temperature) in blocking buffer with (permeabilized cells) or without (non-permeabilized cells) 0.05% (w:v) saponin (Sigma-Aldrich, 558255), 0.5% (w:v ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: The nitrocellulose membrane was imaged for total protein and then blocked for one hour in blocking buffer (5% w/v bovine serum albumin [Sigma, SKU A7906] in TBS-T buffer [50mM Tris-HCl ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 0.1 % Tween-20 and 2 mM EDTA before incubating in a blocking solution (3 % acetylated bovine serum albumin (Sigma, MO), 0.1 % Tween-20 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 3 times in PBS (10 minutes per wash) and then incubated for 1 hour in blocking solution containing 3% normal donkey serum (NDS, SC30-100ML, Merk Millipore) and 0.2% Triton X-100 (648464 ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated overnight in primary antibodies targeting LC3 (1:2000) and p62 (1:2000) and aSyn (1:500) diluted in blocking solution with 0.1% Tween-20 (Sigma-Aldrich). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific binding sites were blocked by incubation of the tissue in blocking solution (5% normal goat serum (Sigma, G6767), 1% blocking reagent (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex antibody cocktails were made in a whole-mount blocking solution consisting of 5% heat activated normal donkey serum (Sigma Millipore), 20% Dimethyl sulfoxide (Sigma Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... Washed cells were first incubated for 1 h at room temperature (RT) with blocking solution (3% bovine serum albumin [BSA, Sigma, A7906] and 0.5% Triton X-100 [Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... and exposed to blocking solution containing 2% bovine serum albumin or 5% normal goat serum (all from Sigma Aldrich, USA). The fixed cells were incubated overnight at 4 °C with the following primary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were blocked for 1 hour with blocking buffer (Cyto-TBS + 2% bovine serum albumin (BSA, Sigma Aldrich, 1002695029)) and incubated with a primary antibody overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse ...
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bioRxiv - Microbiology 2023Quote: ... tissues were treated with a blocking buffer [PBT containing 1% bovine serum albumin (BSA, heat shock fraction; Sigma-Aldrich, A7906)] for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Samples were permeabilized in 0.1% Triton-X-100 for 3 min and then incubated in a blocking solution consisting of 10% FBS (Sigma-Aldrich), 0.01% sodium azide (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... cryo-sections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were washed in a phosphate buffer saline followed by blocking of the non-specific binding sites with 5% Chemiblocker (Millipore), and 0.2% Triton in phosphate buffer saline ...
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bioRxiv - Bioengineering 2022Quote: ... To improve antibody specificity cells were incubated for 30 min at room temperature in a blocking solution consisting of 0.03% v/v Triton X-100 (Sigma, T8787) and 3% v/v bovine serum albumin (Sigma ...
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bioRxiv - Physiology 2023Quote: ... Samples were blocked for three hours at room temperature in blocking solution [5% normal goat serum (MP Biochemicals) plus 5% bovine serum albumin (Sigma) in PBST] and then incubated overnight at 4°C in primary antibodies diluted in blocking solution ...
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bioRxiv - Molecular Biology 2022Quote: ... were washed three times in blocking buffer (0.5% BSA in 1xPBS) and incubated overnight at 4°C with 20μL antibody (anti-FLAG; Sigma F1804-1MG). 30×10^6 of ESCs crosslinked with formaldehyde as described above were resuspended in 500μL RIPA Buffer (50mM Tris-HCl ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight at 4°C with primary antibodies in a 1:1 solution of TBST (TBS + 0.05% Tween20) and LiCor blocking buffer (Rabbit anti-GFP, Torrey Pines Scientific TP401; and Mouse anti-alpha-tubulin, Sigma T5168). Membranes were then washed 5x times with TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Permeabilisation was achieved by incubation for 1h in freshly prepared blocking solution (PBS with 5% (v/v) normal donkey serum (D9663, Sigma) and 0.3% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... All primary antibodies were incubated overnight with membranes in TBS blocking buffer supplemented with 0.1% Tween-20 (Sigma-Aldrich # P1379), while secondary antibodies (HRP conjugate ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS (3 x 10 mins) then incubated in a blocking solution comprised of PBS containing 0.5% Triton X-100 (Sigma-Aldrich, Catalogue #X100-500ML ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 7.5ml Intercept PBS blocking buffer with 0.1% Tween (SynGAP1; 1:1000dil, Invitrogen, PA1-046, Gapdh; 1:15000, Sigma-Aldrich G8795). Membranes were incubated with the primary antibody solution overnight at 4[ ...
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bioRxiv - Molecular Biology 2023Quote: ... in PBS for 5 minutes at room temperature and the non-specific binding of the antibodies was prevented by applying a blocking solution (PBS supplemented with 5% BSA (Sigma) or 5% goat serum (for Aurora B kinase immunostaining in heart sections ...
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bioRxiv - Molecular Biology 2023Quote: ... a DNA hydrolysis step between the permeabilization and the blocking step was performed by the addition of 2M HCL (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS for 30 min and exposed to blocking buffer (2 wt% FBS, 0.5 wt% bovine serum albumin (Sigma Aldrich) and 0.1 wt% Tween-20 (Sigma Aldrich ...