Labshake search
Citations for Millipore Sigma :
1851 - 1900 of 2451 citations for Rpb1 CTD Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
-
bioRxiv - Immunology 2019Quote: ... Murine BMDCs at day 8 of cultivation were incubated with 3-fold higher numbers of CD4+ CD25− T-cells (OT-II or OT-II.RAG-1-/-) and 200 ng/ml OVA peptide (323-339, grade V, Sigma) supplemented or not with our different stimuli ...
-
bioRxiv - Plant Biology 2019Quote: ... 150 mM NaCl and 10 mM HEPES pH 7.4 and eluted with 100 μg ml−1 3×FLAG peptide (Sigma).
-
bioRxiv - Bioengineering 2019Quote: ... The gelatin and peptide solutions were prepared by dissolving in deionized water (supplied by a Millipore Milli-Q system) at 10 wt % by heating at 50°C for 30 min ...
-
bioRxiv - Biophysics 2019Quote: ... The BBSome:ARL6 complex was eluted from the column with a total of 10 ml of 0.1 mg/ml Flag peptide (Sigma). Elution was performed in 5 steps ...
-
bioRxiv - Cell Biology 2020Quote: ... and again with wash buffer before elution buffer (wash buffer with 300 ng 3x-FLAG peptide (Sigma Aldrich, F4799)) was added and incubated with beads overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... 50 mM HEPES pH 7.4, 200 mM NaCl, 2 mM MgAc2, 0.25 mg/mL 3X FLAG peptide -Sigma F4799) with gentle end-over-end mixing ...
-
bioRxiv - Immunology 2020Quote: 1×107 draining lymph node or spleen cells were restimulated for 6 hours with 10μM MCC peptide with the addition of 5μM Brefeldin A (Sigma Aldrich) for the final 4 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... This was followed by a peptide clean-up as per the zip-tip protocol (Millipore Sigma; Cat# C18 ZTC18S008). 20ul of 0.1% formic acid (in acetonitrile ...
-
bioRxiv - Plant Biology 2021Quote: ... Polysomes were eluted by resuspension of the washed gel in polysome wash buffer containing 3×FLAG peptide (Sigma-Aldrich). Total RNA was extracted from the final elution using Direct-zol RNA kit (Zymo research) ...
-
bioRxiv - Plant Biology 2021Quote: ... The samples were prepared in 1:1 mixture of H2O-ACN with 0.1% TFA by mixing an equal amount of peptide fractions (0.5 μl) with α-cyano-4-hydroxycinnamic acid (CCA)/2,5-dihydroxy benzoic acid (DHB) matrix (Sigma) and spotted on a stainless-steel plate and air-dried ...
-
bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted in a final volume of 600 μL and filtered with 0.22 μm PVDF filters (Merck Millipore) before dried in a centrifugal vacuum concentrator ...
-
bioRxiv - Microbiology 2019Quote: ... The antigen bound Magnetic Beads were washed five times with the lysis buffer and eluted by 2.5 volume of 3×FLAG peptide (150 ng/µl, Sigma) for 3h at 4°C with gently shaking ...
-
bioRxiv - Cell Biology 2021Quote: ... and kinetochores particles were eluted by gentle agitation of beads in elution buffer (Buffer H + 0.5mg/mL 3FLAG Peptide (Sigma)) for 30 min at room temperature.
-
bioRxiv - Biophysics 2020Quote: ... The protein was eluted from the column with Wash Buffer supplemented with 3 mg mL-1 1D4 peptide and concentrated to 4.3 mg mL-1 using a 100-kDa concentrator (Millipore) before further purifying by size exclusion chromatography using a Superose 6 increase 10/300 GL column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2022Quote: ... Peptides were acidified with 1% trifluoroacetic acid (TFA) and then desalted using C18 ZipTip® (Millipore cat # ZTC18 5096). Dried peptides were resuspended in 10 µL of 0.1% TFA in water ...
-
bioRxiv - Cancer Biology 2022Quote: ... The proteins were eluted by incubating the beads with 35 μl 100 µg/ml 3xFLAG-Peptide (F4799, Sigma Aldrich) at room temperature for 20 min or by adding Laemmli Sample Buffer (1610747 ...
-
bioRxiv - Immunology 2020Quote: ... Plasmids encoding the selected peptides were isolated in 96-well format using the Montage Plasmid MiniprepHTS Kit (MilliPore, LSKP09604) on a MultiscreenHTS Vacuum Manifold (MilliPore ...
-
bioRxiv - Cell Biology 2021Quote: ... then resuspended in 100 μL of buffer (50 mM Tris, 150 mM NaCl, 1mM EDTA, pH 7.4) with Flag peptide (Sigma) and rocked overnight at 4°C overnight ...
-
bioRxiv - Microbiology 2020Quote: ... For elution of FLAG-tagged proteins and their complexes 100-300 µL of 0.1 mg/mL FLAG3 peptide (Sigma) was added to samples ...
-
bioRxiv - Microbiology 2020Quote: ... cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18) ...
-
bioRxiv - Physiology 2022Quote: ... 150 μl of 0.75mg/ml myelin oligodendrocyte glycoprotein 33 to 55 peptides (MOG35–55) mixed with Complete Freund’s Adjuvant (FS8810; Sigma-Aldrich) was injected subcutaneously into the tail base of 7 to 11-week-old female C57Bl/6 mice ...
-
bioRxiv - Immunology 2022Quote: ... PBMC were restimulated with 1μg/mL peptide + anti-CD28 in the presence of 1μg/ml Brefeldin A (Sigma-Aldrich) for 16h at 37°C ...
-
bioRxiv - Bioengineering 2022Quote: ... (Ac)GCRE-ISAFLGIPFAEPPMGPRRFLPPEPKKP(Am) (BM-binder).39 All peptides were reconstituted in acidic (pH 5.5) Milli-Q water (Millipore). The concentration of free thiols in all peptides was determined using Ellman’s reagent (Sigma Aldrich).
-
bioRxiv - Pathology 2019Quote: ... For MALDI-TOF/TOF/MS analysis dried peptides were dissolved in peptide resuspension solution (0.1% TFA in 5% ACN) and desalted/concentrated using C18 zip tips (Merck Millipore) as per the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... The peptide concentration was 20 μM in 100 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) (Sigma Aldrich) buffer (pH 7.4) ...
-
bioRxiv - Molecular Biology 2019Quote: ... the remaining resin was washed with 20 mL of A buffer twice and was eluted with 5mL of A buffer containing 0.8 mg/mL 3X Flag peptide (Sigma). The peptide was incubated with the resin for 20 min before elution ...
-
bioRxiv - Cancer Biology 2019Quote: ... 0.1% trifluoroacetic acid (TFA) was used to extract peptides and concentrated by C18 zip-tips (Millipore,-C18, P10 size) on Xcise (Proteome Systems) ...
-
bioRxiv - Plant Biology 2019Quote: ... and bound protein was eluted with 60 μL of 0.5 mg/mL 3×FLAG peptide (Sigma-Aldrich, MO, USA) for 1 h at 4°C ...
-
bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
-
bioRxiv - Genetics 2020Quote: ... Beads were then washed three times with lysis buffer and eluted in lysis buffer supplemented with 0.5mg/mL 3xFLAG peptide (Sigma). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
-
bioRxiv - Molecular Biology 2020Quote: ... twice with TBS and eluted by incubating samples with Flag elution buffer (200 µg/ml Flag peptide (Sigma-Aldrich) in 1x TBS ...
-
bioRxiv - Cell Biology 2020Quote: ... the beads were washed with 1% Trition buffer three times and then incubated in 30 μl of 1% Trition buffer with 0.2 μg/μl of 3xFLAG peptide (Sigma) at room temperature for 20 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and eluted by incubation in TAP buffer supplemented with 0.2 mg/mL HA peptide (Sigma aldrich). Eluates were washed and concentrated by Vivacon 2 ...
-
bioRxiv - Immunology 2019Quote: EAE was induced by subcutaneous immunization with 200 μg MOG35–55 peptide (HZI, Braunschweig) emulsified in CFA (Sigma-Aldrich), followed by intravenous injection of 200 ng pertussis toxin (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... Yta7 and mutant versions were eluted in 1 CV of lysis buffer containing 150 mM KCl with 0.5 mg/ml 3x FLAG peptide (Sigma), followed by 2 CV of lysis buffer containing 150 mM KCl with 0.25 mg/ml 3x FLAG peptide ...
-
bioRxiv - Microbiology 2020Quote: ... the peptide corresponding to the C-terminus region of BtrS (CALREALRERGYDSVP) were conjugated with hemocyanin from keyhole limpets (Sigma) by using 3-maleimidobenzoic acid N-hydroxysuccinimide ester (Sigma) ...
-
bioRxiv - Biochemistry 2021Quote: ... 100 mM NaCl) and eluted with wash buffer containing 0.15 mg/ml 3x FLAG peptide (Sigma cat no. F4799) (Extended Data Fig 1b) ...
-
bioRxiv - Immunology 2021Quote: ... and two pools of S2 peptides (pools 3 and 4) (final concentration of 2μg/mL) on IPVH-membrane plates (Millipore) coated with 5μg/mL anti-mouse IFNγ (AN18) ...
-
bioRxiv - Biochemistry 2019Quote: ... followed by elution of carboxylase by nutation in 200 μl Buffer A with 100 μg/mL FLAG peptide (Sigma) for 1 h at 4 °C and centrifugation (10000 × g).
-
bioRxiv - Genetics 2019Quote: ... the column was inverted and bound proteins were eluted with 10 ml of buffer PT containing 0.1 mg/ml Flag peptide (Sigma). The eluate fractions were dialyzed against buffer A [25 mM Hepes-NaOH (pH 7.5) ...
-
bioRxiv - Biochemistry 2021Quote: ... Solvents and reagents used in peptide synthesis were sourced as follows: DMF and DCM (ACS grade) from Sigma-Aldrich; piperidine (99% ...
-
bioRxiv - Immunology 2020Quote: ... splenocytes were stimulated with S peptide pools at a final concentration of 2μg/ml on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (clone AN18 ...
-
bioRxiv - Biochemistry 2021Quote: ... sample was placed on a magnetic rack and the supernatant containing peptides was collected and an additional elution (50μl) was performed using 2% dimethyl sulfoxide (DMSO, Sigma) prior sonication in a water bath for 1 min ...
-
bioRxiv - Neuroscience 2021Quote: ... were labelled by calcitonin gene-related peptide (CGRP) immunoreactivity using a monoclonal anti-CGRP antibody (1:5000; Sigma #C7113) raised in mouse ...
-
bioRxiv - Immunology 2021Quote: ... peptide cocktails were combined with membrane disrupting antimicrobials effective against relevant pathogens beginning at: 10 μM Cecropin A (Sigma), 500μg/mL ampicillin ...
-
bioRxiv - Molecular Biology 2021Quote: ... Beads were washed three times with lysis buffer-PBS at 1:4 and eluted with 3xFLAG peptide (Sigma-Aldrich) or Laemmli sample buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNCs were eluted after 3 sequential 10 min incubations at room temperature in the RNC buffer that contained 0.2 mg/mL 3x FLAG peptide (Sigma). The elutions were combined and centrifuged at 186,000 x g at 4 °C for 1.5 h in a (TLA-120.1 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Beads were then washed three times with lysis buffer and eluted in lysis buffer supplemented with 0.5mg/mL 3xFLAG peptide (Sigma). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
-
bioRxiv - Cell Biology 2022Quote: ... The beads were washed with IP lysis buffer and incubated with 5 mg ml−1 3× FLAG peptide (Sigma–Aldrich ...