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Citations for Millipore Sigma :
2151 - 2200 of 4429 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... After Ad6 viruses had been added and the plates had been blocked with bovine serum albumin (Sigma), dilutions of the mouse sera were added ...
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bioRxiv - Microbiology 2024Quote: ... Binding was detected by incubating the plates sequentially with HRP-linked rabbit anti-human IgG (A8792, Sigma). After washing ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... which were freshly cultivated from long-term storage on Sabouraud 4% Dextrose Agar plates (SDA; Sigma-Aldrich) at 23°C and had been harvested in sterile Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... we used wells (96-well plate) coated with poly-L-lysine (Sigma-Aldrich, Life Science, Catalog #P4707) to improve cell adhesion ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 3 times with PBS-T (Sigma cat. # P3563-10PAK) and blocked for 1h with 1% BSA (for the mouse sera ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed four times and then incubated with 70 µl of Alkaline Phosphatase Solution (Sigma Aldrich) for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines were tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) using JumpStart Taq DNA Polymerase (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... Indexing and amplification was done using the KAPA HiFi HotStart PCR kit (Sigma-Aldrich, KK1508) with Index oligonucleotides (sequences were adapted from Illumina).
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative (q)PCR was performed using SYBR Green Jump Start Taq Ready Mix (Sigma S4438) and Intron-spanning primer pairs (Supplementary Table 5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA was purified using the High Pure PCR Product Purification Kit (11732676001 Sigma-Aldrich). Quantitative Real Time PCR reactions were performed with the TB Green™ Premix Ex Taq™ II (TAKARA ...
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bioRxiv - Molecular Biology 2021Quote: ... A cDNA library was generated using the cDNA Library Kit from Thermo Fisher Scientific (catalog A48571) and the Reverse transcription PCR kit from Millipore Sigma (Product No. HSRT100), with specific primers were designed for each target gene (Supplementary Table 3) ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR product were assembled into KpnI and EcoRI difgested pZDonor-AAVS1 Puromycin plasmid (Sigma-Aldrich) and ligated using the Quick Ligase Kit (New England Biolab) ...
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bioRxiv - Microbiology 2019Quote: ... Taq Polymerase and restriction enzymes for cloning and PCR methods were purchased from Sigma Aldrich. PCR cycle was as follows ...
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bioRxiv - Immunology 2019Quote: ... cDNAs were used as a template for PCR using the KOD high fidelity polymerase (Novagen) and a mixture of 8 VH family and IgM-specific primers (listed below ...
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bioRxiv - Genomics 2020Quote: ... Sequences were PCR amplified from zebrafish genomic DNA using the primers in SupplementaryTable16 (Sigma-Aldrich), with the proof-reading iMax ™ II DNA polymerase (INtRON Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... and regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma, MP0035), using JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was cloned at the NdeI and XhoI restriction sites of pET30a (Novagen) to add a C-terminal 6x-his tag using the Gibson assembly cloning system ...
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bioRxiv - Microbiology 2020Quote: ... The PCR fragment was subsequently cloned into the BamHI-HindIII restriction sites of pETDuet (Novagen) to generate the recombinant plasmid pETDuet-DHS1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The designed promoters were obtained by either PCR (KOD Hot-Start DNA polymerase, Merck-Millipore). PCR amplification was performed by KOD Hot Start DNA polymerase (Merck Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... The amplicons were purified using GFX PCR DNA and Gel Band Purification Kit (Sigma-Aldrich), according to the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines were tested for mycoplasma using Lookout Mycoplasma PCR kit (Sigma-Aldrich MP0035).
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bioRxiv - Genomics 2022Quote: ... PCR amplifications were set up in 25μl total volume reaction mixture containing water (Sigma-Aldrich) (18.4μl) ...
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bioRxiv - Cell Biology 2022Quote: ... Regular mycoplasma testing was performed using the LookOut Mycoplasma PCR detection (Sigma, Cat. No. MP0035).
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bioRxiv - Microbiology 2020Quote: ... The 3’UTR was determined by RT-PCR with an oligo-dT anchor primer (Sigma) as described58 with primers found in Table 4 ...
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bioRxiv - Microbiology 2020Quote: ... RT and PCR primers for the determination of the 3’UTR were obtained from Sigma. The remaining primers were purchased from TAG Copenhagen.
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bioRxiv - Developmental Biology 2020Quote: The four lncRNAs tested were PCR-amplified with KOD Xtreme™ DNA Hotstart Polymerase (Millipore) from their 5’ end up until the last codon of the sORF to be tested ...
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bioRxiv - Immunology 2020Quote: ... and cells were verified as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma).
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bioRxiv - Plant Biology 2021Quote: ... Positive plants were further confirmed by PCR and western blot using GFP antibodies (Sigma-Aldrich). The positive T2 generation transgenic plants were evaluated in a growth chamber.
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Comprehensive profiling of antibody responses to the human anellome using programmable phage displaybioRxiv - Immunology 2022Quote: ... 2 μL of sample was added to 1x PCR Master Mix (Sigma-Aldrich, Basel, Switzerland) and the 4 degenerate primers at a final concentration of 1 μM each in a final volume of 25 μL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplified fragments were purified using spin columns (GenElute TM PCR Clean-Up Kit, Sigma- Aldrich) and were checked on agarose gel electrophoresis ...
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bioRxiv - Cancer Biology 2022Quote: ... Clones were screened for N-Se loss by PCR using REDTaq ReadyMix (Sigma, R2523-100RXN) and custom primers (5’-AGCGAGACTCCGTCTAGAAA-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into the NdeI and XhoI sites of plasmid pET21b (Novagen). To generate a plasmid for the expression of a cleavable GST tagged GbpA ...
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bioRxiv - Genomics 2019Quote: ... We performed qRT-PCR using FastStart Universal SYBR Green Master Mix with ROX (Sigma, 4913914001) on a ViiA 7 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... using PCR primers Ad1_noMX and Ad2_Barcode (Sigma-Aldrich, see Table 2, (Buenrostro et al., 2013) for 5 initial cycles (1 cycle ...
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bioRxiv - Genomics 2019Quote: ... Tail snips were prepared using the REDExtract-N-Amp Tissue PCR Kit (Sigma-Aldrich XNAT) and were screened for Ube3a deletion using PCR primers spanning the expected deletion site ...
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bioRxiv - Genomics 2021Quote: ... Cells tested negative for mycoplasm contamination with the LookOut Mycoplasm PCR Detection Kit (Sigma; MP0035) and Jumpstart Taq DNA Polymerase (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested with SacI und SalI and cloned into pET-24a (Novagen), resulting in plasmid pET24a-OLA1-6xHIS ...
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bioRxiv - Microbiology 2019Quote: ... Digested PCR product was ligated into a modified T7 promotor-based pET33b expression vector (Novagen) that contained the super-folding variant of green fluorescent protein encoding gene with C-terminal 6xHis-tag and a modified multiple cloning site ...
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bioRxiv - Immunology 2021Quote: ... Positive clones were selected and screened by PCR using the Expand Long Range dNTPack (Sigma), PCR buffer supplemented with 2% DMSO and the primer pairs for the 5’ region (LR 5’ fw ...
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bioRxiv - Microbiology 2022Quote: ... and were verified to be mycoplasma free by a LookOut Mycoplasma PCR Detection kit (Sigma). C ...
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bioRxiv - Bioengineering 2022Quote: ... Fragments were amplified by PCR using Q5 polymerase and assembled into a pET28a(+) vector (Novagen) via Gibson Assembly using reagents from New England Biolabs (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... Captured products were amplified through PCR using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore), cloned into the pET23-P2-D12A-THR expression backbone ...
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bioRxiv - Genomics 2022Quote: ... Libraries were purified using the High Pure PCR Production Purification Kit (11732676001, Roche / Sigma-Aldrich). Libraries were validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR) ...
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bioRxiv - Immunology 2022Quote: ... cDNAs were mixed with indicated gene-specific primers and SYBR green PCR Master Mix (Sigma), and qRT-PCR was performed on an Applied Biosystems 7900HT Fast Real-Time PCR system.
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bioRxiv - Immunology 2022Quote: ... PCRs with 50 ng of prepared cDNAs were carried out using Jumpstart Taq polymerase (Sigma) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Mice were genotyped by PCR on tail genomic DNA using Red-N extract kit (Sigma), with primers indicated in Supplemental table S3.
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bioRxiv - Cell Biology 2023Quote: ... DNA purification was performed using a SpinPrep™ PCR Clean-up Kit (Merck Millipore, Germany). DNA fragments were assayed by qRT-PCR using the primer sequences listed below ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) using JumpStart Taq DNA Polymerase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA extraction was performed using Extract-N-Amp(tm) Tissue PCR Kits (Sigma/Aldrich). For cDNA synthesis ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloning the PCR product between the NdeI and BamHI sites of pET9a (EMD Millipore). PCR-amplified DNA sequences were confirmed by Sanger sequencing.