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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Their relative expression was monitored by ELISA using anti-FLAG-HRP (Sigma) or anti-HA-HRP antibodies (Roche Diagnostics) ...
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bioRxiv - Biochemistry 2021Quote: ... or alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for Elisa (Sigma P7998) were added as a substrate ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated overnight with anti-human IgG (Sigma Aldrich I5260) at 1:5 000 in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Chromatin immuno-precipitation was carried out using 2 µL of a commercially available anti-RNA polymerase II subunit B1 phospho-CTD Ser-5 antibody (Millipore, clone 3E8, cat. no. 04-1572) for RNAP II ...
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bioRxiv - Microbiology 2021Quote: ... moscoviensis was assessed by measuring total cell protein with the enhanced test tube protocol of the BCA protein assay (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Protein content was measured by the BSA (cells) or microBSA (EVs) protein assay according to the manufacturer’s instructions (Sigma Aldrich, USA).
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bioRxiv - Genomics 2021Quote: ... Cells were permeabilized by 0.1% Triton X-100 for 5min at RT and stained with antibody against the mitosis specific antigen pMPM2 (Millipore, 05-368, 0.2µl/10million cells) for 50min at RT ...
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bioRxiv - Microbiology 2022Quote: ... 2 µM FITC-TB_KKG6K was added along with 5 μM of the cell wall specific fluorescent dye calcofluor white (CFW; Sigma-Aldrich, St. Louis, MO, USA) to 800 μL of the C ...
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bioRxiv - Neuroscience 2019Quote: The level of RET phosphorylation in the cells was analyzed by Western blotting of immunoprecipitated RET with phospho-tyrosine-specific antibody (1:1500 Merck Millipore Cat# 05-321, RRID:AB_309678) as described previously (Leppanen et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were seeded in culture dishes and the specific pharmacological inhibitors radicicol (inhibitor of ATP-binding site of Hsp90, Sigma-Aldrich, Merck, Darmstadt, Germany), cyclosporine A (inhibitor of Cyp activity ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed with 80% cold acetone and incubated with murine monoclonal anti-influenza A or B nucleoprotein (Millipore Billerica, MA, USA) followed by peroxidase-conjugated goat anti-mouse IgG (Kirkegaard and Perry Laboratories ...
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bioRxiv - Microbiology 2019Quote: ... DT40 cells (immortalised chicken B cell line [16]) were maintained in Roswell Park Memorial Institute (RPMI) media supplemented with L-glutamine (Sigma-Aldrich, Merck), sodium bicarbonate (Sigma-Aldrich ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... HEK293T cells were seeded in a 6-well plate so that they were 70-90% confluent the next day (∼0.8x10^6 - 1x10^6 cells/well in 2 mL of standard growth media containing 10% FBS (Millipore, TMS-013-B) and 1x Penicillin-Streptomycin (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were maintained at a density of 0.2-1.5×105 cells / cm2 by passaging using TrypLE (12563011) every 24-48h on 0.1% gelatin-coated dishes (Millipore cat ES-006-B) at 37°C and 7% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... TMB was removed and cells were washed twice with 1XTBS followed by incubation with 0.2% Janus green B (Sigma, Cat. no. 201677) (w/v ...
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bioRxiv - Systems Biology 2019Quote: ... was grown with 1 million seeded cells on 75 cm2 tissue culture flasks with vented caps (Falcon 353110) gelatinized with EmbryoMax 0.1% Gelatin Solution (Millipore ES-006-B) for 30 minutes at 37C and plated with 2 million C57Bl/6 mouse embryonic fibroblasts as feeder cells (Gibco A34960 ...
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bioRxiv - Immunology 2020Quote: Tyrosine hydroxylase activity was inhibited by treating B cells with different concentrations of 3-Iodo-L-tyrosine (Sigma-Aldrich, catalogue number: 18250), 30 min before activation with anti-IgM/CpG.
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bioRxiv - Immunology 2023Quote: ... single GC B cells were sorted into wells and 150 μl of supplemented OptiMEM along with 30 μg/ml LPS (Sigma-Aldrich, #L6511) and 4 ng/ml IL-4 (Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Physiology 2022Quote: ... Total protein concentration in the samples was determined with a standard BSA kit (Millipore). 15 µL of the sample was diluted with 60 µL ultrapure water in order to keep the concentration within the linear range of the calibration curve (0.2 to 2 µg⋅µL−1) ...
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bioRxiv - Systems Biology 2020Quote: ... Total protein concentration was measured using bicinchoninic acid assay (BCA Kit, Sigma-Aldrich, USA).
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bioRxiv - Cell Biology 2021Quote: ... was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich). PDMS stamps were incubated with solution containing 50μg/ml fibronectin and 1μg/ml of the conjugated fibronectin in Dulbecco’s phosphate-buffered saline (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: RIP assays employed the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: RIP assay was done with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). Quantitative RT-PCR was used for determining total RNA levels.
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated with the Quantipro BCA assay kit (Sigma-Alrich, United States), and lysates were resolved on a 10% sodium dodecyl sulfate polyacrylamide gel prior to transfer onto polyvinylidene fluoride membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... and membrane fractions were isolated using a Compartment Protein Extraction Kit (Millipore, Billerica, MA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Soluble protein was measured using a Bicinchronicinc acid kit (BCA1, Sigma-Aldrich Canada Co.). Absorbance of each reaction was measured in a spectrophotometer (Spectra Max M2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were detected with an enhanced chemiluminescence assay kit (ECL Plus, Millipore Corporation, USA). Signals were visualized using ImageLab 1.46 (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich, Pool, UK) using bovine serum albumin as standard ...
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bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: ... Protein precipitates were solubilized in 6M urea and subsequently quantified using BCA kit (Sigma). Ten micrograms of the protein samples were reduced with 10mM dithiothreitol (DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... cells were stained using Senescence Cells Histochemical Staining Kit (Sigma #CS0030) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Cell viability monitored using “Cell proliferation kit II (XTT)” from Sigma according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability was assessed using Cell Counting Kit-8 (Sigma-Aldrich), according to the supplier’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was transferred onto a PVDF membrane (Immobilon-P) and detected using mouse anti-c-Myc antibody (Millipore Sigma) as a primary (1:5,000 dilution ...
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bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2019Quote: ... Expression of MxA proteins was detected by Western blot analysis using mouse ANTI-FLAG® monoclonal antibody F4042 (Sigma) and rabbit anti-beta-actin polyclonal antibody ab8227 (Abcam ...
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bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
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bioRxiv - Molecular Biology 2022Quote: ... Nanobody fusion proteins were detected using a primary anti-HA (mouse; 1:3,000, Sigma-Aldrich, St. Louis, MO, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-BRI2 proteins were immunoprecipitated with anti-FLAG mouse monoclonal antibody M2 cross-linked to agarose beads (Sigma A2220); immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799) ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were incubated with primary antibodies (rabbit anti-UPF1, Cell Signaling Technology #12040; mouse anti-G3BP1, Millipore #05-1938 ...
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bioRxiv - Evolutionary Biology 2023Quote: Mouse embryonic stem cell (mESC) cell culture: E14 mESCs (ATTC) were cultured on 0.1% gelatin-coated (Millipore) tissue culture-grade plates in a humidified 37°C incubator with 5% CO2 ...