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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... cells were stained with mouse anti-CD90.1 antibody (Millipore, Bedford, MA) and goat anti-mouse IgG antibody FITC conjugated (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with mouse anti-polyQ (Millipore, MAB1574, 1:50) primary antibody for 2 h ...
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bioRxiv - Cell Biology 2023Quote: Cells or mouse tissues were lysed with TRIzol Reagent (Sigma-Aldrich) and total RNAs were purified according to the company protocol ...
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bioRxiv - Biophysics 2023Quote: ... cells were labeled with mouse anti-α-tubulin (T5168, Sigma-Aldrich) primary antibodies using a 1:500 dilution in 1% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with primary acetylated tubulin mouse antibody (Sigma Aldrich) diluted 1:200 in 1% BSA in PBS and incubated for 1.5 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with mouse anti-FLAG antibodies (Sigma-Aldrich, F1804) diluted (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse ES cells were cultured with 1 mM triptolide (Sigma, T3652) final concentration or equal volume of DMSO only as control for 45 min before EdU labeling ...
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bioRxiv - Cell Biology 2019Quote: ... passed through a 0.450□μm filter and added to ATG2A/B DKO cells in the presence of 10 μg/ml polybrene (Sigma, H9268-5G). Transduced cells were selected by addition of 1 μg/ml Puromycin (iTAP and pHAGE-nTAP-mCherry ...
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bioRxiv - Immunology 2021Quote: ... ICS was performed by incubating cells in 30 μl of antibodies against cytokines and Granzyme B diluted in a Saponin (Sigma-Aldrich) 0.5% W/V solution ...
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bioRxiv - Immunology 2021Quote: ... After washing the B cells with cold PBS and resuspending them in 50 µl cold PBS with 1% bovine serum albumin (BSA) (Sigma #A7979), the cells were first stained with 40 nM of decoy tetramer (10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Relative fold change in DCF signal was acquired by fluorescence spectroscopy and normalised to cell density quantified by 0.04% sulforhodamine B (Sigma-Aldrich, 230162) solublised in 10 mM Trizma (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Biochemistry 2022Quote: ... Lentivirus was generated with pLentiCMV-mitoLbNOX as described above and 143B cells were infected and then selected in 150 µg/mL Hygromycin B (Sigma, H7772) for four days.
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bioRxiv - Biophysics 2022Quote: ... The cells were finally washed with wash buffer B and mounted with Duolink in situ mounting medium with DAPI (DUO82040, Sigma Aldrich). Cells were imaged with Leica TCS SP8 microscope using plan apochromat 40X/1.3 N.A oil objective ...
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bioRxiv - Cell Biology 2022Quote: ... Log phase promastigote cultures (seeded at 2×106 cell/ml) were allowed to grow in complete M199 supplemented with 150 μM biotin (B-4639, Sigma-Aldrich) for 18-24 hours ...
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bioRxiv - Physiology 2021Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatin type B (Sigma-Aldrich). Explants were maintained in RPMI ...
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bioRxiv - Molecular Biology 2022Quote: MHC Class I peptides previously identified to bind HLA-A*03:01 (TIAPALVSK) and HLA-B*35:03 (YPTTTISYL) in HCC38BL cells were purchased as ≥95% pure synthetic peptides from Sigma-Aldrich. These peptides were solubilized in DMSO and used for cell treatments at 20 ug/ml concentration ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were washed with 1mL of 1xPBS prior to adding 4µg/mL of cytokinesis block cytochalasin B (Sigma, C2743-200UL) for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was discarded and the resulting pellet was resuspended with wash buffer (20 mM Tris pH 8, 150 mM NH4Cl, 20 mM MgCl2 supplemented with 1X Cell Lytic B (Sigma-Aldrich). The sample was then spun at 4,000 x g at 4°C for 10 min followed by removing the supernatant and resuspension of the pellet in 4 mL of 50 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2021Quote: ... Tetraploid DLD1 clones were generated by treating diploid cells with 1.5 μg/mL dihydrocytochalasin B (DCB; Sigma Aldrich, St. Louis, MO) for 20 hrs to induce cytokinesis failure ...
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bioRxiv - Immunology 2022Quote: ... using 4.5 mL to transform 8 to 10 million PBMCs in B cell growth medium (Medium A containing CpG [Invitrogen, oligo ZOEZOEZZZZZOEEZOEZZZT] at the 10 μmole scale [desalted], cyclosporin A [Sigma-Aldrich, cat ...
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bioRxiv - Neuroscience 2023Quote: ... then with primary antibodies (anti-LC3 A/B-12741, Cell Signaling, 1:1500 and anti-alpha tubulin-T6199, Sigma, 1:10000), followed by fluorescent secondary antibody incubation ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293T-LP cells with and without integrated libraries were maintained in DMEM supplemented with 50 µg/mL Hygromycin B (Sigma, H3274) and 1 µg/mL Puromycin (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore, H3S28P #07-145 Millipore, VRK1 #HPA000660 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore, H3S28P #07-145 Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... The insulin-secreting cell line INS-1 (a gift from Professor C. B. Wollheim) was cultured in RPMI-1640 (Sigma Aldrich), supplemented with 10% fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Physiology 2023Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatine type B (Sigma-Aldrich). Explants were maintained in RPMI ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The soluble and insoluble protein fractions were extracted using a CelLytic™ MEM Protein Extraction Kit (Sigma–Aldrich, St. Louis, MO, USA) and a CelLytic™ NuCLEAR™ Extraction Kit (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of FLAG-AGO2 was performed with Dynabeads Protein G (Life) and anti-FLAG M2 mouse (Sigma, F3165) for 3h at 4ºC with rotation ...
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bioRxiv - Plant Biology 2019Quote: ... StrepII-tagged proteins were detected using Strep-Tactin AP conjugate (IBA) or a mouse monoclonal StrepII antibody (Sigma). Further primary antibodies used were α-mCherry (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... Lamin proteins were detected using mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR) ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-glial fibrillary acid protein (GFAP) (1/1,000; Sigma-Aldrich, St. Louis, MO, USA; Cat. No. G3893); mouse anti-S100β (1/1,000 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected with anti-γH2A (1:1000) and mouse anti-Ef1α clone CBP-KK1 (1:25,000; Millipore) primary antibodies ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... and SARS2-71 [40] anti-S protein antibodies and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Cell Biology 2020Quote: Antibodies against the following proteins were used at indicated dilutions: anti-acTub (Sigma-Aldrich T7451, mouse 1:2,000), anti-Arl13b (Proteintech 17711-1-AP ...
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bioRxiv - Neuroscience 2021Quote: ... Astrocytes were evaluated by immunostaining for glial fibrillary acidic protein (GFAP; monoclonal mouse anti-GFAP; 1:500; Millipore, Burlington ...
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bioRxiv - Immunology 2021Quote: ... 400 μg protein from the cytosolic lysate was incubated with 5 μg anti-FLAG mouse IgG1 (M2, Sigma) or mouse IgG1 control overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Protein was detected using HRP-conjugated anti-mouse secondary antibody (Amersham) and Luminata Forte Western HRP substrate (Millipore).
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used for protein blotting: mouse anti-c-Myc antibody (Sigma-Aldrich, 05-724MG), mouse anti-Flag antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165, 1:2000), or anti-beta actin (Abcam ...
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bioRxiv - Biochemistry 2023Quote: mAb TnI-1 (IgG1) was purified from mouse hybridoma ascites fluid using Protein G chromatography (Millipore Sigma P7700). TnI-1 IgG eluted with 50 mM glycine-HCl ...
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bioRxiv - Immunology 2024Quote: ... 20 μg cell proteins or 3-5 μg EVs proteins were denatured in LDS sample buffer containing 1M Dithiothreitol (DTT, Sigma) for 10 min at 70°C and loaded onto a 4-12% precast polyacrylamide gels for electrophoresis ...
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bioRxiv - Cell Biology 2019Quote: RAW 264.7 Macrophages: Mouse macrophage cells (hereafter RAW cells) were cultured in DMEM-High glucose (Sigma) supplied with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: Immortalized mouse hippocampal neuronal cells (HT-22) were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA was extracted from tail tissues and amplified with specific PCR primers using REDExtract-N-Amp™ Tissue PCR kit (Cat. No. XNAT-100RXN, Sigma-Aldrich, Saint Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... All transformants were screened by PCR using gene-specific primers (Table S2, Fig. S2c) and were verified by Southern hybridization analyses (DIG DNA Labeling and Detection Kit, Sigma-Aldrich, St. Louis, MO, USA) (Fig ...