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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Estrone 3 Sulfate E1S ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Plates were subsequently blocked with Blocker Casein 1% supplemented with 0.05% Tween 20 (Sigma-Aldrich) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Cells were directly lysed on cell cultures plate with 1 ml TriPure Isolation Reagent (Sigma). After adding 200 μl chloroform and centrifuging at 14,000 rpm for 15 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The plates were diluted to a final drug concentration of 1 mM in DMSO (Sigma Chemical Corporation ...
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bioRxiv - Microbiology 2021Quote: ... After 72 hours the plates were inactivated with 1% Trichloroacetic acid (TCA) (Sigma-Aldrich, UK) washed with water and stained with methylene blue ...
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bioRxiv - Immunology 2021Quote: ... Plates were blocked with a 1% w/v solution of Bovine Serum Albumin (BSA; Sigma) in PBS and serial dilutions of mAbs were added for 1 hour at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Spores were harvested from plates in 1 mL of 0.1% Tween 80 (Sigma, MO, USA) and approximately 108 spores were inoculated into 250-mL flasks containing 50 mL liquid GMM (u-p+r+ ...
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bioRxiv - Genomics 2021Quote: ... Cells were seeded at a 40% confluence on plates coated with Matrigel (1%; Sigma-Aldrich), fibronectin (2 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... were plated on selective MacConkey plates supplemented with 0.5 mM IPTG and 1 % maltose (Sigma). Plates were incubated at 30°C and the colour of the colonies was scored after 40 h ...
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bioRxiv - Neuroscience 2023Quote: ... or 24-well plates were coated using 2.5 mL PDL (1 mg/mL, Sigma P7886) in 47.5 mL borate buffer (100 mM Boric Acid ...
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bioRxiv - Systems Biology 2024Quote: ... or 96 well plates (IC50 studies) pre-treated with a laminin solution (1:100; Sigma) and incubated at 37°C for a minimum of 30 min ...
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bioRxiv - Biochemistry 2024Quote: 1-2 μL from the reaction was spotted on a silica TLC plate (Millipore Sigma) and allowed to dry for a minimum of 15 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... Yeast transformants were selectively grown on SD-Leu (0.17% Yeast Nitrogen Base, 0.5% Ammonium Sulfate, 0.162% Amino Acid-Leucine, [Sigma Y1376], 2% Glucose). YPD and SD-Leu plates contain 2.4% and 2% agar additionally ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μg of protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Millipore, Billerica, MA, USA) and transferred onto a polyvinylidene fluoride membrane (Millipore) ...
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bioRxiv - Developmental Biology 2021Quote: ... Human fibroblasts were doubly transduced with either shRNA or CRISPR vectors in consecutive days in the presence of 8 µg/ml protamine sulfate (Sigma). Transduced cells were selected by puromycin at 1 μg/ml.
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples were separated in sodium dodecyl sulfate (SDS)-PAGE and transferred to polyvinylidene fluoride (PVDF) filter membranes (Millipore, USA) for immune-hybridization ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 min and separated by 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) before being transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were blocked in 5% nonfat milk for 1 h and incubated overnight at 4 °C with the appropriate diluted primary antibodies or anti-flag/GAPDH antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Hybridisation was performed by incubating coverslips with 100 - 250 nM probes diluted in hybridisation buffer (2X SSC buffer, 10% Dextran sulfate (Sigma), 10% Formamide ...
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bioRxiv - Microbiology 2019Quote: ... Tachyzoites were inoculated for 20 hours before a 15 hour stimulation in D1 supplemented with 25mg/ml dextran sulfate (Sigma)60 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were heated and separated on sodium dodecyl sulfate (SDS)-PAGE or Tris-Acetate-PAGE gel-electrophoresis and transferred to PVDF membranes (Millipore). After blocking in phosphate-buffered saline (PBS)/Tween-20 containing 5% nonfat milk ...
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bioRxiv - Systems Biology 2020Quote: ... we pelleted cells by centrifugation at 400 × g for 5 minutes and used low-molecular weight dextran sulfate (Sigma D4911) in hybridization and amplification buffers to improve pelleting.
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bioRxiv - Immunology 2021Quote: ... 106 cells/ml in 500ul of regular medium supplemented with IL-2 500UI in presence of protamine-sulfate at 20ug/ml (Sigma). The plates were then centrifuged at 1,000 g for 1 h and incubated at 37°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... cells were starved with 1 ml of -Met medium without ammonium sulfate for 30 min at 30°C in the presence of 100 μg/ml tunicamycin (Sigma) dissolved in DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... whole cell lysates were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a 0.2μm pore-size nitrocellulose membrane (Millipore-Sigma, Burlington, MA). After transfer ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of total protein were subjected to 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and then transferred to polyvinylidene fluoride (PVDF, Millipore) membranes and incubated with the indicated antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... protein of each sample was separated with 12% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (Millipore). After blocked by 5% fat-free milk (Sangon Biotech ...
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bioRxiv - Biophysics 2020Quote: ... Cells were hybridized with labeled DNA probes (Supplementary Table S2) in the FISH Hybridization buffer (10% dextran sulfate (Sigma D8906) and 10% formamide in 2x SSC ...
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bioRxiv - Biophysics 2022Quote: ... Some samples that were used to obtain or confirm resonance assignments were assembled by mixing protein with equal volume of 1.5 M ammonium sulfate (Sigma-Aldrich), 0.1 M Tris ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1:1000 into a solution of 100 mM sodium acetate/5 mM magnesium sulfate with 26.3 μg/mL calf thymus DNA (Sigma D1501). Assay plate was prepared by aliquoting 190 μL dnaseI assay buffer into 96 round-well clear bottom plates and equilibrating in the dark at 25C ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then fractionated by 12% sodium dodecyl sulfate (SDS)/polyacrylamide gel electrophoresis (PAGE) on polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Immobilon-FL, Millipore). Membranes were blocked with Odyssey Blocking Buffer at room temperature (RT ...
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bioRxiv - Cell Biology 2019Quote: ... #4 inguinal mammary glands were stained for several hours in carmine-alumn staining solution (2% w/v Carmine, Sigma; 5 % w/v Aluminium Potassium Sulfate, Sigma). After the desired color had developed ...
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bioRxiv - Microbiology 2020Quote: ... 30 million eHAP cells were seeded and transduced with gene-trap retrovirus harvested from three combined harvest in the presence of 8 μg/mL protamine sulfate (Sigma). The transduced cells were expanded and passed for a period of 10 days upon transduction ...
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bioRxiv - Immunology 2021Quote: ... equal amounts of cell lysates and immunoprecipitants were resolved by 10-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and were then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore). After incubation with primary and secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and consists of the following components mixed together shortly before use: 10 volumes of 0.25 M zinc sulfate (Sigma-Aldrich), 2 volumes of 36.5-38% (w/v ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418] ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Protein samples were separated by sodium dodecyl sulfate (SDS)-PAGE and transferred onto a nitrocellulose membrane (Millipore, Temecula, CA, USA). After blocking with 5% non-fat powdered milk ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 45-μm polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204 ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then resuspended along with the probes in 15 μl of FISH hybridization buffer (10% dextran sulfate (Sigma-Aldrich), 1 mg/ml E ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were quantified and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). This was followed by immunoblotting with primary antibodies NFATc1 (SAB2101576 ...
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bioRxiv - Microbiology 2019Quote: ... Then 1 × 107 cells were washed with sterile water and transferred to nitrogen starvation conditions [6.7 g/L YNB without amino acids and without ammonium sulfate (Sigma-Aldrich) and 20 g/l glucose] and grown at 30 °C and 200 rpm ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA). The membranes were then incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... Injected embryos were cultured in IVF dishes (Thermo-Fischer 176740) or gelatin-coated dishes in PFSW + penicillin (100 unit/ml) and streptomycin sulfate (0.1 mg/ml) (Sigma P4333A).
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Double fluorescence in situ hybridization was performed using 100 μl hybridization solution (pre-hybridization buffer supplemented with 4% Dextran sulfate (Sigma) containing a combination of two opsin cRNA probes ...
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bioRxiv - Genomics 2021Quote: ... The supernatant was discarded and the cell pellet was carefully ressuspended in 700 μl of sodium chloride-TRIS-EDTA buffer to which 35 μl of 10% sodium dodecyl sulfate (Sigma) and 7 μl of proteinase K at 10 mg/μl (AmpliChem ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... subjected to sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS–PAGE) and electro transferred to polyvinylidene difluoride (PVDF) membrane (Millipore, Germany). The membranes were then blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... for 15 minutes (for forzen sections) or 5 hours (for paraffin sections) and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906 ...
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bioRxiv - Neuroscience 2021Quote: ... Adult animals were placed singly into either 100 ul M9 buffer only or M9 buffer containing 18.5 mM serotonin creatinine sulfate salt (Sigma-Aldrich) in a 96-well microtiter dish ...
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bioRxiv - Cell Biology 2021Quote: ... Stably transfected U2OS cell lines were cultured in the presence of 400 μg/mL Geneticin (G418 sulfate, Sigma, G-3632). RPE-1 ΔCIP2A cell line complemented with lentiviral plasmids were cultured in the presence of 50 μg/mL Nourseothricin dihydrogen sulfate (Roth ...
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bioRxiv - Cancer Biology 2021Quote: ... pellets were dried and solubilized progressively with 5% Sodium dodecyl sulfate in 100mM TEAB solution (Sigma-Aldrich, catalog no. T7408). Total protein quantification was performed using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 8-12 weeks old mice were used and water was replaced with filtered tap water containing 16 g/L paromomycin sulfate salt (Sigma). During the course of infection ...