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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Estrone 3 Sulfate E1S ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... We washed the membrane 3 times with TBST before incubating with a 1:1 ratio of horseradish peroxidase substrate and enzyme mixture (Millipore, Burlington, Massachusetts). We imaged using a Biorad Chemidoc camera set to detect chemiluminescence ...
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bioRxiv - Physiology 2024Quote: ... The rate of O2 change was measured every 13 minutes for a 3-minute interval before sequential challenge with 1) 1 μM oligomycin (Sigma Aldrich 75351), 2 ...
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bioRxiv - Microbiology 2023Quote: ... Blood was mixed at a 1:1 ratio with a filter-sterilized solution containing 3% dextran and 0.9% sodium (Sigma-Aldrich, Burlington, MA). Red blood cells sedimented and fell to the bottom of the tube ...
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bioRxiv - Neuroscience 2024Quote: ... Specimens were then immersed twice in 100% methanol for 1 hour and treated overnight in 1/3 volumes of 100% dichloromethane (DCM; Sigma-Aldrich; 270997). The subsequent day ...
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bioRxiv - Molecular Biology 2024Quote: ... The rate of O2 change was measured every 13 minutes for a 3-minute interval before sequential challenge with 1) 1 μM oligomycin (Sigma-Aldrich: 75351), 2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each mouse was intraperitoneal injected with 3 mL of 3% thioglycolate (Sigma-Aldrich) on day 1 and sacrificed using isoflurane on Day 3 ...
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bioRxiv - Neuroscience 2019Quote: ... Three additional small molecules were introduced on day 3: CHIR99021 (Sigma, 3 µM), SU5402 (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... after approximately 3 days in culture (DIV 3) cytosine β-D-arabinofuranoside (Sigma) was added to the hippocampal cultures in a 50% growth media exchange at a final concentration of 2 µM ...
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bioRxiv - Microbiology 2019Quote: ... and primers p181 (5’-GCGAAGATAACAGTGACTCTA-3’ and p54 (5’-CGGCTCTGATTAAATTCTGAAG-3’) (Sigma, UK).
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bioRxiv - Cell Biology 2019Quote: ... Some PAs were treated with XE991 (3·10-8-3·10-6, Sigma) before the stimulation with 5-HT and then the relaxation induced by SNP was tested.
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bioRxiv - Genomics 2020Quote: ... The cells were bathed in 3-3’ diaminobenzidine tetrahydrochloride (DAB) solution (Sigma Aldrich) during photobleaching.
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bioRxiv - Cancer Biology 2019Quote: ... functionalized with 3-aminopropyltriethoxysilane for 3 min and glutaraldehyde for 30 min (Sigma) and a hydrophobically treated glass slide to generate the even and homogeneous surface of gels on the activated coverslips ...
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bioRxiv - Neuroscience 2023Quote: ... Three additional small molecules were introduced on day 3 (CHIR99021 (Sigma, 3 mM), SU5402 (Sigma ...
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bioRxiv - Immunology 2023Quote: ... 3 mM sodium 3-hydroxibutyrate as GPR81 antagonist (54965-10G-F, Sigma-Aldrich) and 0.5 μM AR-C 141990 hydrochloride (5658 ...
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bioRxiv - Plant Biology 2023Quote: ... in the presence of different concentrations of 3-aminotriazol (3-AT) (Sigma-Aldrich). Quantitative interaction assays were performed in liquid medium by quantifying β-galactosidase activity as previously described (52).
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bioRxiv - Cancer Biology 2023Quote: Butyrolactone-3 (MB-3) was purchased from Sigma-Aldrich (St Louis, MO, USA) and used at a 100µM concentration ...
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bioRxiv - Biochemistry 2024Quote: ... 3-phosphoglyceric phosphokinase and glyceraldehyde 3-phosphate dehydrogenase were obtained from Sigma-Aldrich.
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bioRxiv - Immunology 2024Quote: ELISPOT 96 well plates (MultiScreenHTS IP Filter Plate, 0.45 µm, Sigma-Aldrich) were precoated with 2 µg/ml NP-BSA in PBS over night at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were subsequently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to Immobilon-P polyvinylidene difluoride membranes (IPVH00010; EMD Millipore). Immunoblotting analysis was performed with the indicated antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.5% w/v of dextran sulfate sodium salt, from Leuconostoc spp. with sizes ranging from 6,500 to 10,000, Sigma-Aldrich in PBS). The substrate was aliquoted and stored at –80 °C until required ...
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bioRxiv - Immunology 2019Quote: ... algorithm was used to track the motion of beads and calculate the 3D displacement vector during cell migration compared to a reference position obtained by detaching the cells using 2% sodium dodecyl sulfate (Sigma Aldrich) (34).
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bioRxiv - Cell Biology 2019Quote: ... Medium was removed and cells were incubated for another hour (total infection time: 2 h) at 37 °C in FBS-containing culture medium supplemented with 20 μg/mL gentamicin sulfate (Sigma-Aldrich), to kill extracellular bacteria ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were maintained and cultured using cell culture-grade plastic flasks coated for 2 hours with a mixture consisting of 40 μg/cm2 chondroitin sulfate (Sigma-Aldrich), 40 ng/ cm2 laminin (L4544 ...
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bioRxiv - Neuroscience 2020Quote: 3 ml sterile-filtered washing buffer (Dulbecco’s PBS containing sodium pyruvate, streptomycin sulfate, kanamycin monosulfate, glucose and calcium chloride; Sigma- Aldrich, D4031) containing 0.5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated via sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore, USA) followed by blocking with 5% nonfat milk ...
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bioRxiv - Systems Biology 2020Quote: Frozen or fresh cell pellets (with 106 cells) were denatured using 200ul SDS loading buffer (1x sodium dodecyl sulfate (Sigma-Aldrich), 1x protease inhibitor ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 µg of protein from each sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Merck Millipore, USA). Then ...
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bioRxiv - Neuroscience 2020Quote: ... hyposmia was induced by bilateral intranasal injections with 20 μL of 5% (0.17M) zinc sulfate (Sigma-Aldrich, catalog #7446-19-7) in male C57BL/6N mice ...
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bioRxiv - Cell Biology 2021Quote: Labeling reagents XTT (sodium 3’-[1-(phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) and PMS (N-methyl dibenzopyrazine methyl sulfate) were purchased from Sigma-Aldrich. Assay was performed according to protocol in Cell proliferation kit II (XTT ...
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bioRxiv - Bioengineering 2022Quote: ... GAG content in co-cultured constructs was measured using a dimethyl methylene blue (DMMB) assay [79] with shark cartilage chondroitin sulfate (C4284, Sigma-Aldrich) as a reference ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein samples were electrophoresed on 8% or 10% precast sodium dodecyl sulfate–polyacrylamide gel and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA). The PVDF membranes were incubated with Tris-buffered saline containing Tween (TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... The extracted proteins were analyzed using 5–20% gradient sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) electrophoresis and transferred onto Immobilon Transfer Membranes (Millipore, INYC00010) for immunoblotting ...
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bioRxiv - Immunology 2022Quote: ... Equal amounts of proteins were electrophoresed on 12% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (PVDF, Millipore, USA). PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μg total proteins were loaded to the 8% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gels and transferred onto PVDF membrane (Millipore, IPFL00010) by electrophoresis ...
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bioRxiv - Microbiology 2022Quote: ... Approximately equal amounts of purified histones and parasite histones were separated by 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Millipore, USA). Chemiluminescent detection of bands was carried out by using Super Signal West Femto Maximum Sensitivity Substrate (Thermo Scientific ...
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bioRxiv - Systems Biology 2022Quote: Selected aneuploid and euploid yeast isolates were cultivated in SM medium (6.7 g/L yeast nitrogen base with ammonium sulfate, Difco Cat#291920, 20 g/L glucose, Sigma Cat#Y0626) at 30 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The probe was ethanol- precipitated and dissolved in 10 µL of hybridization mixture containing 50% deionized formamide (Carl Roth GmbH, Karlsruhe, Germany) and 10% dextran sulfate (Sigma-Aldrich).
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bioRxiv - Evolutionary Biology 2022Quote: ... We recovered transformants in a molten SDCAA agarose gel (1.71 g/L YNB without amino acids and ammonium sulfate (Sigma-Aldrich #Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were washed with wash buffer (10% formamide in 1X SSC and 0.1% Tween-20) for 5 min, then incubated with the hybridization mix (10% formamide, 1X SSC, 10% Dextran sulfate (w/v) (Sigma, D8906), 100nM each in situ probe ...
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bioRxiv - Microbiology 2021Quote: ... The purification of virions was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie brilliant blue (Sigma-Aldrich) staining.
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bioRxiv - Neuroscience 2020Quote: ... whole cell lysates were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a 0.2μm pore-size nitrocellulose membrane (Millipore-Sigma, Burlington, MA). After transfer ...
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bioRxiv - Bioengineering 2022Quote: ... Images of fibroblasts were obtained every five minutes for 30 minutes before 100 µL of a 5% w/w solution of SDS (sodium lauryl sulfate, Sigma L3771) was added to remove fibroblasts ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the yeast library was inoculated into liquid SDCAA (1.71 g/L YNB without amino acids and ammonium sulfate (Sigma-Aldrich #Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated for 4-16 h at 37°C with 100 μL/well of virus-specific FISH probe sets (Supplementary Table 4) and primary antibodies in hybridization buffer (10% deionized formamide, 10% dextran sulfate [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2022Quote: ... The cells were incubated with probes diluted to 20 ng/ml in hybridization solution (2XSSC, 50% formamide, 10% dextran sulfate (Sigma, #D8906), 1% Triton ...
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bioRxiv - Immunology 2022Quote: ... Samples were electrophoretic separated on SDS-PAGE gels of sodium dodecyl sulfate-polyacrylamide and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA) at 320 mA for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 150 µl of PBS plus 15 µl of 20% sodium dodecyl sulfate (SDS) and transferred to small vials (200 µl) containing acid-washed glass beads (diameter: 150 – 212 μm) (Sigma-Aldrich). The cells were lysed by bead-beating ...
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bioRxiv - Microbiology 2023Quote: ... Proteins from cell lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (Millipore IPVH00010). For native blots ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
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bioRxiv - Bioengineering 2022Quote: ... content of scaffolds was measured using a dimethyl methylene blue (DMMB) assay [36] with shark cartilage chondroitin sulfate (C4284, Sigma-Aldrich) as a reference ...