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Citations for Millipore Sigma :
1901 - 1950 of 2175 citations for 8 IODOMETHYL QUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... RBC lysis buffer (8% Ammonium chloride [NH4Cl], 0.8% Sodium bicarbonate [NaHCO3, Sigma-Aldrich, USA] and 0.4% Ethylenediaminetetraacetic acid [EDTA, Sigma-Aldrich, USA]).
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bioRxiv - Evolutionary Biology 2023Quote: ... 10 mg of hymenophore tissue from each specimen was homogenized in 2.0 ml screw-cap tubes containing a single 3.0 mm and 8 x 1.5 mm stainless steel beads using a BeadBug™ microtube homogenizer (Sigma-Aldrich, #Z763713) for 120 seconds at a speed setting of 3500 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunoprecipitation was performed using 8 µg of fragmented DNA incubated for 14h at 16°C with 20 µg of S9.6 antibody (Millipore, cat. MABE1095). Next the complexes were captured on ProteinA/G agarose beads (ThermoFisher Scientific R0561) ...
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bioRxiv - Developmental Biology 2024Quote: ... the lysates were separated on 8% v/v or 12% v/v polyacrylamide gels and transferred to a Polyvinylidene difluoride (PVDF) membrane (Millipore ISEQ00010). Membranes were incubated in 5% w/v milk buffer for 1 h at room temperature and incubated in primary antibody overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... quail were placed in glass chambers (3.3 L at 3 weeks; 8.0 L at 8 weeks) ventilated with dried (via drierite; Sigma-Aldrich, Stockholm, Sweden) atmospheric air at least 30 minutes before measurement ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
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bioRxiv - Biochemistry 2023Quote: ... Adherent Expi293 cells were infected with concentrated lentiviral particles at 75% confluency in adherent culture media supplemented with 8 μg/mL polybrene (Sigma-Aldrich), then incubated for 48 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: Clicked and dissolved protein samples were diluted to 4 M urea with 50 mM ammonium bicarbonate (pH 8, AmBic, Sigma-Aldrich). The proteins were reduced with 4 mM DTT (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Dissected tumors were washed in PBS and homogenized at room temperature in urea lysis buffer (8 M urea, 40 mM Tris pH 7.6, 5% SDS supplemented with phosphatase inhibitor cocktails 2, 3 (Sigma P5726, P0044)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Both HCC1954WT and HCC1954KO cells were transduced with the pLenti CMV Puro LUC reporter vector at MOI = 5 in the presence of 8 μg/mL of Polybrene (Sigma-Aldrich). Infected cells were selected by puromycin (2.5 μg/mL for 72h ...
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bioRxiv - Biochemistry 2023Quote: ... Infection of PC3-AR-DTX3LKO cells were carried out in the growth medium (RPMI 1640 + 5% FBS + 1% Penicillin-Streptomycin solution) in the presence of 8 µg/ml of hexadimethrine bromide (Sigma H9268). After 2-3 cell doublings ...
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bioRxiv - Cell Biology 2023Quote: ... 3.5 x 107 K562 cells expressing Cas9-BFP were resuspended into 60 ml of viral media with 8 μg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) in 6-well plates ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with A-RPMI and cultured from day 4 to day 8 in A-RPMI supplemented with 1 μM retinoic acid (Sigma R2625) and 100 ng/ml FGF2 ...
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bioRxiv - Microbiology 2024Quote: ... BEAS-2B cells grown in 6-wells plates were first transduced with lentiviruses encoding Cas9 diluted in infection medium (DMEM, 2% FCS) containing 8 μg/ml Polybrene (Sigma Aldrich). At 16 h post transduction ...
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bioRxiv - Microbiology 2023Quote: ... The normal group was intraperitoneally administered a dose of 8 mL/kg of 0.5% carboxy methyl cellulose (Sigma-Aldrich, MO, USA) as an excipient ...
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bioRxiv - Genetics 2023Quote: ... Experimental Villin-CreERT2 Hnf4 mutant mice (8–12 weeks old) were intraperitoneally administered tamoxifen at 50 mg/kg/day (Sigma, T5648). Wild-type ...
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bioRxiv - Cell Biology 2023Quote: ... elegans genomic DNA extracted from 8-10 adult worms using the Extract-N-Amp™ Tissue PCR Kit (Millipore Sigma, MA) as previously described38 then sequenced ...
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bioRxiv - Genomics 2023Quote: ... Samples were separated on SDS-PAGE (stacking 4% and resolving (8% top and 15% bottom)) and the protein-resolved gel was transferred onto a PVDF membrane (Millipore, #IPVH00010) in a 1X transfer buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were rinsed with PBS and stained according to manufacturer’s protocol (Millipore #BG-6-B, #BG-7-B, #BG-8-C). Embryos were then fixed again in 4% PFA (in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% TritonX100 in 2 × SSC), anti-bleaching buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 3 mM Trolox (Sigma 238813), 0.8% D-glucose (Nacalai Tesque 168060-25) ...
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bioRxiv - Immunology 2023Quote: ... MRC-5/hTERT cells were subsequently transduced with the lentivirus-containing supernatants in the presence of polybrene (8 μg/mL, Sigma-Aldrich) and were selected with puromycin (2 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... virus supernatant was aspirated and preactivated PBMCs (0.5-0.8 x 106/well) in TexMACS medium supplemented with 100 IU/ml IL-2 and 6 ng/ml polybrene (Sigma-Aldrich) were added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... Demyelination was induced in 8-week-old male mice via a 0.2% w/w cuprizone diet (Sigma-Aldrich, cat# C9012-25G). The diet was freshly prepared by mixing cuprizone with standard powder food and water ...
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bioRxiv - Molecular Biology 2024Quote: ... The 1ml of blood was split into two flasks with 30ml of media added to each and delayed until 8 pm on Wednesday at which point dimethyl sulfoxide (DMSO) was added to one flask and rapamycin (Sigma-Aldrich) resuspended in DMSO added to the other at a 1:10,000-fold dilution to give a final rapamycin concentration of 10nM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Stock solutions of CuSO4 (39 mg/L) were prepared by dissolving CuSO4·5H2O (CAS# 7758-99-8; purchased from Sigma-Aldrich) in distilled water ...
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bioRxiv - Cancer Biology 2024Quote: ... The uncured SU-8 was washed away using SU-8 developer and the wafer was silanized using Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma 448931). Sylgard 184 (Ellsworth ...
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bioRxiv - Cancer Biology 2023Quote: ... and then directly added to the intended cells in the presence of 8-10 µg/ml polybrene (TR-1003-G, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2024Quote: ... hPSC were seeded and aggregated in EB formation medium (Essential 8 containing 400 µg/mL Poly (vinil alcohol) (Sigma Aldrich, P8136) and 10 µM Y-27632 (Bio-Connect ...
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bioRxiv - Cell Biology 2024Quote: ... Equal quantities of proteins (20 μg/lane) were loaded onto 8% or 10% SDS-PAGE gels and subsequently transferred to PVDF membranes (Millipore, USA). The membranes were then incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... cells were infected at an estimated MOI of about 1 for each lentivirus in the presence of 8 µg/ml polybrene (EMD Millipore) in 1.5 ml inoculum and incubated at 37 °C for 2 h rocking and rotating every 10 min before adding 8.5 ml of 5 % FBS/DMEM ...
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bioRxiv - Pathology 2024Quote: Nuclei from frozen mouse kidney outer cortex tissue samples and glomeruli-enriched samples were prepared at 4°C.40 Tissue fragments (∼ 8 mm3) were cut by razor blades in EZlysis buffer (#NUC101-1KT, Sigma-Aldrich) and homogenized 30 times using a loose Dounce homogenizer and 5 times by tight pestle ...
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bioRxiv - Cell Biology 2024Quote: ... 3T3-L1 fibroblasts were transduced with titrated viruses in presence of 8 µg/mL of polybrene (Hexadimethrine bromide, Cat. No: 107689-10G, Sigma-ALDRICH) and subjected to antibiotic selection using 4 µg/mL puromycin after 48 hours ...
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bioRxiv - Immunology 2024Quote: ... coated 15μ-Slide 8 well (ibid) and incubated for 2h at 37LJ in RPMI 1640 supplemented with 10% BSA (Sigma-Aldrich). Cells were fixed using 1% [w/v] paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2020Quote: ... At the end of the 3-hour incubation period cells were washed 3x PBS and lysed for western blot in lysis buffer (20 mM Tris pH 8, 10% glycerol, 135 mM NaCl, 0.5% NP40, 2.5 mM MgCl2, DNAse (Sigma-Aldrich, cat# DN25-1G), 1x protease inhibitors (cOmplete Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2019Quote: ... and glucose uptake was initiated by adding 2 ml serum-free and glucose-free DMEM containing 2-[1,2-3H(N)]-2-deoxy-D-glucose (DG) (NET328, specificity 8 Ci/mmol) at 1µCi/ml and 10 mM unlabeled-2-DG (Sigma, Catalog #: D-3179) to each sample in the absence and presence of 20 µM cytochalasin B (Sigma Catalog # ...
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bioRxiv - Developmental Biology 2021Quote: Recombination of the conditional allele was induced in Atxn10flox/flox;CAGG-creERT2 mice at 6 and 8 weeks old by a single intraperitoneal (IP) injection of 9mg tamoxifen (Millipore Sigma, T5648) per 40g (body weight ...
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bioRxiv - Neuroscience 2020Quote: R6/1 mice were treated (from 8 to 20 weeks of age) with vehicle (90% water, 10% Polysorbate 80) or betulinic acid (50 mg/kg; Sigma-Aldrich #855057) administered by oral gavage ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... plants grown under a long day photoperiod (16 h light/8 h dark, 60% humidity) using the SpectrumTM Total RNA extraction kit (Sigma-Aldrich, USA). The sample was enriched for mRNA using the Poly(A ...
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bioRxiv - Genetics 2019Quote: ... Western blots were probed using anti-GFP (Ozyme; #JL-8; 1/1,000) and anti-tubulin (to confirm equal loading) (Sigma, #DM1A, 1/5,000) antibodies ...
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bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
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bioRxiv - Neuroscience 2019Quote: ... treatment consisted of an intraperitoneal injection of either the TrkB agonist 7,8-Dihydroxyflavone (7,8-DHF) (98% Sigma Aldrich CAS#: 38183-03-8) (10mg/kg ...
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bioRxiv - Neuroscience 2019Quote: ... well-fed L3 worms were mounted on 1% agarose pads with 5 mM sodium azide (Sigma-Aldrich, CAS No. 26628-22-8), whereas dauers were mounted on pads with 9 mM sodium azide (Simpkin and Coles ...
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bioRxiv - Bioengineering 2019Quote: ... Hela cells from the same batch of 2D culture were seeded into the peptide hydrogel with density ~ 8 × 104 which was measured by an automated cell counter (Millipore Corporation, PHCC3000). The 3D culture was maintained using a 24-well cell culture plate with ~ 390 μL cell suspension ...
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bioRxiv - Immunology 2019Quote: ... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ...
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bioRxiv - Cell Biology 2020Quote: ... The following day cells were treated for 8 h with either ES DMEM-2i + DMSO or ES DMEM-2i + CHX (100 ug/ml (Sigma-Aldrich, 01810)) ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1-hTERT cells were transduced with the viral supernatant from the transfections in the presence of 8 ug/mL polybrene (Millipore, Burlington, MA) for 24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... the same amount of suspended cells were added into upper chambers of Corning Costar Transwell cell culture inserts (pore size 8 µm; Sigma-Aldrich, #CLS3464) with the coating of 30 μg MatrigelTM Basement Membrane Matrix (BD Biosciences) ...
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bioRxiv - Plant Biology 2021Quote: ... mixtures consisted in 2 μl of diluted template and 8 μl of FastStart Universal SYBR Green Master (Rox) (Cat. # 04913914001, Sigma-Aldrich Argentina). Reactions were set in technical duplicates or triplicates in MicroAmp® Fast Optical 48-well 0.1 mL reaction plates (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... animals at 8 weeks of age were gavaged with 100 µL of a 30 mg/mL solution of tamoxifen/corn oil (Sigma Aldrich T5648). This was done 3 consecutive days then animals were allowed to rest for 4 days before another three consecutive daily doses (6 doses over 10 days) ...