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Citations for Millipore Sigma :
1701 - 1750 of 2175 citations for 8 IODOMETHYL QUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Synthetic neotetraploids were created from six genetically distinct diploids using the mitotic inhibitor colchicine (Sigma Aldrich, CAS: 64-86-8). Details of the methodology are found in Anneberg et al ...
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bioRxiv - Genomics 2023Quote: ... cells were transduced with the GFP-pilot lentiviral supernatant supplemented with 8 μg/ml polybrene (Sigma-Aldrich TR-1003-G) at MOI ∼0.1 ...
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bioRxiv - Immunology 2024Quote: ... 1.4×108 human primary CD4+ T cells were transduced with lentivirus at a multiplicity of infection of 2 with 8 µg/ml dextran (Sigma). Virus and DynaBeads were removed after 3 days of transduction ...
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bioRxiv - Genomics 2024Quote: ... The spinfection was carried out at room temperature for 30 minutes spinning at 1000g and the cells were supplemented with 8 μg/ml polybrene (hexadimethrine bromide, Sigma). 2 µg/ml puromycin was added to the cells 24 hours post infection ...
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bioRxiv - Biophysics 2024Quote: ... Gene expression was induced at OD600 ∼0.8 by addition of either 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG; Carl Roth GmbH) or 0.2 µM anhydrotetracycline (Sigma). Cells were grown overnight at 22°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 17 β-estradiol was added to the drinking water twice a week at a final concentration of 8 μg/mL (cat# E2758; Sigma). CHK2 activator (3 ...
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bioRxiv - Systems Biology 2024Quote: ... 2M thiourea, 100mM Tris-HCl, pH 8, 150mM NaCl, 1mM EDTA, phosphatase inhibitor cocktail 2 and 3, 10mM NaF; Sigma), reduced with 10mM DTT (dithiothreitol ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were fractionated using SE250 Mighty Small II (Hoefer Scientific Instruments) on SDS-PAGE minigels (1.5 mM thick, 8% polyacrylamide) and transferred to Immobilon-P membranes (Millipore IPVH00010) using a Bio-Rad Mini Trans-Blot (#1703930 ...
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bioRxiv - Cell Biology 2024Quote: ... Retroviral supernatant containing various C/EBP constructs was added to dKO-B cells together with 8 ug/mL polybrene (Sigma). Cells were infected by spinoculation at 2000 rpm at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... leaf pieces were transferred onto solid (0.8 % phyto agar [Duchefa]) MGI medium (full-strength MS medium containing 1.6 % glucose [Roth], 1 mg/l BA [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: ... The viral supernatant was filtered through 0.45 µm filter and added to the target cells along with 8 µg/ml polybrene (Sigma, H9268). Following one to three infections ...
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bioRxiv - Molecular Biology 2024Quote: ... protein was loaded onto a 10% or 8% SDS-PAGE gel and was transferred to Immobilon-FL PVDF membrane (IPFL00005, Millipore). The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041 ...
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bioRxiv - Developmental Biology 2024Quote: ... the Epiblast was separated from the Visceral Endoderm by 8 minutes incubation on ice in a solution of 2.5% pancreatin (Sigma, P3292) and 0.5% trypsin (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... and 25 mM Tris pH 8) and concentrated with centrifugal concentrators (10 kDa MWCO, Millipore Sigma-Aldrich, Burlington, MA, USA) to a final volume of 0.1 mL ...
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bioRxiv - Cell Biology 2024Quote: ... were resuspended at cell densities of 8×106 cells/mL for each cell type into a hydrogel comprised of 6.5mg/mL fibrin (Sigma #341573) and supplemented with 0.2mg/mL fibronectin (Sigma #F1141 ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Cancer Biology 2024Quote: ... zebrafish larvae (8-9 per condition) were dissociated into single cells by applying enzymatic digestion using 5µl of liberase (Sigma-Aldrich) (2.5mg/ml ...
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bioRxiv - Biophysics 2024Quote: The purified protein was concentrated to 5–8 mg/ml using a 50 kDa molecular weight cutoff (MWCO) centrifuge concentrator (Millipore) and loaded in 0.5 mL portions onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... were separated by 8 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE) and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore) at 120 V for 2 hours using transfer buffer (25 mM Tris-base ...
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bioRxiv - Biophysics 2021Quote: ... batch number: SLCC4853) and poly(ethylene glycol) (PEG 8000, Mv 8 kg/mol, batch number: MKBT7461V) were purchased from Sigma-Aldrich. 1 ...
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bioRxiv - Cell Biology 2020Quote: ... to cells together with polybrene transfection reagent at a final concentration 8-10 mg/mL (Cat# TR-1003-G, EMD Millipore). 48 h post-infection ...
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bioRxiv - Cell Biology 2020Quote: ... a polycarbonate filter (8-μm pore size) (GE Infrastructure, Fairfield, CN, USA) was coated with a solution containing 0.2% gelatin (Sigma-Aldrich Co.) and inserted between the chambers ...
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bioRxiv - Cell Biology 2020Quote: ... streptavidin-sepharose beads were resuspended in 200 µl of 50 mM ammonium bicarbonate pH 8 containing 1 µg of trypsin (Sigma-Aldrich). Samples were then incubated at 37°C overnight with mixing on a rotating disc ...
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bioRxiv - Cell Biology 2019Quote: ... perm-1 adult worms were dissected in 8-10 µL of 62% ESF 921 with 15 µm polystyrene beads (Sigma-Aldrich) on a 22 x 50 mm coverslip ...
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bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... 12-well plates of 293T or HUVEC cells were incubated with viral inoculum diluted in antibiotic and serum free DMEM containing 8 μg/mL hexadimethrine bromide (polybrene; Sigma-Aldrich). Plates were centrifuged at 800 x g for 2 hours at room temperature (61) ...
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bioRxiv - Microbiology 2019Quote: ... niger cultures grown at 28°C for 7-12 days on PDA plates were harvested using 10 ml saline tween solution (8 g l-1 NaCl and 0.05 ml l-1 Tween 80) and filtered through Miracloth (Millipore; Billerica, USA) following the protocol described in [21] ...
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bioRxiv - Developmental Biology 2021Quote: ESCs were infected with an MOI=1 for 16 hours in ESCFBS conditions supplemented with polybrene at 8 µg/mL (Sigma-Aldrich). 24h after infection ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were washed once with Advanced DMEM and resuspended in a transduction medium (ENR media supplemented with 1mM nicotinamide, Y-27632, Chir99021, 8 μg/ml polybrene (Sigma-Aldrich)) and mixed with the previously collected concentrated virus ...
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bioRxiv - Cell Biology 2022Quote: ... AKAP450 and AKAP450/CAMSAP2 knockout RPE1 cells were infected with lentivirus and incubated in complete medium supplemented with 8 μg/ml polybrene (Sigma-Aldrich). After 24 hrs ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were electrophoresed on 8% (LRRK2) or 15% (α-synuclein) SDS-PAGE gels and transferred overnight onto PVDF membranes (Millipore). Blots were either blocked with Everyblot blocking reagent (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml deionized water, 8 mg of sucrose (A2188.1000, Axon Lab, Switzerland) and 1 mg of Amaranth dye (A1016, Sigma-Aldrich, USA), (ii ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were washed once with Advanced DMEM and resuspended in transduction medium (ENR media supplemented with 1mM nicotinamide, Y-27632, Chir99021, 8 μg/ml polybrene (Sigma-Aldrich) and mixed with concentrated virus ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 °C and suspended in resuspension buffer (50 mM Tris-HCl, pH 8, 300 mM NaCl, 2 mg/ml lysozyme (Sigma, L6876), 1 mM EDTA ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was thawed on ice and resuspended in 50 μl SLB/c buffer (10 mM Tris-HCL pH 8, 10 mM EDTA, 1% SDS supplemented with protease inhibitor (11836170001, Sigma-Aldrich)) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Molecular Biology 2019Quote: ... in second attempt we used the library consisted of 8 random nucleotides with fixed G in the middle position: 5’-GCTCAAGCTTACTGCAGATATNNNNGNNNNTTTAGGATCCATCTAGAGTCCGA- 3’(SIGMA-ALDRICH). 15µg of the library were made double-stranded with 5µg 3’ primer (CAST1_R- ACTCTAGATGGATCCTACG or CAST2_R-TCGGACTCTAGATGGATCCTA ...
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bioRxiv - Bioengineering 2019Quote: ... 200 μL of folded reaction was purified from excess staples and buffer exchanged into 20 mM Tris-HCl pH 8.0 and 8 mM MgCl2 using a 100kDa MWCO spin concentrator (Amicon, Merck Millipore, Billerica, MA). The concentration was subsequently adjusted to 5 nM ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 40 ml of Buffer A (50 mM Tris-HCl pH 8, 500 mM NaCl) in presence of cOmplete™ EDTA-free protease inhibitor (Sigma) and lysed using an EmulsiFlex-C3 homogenizer (Avestin) ...
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bioRxiv - Cancer Biology 2019Quote: ... The membrane was UV-crosslinked for 3 minutes/each side and hybridized with probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) and developed as described62 ...
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bioRxiv - Cancer Biology 2019Quote: ... the gel was blotted and probed with antisense telomere probe (CCCTAA)8 labelled with DIG Oligonucleotide 3’-End labeling Kit (Sigma-Aldrich) or with a 1.6 kb fragment containing the sequence (TTAGGG)n62 ...
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bioRxiv - Biochemistry 2019Quote: ... 43-48 h after induction, cells were harvested and resuspended in buffer A (50 mM Tris pH 8, 150 mM NaCl, 1% digitonin (Sigma-Aldrich), 6.4 mM β-mercaptoethanol ...
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bioRxiv - Cell Biology 2019Quote: ... L-lysine-13C6, 15N2 (Lys-8, #608041)) and L-arginine-13C6, 15N4 (Arg-10, #608033) stable isotope labelled amino acids (Sigma-Aldrich). Media was also supplemented with 10% dialysed FCS (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... and lysed in solubilization buffer (50 mM Tris–HCl pH 8, 1% Igepal and 1 mM EDTA) with protease inhibitor cocktail (Sigma Aldrich). The cell lysate was centrifuged at 14,000×g for 20 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Supernatants containing lentiviral particles were used to infect MDA-LM2 cancer cells overnight in the presence of 8 μg/ml polybrene (Sigma-Aldrich). Infected cells were selected with 2 μg/ml puromycin (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... U937 cells were transduced by spin infection in which cells were suspended in viral media with 8 μg/mL polybrene (Sigma Aldrich), centrifuged at 1000 x g for 1 hour ...
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bioRxiv - Plant Biology 2020Quote: ... The fixative was washed away with phosphate buffered saline buffer supplemented with Tween 20 (PBST) and cells were treated for 8 min at room temperature with a solution of 2% (w/v) Driselase (Sigma-Aldrich) in 0.4 M mannitol ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 8–20 mg/mL (Amicon Ultra 10 kDa cut off, Millipore Sigma). Protein samples were stored at 4°C until use.
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bioRxiv - Bioengineering 2021Quote: PBMC-iPSCs were transduced overnight with lentiviruses that co-express dCas9-VPR and the puromycin resistant gene (EF1a-dCas9-VPR-P2A-Puro) in the presence of 8 µg/mL of polybrene (Sigma-Aldrich). Two days post-transduction ...
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bioRxiv - Bioengineering 2020Quote: ... The cell culture medium was replaced with 2 mL fresh medium containing 8 µg/mL hexadimethrine bromide (Sigma Aldrich, H9268-5G), 20 µL ViralPlus Transduction Enhancer (Applied Biological Materials Inc. ...