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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for Rat TGF beta 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Rats received either a 30-minute infusion of corticosterone (0.75 mg/mL Corticosterone-2-hydroxypropyl-β-cyclodextrin; Sigma-Aldrich, Gillingham, UK) dissolved in sterile 0.45% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 8 dpi and ending at 28 dpi.
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bioRxiv - Microbiology 2024Quote: The experimental plate for HepG2/C3A cells was prepared by coating with 100 µg/mL of rat tail collagen (Sigma-Aldrich) and 0.02 M glacial acetic acid in PBS for a minimum of 2h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was started after 5 min preincubation by adding 13 µL of a microsome mix from the liver of Sprague-Dawley rats (Sigma Aldrich, 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Neuroscience 2023Quote: ... thoracic and lumbar dorsal root ganglia neurons were extracted from E17.5-E18.5 rat embryos and plated on glass coverslip coated with poly-L-ornithine (Sigma-Aldrich, MO, USA) for 4-6 hours before experiments.
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bioRxiv - Neuroscience 2023Quote: ... After 1-2 months old rats undergo trans cardiac perfusion with PBSx1 (02-023-5A, Biological Industries) followed by neutral buffered 10% formalin (HT5011, Sigma-Aldrich). The whole brain was removed and immersed in 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were dissected from postnatal Sprague Dawley rats (P0-P2) and dissociated with 0.25% trypsin (Sigma Aldrich, Merck, Darmstadt, Germany) and 0.004% DNAse (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: Decellularized rat lung and isolated lobes were perfused with a solution of 0.1% Methylene blue solution (Cat. # 03978, Sigma Aldrich, UK) in PBS either from the trachea or pulmonary artery ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse and rat kidneys were fixed by perfusion with 50-60 mL of 4% paraformaldehyde in PBS (158127, Sigma-Aldrich), dehydrated and embedded in paraffin at 58C ...
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bioRxiv - Immunology 2023Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 10 dpi and ending at 34 dpi.
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bioRxiv - Molecular Biology 2023Quote: ... mice and rats (male Sprague-Dawley, aged 7-10 weeks) were subcutaneously injected with the VEGF-receptor inhibitor SU5416 (Sigma-Aldrich) at a dosage of 20 mg/kg body weight under isoflurane anesthesia ...
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bioRxiv - Cell Biology 2024Quote: ... For transfection of human or monkey keratinocytes tissue culture plastic vessels were coated with 20 µg/ml rat Collagen I (Sigma, C3867) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with primary antibodies (1:200) which were diluted with Immunoreaction Enhancer Kit (Millipore sigma) overnight at 4°C in a humid chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... 350 μl RLT buffer (from the AllPrep DNA/RNA Micro Kit) containing 1% β-Mercaptoethanol (Sigma-Aldrich) was added to each sample (containing one biopsy ...
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bioRxiv - Genomics 2022Quote: ... The tissue was gently Dounce homogenized in 500 µL of Nuclei Isolation Buffer (Sigma Nuclei PURE Prep Kit: Lysis Buffer, 1 mM DTT, Sigma Nuclei PURE Prep Kit: 10% Triton X-100). After a brief centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... were maintained at 37°C on rat collagen diluted at 100µg/mL in water (Cultrex; catalog no. 3443-100-01) in EndoGro medium (Merck Millipore; catalog no. SCME004) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated at 14 °C (a temperature not permissible for recycling) with unconjugated goat anti-rat IgG (Sigma, 4 μg/ml) to block labelled channels remaining at the cell surface ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated with the primary antibody (rat anti-serotonin, Chemicon MAB352, 5/1000; rabbit anti-tyrosine hydroxylase, Sigma Aldrich SAB2701683, 2/1000) in buffer (1% sheep serum ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Female rats were administered an intraperitoneal injection of “Hellabrunner Mischung” comprising ketamine hydrochloride/xylazine hydrochloride solution (1ml/kg, Sigma Aldrich, Taufkirchen, Germany). The surgical procedure followed the description of Spicer and colleagues (44) ...
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bioRxiv - Physiology 2020Quote: DN was induced experimentally in 12-h fasting rats except for the normal group through a single intraperitoneal injection of 0.5 mL STZ (Sigma-Aldrich, Deisenhofen, Germany) at a dosage of 60 mg/kg of body weight ...
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bioRxiv - Neuroscience 2021Quote: ... (2) rats underwent UVN and received intraperitoneally one L-thyroxine (L-T4) injection (10 µg/kg, dissolved in saline, Sigma, Cat#T2501) at the end of the surgery and one injection/day during the first 3 days post-lesion (UVN-T4 group ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Pathology 2024Quote: ... The diabetic rats in the DM+GR group were given a standard diet and were also given glyburide (Sigma-Aldrich; Merk KGaA) at 4 mg/kg in 0.5 ml of 0.5% Tween 80 solution as a supplement ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad], Secondary anti-Rat IgG, [ThermoFisher], and anti-NeuN [Millipore Sigma] ...
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bioRxiv - Immunology 2023Quote: ... and subsequently incubated with anti-CD16/CD32 (eBioscience, Cat #14-0161-86, clone 93) and 10% rat-IgG1 (Sigma-Aldrich, Cat #I8015) in PBS containing 0.1% BSA (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... The phosphatase assay was carried out using the Ser/Thr Phosphatase Assay Kit 1 (Millipore Sigma (MA, USA)) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Nuclear and cytoplasmic extracts were prepared from differentiated THP-1 cells using Nuclei EZ Prep kit (Sigma-NUC101) both from un-stimulated and MprA stimulated cells ...
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bioRxiv - Genetics 2020Quote: Cells were crosslinked with 1% formaldehyde for 10min before chromatin fragmentation with EZ-enzyme kit 17-375 (Millipore) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of the supernatant was then mixed with 4 µL of glucose assay kit (Sigma GAHK20-1KT) and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of each sample was then mixed with 4 µL of glucose assay kit (Sigma GAHK20-1KT) and incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was isolated from 1 million cells in triplicates using GenElute Mammalian Total RNA Miniprep Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... allowing for detection of the secondary HRP antibody detected using a chemiluminescence peroxidase substrate kit (Sigma, #CPS-1) and subsequent detection of the GAPDH loading control detected using its laser based infrared detection system ...
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bioRxiv - Microbiology 2022Quote: ... or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). Normalised DNA (at a concentration of 0.2 ng/ml ...
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bioRxiv - Cell Biology 2020Quote: ... dark agouti rats via a single intraperitoneal injection of streptozotocin (STZ, 55 mg/kg, in trisodium citrate buffer, pH 4.5, Sigma-Aldrich Co, MO, USA), with control animals receiving an equivalent volume of vehicle ...
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bioRxiv - Neuroscience 2021Quote: Sprague Dawley male rats received four ip injections of 4 mg/kg methamphetamine hydrocholoride (dissolved in 0.9% saline Sigma, St Louis, MO, USA) every two hours for 8 hours with rectal probe body temperature measurements every hour ...
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bioRxiv - Neuroscience 2020Quote: ... Rat pups received oral administration of fluoxetine (10 mg/kg; kind gift from IPCA Laboratories) or vehicle (5% sucrose, Sigma-Aldrich, Cat no.) once daily through a micropipette (0.5-10 μL ...
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bioRxiv - Physiology 2021Quote: ... Cell pellets were resuspended in growth medium and plated in tissue culture dishes coated with type I rat tail collagen (Sigma, St. Louis, MO). Cultures were expanded and frozen at passage 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The tube was kept at 37 °C for 10 min before a 13 μL aliquot of the Sprague-Dawley rat liver microsome (Sigma-Aldrich, No. M9066) was added ...
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bioRxiv - Bioengineering 2020Quote: The effect of HMGB1 on inducing the migration of rat BMSCs were examined using a Trans-well plate (Millipo Sigma, St Louise, USA). The rat BMSCs in DMEM at passage 1 were seeded into the upper layer of insert well of the trans-well plate ...
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bioRxiv - Neuroscience 2022Quote: ... recordings were performed with borosilicate glass pipettes (7-15 MΩ) and filled with normal rat Ringer’s solution with 2% biocytin (Sigma, cat # 576-19-2). The pipette was positioned with a micromanipulator (Luigs & Neumann ...
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bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence was performed according to standard procedures with antibodies against the N-terminus of the laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), the laminin α1 chain (rat monoclonal ...
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bioRxiv - Genomics 2023Quote: ... Rats received a single oral gavage administration of freshly prepared 40 mg/kg N-ethyl-N-nitrosourea (ENU) (Sigma Aldrich, St. Louis, MO) dissolved in PBS (Nova-Tech ...
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bioRxiv - Biophysics 2024Quote: ... measurements of the identified ChRs were expressed in a mouse neuroblastoma and rat neuron hybrid cell line (ND7/23 cells, ECACC 92090903, Sigma-Aldrich, Munich, Germany). Cells were cultured as previously described (Grimm et al. ...
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bioRxiv - Plant Biology 2024Quote: ... Kit (Sigma). RNA was treated with TURBO DNAse (Thermo ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked for 1 hour in 1% BSA/TBS-T and probed with antibodies provided in the kit according to the manufacturer’s instructions (Millipore).
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bioRxiv - Molecular Biology 2021Quote: ... 1/1000) antibodies for 2 h at 37 °C in antibody diluent solution (Duolink in situ kit, Sigma-Aldrich). The cells were then labeled with the PLA antimouse PLUS probe (DUO92001 ...
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bioRxiv - Cancer Biology 2022Quote: The viability of THP-1 cells was assessed for 24h using the Cell Proliferation Kit II (XTT) (Sigma-Aldrich) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... each RNA sample was supplemented with 1 unit of DNase I (from the DNA amplification grade Kit of Sigma) per µg of RNA and incubated at room temperature for 10 min ...
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bioRxiv - Pathology 2020Quote: ... Adiponectin was measured in the samples using MILLIPLEX Mouse Cytokine/Chemokine 1-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Cleared lysates were obtained after centrifugation for 1 hour at 20,000 x g and incubated with anti-FLAG M2 affinity gel (FLAG Immunoprecipitation Kit, Sigma). Bound proteins were eluted with 3xFLAG peptide solution.