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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for Rat TGF beta 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Nuclei from the 700mg of wildtype flower tissue were isolated using the Plant Nuclei isolation kit (Sigma, # CELLYTPN 1) conferring to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Samples of culture were adjusted to OD600 0.5 mL−1 and processed according to the manufacturer’s instruction ADP/ATP Ratio Assay Kit (Sigma-Aldrich). For NADH/NAD+ quantification samples from WT and ΔmftG grown in triplicates on HdB-Tyl supplemented with 10 g L−1 glucose for 24h were also quantified ...
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bioRxiv - Cancer Biology 2023Quote: ... Fixed cells were transposed using barcoded Tn5 (Seqwell) in a transposition buffer (1 × TD buffer from Illumina Nextera kit, 0.1% Tween 20 (P9416, Sigma), 0.01% Digitonin (G9441 ...
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bioRxiv - Immunology 2023Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol7,11 ...
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bioRxiv - Immunology 2023Quote: ... or LIVE/DEAD™ Fixable Blue Cells Stain Kit (#L34957, Thermo; 1:1000 in PBS) and fixation for 15 minutes at RT using 1.85% formaldehyde (F1635, Sigma) in PBS solution before surface staining with antibodies in PBS supplemented with 0.5% BSA [fraction V ...
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bioRxiv - Cell Biology 2023Quote: ... retinoic acid and epidermal growth factor (EGF) as per the SAGM Bullet Kit and 0.1 μg ml−1 cholera toxin (cat. no. C8052, Sigma), 5% FBS and 1% Pen/Strep ...
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bioRxiv - Microbiology 2024Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore) using Liga5™ (A&A Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... The lactate assay kit (Lactate Assay kit, Sigma Aldrich) was used to measure L (+)-Lactate in the culture media according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... pregnant rats were randomly assigned to be intraperitoneally injected with DHT (1.66 mg/kg/day, suspended in sesame oil, Sigma-Aldrich, St. Louis, MO, USA) and/or human recombinant INS (6.0 IU/day ...
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bioRxiv - Bioengineering 2021Quote: ... using a Milliplex Rat Gut Hormone Panel 96-well plate assay as described by the manufacturer (#RMHMAG-84K-06; EMD Millipore Corporation, Billerica, MA). Data was collected with MAGPIX with xPONENT software (Luminex ...
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bioRxiv - Neuroscience 2021Quote: ... corresponding to the external Ig-like domain) diluted in sterile PBS and emulsified in an equal volume of incomplete Freund’s adjuvant (IFA, Sigma; total volume 100µl per rat). IFA control rats were immunised with PBS emulsified in an equal volume of IFA ...
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bioRxiv - Neuroscience 2020Quote: ... Cells (primary culture of rat ENS) and tissues from mice (hippocampus, ileum, proximal and distal colon) were lysed in RIPA lysis buffer (Merck Millipore, Fontenay sous Bois, France) containing phosphatase inhibitor cocktail II (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST before incubating overnight at 4°C in primary antibody solution (4% NGS in TBST) with rat anti-GFAP (Millipore Cat# 345860-100UG, RRID:AB_211868), rabbit anti-GFAP (1:2000 ...
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bioRxiv - Physiology 2020Quote: ... The hydrolyzed TMOS (100 μl) was added to 900 μl of 10 μM of pooled siRNA against rat FL2 (siRNA from Sigma-Aldrich (St Louis, MO): SASI_Rn02 00314854 ...
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bioRxiv - Physiology 2021Quote: ... the experimental rats (body mass 171 to 203 g) were injected subcutaneously with 60 mg monocrotaline in saline/kg body mass (Sigma-Aldrich, St Louis, MO) to induce pulmonary hypertension ...
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bioRxiv - Bioengineering 2022Quote: ... As previously described,29 male Sprague Dawley (SD) rats (200-250g) were injected with monocrotaline (MCT) (60 mg/kg, Sigma Aldrich, Oakville, ON, Canada) by intra peritoneal (i.p. ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were loaded on an 8% Tris/glycine gel and subjected to Western blot analysis using different primary antibodies (anti-c-myc (A-14, sc-769, Santa Cruz, Nunningen, Switzerland and HA antibody, rat, 3F10, Sigma-Aldrich, Munich, Germany).
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bioRxiv - Neuroscience 2022Quote: Primary rat glial cells (astrocytes and microglia) and neuroblastoma cells were fixed for 15 min in 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) and washed with PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Four to five-week-old donor rats were superovulated by intraperitoneal injection of pregnant mare serum gonadotropin (30 units, G4877, Sigma-Aldrich, St. Louis, MO), followed by an intraperitoneal injection of human chorionic gonadotropin (30 units ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Plasma and dorsal hippocampal CA1 cytokines were assessed using a MILLIPLEX® Rat Cytokine/Chemokine Magnetic Bead Panel (Millipore Sigma Cat. No. RECYTMAG-65K) customized for detection of pro-inflammatory (TNF-α ...
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bioRxiv - Microbiology 2023Quote: ... All well plates and culture flasks were coated prior to seeding with 7 µg/mL rat tail tendon collagen I (Sigma-Aldrich, St. Louis, MO) and rinsed with PBS 3 times ...
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bioRxiv - Neuroscience 2023Quote: All plates required for cell culture were coated with 100µg/mL with collagen rat tail type I (20nM acetic acid/distilled water) overnight before seeded with cells (Sigma-Aldrich; UK, ThermoFisher Scientific; USA). Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mL/well cell-virus suspension was seeded into 50 µg/mL rat tail collagen type I (Sigma Aldrich, St. Louise, MO USA) coated 6-well plates ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from 1 x 106 stable cells using the GenElute mammalian genomic DNA kit (G1N350, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... A tertiary streptavidin-HRP antibody (a part of the Mouse ExtrAvidin® Peroxidase Staining Kit from Sigma at 1:1000) was added for 2 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
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bioRxiv - Pathology 2019Quote: ... treated with opsonized RBCs (1:10) and intracellular ROS was determined with a fluorometric intracellular ROS kit (Sigma-Aldrich, MAK142). Stained cells were visualized and captured using Leica DMI6000B with Cy5 filter set ...
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bioRxiv - Biochemistry 2020Quote: ... revVS and shVS were purified from cultures of transformed Escherichia coli C41(DE3) cells that were induced with the Overnight Express Autoinduction Kit System 1 (Novagen). Cells were pelleted and resuspended in Buffer A containing 10mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Plant Biology 2021Quote: The coding sequences of most of the 44 non-pseudogene AtUMAMIT were amplified by PCR (oligonucleotide sequence is provided in Supplemental Table 1) by using the KOD Hot Start DNA Polymerase kit (Novagen) from a mix of reverse-transcribed RNA extracted from seedlings and inflorescences ...
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bioRxiv - Genomics 2020Quote: CTCF Chromatin immunoprecipitation (ChIP) was performed on 1 × 107 Ter119+ erythroid cells using a ChIP Assay Kit (Millipore: 17-295) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 1×105 cells were used to measure NADPH and NADP+ levels using a NADP+/NADPH colorimetric quantitation kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2019Quote: ... or doxycycline-treated (Dox) (1 µg/ml for 6 days) AW13516-shNPM1-luc+ cells using the GenElute Mammalian Total RNA Miniprep Kit (Sigma) followed by On-Column DNase I Digestion as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... referred to here as hCMEC were obtained from Millipore and were maintained in an EndoGRO-MV complete medium kit supplemented with 1 ng/ml fibroblast growth factor-2 (FGF-2; Millipore) (81–84) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cloning was performed according to the manufacturer’s protocol using the T7Select® 415-1 Cloning Kit (Millipore, 70015-3) with 1 μL of annealed oligonucleotides.
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bioRxiv - Neuroscience 2019Quote: The anterior half of cortices from P0 mice were dissociated then cross-linked with 1% formaldehyde and processed using EZ-ChIP kit (Millipore). Chromatin fragments bound by endogenous mouse SOX5 were pulled down by anti-SOX5 (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Viral titer was determined by LV Lentiviral Titer kit (MoBiTec) and viruses where used at a Multiplicity of infection of 1 – 5 using polybrene (H9268, Sigma) at a concentration of 2 μg/ml.
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bioRxiv - Bioengineering 2022Quote: ... These oligonucleotides were used to create a phage library representing all oligopeptides using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol (Harhala et al. ...
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bioRxiv - Pathology 2022Quote: ... The incubation was followed by three 5 min washes and incubation with PLA probes anti-goat PLUS (DUO92003) and anti-rabbit MINUS (DUO92005) for 1 h at 370C as recommended in the Duolink PLA kit (Sigma). After three washes and cells were incubated with the hybridization solution containing DNA ligase for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... The abundance of cytokines was determined using a MILLIPLEX Mouse 32-Plex Cytokine Panel 1 kit (Millipore # MCYTMAG-70K-PX32) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Sodium kit: Sodium Assay Kit (Colorimetric) (Sigma, product code: MAK247). Potassium kit ...
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bioRxiv - Physiology 2022Quote: ... NS in rats was induced by a single intra-jugular injection of 150 mg/kg body weight of puromycine-aminonucleoside (PAN) (Sigma–Aldrich, Saint Louis, MI, USA). Control rats received a single injection of 1 ml /100 g body weight of isotonic NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... rats were anesthetized with 100 mg/kg ketamine (Midwest Veterinary Supply, Valley Forge, PA) and 10 mg/kg xylazine (Sigma-Aldrich/RBI, St. Louis, MO). An indwelling catheter (SAI Infusion Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... ECL kit (Millipore) was used to display target protein bands.
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bioRxiv - Microbiology 2021Quote: ... were fluorescently labelled by the addition of 2 μl PKH26 dye (1 mM stock concentration) from the MINI26 PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich) and mixed by pipetting ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed in 70% ethanol for 1 h and stained for 10 min with an ALP staining solution (BCIP/NBT alkaline phosphatase kit, Sigma-Aldrich), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Bacteria were lysed by bead-beating in the presence of buffer RA-1 (NucleoSpin RNA isolation kit, Mackery-Nagel) and β-mercaptoethanol (Sigma). RNA isolation and purification was continued according to the protocol supplied by the manufacturer (Nucleospin RNA isolation kit ...
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bioRxiv - Developmental Biology 2019Quote: ... H9 hESCs were nucleofected using the Lonza P3 Primary Cell 4D-Nucleofector X Kit and monoclonal colonies were selected for 7-10 days with 1 μg/ml of puromycin (Sigma-Aldrich). Tetracycline hydrochloride (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: RNA isolation was carried out with 2 g of soil using RNA PowerSoil Total RNA Isolation Kit (Mo Bio Laboratories, Carlsbad, CA. USA) with phenol chloroform isoamyl alcohol 25:24:1 (Sigma-Aldrich) following the instructions of the manufacturer and resulting in 100 μL of RNA solution ...