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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Rat TGF beta 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... antibody levels were detected using a secondary anti-murine IgG conjugated with alkaline phosphatase and the substrate for alkaline phosphatase yellow (pNPP) liquid substrate system for ELISA (cat. P7998, Sigma). After 30 minutes of incubation ...
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bioRxiv - Cell Biology 2021Quote: VCAM-1 in the media (1:100) from MCECs stimulated with MG was measured using commercial kits from Sigma, according to the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... The supernatant was then diluted 1:1 with sterile water and 20μL was used for glycogen measurement (Glycogen Assay Kit, Cat: MAK016, Sigma).
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bioRxiv - Cell Biology 2022Quote: RNA from different cancer cell lines was extracted using RNA isolation kit (Bio Rad) and treated with DNAse using DNAse 1 kit (AMPD1, Sigma-Aldrich). cDNA synthesis was done using a cDNA synthesis kit (Bio Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... bead-based kit (Milliplex 41-plex human cytokine panel 1, Millipore, MA, USA) on the FLEXMAP 3D system (Luminex Corporation ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were purified using anti-HA affinity matrix (Sigma; rat anti-HA clone 3F10 antibodies) and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... primary antibody followed by polyclonal anti-rat IgG secondary antibody conjugated CF-647 (Sigma, catalogue no. SAB4600186). On-chip cell imaging was carried on with a long-distance 25x objective (NA=1.1 ...
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bioRxiv - Immunology 2021Quote: ... the receptors were crosslinked by the addition of anti-mouse or anti-rat IgG Alexa Fluor (Sigma) during 15 min on ice.
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bioRxiv - Cell Biology 2021Quote: ... Western blots were done with a rat monoclonal anti-HA high-affinity antibody (clone 3F10, Millipore Sigma), a rabbit polyclonal anti-histone H3 antibody (ab1791 ...
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bioRxiv - Neuroscience 2021Quote: Rat choroid plexus (lateral and 4th) was isolated in HEPES-aCSF and stored in RNAlater (R0901, Sigma) at −80 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... IL) and Rat DIMT1 siRNA (Cat# NM_001106408 (SASI_Rn02_00218668) was from (Sigma Aldrich, St. Louis, Missouri, United States) Fluorescent probes were supplied by Molecular Probes (Rockford ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal rat E-cadherin antibody that specifically binds to the extracellular domain of E-cadherin (Sigma, U3254) was used at 1:1600 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Twenty-two rats were injected intraperitoneally (i.p.) with Ecoli LPS (250 μg/kg, serotype 0127:B8, SIGMA) diluted in sterile endotoxin-free isotonic saline 24h before LTE or sham-exposure (n= 11 per group).
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bioRxiv - Bioengineering 2022Quote: ... Cells were seeded in rat tail type I collagen (100 µg/mL, Sigma Aldrich, St. Louis, MO)-coated flasks and maintained in modified Endothelial Growth Media 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cover slides were incubated at room temperature with 10 µg/ml rat-tail collagen-I (Sigma) for 3 hours ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies were used: rat monoclonal anti-mouse total β1-integrin (clone MB1.2, MAB1997 Millipore); rat monoclonal anti-mouse β1-integrin ...
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bioRxiv - Bioengineering 2019Quote: ... Recombinant human BMP-2 (Pfizer) was reconstituted in a 0.1% solution of rat serum albumin (Sigma-Aldrich) and 4 mM HCl and mixed with alginate ...
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bioRxiv - Immunology 2021Quote: ... Rat hearts were divided into groups of K-H buffer (control, n=7) and LPS (Sigma-Aldrich, #L2880 ...
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bioRxiv - Immunology 2020Quote: ... Each rat received an intraperitoneal injection of 300 ng Pertussis toxin (Sigma-Aldrich, St. Louis, MO, USA) in 0.1 ml distilled water immediately after the subcutaneous injection and again 48hours later ...
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bioRxiv - Immunology 2020Quote: ... intracellular staining was performed by 5 min pre-incubation with rat IgG in 0.5% saponin (Sigma-Aldrich) and addition of anti-CD154 antibody and anti-cytokine antibodies for another 30 min in 0.5% saponin at RT.
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bioRxiv - Molecular Biology 2023Quote: ... hepatocytes were isolated after serial perfusion of rat liver by Hanks ‘ balanced salt solution (HBSS, Sigma, H6648) and collagenase CLS type II solution (50 mg/150 mL ...
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bioRxiv - Physiology 2022Quote: ... Cells were plated onto glass coverslips coated with collagen (type I rat tail, Sigma; 10 μg/cm2) and maintained in a humidified atmosphere of 5% CO2 and 95% air at 37 °C in Williams’ Medium E supplemented with penicillin ...
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bioRxiv - Biophysics 2022Quote: ... and then incubated for 1h in BSA-PBST with a goat anti-rat CF-568 (Sigma SAb4600086) secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... rats were fasted for 18 hours before experimentation and anesthetized with Inactin (Sigma T133; 150mg/kg i.p.). A tracheotomy was performed prior to making a midline abdominal incision to expose the ventral stomach ...
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bioRxiv - Neuroscience 2023Quote: SD rats (5-week-old males) were anaesthetized with sodium pentobarbital (Sigma, 40 mg/kg, intraperitoneal injection). The brain is quickly removed and cooled in ice-cold modified artificial cerebrospinal fluid (ACSF) ...
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bioRxiv - Immunology 2023Quote: Cell seeding on transwell inserts: 120,000 T84 cells were seeded on rat-collagen (Sigma-Aldrich #C7667-25MG) coated 6.5 mm transwell 3.0 µm Pore Polycarbonate Membrane Inserts (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... the rats were anesthetized by intraperitoneal injection of 10 mg/kg sodium pentobarbital (Sigma□Aldrich, MO, USA). Next ...
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bioRxiv - Cell Biology 2023Quote: ... The AP-AKI rat model was created by retrograde injection of sodium taurocholate (Sigma□Aldrich, MO, USA) into the pancreatic duct ...
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bioRxiv - Developmental Biology 2022Quote: Pregnant rats were intraperitoneally injected with poly I:C (10 mg/kg body weight, P1530-25MG, Sigma-Aldrich) on gd 13.5 ...
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bioRxiv - Neuroscience 2023Quote: ... rats underwent three conditioning trials to associate a 0.1% saccharin solution (Sigma Aldrich, Missouri, in tap water) with drug injection ...
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bioRxiv - Neuroscience 2023Quote: Neonatal Wistar rats were administered either vehicle (phosphate buffered saline; PBS) or cisplatin (Sigma-Aldrich; 0.3mg/kg) via intraperitoneal injection delivered on 2 occasions ...
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bioRxiv - Physiology 2024Quote: ... Rats were then given neuromuscular block with pancuronium bromide (2 mg/kg; Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2024Quote: Rats were deeply anesthetized with isoflurane and transcardially perfused with cold phosphate-buffered saline (PBS, Sigma, USA), followed by cold 4% paraformaldehyde (PFA ...
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bioRxiv - Immunology 2021Quote: ... 50 μl/well of 3,3’-5,5’-tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (T040, Sigma-Aldrich Co. LLC., St. Louis, MO) was added to the plate ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Immunology 2020Quote: ... Absolute OVA-specific IgG1 concentration was determined for each sample by ELISA as described above using OVA-14 (Sigma, MO USA) as a standard and equalized across all samples ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2019Quote: ... The resulting hybridomas were screened by ELISA for reactivity with STm whole cells and purified LPS (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were washed 4 times by PBST and then 100 µL of 2000-fold diluted anti-strep-HRP (Millipore Sigma) were added into each well for another 1 hr at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Biotinylated IFX fragments were immobilised on a streptavidin-coated plate and binding of the six mouse monoclonal antibodies was tested by ELISA and detected with an alkaline-phosphatase-conjugated anti-mouse secondary antibody (Sigma-Aldrich) as previously described43 ...
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bioRxiv - Immunology 2022Quote: ... The HIV status of all participants was determined by fourth generation enzyme linked-immunosorbent assay (ELISA) testing and HIV RNA plasma viral load (Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Immunology 2023Quote: ... We confirmed the specificity of our IgA ELISA and non-cross reactivity toward IgG using purified human IgG and IgA (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype control were diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
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bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype were control diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasma was collected from blood during the GTT to determine insulin levels by ELISA (EZRMI-13K, EMD Millipore Corporation, Burlington, US). Mice were sacrificed at day 7 after a 3h fast ...
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bioRxiv - Microbiology 2022Quote: ... The fraction of proteins >1 kDa was obtained by dialysis using the Mini Dialysis Kit 1 kDa (Sigma #GE80-6483-94). All kits were applied according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: RAS and RAC activity were measured using a RAF-1 RBD and PAK-1 PDB pull-down assay kits respectively (Cat#17218 and Cat#14325, Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Acetaldehyde content was further quantified using the supernatant of WT and ΔmftG grown in HdB-Tyl supplemented with 10 g L−1 ethanol alone and 10 g L−1 glucose combined with 10 g L−1 ethanol for 48 h as described in the acetaldehyde Assay Kit (Sigma-Aldrich) by the manufacturers.