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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... then seeded into 12 mm diameter culture inserts with 8 μm pores (Millipore, Bellerica, MA). After gelation ...
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bioRxiv - Cancer Biology 2024Quote: THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, P8139) at 100 ng/ml for 24h in 6 well plates (1.5×106 cells/well ...
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bioRxiv - Microbiology 2020Quote: Hep-G2 cells were obtained from the American type culture collection (ATCC; HB-8065) and were cultured in Eagle’s Minimum Essential Medium (EMEM; Sigma) supplemented with 10% Foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... One replicate of each type of surface was left for microscopy while to the rest of three replicates 0.33% of Neutral Red (Sigma) solution in supplemented DMEM medium was added and incubated at 37 °C and 5% CO2 for 4 hours ...
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bioRxiv - Immunology 2021Quote: ... harvested and cut into pieces before digestion in complete medium supplemented with 500 U/mL type IV collagenase (Sigma) and 0.05 mg/ml DNase I (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: BHK and 293T cells were obtained from the American Type Culture Collection and maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma–Aldrich) supplemented with 10% fetal bovine serum (FBS) ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... Isolated E12.5 testes were washed with PBS and incubated with dissociation buffer containing 1mg/ml Hyaluronidase Type IV (Sigma), 5U Dispase (Corning) ...
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bioRxiv - Neuroscience 2021Quote: ... The coverslip with the dissected preparation was then placed into a custom-made open recording chamber (~1.5 ml volume) and treated with 1 mg/ml collagenase (type IV; Sigma) for ~10 s by hand pipetting ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... tissue was enzymatically digested in trypsin (1.25 mg/mL) and collagenase type I (2 mg/mL) (both from Sigma) for 45 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Individual oocytes were obtained by overnight digestion at 16°C in 0.2-0.3 mg/ml type 1A collagenase (Sigma-Aldrich), dissolved in a solution containing 82.5 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... and cells were isolated by digesting the pulp tissue with collagenase type I (3 mg/ml, Sigma-Aldrich, Germany) for 60 minutes at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... The activated fluidic channels were coated with 0.5 mg/mL collagen type IV human placenta (Sigma Aldrich, C5533-5MG) in the cell culture incubator overnight at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Chip channels were then coated with a mixture of ECM in Dulbecco’s phosphate-buffered saline (DPBS): 200μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30 μg/mL fibronectin (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies and dilutions were as follows: β-tubulin type III monoclonal (TuJ1, 1:500; Sigma, St. Louis, MO), GFAP polyclonal (1:1,000 ...
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bioRxiv - Physiology 2021Quote: The skull over the recording area was covered by sterile low melting agarose Type III (A6138, Sigma-Aldrich, Inc.) and sealed with Kwik-Cast (WPI) ...
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bioRxiv - Molecular Biology 2020Quote: Type-1 diabetes (T1D) was induced by a single intraperitoneal (i.p.) injection of streptozotocin (STZ) (185mg/kg, Sigma-Aldrich). After a six hour fast ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... through the left ventricle and then perfused with 30 mL digestion buffer (0.13 mg/mL collagenase type II (Sigma), Hank’s balanced salt solution (HyClone ...
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bioRxiv - Cell Biology 2021Quote: ... skeletal muscle samples taken from the forelimbs of mice were subjected to collagenase treatment using 0.2% collagenase type I (Sigma). Mononuclear cells were incubated with APC (allophycocyanin ...
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bioRxiv - Biophysics 2020Quote: ... Type I solution from rat tail (Cat. Nr. C3867) and fibronectin (Cat. Nr. F1141) were purchased from Sigma-Aldrich.
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bioRxiv - Cancer Biology 2022Quote: ... Minced tissues were incubated in digestion medium (Collagenase type II 5 mg/ml [GIBCO], Dispase 5 mg/ml [GIBCO], Y-27632 10.5 µM [Sigma] ...
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Computational and functional characterization of the PI(4,5)P2 binding site of the TRPM3 ion channelbioRxiv - Biophysics 2022Quote: ... then bags of ovaries were surgically collected from and rotated with 0.1-0.3 mg/ml type 1A collagenase (Sigma-Aldrich) at 16 °C overnight in OR2 buffer containing 82.5 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Material was minced finely with scissors and digested in collagenase-containing medium (DMEM with 3.2 mM CaCl2, 1.5 mg/ml collagenase type II (C6885, Sigma-Aldrich), 10% FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... The differentiated cells were then cultured in RPMI B27+ until digestion at 15 days using 0.2% collagenase type I (Sigma) in 20% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and digested for 30 minutes at 37°C in DMEM containing collagenase type IV (200 units/ml; Sigma-Aldrich) and DNase I (100 units/ml ...
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bioRxiv - Bioengineering 2022Quote: ... a 10% (w/v) gelatin solution was first obtained by dissolving gelatin from porcine skin type A (Sigma-Aldrich) in phosphate buffer saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... epididymal and inguinal adipose tissues were digested for 20-30 min at 37°C with collagenase type II (Sigma). Cell suspensions were filtered through a sieve ...
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bioRxiv - Physiology 2020Quote: ... but scaled down to the size of small zebrafish hearts and using slightly higher enzyme concentrations (1 mg/mL collagenase Type IA and 0.67 mg/mL Trypsin IV both from Sigma). A blunt-ended syringe needle (34 gauge ...
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bioRxiv - Microbiology 2020Quote: ... 1 wt% melted agarose with ultra-low gelling temperature (Type IX-A, A2576, Sigma-Aldrich, Saint Louis, MO, USA) and cells ...
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bioRxiv - Biochemistry 2020Quote: ... RF cloning was also used to introduce the pseudo-wild-type SOD1 gene into the pRFSDuet-1 plasmid (Novagen). The mutant SOD1 genes were amplified with primers containing restriction sites for NdeI and AgeI and then inserted into the pRFSDuet-1 plasmid using the In-Fusion® HD cloning kit after digesting the plasmid with the same enzymes ...
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bioRxiv - Physiology 2021Quote: Total RNA was extracted from gastrocnemius muscles from wild-type and HSA-NRTN muscles using TRI reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... the samples were gently cut into small pieces and incubated with 0.05 mg/ml collagenase type I (Sigma, USA) for one 1h to digest the epididymal fat ...
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bioRxiv - Developmental Biology 2020Quote: Wild-type ICR females were super-ovulated by sub-cutaneous injection of pregnant mare’s serum gonadotropin (PMSG) (Sigma-Aldrich) (5 units ...
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bioRxiv - Cell Biology 2020Quote: ... on collagen coated plastic (50 μg/cm2 of rat tail collagen Type IV (SERVA, 47256.01) in 0.02N acetic acid(Sigma; 537020)) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293 cells were seeded at 8,000-10,000 cells/cm2 on type 1 collagen (10 μg/cm2, Sigma-Aldrich) coated 35 mm polystyrene dishes (Falcon ...
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bioRxiv - Microbiology 2020Quote: ... this LB grown library was replica plated onto minimal MCLMAN media (30) plates supplemented with 0.5% Type III porcine gastric mucin (Sigma). Transposon insertion mutants that were qualitatively defective for growth compared to neighboring transposon insertion mutants were marked as deficient for mucin utilization for further investigation ...
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bioRxiv - Molecular Biology 2021Quote: Wild-type or mutated pMAL-c2x-malE-L375-his10 plasmids were transformed into Escherichia coli strain BL21 (EMD Millipore) for subsequent growth in LB broth supplemented with 50 μg/ml carbenicillin and 0.2% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Then the gel was washed twice with PBS and coated with 2 ml of 0.2 mg ml−1 rat tail collagen type I (Millipore) at 4 °C overnight.
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bioRxiv - Cell Biology 2021Quote: ... For assessment of hydrodynamic radius, purified motor domains (wild-type, jordan, shaker-2) were concentrated by centrifugation (10’000 MWCO; Amicon, EMD-Millipore) and directly analyzed using size exclusion chromatography (see below) ...
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bioRxiv - Immunology 2020Quote: ... Digestion of the colon tissue was performed in RPMI/10% FCS containing 0.4mg/ml type VIII collagenase (Sigma Aldrich) and 40μg/ml DNase I (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... and the retina was mounted on a nitrocellulose membrane (type AAWB, 0.8 micron pore size, Millipore, Burlington, MA, USA) and fixed by immersion in 4% paraformaldehyde for 30 mins ...
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bioRxiv - Biochemistry 2022Quote: ... that causes Weill-Marchesani Syndrome (WMS)[34] was digested overnight at 4 °C in digestion buffer (0.1 mg/ml collagenase Type 1a (Sigma) in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... Brain micro-vessels were retrieved by backflushing with 5 ml of MD131 and were incubated for 2 h at 36.5 ºC in an orbital shaker with a digestion cocktail containing 1mg/ml of collagenase type IV (Millipore), 10 μg/ml DNAse (Roche ...
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bioRxiv - Physiology 2023Quote: ... and 1% L-glutamine for 24 hours in 96-wells plates pre-coated with collagen Type I (Sigma Aldrich).
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bioRxiv - Cell Biology 2023Quote: BM cells were obtained by flushing femur and tibia bones from 8w age of wide type mice with Dulbecco’s Modified Eagle Medium (DMEM, Sigma) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... or plantaris muscles were harvested and incubated in high-glucose DMEM (HyClone Laboratories) containing 0.2% (EDL muscle) or 0.4% (plantaris muscle) collagenase type I (Sigma-Aldrich) at 37°C in a cell culture incubator ...
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bioRxiv - Cancer Biology 2023Quote: Tumor cell lines were established from fresh tumor fragments digested in a mix of collagenase type I (Sigma-Aldrich) and DNAse (F ...
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bioRxiv - Immunology 2023Quote: ... homogenized and digested in 2.5 mL of the enzyme solution [1 mg/mL Hyaluronidase Type IV-S (Sigma-Aldrich), 0.05 mg/mL Liberase TM (LIBTM-RO ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼30% CD90+ stromal cells were dissociated at 15 days using human cardiac digestion buffer: 0.2% collagenase type I (Sigma) in 20% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: ... The suspended cells in DMEM were mixed with rat tail-derived type I collagen in 0.1% acetic acid and 2X DMEM (Sigma). After neutralizing the pH with sodium hydroxide ...