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Citations for Millipore Sigma :
1751 - 1800 of 1800 citations for TMB Substrates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Blots were washed again 3 times for 10 min each and developed using Immobilon Luminata Crescendo Western HRP substrate (Millipore, Cat#WBLUR0500). Western blots were imaged and quantified using ImageLab (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... Membranes were again washed with TBS-T for 3 times and were incubated with Immobilon Forte Western HRP substrate (WBLUF0500, EMD Millipore Corporation). Images were captured using AI600 Imager from GE Healthcare Life Science.
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bioRxiv - Cell Biology 2024Quote: ... and ABTS substrate solution (water containing 0.54mg/mL w/v 2’2-Azinobis (3-ethylbenzthiazoline Sulfonic Acid) diammonium salt (Sigma-Aldrich, Cat#A-1888), and 0.1M Citric Acid and 0.03% v/v Hydrogen Peroxide (H2O2) ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed five times for 10 minutes each in TBS-T and detected with Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma, WBKLS0500) on Pro Signal Blotting film (Genesee Scientific ...
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bioRxiv - Biophysics 2023Quote: ... produced from γ-secretaseenzymatic reactions with WT or L36F Notch-based substrate was isolated by immunoprecipitation with anti-FLAG M2 beads (Sigma-Aldrich) in 10 mM MES (2-(4-morpholino ...
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bioRxiv - Cancer Biology 2023Quote: NSG mice bearing subcutaneous transplants of Hopx-MACD reporter cells were dosed with 100 µl of 30 mM of AkaLumine-HCl substrate resuspended in PBS (#808350, Millipore Sigma, TokeOni) and imaged on an IVIS Lumina II (Perkin Elmer)(23).
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bioRxiv - Immunology 2023Quote: ... was added for 1h at RT followed by incubation with OPD (o-phenylenediamine dihydrochloride) revelation substrate for 10 min (Sigma-Aldrich, #P8287). Absorbances were analyzed at 492 vs 620 nm on an ELISA plate reader (Berthold).
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bioRxiv - Biochemistry 2024Quote: ... and LysC (Lysyl Endopeptidase 129-02541, Wako Chemicals) in a 1:50 protease to substrate ratio in ammonium bicarbonate buffer (Sigma, 50 mM). The next day ...
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bioRxiv - Biochemistry 2024Quote: The activity of the enzyme was estimated by measuring the rate of hydrolysis of the fluorescent substrate L-leucine-7-amido-4-methyl coumarin (Leu-AMC, Sigma–Aldrich, L2145) followed at 460 nm (excitation at 380 nm ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were visualized using Fusion Fx (Vilber Lourmat, Marne-la-Vallée, France) with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Billerica, MA, USA). Protein levels were quantified using ImageJ and normalized to the β-actin levels.
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bioRxiv - Molecular Biology 2020Quote: ... CsCl fractions containing needle complexes and the substrate were pooled and incubated with Anti-FLAG M2 magnetic beads (M8823, Sigma Aldrich/Merck KGaA) prewashed in FR3 buffer (10 mM Tris-HCl ...
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bioRxiv - Biophysics 2019Quote: ... was followed by monitoring the conversion of the fluorogenic substrate Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC, Sigma-Aldrich, Sintra, Portugal) to 7-amino-4-methylcoumarin (AMC ...
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bioRxiv - Bioengineering 2020Quote: GO and RGO substrates were placed in 12-well plates and Fibronectin (FN) from human plasma (Sigma-Aldrich, 10 µg/ml in PBS) solution was added and allowed to adsorb for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... 20 μL of (GlcNAc)6 was added in a final concentration of 10 μM to trigger ROS production in the absence or presence of 100 μL of effector protein in a final concentration of 50 μM in measuring solution containing 100 μM L-012 substrate (FUJIFILM, Neuss, Germany) and 40 μg/mL horseradish peroxidase (Sigma-Aldrich, Missouri, USA). Chemiluminescence measurements were taken every minute over 30 min in a CLARIOstar microplate reader (BMG LABTECH ...
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bioRxiv - Neuroscience 2021Quote: ... Protein signal was developed using enhanced chemiluminescence according to the manufacturer’s instruction (Immobilon® Forte Western HRP substrate, Merck Millipore Ltd., VIC, Australia) and visualised using the ChemiDocTM XRS system (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... before processing for western blotting according to our previously published protocol.75 We used the Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore, Cat# WBKLSO500) to visualize the signals and detected on the ChemiDoc MP imager (Bio-Rad) ...
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bioRxiv - Genetics 2020Quote: Exopolyphosphatase activity of wild-type PRUNE1 on medium and long chain PolyP substrates (consisting of 45, 65 and 700 orthophosphate monomers, Sigma S4379, Sigma S6253, Kerafast EUI002) was assessed by comparing to S ...
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bioRxiv - Immunology 2022Quote: ... a developing buffer was prepared as follows: one tablet of each 3,3’-Diaminobenzidin peroxidase-substrate (0.7 mg/ml, Sigma-Aldrich, gold and silver tablets) was dissolved in 5 ml distilled water ...
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bioRxiv - Immunology 2019Quote: Chitinase activity was analyzed by using the substrate and standard solutions of the fluorimetric chitinase assay kit (Sigma-Aldrich Chemie GmbH, Munich, Germany). Substrates 4-MU-(GlcNAc)2 (Exo-Chitinase activity ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Microbiology 2020Quote: ... DMSO was used at a final concentration of 0.05%.3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride and BCIP®/NBT solution (premixed) were procured from Sigma-Aldrich (St. Louis, USA).
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bioRxiv - Neuroscience 2022Quote: The catalytic activity of NAGLU was determined by hydrolysis of the fluorogenic substrate 4-methylumbelliferyl-N-acetyl-α-glucosaminide (EMD Millipore, Burlington, MA) with a final concentration of 0.1 mM substrate in the incubation mixture as described previously50 ...
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bioRxiv - Physiology 2024Quote: ... vesicles were rapidly separated from the substrate medium by filtration on mixed ester filters (0.45 μm pore size, Millipore Corporation, Bedford, MA, USA) and washed with 3 × 2 ml of PBS ...
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bioRxiv - Cell Biology 2023Quote: The gels images were acquired using enhanced chemiluminescence (ECL) by treating the membranes in plastic wrap with Immobilon Forte Western HRP Substrate (Millipore. Cat. No.: WBLUF0100) and imaging on Amersham Imager 600 or iBright imaging system ...
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bioRxiv - Cell Biology 2023Quote: ... PDMS substrates were rinsed with sterile water three times and coated with 50 µg/mL of fibronectin (F4759, Sigma-Aldrich, St. Louis, MO) diluted in water for one hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The plates were then washed three times with 0.05% Tween-PBS and incubated for 20 min in a solution containing o-phenylenediamine·2HCl (OPD) substrate (Sigma-Aldrich, St. Louis, MO) diluted in substrate buffer and 30% H2O2 ...
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bioRxiv - Microbiology 2023Quote: Plasmin activity was measured using the specific plasmin chromogenic substrate D-Val-Leu-Lys 4-nitroanilide dihydrochloride (Millipore-Sigma, Burlington, MA, USA, #V0882). All proteins were prepared in phosphate-lysine buffer (10mM potassium phosphate ...
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bioRxiv - Physiology 2023Quote: ... Cells were incubated with P-gp substrate calcein-acetoxymethyl ester (Ca-AM, 177831, 10−6 M, Sigma; 37°C, 5% CO2, 1 hour). Once substrate was added ...
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bioRxiv - Microbiology 2023Quote: Plasmin activity was measured using the specific plasmin chromogenic substrate D-Val-Leu-Lys 4-nitroanilide dihydrochloride (Millipore-Sigma, Burlington, MA, USA, #V0882). All proteins were prepared in phosphate-lysine buffer (10mM potassium phosphate ...
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
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bioRxiv - Cancer Biology 2024Quote: ... a substrate of the bone marrow sample was cultured enabling MSCs growth in α-MEM ((StableCell Minimum Essential Medium Eagle – α modification, Sigma #M6199) supplemented with human Platelet Lysate (hPL ...
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bioRxiv - Neuroscience 2024Quote: ... 24.75 µl of activity assay buffer (0.25% w/v taurocholic acid in citrate/phosphate buffer) containing 2 mM 4-methylumbelliferyl-β-D-glucopyranoside substrate (4MU-Gluc; Sigma-Aldrich, # M3633) in activity assay buffer ...
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bioRxiv - Microbiology 2021Quote: The relative neuraminidase activity of the oseltamivir-treated and untreated obese and wild-type-derived viruses was measured by using the fluorogenic substrate MUNANA (Sigma-Aldrich, St Louis, MO). Two-fold dilutions of day 1 ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... The plates were washed using ELISA washing solution followed by addition of substrate (4-Nitrophenyl phosphate disodium salt hexahydrate, Sigma-Aldrich, 1 mg/ml), 150 µl/well ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Pharmacology and Toxicology 2022Quote: To confirm activity of the HIV protease a positive control was run alongside all drug-release experiments using an HIV Protease fluoregenic substrate (Sigma Aldrich Cat. No H0790).
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bioRxiv - Biochemistry 2022Quote: The proteins were visualized by incubating membranes for 30 minutes in BCIP®/NBT Alkaline Phosphatase Substrate (B5655, SIGMAFAST™ BCIP®/NBT, Sigma-Aldrich). The intensities of the detected bands were analyzed with a FluorChem 8800 Imager (Alpha Innotech ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Microbiology 2020Quote: ... A substrate solution consisting of one tablet each of o-phenylenediamine and urea hydrogen peroxide (No. P9187; Sigma-Aldrich Corp., St. Louis, MO, USA) in 10 mL ddH2O ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Immunology 2021Quote: ... which were diluted 1:100 in triplicate into 5 mL fresh CHG media containing either 100 μM of the corresponding substrate (either LCA [Sigma] or 3-oxoLCA [Steraloids]). Cultures were grown for 48 hours at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... the growth medium contained 50 mM TMA as the growth substrate and agar solidified HS + TMA media was obtained by adding 1.5% w/v agar (Sigma-Aldrich, St. Louis, MO, USA). To select for transformants ...
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bioRxiv - Genetics 2020Quote: Exopolyphosphatase activity of wild-type PRUNE1 on medium and long chain PolyP substrates (consisting of 45, 65 and 700 orthophosphate monomers, Sigma S4379, Sigma S6253, Kerafast EUI002) was assessed by comparing to S ...
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bioRxiv - Biochemistry 2020Quote: ... these lysates were probed with an anti-GroEL monoclonal antibody (1.10B) at 1:100 dilution and the blots were developed by BCIP/NBT-Purple Liquid Substrate System (Sigma Aldrich Inc., St. Louis, MO, USA). In addition to these strains ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with 15 μM substrate 2′ (4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-NANA) in 37°C warmed sterile filtered TBS (Sigma Aldrich; St. Louis, MO). Fluorescence intensity (excitation ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrations of mesotrypsin and bovine trypsin were determined by active site titration using p-nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) substrate (Sigma-Aldrich, St. Louis, MO, USA), which served as an irreversible inhibitor46 ...
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bioRxiv - Immunology 2021Quote: ... inhibition of CHIT1 enzyme activity was measured using fluorogenic substrate 4-methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside (4MU-GlcNAc3; Sigma-Aldrich, St Louis, MO) following hydrolysis measuring liberated fluorescent 4- in McIlvain buffer (100 mmol/l citric acid and 200 mmol/l sodium phosphate [pH 5.2] ...
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bioRxiv - Immunology 2023Quote: ... After 30 minutes incubation at room temperature the plate was washed 4 times with PBST and alkaline phosphatase substrate was added (Sigma Aldrich, S0942; St. Louis, MO). The plate was analyzed using a plate reader ...
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bioRxiv - Neuroscience 2023Quote: ... The underside of the silicon substrate of the electrode was coated with DiI dissolved in a 70% isopropanol solution in water (Sigma-Aldrich, St. Louis, MO, USA) and dried before use.
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed and incubated with the appropriate secondary antibody in 5% non-fat dry milk in PBST for 2h at RT and revealed in Chemiluminescent HRP Substrate (Sigma Aldrich, Saint Quentin Fallavier, France).